LOCUS       sequence-47336-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Shcherbakova DM, Hink MA, Joosen L, Gadella TW, Verkhusha VV
  TITLE     An Orange Fluorescent Protein with a Large Stokes Shift for 
            Single-Excitation Multicolor FCCS and FRET Imaging.
  JOURNAL   J Am Chem Soc. 2012 May 9;134(18):7913-23. doi: 10.1021/ja3018972
  PUBMED    22486524
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "J Am Chem Soc"; date: "2012-05-9- 9"; volume: "134"; issue: "18"; 
            pages: "7913-23"
COMMENT     Sequence Label: pH2B-LSSmOrange
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             83..460
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      473..482
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     892..911
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcca ccatgccaga gccagcgaag tctgctcccg ccccgaaaaa
      121 gggctccaag aaggcggtga ctaaggcgca gaagaaaggc ggcaagaagc gcaagcgcag
      181 ccgcaaggag agctattcca tctatgtgta caaggttctg aagcaggtcc accctgacac
      241 cggcatttcg tccaaggcca tgggcatcat gaattcgttt gtgaacgaca ttttcgagcg
      301 catcgcaggt gaggcttccc gcctggcgca ttacaacaag cgctcgacca tcacctccag
      361 ggagatccag acggccgtgc gcctgctgct gcctggggag ttggccaagc acgccgtgtc
      421 cgagggtact aaggccatca ccaagtacac cagcgctaag gatccaccgg tcgccaccat
      481 ggtgagcaag ggcgaggaga ataacatggc catcatcaag gagttcatgc gcttcaaggt
      541 gcgcatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
      601 cccctacgag ggctttcaga ccgttaagct gaaggtgacc aagggtggcc ccctgccctt
      661 cgcctgggac atcttgtccc ctcagttcac ctacggctcc aaggcctacg tgaagcaccc
      721 cgccgacatc cccgactacc tcaagctgtc cttccccgag ggcttcaagt gggagcgcgt
      781 gatgaacttc gaggacggcg gcgtggtgac cgtgactcag gactcctccc tgcaggacgg
      841 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
      901 gcagaagaag accatgggca tggaggcctc ctccgagcgg atgtac
//
