LOCUS       sequence-47337-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Shcherbakova DM, Hink MA, Joosen L, Gadella TW, Verkhusha VV
  TITLE     An Orange Fluorescent Protein with a Large Stokes Shift for 
            Single-Excitation Multicolor FCCS and FRET Imaging.
  JOURNAL   J Am Chem Soc. 2012 May 9;134(18):7913-23. doi: 10.1021/ja3018972
  PUBMED    22486524
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "J Am Chem Soc"; date: "2012-05-9- 9"; volume: "134"; issue: "18"; 
            pages: "7913-23"
COMMENT     Sequence Label: pLSSmOrange-mKate2 caspase-3 biosensor
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(126..824)
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     regulatory      821..830
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc tagagtcgcg
      121 gccgctcatc tgtgccccag tttgctaggg aggtcgcagt atctggccac agccacctcg
      181 tgctgctcga cgtaggtctc tttgtcggcc tccttgattc tttccagtct tctgtccaca
      241 tagtagacgc cgggcatctt gaggttctta gcgggtttct tggatctgta tgtggtcttc
      301 aagttgcaga tcaggtggcc cccgcccacg agcttcaggg ccatgtcggc tctgccttcc
      361 aggccgccgt cagcggggta cagggtctcg gtggaggcct cccagccgag tgttttcttc
      421 tgcatcacag ggccgttgga tgggaagttc acccctctga tcttgacgtt gtagatgagg
      481 cagccgtcct ggaggctggt gtcctgggta gcggtcagca cgcccccgtc ttcgtatgtg
      541 gtgactctct cccatgtgaa gccctcgggg aaggactgct taaagaagtc ggggatgccc
      601 tgggtgtggt tgatgaaggt tttgctgccg tacatgaagc tggtagccag gatgtcgaag
      661 gcgaagggga gagggccgcc ctcgaccgcc ttgattctca tggtctgggt gccctcgtan
      721 ggcttgcctt cgccctcgga tgtgcacttg aagtggtggt tgttcacggt gccctccatg
      781 tacagcttca tgtgcatgtt ctccttaatc agctcgctca ccatggtggc gaccggtgaa
      841 ccagaaccac caagcttatc aacttcatca gaaccagaac caccgaattc cttgtacagc
      901 tcgtccatgc cg
//
