LOCUS       sequence-473378-       11659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express LgBiT-AR/AR-SmBiT Fusion Protein in Mammalian Cells under a 
            CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11659)
  AUTHORS   Promega
  TITLE     Promega Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LgBiT-AR/AR-SmBiT Bidirectional Vector
FEATURES             Location/Qualifiers
     source          1..11659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    8..89
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(3618..3636)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3619..3636)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     enhancer        3756..4059
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4060..4263
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4214..4234
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          4399..4531
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4514..4533
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     4575..4594
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4575..4593
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7541..7559)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    7596..7717
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7607..7626)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        7962..8319
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7962..8153
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7985..8005)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8170..8305
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8350..9387
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             9366..9374
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    9411..9459
                     /note="synthetic polyadenylation signal"
     promoter        9615..9719
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9720..9788,9789..10580)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9938..9957)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(10738..11326)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10818..10837)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gacgtcggca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
       61 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
      121 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
      181 tggctgatta tgatccgcgg ccgcaatacg tcgacgttat cagctgactt cgtacgagag
      241 cctaggtgtt tctagaggat ccccgggtac cgagcccgaa ttcgtttaaa cctgggtgtg
      301 gaaatagatg ggcttgactt tcccagaaag gatcttgggc acttgcacag agatgatctc
      361 tgccatcatt tccggaaagt ccacgctcac catgtgtgac ttgattagca ggtcaaaagt
      421 gaactgatgc agctctctcg caataggctg cacggagtcc aggagcttgg tgagctggta
      481 gaagcgtctt gagcaggatg tgggattttt tcttttgcat gcaatgatac gatcgagttc
      541 cttgatgtag ttcattcgaa gttcatcaaa gaatttttga tttttcagcc catccactgg
      601 aataatgctg aagagtagca gtgctttcat gcacaggaat tcctgggggg tgatttggag
      661 ccatccaaac tcttgagaga ggtgcctcat tcggacacac tggctgtaca tccgggactt
      721 gtgcatgcgg tactcattga aaaccagatc aggggcgaag tagagcatcc tggagttgac
      781 attggtgaag gatcgccagc ccatggcaaa caccatgagc cccatccagg agtactgaat
      841 gacagccatc tggtcgtcca cgtgtaagtt gcggaagcca ggcaaggcct tggcccactt
      901 gaccacgtgt acaagctgtc tctctcccag ttcattgagg ctagagagca aggctgcaaa
      961 ggagtcgggc tggttgttgt cgtgtccagc acacactaca cctggctcaa tggcttccag
     1021 gacattcaga aagatgggct gacattcata gccttcaatg tgtgacactg tcagcttctg
     1081 ggttgtctcc tcagtggggc tggtggtgct ggaagcctct ccttcctcct gtagtttcag
     1141 attaccaagt ttcttcagct tccgggctcc cagagtcatc cctgcttcat aacatttccg
     1201 aagacgacaa gatggacaat ttttccttcg gaatttatca atagtgcaat catttctgct
     1261 ggcgcacagg tacttctgtt tcccttcagc ggctcttttg aagaagacct tgcagcttcc
     1321 acatgtgaga gctccatagt gacacccaga agcttcatct ccacagatca ggcaggtctt
     1381 ctggggtgga aagtaatagt caatgggcaa aacatggtcc ctggcagtct ccaaacgcat
     1441 gtccccgtaa ggtccggagt agctatccat ccaggggccc atttcgcttt tgacacaagt
     1501 gggactggga tagggcactc tgctcaccat gccgccaggg taccacacat caggtgcggt
     1561 gaagtcgctt tcctggcccg ccagcccctg agggggccga gtgtagccgt agggggctac
     1621 agctcccgcc tcgccgccgc cgccgccgcc gccgccgccg ccgccgccgc cgccgccgcc
     1681 gccgccacca cccccaccac caccacacgg tccatacaac tggccttctt cggctgtgaa
     1741 gagagtgtgc caggatgagg aagcggcggc tgagggtgac ccagaaccgg gtcccgctgc
     1801 acccgcgcca tgcaggctcg ccaggtcccc atagcggcac tgcgccgccg cagccgccca
     1861 ggcgctgccg tagtccagcg ggttctccag cttgatgcga gcgtggggat ggggaggcgg
     1921 cggagggggc ggcggtccgg ccagagccag tggaaagttg tagtagtcgc gactctggta
     1981 cgcagctgcc tcgtccagtg ctccggactt gtagagagac agggtagacg gcagttcaag
     2041 tgtcccggag ctccctgctg cagcgctgcc agagcagcct aggctctcgc cttctagccc
     2101 tttggtgtaa cctcccttga aaggggaata ctcagcagta tcttcagtgc tcttgcctgc
     2161 gctgtcgtct agcagagaac ctttgcattc ggccaatggg gcacaaggag tgggacgcac
     2221 agcgggtgga actcccaaaa gtggggcgta catgcaatcc ccccgaagct gttcccctgg
     2281 actcagatgc tccaacgcct ccacacccag gcccatggac accgacactg ccttacacaa
     2341 ctccttggcg ttgtcagaaa tggtcgaagt gccccctaag taattgtcct tggaggaagt
     2401 gggagccccc gaggcttccc tcgctctccc gctgctgctg ccttcggata ctgcttcctg
     2461 ctgctgttgc tgaaggagtt gcatggtgct ggcctcgctc aggatgtctt taaggtcagc
     2521 ggagcagctg cttaagccgg ggaaagtggg gcccagcagg gacaacgtgg atggggcagc
     2581 tgagtcatcc tcgtccggag gtgctggcag ctgctgcggc agccccttgc tggcggccac
     2641 ggcggctcca ggctctggga cgcaacctct ctcggggtgg cactccaggg ccgactgcgg
     2701 ctgtgaaggt tgctgttcct catccaggac caggtagcct gtggggcctc tacgatgggc
     2761 ttggggagaa ccatcctcac cctgctgctg ctgctgctgc ctggggctag tctcttgctg
     2821 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgcag
     2881 cagcagcaaa ctggcgccgg gaggtgctgc gctcgcggcc tctgggtgcc tggggcccgg
     2941 gttctggatc acttcgcgca cgctctggaa cagattctgg aaagctcctc ggtaggtctt
     3001 ggacggcggc cgagggtaga cccttcccag ccctaactgc acttccatgg cgatcgcacc
     3061 gctcgagcct ccacctccgc tcccgccacc accggagctc ccactgttga tggttactcg
     3121 gaacagcatg gagccgtcgg gggtgatcag gcgctcgtcg ataattttgt tgccgttcca
     3181 cagggtccct gttacagtga tctttttgcc gtcgaacacg gcgatgcctt catacggccg
     3241 tccgaaatag ttcagcatgt tcggcgtaac cccgtcgatt accagtgtgc catagggcag
     3301 gatcacctta aagtgatgat catccacagg gtacaccacc ttaaacacct cttcgatctg
     3361 ggccatttgg tcggcgctca gaccttcata cgggatgatg acatggatgt cgatcttcag
     3421 ggcattttca ccgctccgga caatcctttg gatcggagtt acggacacgg cgagattctg
     3481 cagcaaactg gacacacctc cctgttcaag gacttggtcc aggttgtagg cggctgtctg
     3541 ttcccagtcc ccaacgaaat cttcgagtgt gaagaccatg gtgagcgcta gctgcaaaaa
     3601 gaacaagtag cttgtattct atagtgtcac ctaaatgggc gatctgacgg ttcactaaac
     3661 cagctctgct tatataggcc tccctggcgc gccactagtt attaatagta atcaattacg
     3721 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     3781 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     3841 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     3901 gcccacttgg cagtacatca agtgtatcat atgccaagtc cgccccctat tgacgtcaat
     3961 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttacggga ctttcctact
     4021 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     4081 accaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     4141 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaataac
     4201 cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     4261 gctggtttag tgaaccgtca gatcactaga agctttattg cggtagttta tcacagttaa
     4321 attgctaacg cagtcagtgc ttctgacaca acagtctcga acttaagctg cagaagttgg
     4381 tcgtgaggca ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag
     4441 aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt
     4501 actgacatcc actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct
     4561 taaggctaga gtattaatac gactcactat agggctagcg atcgccatgg aagtgcagtt
     4621 agggctggga agggtctacc ctcggccgcc gtccaagacc taccgaggag ctttccagaa
     4681 tctgttccag agcgtgcgcg aagtgatcca gaacccgggc cccaggcacc cagaggccgc
     4741 gagcgcagca cctcccggcg ccagtttgct gctgctgcag cagcagcagc agcagcagca
     4801 gcagcagcag cagcagcagc agcagcagca gcagcagcaa gagactagcc ccaggcagca
     4861 gcagcagcag cagggtgagg atggttctcc ccaagcccat cgtagaggcc ccacaggcta
     4921 cctggtcctg gatgaggaac agcaaccttc acagccgcag tcggccctgg agtgccaccc
     4981 cgagagaggt tgcgtcccag agcctggagc cgccgtggcc gccagcaagg ggctgccgca
     5041 gcagctgcca gcacctccgg acgaggatga ctcagctgcc ccatccacgt tgtccctgct
     5101 gggccccact ttccccggct taagcagctg ctccgctgac cttaaagaca tcctgagcga
     5161 ggccagcacc atgcaactcc ttcagcaaca gcagcaggaa gcagtatccg aaggcagcag
     5221 cagcgggaga gcgagggaag cctcgggggc tcccacttcc tccaaggaca attacttagg
     5281 gggcacttcg accatttctg acaacgccaa ggagttgtgt aaggcagtgt cggtgtccat
     5341 gggcctgggt gtggaggcgt tggagcatct gagtccaggg gaacagcttc ggggggattg
     5401 catgtacgcc ccacttttgg gagttccacc cgctgtgcgt cccactcctt gtgccccatt
     5461 ggccgaatgc aaaggttctc tgctagacga cagcgcaggc aagagcactg aagatactgc
     5521 tgagtattcc cctttcaagg gaggttacac caaagggcta gaaggcgaga gcctaggctg
     5581 ctctggcagc gctgcagcag ggagctccgg gacacttgaa ctgccgtcta ccctgtctct
     5641 ctacaagtcc ggagcactgg acgaggcagc tgcgtaccag agtcgcgact actacaactt
     5701 tccactggct ctggccggac cgccgccccc tccgccgcct ccccatcccc acgctcgcat
     5761 caagctggag aacccgctgg actacggcag cgcctgggcg gctgcggcgg cgcagtgccg
     5821 ctatggggac ctggcgagcc tgcatggcgc gggtgcagcg ggacccggtt ctgggtcacc
     5881 ctcagccgcc gcttcctcat cctggcacac tctcttcaca gccgaagaag gccagttgta
     5941 tggaccgtgt ggtggtggtg ggggtggtgg cggcggcggc ggcggcggcg gcggcggcgg
     6001 cggcggcggc ggcggcggcg gcgaggcggg agctgtagcc ccctacggct acactcggcc
     6061 ccctcagggg ctggcgggcc aggaaagcga cttcaccgca cctgatgtgt ggtaccctgg
     6121 cggcatggtg agcagagtgc cctatcccag tcccacttgt gtcaaaagcg aaatgggccc
     6181 ctggatggat agctactccg gaccttacgg ggacatgcgt ttggagactg ccagggacca
     6241 tgttttgccc attgactatt actttccacc ccagaagacc tgcctgatct gtggagatga
     6301 agcttctggg tgtcactatg gagctctcac atgtggaagc tgcaaggtct tcttcaaaag
     6361 agccgctgaa gggaaacaga agtacctgtg cgccagcaga aatgattgca ctattgataa
     6421 attccgaagg aaaaattgtc catcttgtcg tcttcggaaa tgttatgaag cagggatgac
     6481 tctgggagcc cggaagctga agaaacttgg taatctgaaa ctacaggagg aaggagaggc
     6541 ttccagcacc accagcccca ctgaggagac aacccagaag ctgacagtgt cacacattga
     6601 aggctatgaa tgtcagccca tctttctgaa tgtcctggaa gccattgagc caggtgtagt
     6661 gtgtgctgga cacgacaaca accagcccga ctcctttgca gccttgctct ctagcctcaa
     6721 tgaactggga gagagacagc ttgtacacgt ggtcaagtgg gccaaggcct tgcctggctt
     6781 ccgcaactta cacgtggacg accagatggc tgtcattcag tactcctgga tggggctcat
     6841 ggtgtttgcc atgggctggc gatccttcac caatgtcaac tccaggatgc tctacttcgc
     6901 ccctgatctg gttttcaatg agtaccgcat gcacaagtcc cggatgtaca gccagtgtgt
     6961 ccgaatgagg cacctctctc aagagtttgg atggctccaa atcacccccc aggaattcct
     7021 gtgcatgaaa gcactgctac tcttcagcat tattccagtg gatgggctga aaaatcaaaa
     7081 attctttgat gaacttcgaa tgaactacat caaggaactc gatcgtatca ttgcatgcaa
     7141 aagaaaaaat cccacatcct gctcaagacg cttctaccag ctcaccaagc tcctggactc
     7201 cgtgcagcct attgcgagag agctgcatca gttcactttt gacctgctaa tcaagtcaca
     7261 catggtgagc gtggactttc cggaaatgat ggcagagatc atctctgtgc aagtgcccaa
     7321 gatcctttct gggaaagtca agcccatcta tttccacacc caggtttctc aaggcagttc
     7381 aggtggtggc gggagcggag gtggaggctc gagcggtgtg accggctacc ggctgttcga
     7441 ggagattctg taaggccgcg actctagagt cgacctgcag gcatgcaagc tgatccggct
     7501 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
     7561 taaccccttg gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca
     7621 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     7681 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     7741 tatgtttcag gttcaggggg agatgtggga ggttttttta agcaagtaaa acctctacaa
     7801 atgtggtaaa atcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt
     7861 tctccttacg catctgtgcg gtatttcaca ccgcatacgc ggatctgcgc agcaccatgg
     7921 cctgaaataa cctctgaaag aggaacttgg ttaggtacct tctgaggcgg aaagaaccag
     7981 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     8041 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     8101 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     8161 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8221 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8281 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcgattc ttctgacact
     8341 agcgccacca tgaagaagcc cgaactcacc gctaccagcg ttgaaaaatt tctcatcgag
     8401 aagttcgaca gtgtgagcga cctgatgcag ttgtcggagg gcgaagagag ccgagccttc
     8461 agcttcgatg tcggcggacg cggctatgta ctgcgggtga atagctgcgc tgatggcttc
     8521 tacaaagacc gctacgtgta ccgccacttc gccagcgctg cactacccat ccccgaagtg
     8581 ttggacatcg gcgagttcag cgagagcctg acatactgca tcagtagacg cgcccaaggc
     8641 gttactctcc aagacctccc cgaaacagag ctgcctgctg tgttacagcc tgtcgccgaa
     8701 gctatggatg ctattgccgc cgccgacctc agtcaaacca gcggcttcgg cccattcggg
     8761 ccccaaggca tcggccagta cacaacctgg cgggatttca tttgcgccat tgctgatccc
     8821 catgtctacc actggcagac cgtgatggac gacaccgtgt ccgccagcgt agctcaagcc
     8881 ctggacgaac tgatgctgtg ggccgaagac tgtcccgagg tgcgccacct cgtccatgcc
     8941 gacttcggca gcaacaacgt cctgaccgac aacggccgca tcaccgccgt aatcgactgg
     9001 tccgaagcta tgttcgggga cagtcagtac gaggtggcca acatcttctt ctggcggccc
     9061 tggctggctt gcatggagca gcagactcgc tacttcgagc gccggcatcc cgagctggcc
     9121 ggcagccctc gtctgcgagc ctacatgctg cgcatcggcc tggatcagct ctaccagagc
     9181 ctcgtggacg gcaacttcga cgatgctgcc tgggctcaag gccgctgcga tgccatcgtc
     9241 cgcagcgggg ccggcaccgt cggtcgcaca caaatcgctc gccggagcgc agccgtatgg
     9301 accgacggct gcgtcgaggt gctggccgac agcggcaacc gccggcccag tacacgaccg
     9361 cgcgctaagg aggtaggtcg agtttagact ctagccatca cgatggccgc aataaaatat
     9421 ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagcg ataaggatcc
     9481 tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc atccggggtc
     9541 agcaccgttt ctgcggactg gctttctacg taatggtttc ttagacgtca ggtggcactt
     9601 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     9661 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     9721 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     9781 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     9841 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     9901 aagaacgttt tccaatgatg agcactttca aagttctgct atgtggcgcg gtattatccc
     9961 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    10021 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    10081 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaactatcg
    10141 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    10201 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    10261 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    10321 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    10381 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    10441 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    10501 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    10561 cactgattaa gcattggtaa ttcgaaatga ccgaccaagc gacgcccaac cggtatcagc
    10621 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    10681 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    10741 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    10801 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    10861 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    10921 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    10981 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    11041 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    11101 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    11161 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    11221 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    11281 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tttcaagaag atcctttgat
    11341 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    11401 gagattatca aaaaggatct tcacctagat ccttttatag tccggaaata caggaacgca
    11461 cgctggatgg cccttcgctg ggatggtgaa accatgaaaa atggcagctt cagtggatta
    11521 agtgggggta atgtggcctg taccctctgg ttgcataggt attcatacgg ttaaaattta
    11581 tcaggcgcga ttgcggcagt ttttcgggtg gtttgttgcc atttttacct gtctgctgcc
    11641 gtgatcgcgc tgaacgcgt
//
