LOCUS       sequence-473445-       12212 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression in bacterial cells for protein purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12212)
  AUTHORS   Kuchay S, Duan S, Schenkein E, Peschiaroli A, Saraf A, Florens L, 
            Washburn MP, Pagano M
  TITLE     FBXL2- and PTPL1-mediated degradation of p110-free p85beta 
            regulatory subunit controls the PI(3)K signalling cascade.
  JOURNAL   Nat Cell Biol. 2013 May;15(5):472-80. doi: 10.1038/ncb2731. Epub 
            2013 Apr 21.
  PUBMED    23604317
REFERENCE   2  (bases 1 to 12212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2731"; 
            journalName: "Nat Cell Biol"; date: "2013-05"; volume: "15"; issue: 
            "5"; pages: "472-80"
COMMENT     Sequence Label: pUC18 HA PTPL1
FEATURES             Location/Qualifiers
     source          1..12212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2105..2131)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(2188..2391)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2217..2237)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2392..2771
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(2932..2949)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2932..2948)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2941..2963)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(3089..3544)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3226..3247)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3438..3457
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3571..3675
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3676..3744,3745..4536)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3894..3913)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4707..5295
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5196..5215
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5449..5466
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5583..5604
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5619..5624,5625..5642,5643..5649)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5657..5673
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5662..5684
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5681..5697
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5681..5697
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5711..5730
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5711..5729
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5768..6097)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5782..5917
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6082..6102
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    6126..6748
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     6530..6549
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 gcaataagga agtctcatca tcacagtgca tcctttcctc caaaatatct tttcgaagct
       61 gaaggtaata ctgatgacac gtcagagtat gttgtattag actaacatca tccataaaaa
      121 atttaattct gaaaaacaaa gtaaaattaa cagtggcttt ggtctttttc tttggttctt
      181 ctttccatcc ctctggggcc actttggtta attttaagtc aggatcaaca aagaaatatt
      241 cattatcttt gagggtagct aaagcaaaca aatgatgctc tactaagcca atatgtgcca
      301 caaccatatc aaacacatct ttacatatag ttttggtatc acaggtcagt tccagtcttt
      361 gcccgttcag aagcattatg tttactttcc ttcggttatc ctcattcttc cctttcttag
      421 taagaataga ccgtggcaaa tccaaagata taaatggttc aattgtcatt ttcacaaact
      481 cagggccaaa gaaattcctc agttttcttt gagtcatggc tgtttctagg gcaatttctc
      541 ttaacggatc cctggtgatg tcaagcatgg acgctttgat tgtgtctcct ggatataggc
      601 tcaaccgaga ctgtctaagg gccattttgg cttgtagctg catcagttct tcctcttgcc
      661 gttttagcat gatctcttga ttaattaagt tgccttcaaa gggtgtttca tattgtctgc
      721 tcggtctctg tgactggcct tgacttaagg tttcatctac ccctgggaga ccactgctgg
      781 attcaaacct ctttgaggct tcacttcttc tcacttgtct gaaatctgat tcagaggaaa
      841 taatagatgg actttcactg gaggtactaa agacatcacc atgataagtt ttgtatcttc
      901 gaactggttc ttctacattc atggcttccc tcagaacctg aagtttcttt tgtctctctc
      961 ggattcggtc caaagcactt gatattgctg aggaggtggg caattctcga gtgtgatata
     1021 ttggatccat tttctgaggg ccaaagatat ccaaggctat acttccatct gagtatcttg
     1081 cctccttttt tctaggagta gttgaagtcc gcactgttac tgcctcaggg tgacaacgac
     1141 gatatgtggc agtctctcct gaagagaagt ctctgtcagc tgtacaaagc aagtccatgg
     1201 atgaagccca gatcttcttc ttagaaagca catcaatgcc agggatgggt tttttttctg
     1261 ggccactagt tttgaactgg taaggggaga atgtattttc agaatcttca cgtccagaat
     1321 tatctgataa aatgtccttg aaataattct catcttgtgt atctttgatg gacagaaatc
     1381 ccatagattt actaagccct ttgttaagaa aggtctgatg agagagtgga ggcttttgta
     1441 tgtctagtac atcagaagta gagcttcttc ctgttggtaa tccttttcct cgcaatcgat
     1501 ctcgaatagc ctggcttcga tcaggctttt gttcactgtt acaggaaagc tgatctgtcc
     1561 cagaaagatt tcccagaacc agttttacca agtgtttcac gtaggaaaat gagggtgcac
     1621 aattgctatt cctaatgtgg gcactgcaag catccagcac agtccgaaca gaaactcgag
     1681 cgtaaataac atcctcacac attccaagca gtatgctgtt gagatgatct ccaagcttaa
     1741 taggttggct ctgaggcact tcataatcag ccccccaata cagtgtcatt ccaagagaat
     1801 aaatgtggat cttttcaaca tctgagagag aagttagtga ctgattttga agaacctctg
     1861 gtgcagtgaa tgctcgaaga tcctgattgg aaatattttc atctgtaaat gacacactac
     1921 cagatggcag caacagcaga gaccatggag aaatgatgaa gccaagggca gcaggatcag
     1981 ctaggcttac ttttctgaat aattcttgga gactttcagc actttgattt aatacagccc
     2041 atatttcttc ctcctgaagt ggtccacccc gaacctccag ggcctcagct agtgacacgt
     2101 gcatggcgta atctggcaca tcgtatgggt acatggtggc atcgatacgc gtggtaccag
     2161 atctgaattc tgacggttca ctaaacgagc tctgcttata tagacctccc accgtacacg
     2221 cctaccgccc atttgcgtca acggggcggg gttattacga cattttggaa agtcccgttg
     2281 attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg
     2341 tgagtcaaac cgctatccac gcccattggt gtactgccaa aaccgcatca ccatggtaat
     2401 agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccgtaagg tcatgtactg
     2461 ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg gacttggcat
     2521 atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg
     2581 tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg
     2641 gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa
     2701 ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagtcaa
     2761 taatcaatgt caacatggcg gtcatattgg acatgagcca atataaatgt acatattatg
     2821 atatagatac aacgtatgca atggccaata gccaatattg atttatgcta tataaccaat
     2881 gactaatatg gctaattgcc aatattgatt caatgtatag atcagcttgg cactggccgt
     2941 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     3001 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     3061 acagttgcgc agcctgaatg gcgaatggaa attgtaagcg ttaatatttt gttaaaattc
     3121 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     3181 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     3241 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     3301 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     3361 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     3421 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     3481 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     3541 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3601 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3661 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     3721 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     3781 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     3841 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     3901 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     3961 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     4021 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     4081 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4141 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4201 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4261 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4321 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4381 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4441 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     4501 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     4561 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     4621 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4681 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4741 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4801 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     4861 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4921 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4981 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5041 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5101 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5161 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5221 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5281 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5341 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     5401 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     5461 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     5521 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     5581 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     5641 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
     5701 attacgaatt taatacgact cactataggg aattaacttt ctggtttttc agttcctcga
     5761 ggagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     5821 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     5881 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
     5941 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg
     6001 actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     6061 gggagcctgg ggactttcca caccctaact gacacacatt ccacagccaa gctggccgcg
     6121 tacccaattc aacaggcatc tactgagtgg acccaacgca tgagaggaca gtgccaagca
     6181 agcaactcaa atgtcccacc ggttgggcat ggccaggtag cctatgctgt gtctggacgt
     6241 cctcctgctg gtatagttat tttaaaatca gaaggacagg gaagggagca gtggttcacg
     6301 cctgtaatcc cagcaatttg ggaggccaag gtgggtagat cacctgagat taggagttgg
     6361 agaccagcct ggccaatatg gtgaaacccc gtctctacca aaaaaacaaa aattagctga
     6421 gcctggtcat gcatgcctgg aatcccaaca actcgggagg ctgaggcagg agaatcgctt
     6481 gaacccagga ggcggagatt gcagtgagcc aagattgtgc cactgcactc cagcttggtt
     6541 cccaatagac cccgcaggcc ctacaggttg tcttcccaac ttgccccttg ctccatacca
     6601 cccccctcca ccccataata ttatagaagg acacctagtc agacaaaatg atgcaactta
     6661 attttattag gacaaggctg gtgggcactg gagtggcaac ttccagggcc aggagaggca
     6721 ctggggaggg gtcacaggga tgccacccgg gatcctctag agtcgacggt atcgataagc
     6781 ttgatatcga attccgttgc tgtcgatgca tgttgtatga acttgctgct ctgtgatctt
     6841 tattttggat agagagcagg agtctgctgg aggttaagga gagaaatgga aatgcatcca
     6901 gaggctcttt tcatgtcact tcagaagctg aggctgctgt ttttgctctt cttctgcttg
     6961 aagacgtgtc aggacataaa ggatgacttg atagcagaaa atatattgat cctctgtctg
     7021 aaccattccg tgtctttgta gtctcatgca gcgcaccaaa tcagagatgt caaaatcaag
     7081 atcctgactg attaatccca gaaccacatc tatgcaaatc agggtccctg aacgtccaat
     7141 gccagcactg cagtgcgtaa tgattgggcc tgatctgtgg atgtgtctca tgtaggagat
     7201 aaaagtaagc agatcatctg gttgagaagg tgtatcatgg tctggccagg cagtgaaatt
     7261 cagatgagaa atatggcgca cctctctggt ctgaatatct tcaagggtca ttgccctcac
     7321 cacaaagccc ttcagctgct gcattctcac aagagccagt cgaagtctgt tgctgaccat
     7381 tgttgttttg cctaggatgt tgggccaata gcgctggcat ttgatttttt ctccttctac
     7441 ttcttgagtc atcatggcta tcactgtgga tttttgctcc caaatcatct gccagaagtc
     7501 tccaacagtt gtaggcagtg gtccttggca ggcaatgtaa acgaactctt ctttcccaac
     7561 tggtatctta atgaagctgg cattgatata gccaccttca tctccaagag gcactcttgt
     7621 agcatcatag ggaagtatat ttttatatct gttcttcctt ctgttttcct tagtttgccc
     7681 aattagacac tgatccaaag gttttaattc ttgaagattc tccagctcct tagaaggaat
     7741 tccttgatct agcaaacccc gcaggactcg aatgactgat tttaagttgg cacccgtgta
     7801 tttaccagag ggaagcactt tgacgacagg gagtacagcg agctcatcgt ttgtcagaaa
     7861 ggaatgatct ttatcagaat cttcatggtt tgttctctct attggcaact catcatttcc
     7921 ccatgttatt tcatcatcat cagtcttctt ttcttgtggc ttctgaatta agctttcact
     7981 ttttactttt tcttcacaat attcttcaca gccattcatt ttggtggcct cagtaaaata
     8041 ctcaggtaaa ggtgaaccat cgcagtctgt atcttctgtt ctctcttctg ataacttccc
     8101 attcatcttt aatgtacctg gaccacagga gtctcctgct gcattatttt cgtttaaaag
     8161 attctgggct tcctcatcca caacatccag cagagactgg ataacttcag cttcttggga
     8221 atcatcataa atgtcatctt cttgtatata agattctttg ggcagagtca agtttggtga
     8281 tccctttatc tgataagtag aacattttcc tttggggtac gatatttcat ttatttcttc
     8341 cccagcaacc gtggagaatg aatcattagg agtgagggca ggctggctgc aagaccccac
     8401 actgtaggaa ccattgcctt tggttgactt tggagctgaa acagcagacc tctttgagct
     8461 ggggaccact ggaagatcat ttcttgttgc tttcaatacc aatgaaggaa gtgacatgtc
     8521 taatgctctg ttaacttcct ccaaggtcat tccttgtgtg ctgactccgt tcacataatg
     8581 gatgacatct aatagctgga gattaccctc aatggctgcg acggaccttg gattaatatc
     8641 actaatatat accacttgat aaaggctgtc atgacctcca cataaggaaa aacccaactc
     8701 ctctttgttg cacgttagtg ttatgtccgg tagcaaatga ggcaacattg gtattctggg
     8761 taattctaga actcgtccaa taactaatct gactgttttg gatgcagccc ggagcagatt
     8821 aactgcatct gtatgagtca tattagtaac atctgtatca ttaaccttta tgagccggtc
     8881 cccaggtttt agccttccat cacttttggc tggatcctgt atgacatcat gaacataaca
     8941 accaattctc tgattgcctt tggttactgt aaaacccagg cttccttttt ctgatttaat
     9001 tagggtaatg aggagttcta cttccagttc aaagtcttca aggtgatttt ccaagtcatt
     9061 tttcaaaggt gtccagtttt cttgtctagt tggttcagaa atgtgatgta tatgatactt
     9121 atccatcgaa ctagaggaag cagattctga ttggggttga taactctgcc caggagccat
     9181 atccggtggt agaggggaag gattactgtc ctcaaactct ggtttattgg gaattttctg
     9241 aggatagtaa aacatggtac aaatggtatc ttcttgactc ttgggtgctt catgatgact
     9301 ctgtgcctgt gataccatgt tgcttagagt ctgatgcaaa gctgaactcc aggtcgaatt
     9361 tgatatgttt gctggagctg tcactaagtc atcttctcca ctcccactgc tgtcactgta
     9421 actgtctctt ctggatgggg acttgcactg ttttttgctt ttgtctgaca tttcattttc
     9481 atctgagctg gtgctttgct ccacacatga tggctgagaa gagtctttac tgctgtttgg
     9541 aagtacttgt gctggagact gaagtggggt caaaagcgca gtatcaattt ccggtagcac
     9601 accaggtgga ggtctgcaga gaagcaagaa tacttctgga gcagttcccc tgagagcaga
     9661 tatgacttcc tgctgagata gtcctttcaa agaggctcca ttcactttca agataacatc
     9721 tcctacatca atttttccac tttctgctgc tggctgtcca ggaaagagct ttttaaccct
     9781 tactatgctg gcattaattt gctccggtat aagattatct tctcgagaaa aactgaatcc
     9841 tagacctgag ctatttttaa ataattttac ctcaaatgta ttttcttcag tgacaaagct
     9901 gtagtctttg acctgagttg ttttcttcac tttttctggg ccttgacctt gggcattctg
     9961 atctgaaagg gtacactgtg gggttaccgg gacatgttct ttagatgttg gagattgtcc
    10021 cttttctaat aacagatgaa ccacctgtcc tgtatttctc agtgtttcca cagcttgctt
    10081 atgggtggct ccttctagac taactccatt gacagctagg acgcgatcac ctttgtgaat
    10141 tctaccatca gactctgctg ctccctgggg aataacagct ttcacataaa tgccaccatg
    10201 tctgacactc gtattcacac ctcccgtgac acttatcccc aagctgttat catttttagc
    10261 cagttcaacc tcaaagatat ctccaggctt aggaggtgat gaagaaaaag ttttaaaatt
    10321 catcttgttg gatgtattca ctgaagagga agattcctgt tttggtgttt gatgatcctg
    10381 actgctggag taagtggctt catccatgtc tgaatctcca cggtctgagt aatcagttac
    10441 atcagaaatg cctggctttt tagttttgct ttggttacta tcagtgaaag tctctttctc
    10501 ggtggctttg gatattacag atggggaagg gctgccatgc tgtgattcct gccaggtttg
    10561 gtcacctaaa tggctggcaa agaaaccatt gacctggctt tgagacaagc tggcactctc
    10621 agtcctggaa tcttgagaac tcaggcttcc ttcccgcagg cccccagacg gcccaaagga
    10681 gttctccgag atgtgcctca gggtaccacg tgaccagtgg tgatccttgg aagaagaatc
    10741 tatagcactg tcttgcatgt aggaagattt cttcatgtag tttttcatct cattggtgag
    10801 atgcacagga gtagaaggca ctttggatat cttttctttt ggctgagaga taacaagtgt
    10861 cacatcttca ggtgcatttt gcaaaatttc aattgcagca tggtggctga ctccctccag
    10921 actcacacta ttcacagata tcaaacggtc tcctggcttc aagcatccat ccaagtcagc
    10981 tggtcctcca ggggcaactg aactgataaa tatgcctagg tccagtcttc ccatcttctc
    11041 cccaccaata atttgaaatc ccaagccata ctttgcatct tttttcaggt tcactaaggt
    11101 gatctccctt tctggtgaag atactatgct ccatcttttg tgtagcacat ctagaacata
    11161 tgcttgccca gggtcttcaa tggatgagga gtctgattca tgtttcctcc tttcaggact
    11221 tctatttaaa ctcgcaacag actttgaatt attaagtttt gtcactcctg ccaaagattc
    11281 tgcatcagat cttgacatct ggtgagaagg ttttcccacc aactctgcaa cggtttgtgg
    11341 tgggggttcc atgttaacaa tgacattttt ccgatgtgga tagagagagg cctgactgag
    11401 atcatggtat gatttactca tctctctagg cttttcctcc catgaagctt tgtcattctt
    11461 ctcttttgaa ctgttttgca atggctggta aagcgacagc tctgagcagg atagcctctt
    11521 cagaatctca gcttgaactg ataaaggtcg aacagcaagt ttgttgaggg tgctgctggc
    11581 cagactgcca gtgctgattg ctcgtcccat attaaatcct ctaacagact ctgcttggag
    11641 attcaggctc ctaaacgaag ctctctcaat atcttgggca tcttggttgc tctgtcttgc
    11701 tctcatctgt agctggaact tatgctggta agagcagagg tgcagcaggt actggcatat
    11761 cttactgttg tctgtctgga agccatgttt tattccatct gatgtatttt gcaatgtgat
    11821 tttcttttta gaaaaagata ttttcttggt ttccctccat ggaaagcgaa ggaccaatgt
    11881 gcgcactcca ttgtgaactt caaacacaag gacaccttta gaacagactc caagcaatat
    11941 tcctgtttgt gacttcttct cagggtgcac tcggtgaaaa tgaactccat attctgtcag
    12001 tctttggcag acctttaaaa attctaactc tgtctctttt tcagaagctc ccacataggt
    12061 attatgcaat ttgggtaact cttctttgat ataggataaa tcaagtttct ccatcactct
    12121 ggcgggcaaa tagtgctcca ttctaaagta agacacacca tgaacctctg gttgataatc
    12181 tccatactca gcctggagag ccaaggatgc ca
//
