LOCUS       sequence-47344-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Shcherbakova DM, Hink MA, Joosen L, Gadella TW, Verkhusha VV
  TITLE     An Orange Fluorescent Protein with a Large Stokes Shift for 
            Single-Excitation Multicolor FCCS and FRET Imaging.
  JOURNAL   J Am Chem Soc. 2012 May 9;134(18):7913-23. doi: 10.1021/ja3018972
  PUBMED    22486524
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "J Am Chem Soc"; date: "2012-05-9- 9"; volume: "134"; issue: "18"; 
            pages: "7913-23"
COMMENT     Sequence Label: pBAD-LSSmOrange
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             60..77
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..797
                     /codon_start=1
                     /product="monomeric orange fluorescent protein with a large
                     Stokes shift (Shcherbakova et al., 2012)"
                     /label=LSSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTVKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGMEASSERMYPEDGALK
                     GEDKLRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     500..519
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(843..860)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 ctctactgtt tctccatacc cgttttttgg gctaacagga ggaattaacc atggggagcc
       61 accatcacca tcaccatggc agatctatgg tgagcaaggg cgaggagaat aacatggcca
      121 tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg ctccgtgaac ggccacgagt
      181 tcgagatcga gggcgagggc gagggccgcc cctacgaggg ctttcagacc gttaagctga
      241 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cttgtcccct cagttcacct
      301 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacctc aagctgtcct
      361 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
      421 tgactcagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
      481 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggcatg gaggcctcct
      541 ccgagcggat gtaccccgag gacggcgccc tgaagggcga ggacaagctc aggctgaagc
      601 tgaaggacgg cggccactac acctccgagg tcaagaccac ctacaaggcc aagaagcccg
      661 tgcagttgcc cggcgcctac atcgtcgaca tcaagttgga catcacctcc cacaacgagg
      721 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
      781 acgagctgta caagtaagaa ttcgaagctt ggctgttttg gtggatgaga gaagattttc
      841 agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa tttgctggcg
      901 gcagtagcgc ggtg
//
