LOCUS       sequence-473439-        8712 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A piggybac-based cloning vector containing dual sgRNAs for spCas9 
            and CAG promoter-driven nuclear-localized Clover-T2A-BSR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8712)
  AUTHORS   Okubo C, Nakamura M, Sato M, Shichino Y, Mito M, Takashima Y, 
            Iwasaki S, Takahashi K
  TITLE     EIF3D safeguards the homeostasis of key signaling pathways in human 
            primed pluripotency.
  JOURNAL   Sci Adv. 2025 Apr 11;11(15):eadq5484. doi: 10.1126/sciadv.adq5484. 
            Epub 2025 Apr 9.
  PUBMED    40203091
REFERENCE   2  (bases 1 to 8712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-04-11"; volume: "11(15):eadq5484. doi"; pages: " 
            10.1126/sciadv.adq5484. Epub 2025 Apr 9"
COMMENT     Sequence Label: PB-2G-CNCB
FEATURES             Location/Qualifiers
     source          1..8712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1371..1619
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1371..1391
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1542..1561
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     protein_bind    complement(1953..1986)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     1990..2010
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     enhancer        2330..2633
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          2913..3929
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3853..3875
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3937..3956
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3994..4014
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4018..4038
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4042..4062
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(4078..4080,4081..4083,4084..4794)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(4123..4144)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     4731..4752
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4795..4848
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    5284..5405
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5321..5340)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5375..5394
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6168..6202
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6693..6709)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6693..6709)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6706..6728)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6717..6733
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6741..6747,6748..6765,6766..6771))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6786..6807
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6922..6939)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7093..7681)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7173..7192)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7852..8643,8644..8712))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8475..8494
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggcccgat gaggcccttg gagggcctat
     1381 ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa
     1441 ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat
     1501 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg
     1561 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
     1621 cgtcttcgaa tgaagacacg tttcagagct aagcacaaga gtgcatagca agttgaaata
     1681 aggctagtcc gtttacaact tgaaaaagtg gcacccgagt cgggtgcttt ttttgctcga
     1741 atctacactc agctcgagga tccgacgcgc catctctagg cccgcgccgg ccccctcgca
     1801 cggacttgtg ggagaagctc ggctactccc ctgccccggt taatttgcat ataatatttc
     1861 ctagtaacta tagaggctta atgtgcgata aaagacagat aatctgttct ttttaatact
     1921 agctacattt tacatgatag gcttggattt ctataacttc gtatagcata cattatacga
     1981 agttataaac agcacaaaag gaaactcacc ctaactgtaa agtaattgtg tgttttgaga
     2041 ctataagtat cccttggaga accaccttgt tggggagacg cgttacgtct ccgtttaaga
     2101 gctatgctgg aaacagcata gcaagtttaa ataaggctag tccgttatca acttgaaaaa
     2161 gtggcaccga gtcggtgctt tttttctcga gtactaggat ccattaggcg gccgcgtgga
     2221 taaccgtatt accgccatgc atcccgggtg actgactgag cggccgcact agttattaat
     2281 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     2341 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     2401 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     2461 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     2521 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     2581 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg
     2641 tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt
     2701 atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg
     2761 cgccaggsgg ggsggggsgg ggsgrggggs ggggsggggs grggsggrga ggtgcggcgg
     2821 cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc
     2881 ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg
     2941 tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc
     3001 cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat
     3061 gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt
     3121 tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt
     3181 gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc
     3241 tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct
     3301 gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg
     3361 gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc
     3421 cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg
     3481 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg grgggctcgg
     3541 gggaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc
     3601 attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg
     3661 cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt
     3721 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc
     3781 cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac
     3841 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc
     3901 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt
     3961 ctcatcattt tggcaaagaa ttccgccacc atgccaaaaa agaagagaaa ggtagatcca
     4021 aaaaagaaga gaaaggtaga tccaaaaaag aagagaaagg tacacgtgtc aacgcgtatg
     4081 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     4141 gacgtaaacg gccacaagtt cagcgtccgc ggcgagggcg agggcgatgc caccaacggc
     4201 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     4261 gtgaccacct tcggctacgg cgtggcctgc ttcagccgct accccgacca catgaagcag
     4321 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catctctttc
     4381 aaggacgacg gtacctacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     4441 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     4501 ctggagtaca acttcaacag ccacaacgtc tatatcacgg ccgacaagca gaagaacggc
     4561 atcaaggcta acttcaagat ccgccacaac gttgaggacg gcagcgtgca gctcgccgac
     4621 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     4681 ctgagccatc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     4741 ctggagttcg tgaccgccgc cgggattaca catggcatgg acgagctgta caaggagggc
     4801 agaggaagcc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaaaaccttc
     4861 aacatctccc agcaggatct agaattagta gaagtagcga cagagaagat tacaatgctt
     4921 tatgaggata ataaacatca tgtgggagcg gcaattcgta cgaaaacagg agaaatcatt
     4981 tcggcagtac atattgaagc gtatatagga cgagtaactg tttgtgcaga agccattgcg
     5041 attggtagtg cagtttcgaa tggacaaaag gattttgaca cgattgtagc tgttagacac
     5101 ccttattctg acgaagtaga tagaagtatt cgagtggtaa gtccttgtgg tatgtgtagg
     5161 gagttgattt cagactatgc accagattgt tttgtgttaa tagaaatgaa tggcaagtta
     5221 gtcaaaacta cgattgaaga actcattcca ctcaaatata cccgaaatta agttaactaa
     5281 ctaaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     5341 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     5401 tcttatcatg tctggaattg actcaaatga tgtcaattag tctatcagaa gctcatctgg
     5461 tctcccttcc gggggacaag acatccctgt ttaatattta aacagcagtg ttcccaaact
     5521 gggttcttat atcccttgct ctggtcaacc aggttgcagg gtttcctgtc ctcacaggaa
     5581 cgaagtccct aaagaaacag tggcagccag gtttagcccc ggaattgact ggattccttt
     5641 tttagggccc attggtatgg ctttttcccc gtatcccccc aggtgtctgc aggctcaaag
     5701 agcagcgaga agcgttcaga ggaaagcgat cccgtgccac cttccccgtg cccgggctgt
     5761 ccccgcacgc tgccggctcg gggatgcggg gggagcgccg gaccggagcg gagccccggg
     5821 cggctcgctg ctgcccccta gcgggggagg gacgtaatta catccctggg ggctttgggg
     5881 gggggctgtc cctgatatct ataacaagaa aatatatata taataagtta tcacgtaagt
     5941 agaacatgaa ataacaatat aattatcgta tgagttaaat cttaaaagtc acgtaaaaga
     6001 taatcatgcg tcattttgac tcacgcggtc gttatagttc aaaatcagtg acacttaccg
     6061 cattgacaag cacgcctcac gggagctcca agcggcgact gagatgtcct aaatgcacag
     6121 cgacggattc gcgctattta gaaagagaga gcaatatttc aagaatgcat gcgtcaattt
     6181 tacgcagact atctttctag ggttaatcta gctgcatcag gatcatatcg tcgggtcttt
     6241 tttccggctc agtcatcgcc caagctggcg ctatctgggc atcggggagg aagaagcccg
     6301 tgccttttcc cgcgaggttg aagcggcatg gaaagagttt gccgaggatg actgctgctg
     6361 cattgacgtt gagcgaaaac gcacgtttac catgatgatt cgggaaggtg tggccatgca
     6421 cgcctttaac ggtgaactgt tcgttcaggc cacctgggat accagttcgt cgcggctttt
     6481 ccggacacag ttccggatgg tcagcccgaa gcgcatcagc aacccgaaca ataccggcga
     6541 cagccggaac tgccgtgccg gtgtgcagat taatgacagc ggtgcggcgc tgggatatta
     6601 cgtcagcgag gacgggtatc ctggctggat gccgcagaaa tggacatgga taccccgtga
     6661 gttacccggc gggcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6721 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     6781 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6841 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6901 cgtattgggc gctcttgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     6961 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7021 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7081 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7141 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7201 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7261 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7321 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7381 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7441 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7501 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     7561 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7621 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7681 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     7741 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     7801 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     7861 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     7921 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     7981 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8041 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8101 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8161 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8221 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8281 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8341 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8401 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8461 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8521 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8581 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     8641 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     8701 ttgaatactc at
//
