LOCUS       sequence-47343-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Shcherbakova DM, Hink MA, Joosen L, Gadella TW, Verkhusha VV
  TITLE     An Orange Fluorescent Protein with a Large Stokes Shift for 
            Single-Excitation Multicolor FCCS and FRET Imaging.
  JOURNAL   J Am Chem Soc. 2012 May 9;134(18):7913-23. doi: 10.1021/ja3018972
  PUBMED    22486524
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "J Am Chem Soc"; date: "2012-05-9- 9"; volume: "134"; issue: "18"; 
            pages: "7913-23"
COMMENT     Sequence Label: pBAD-LSSmOrange
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(4..714)
                     /codon_start=1
                     /product="monomeric orange fluorescent protein with a large
                     Stokes shift (Shcherbakova et al., 2012)"
                     /label=LSSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTVKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGMEASSERMYPEDGALK
                     GEDKLRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(282..301)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(724..741)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(842..861)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 ttcttacttg tacagctcgt ccatgccgcc ggtggagtgg cggccctcgg cgcgttcgta
       61 ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg tccaacttga tgtcgacgat
      121 gtaggcgccg ggcaactgca cgggcttctt ggccttgtag gtggtcttga cctcggaggt
      181 gtagtggccg ccgtccttca gcttcagcct gagcttgtcc tcgcccttca gggcgccgtc
      241 ctcggggtac atccgctcgg aggaggcctc catgcccatg gtcttcttct gcattacggg
      301 gccgtcggag gggaagttgg tgccgcgcag cttcaccttg tagatgaact cgccgtcctg
      361 cagggaggag tcctgagtca cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc
      421 ccacttgaag ccctcgggga aggacagctt gaggtagtcg gggatgtcgg cggggtgctt
      481 cacgtaggcc ttggagccgt aggtgaactg aggggacaag atgtcccagg cgaagggcag
      541 ggggccaccc ttggtcacct tcagcttaac ggtctgaaag ccctcgtagg ggcggccctc
      601 gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgc gcaccttgaa
      661 gcgcatgaac tccttgatga tggccatgtt attctcctcg cccttgctca ccatagatct
      721 gccatggtga tggtgatggt ggctccccat ggttaattcc tcctgttagc ccaaaaaacg
      781 ggtatggaga aacagtagag agttgcgata aaaagcgtca ggtaggatcc gctaatctta
      841 tggataaaaa tgctatggca tagcaaagtg tgacgccgtg caaataatca atgtggactt
      901 ttctgccgtg attatagaca cttttgttac
//
