LOCUS       sequence-47338-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Shcherbakova DM, Hink MA, Joosen L, Gadella TW, Verkhusha VV
  TITLE     An Orange Fluorescent Protein with a Large Stokes Shift for 
            Single-Excitation Multicolor FCCS and FRET Imaging.
  JOURNAL   J Am Chem Soc. 2012 May 9;134(18):7913-23. doi: 10.1021/ja3018972
  PUBMED    22486524
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "J Am Chem Soc"; date: "2012-05-9- 9"; volume: "134"; issue: "18"; 
            pages: "7913-23"
COMMENT     Sequence Label: pLSSmOrange-mKate2 caspase-3 biosensor
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             48..755
                     /codon_start=1
                     /product="monomeric orange fluorescent protein with a large
                     Stokes shift (Shcherbakova et al., 2012)"
                     /label=LSSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTVKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGMEASSERMYPEDGALK
                     GEDKLRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     461..480
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      816..825
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgccaccatg gtgagcaagg
       61 gcgaggagaa taacatggcc atcatcaagg agttcatgcg cttcaaggtg cgcatggagg
      121 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
      181 gctttcagac cgttaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
      241 tcttgtcccc tcagttcacc tacggctcca aggcctacgt gaagcacccc gccgacatcc
      301 ccgactacct caagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
      361 aggacggcgg cgtggtgacc gtgactcagg actcctccct gcaggacggc gagttcatct
      421 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
      481 ccatgggcat ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
      541 aggacaagct caggctgaag ctgaaggacg gcggccacta cacctccgag gtcaagacca
      601 cctacaaggc caagaagccc gtgcagttgc ccggcgccta catcgtcgac atcaagttgg
      661 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
      721 gccactccac cggcggcatg gacgagctgt acaaggaatt cggtggttct ggttctgatg
      781 aagttgataa gcttggtggt tctggttcac cggtcgccac catggtgagc gagctgatta
      841 aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt
      901 gcacatccga
//
