LOCUS       sequence-47341-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Shcherbakova DM, Hink MA, Joosen L, Gadella TW, Verkhusha VV
  TITLE     An Orange Fluorescent Protein with a Large Stokes Shift for 
            Single-Excitation Multicolor FCCS and FRET Imaging.
  JOURNAL   J Am Chem Soc. 2012 May 9;134(18):7913-23. doi: 10.1021/ja3018972
  PUBMED    22486524
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "J Am Chem Soc"; date: "2012-05-9- 9"; volume: "134"; issue: "18"; 
            pages: "7913-23"
COMMENT     Sequence Label: pLSSmOrange-N1
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(125..835)
                     /codon_start=1
                     /product="monomeric orange fluorescent protein with a large
                     Stokes shift (Shcherbakova et al., 2012)"
                     /label=LSSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTVKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGMEASSERMYPEDGALK
                     GEDKLRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(403..422)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      832..841
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    843..923
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
       61 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct agagtcgcgg
      121 ccgcttactt gtacagctcg tccatgccgc cggtggagtg gcggccctcg gcgcgttcgt
      181 actgttccac gatggtgtag tcctcgttgt gggaggtgat gtccaacttg atgtcgacga
      241 tgtaggcgcc gggcaactgc acgggcttct tggccttgta ggtggtcttg acctcggagg
      301 tgtagtggcc gccgtccttc agcttcagcc tgagcttgtc ctcgcccttc agggcgccgt
      361 cctcggggta catccgctcg gaggaggcct ccatgcccat ggtcttcttc tgcattacgg
      421 ggccgtcgga ggggaagttg gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct
      481 gcagggagga gtcctgagtc acggtcacca cgccgccgtc ctcgaagttc atcacgcgct
      541 cccacttgaa gccctcgggg aaggacagct tgaggtagtc ggggatgtcg gcggggtgct
      601 tcacgtaggc cttggagccg taggtgaact gaggggacaa gatgtcccag gcgaagggca
      661 gggggccacc cttggtcacc ttcagcttaa cggtctgaaa gccctcgtag gggcggccct
      721 cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg cgcaccttga
      781 agcgcatgaa ctccttgatg atggccatgt tattctcctc gcccttgctc accatggtgg
      841 cgaccggtgg atcccgggcc cgcggtaccg tcgactgcag aattcgaagc ttgagctcga
      901 gatctgagtc cggtagcgct agcggatctg acgg
//
