LOCUS       sequence-473449-       11472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transient overexpression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11472)
  TITLE     GMCL1 Controls 53BP1 Stability and Modulates Paclitaxel Sensitivity 
            in Cancer
REFERENCE   2  (bases 1 to 11472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCMV_53BP1IEDI_AAAA_FLAG
FEATURES             Location/Qualifiers
     source          1..11472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      410..419
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(465..488)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(504..527)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(628..647)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(629..647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(692..895)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(721..741)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        896..1275
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1447..1466
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1541..1645
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1646..1714,1715..2506)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1864..1883)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2677..3265
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3166..3185
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3419..3436
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3553..3574
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3589..3594,3595..3612,3613..3619)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3627..3643
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3632..3654
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3651..3667
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3651..3667
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    3716..3837
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3727..3746)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3781..3800
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(4011..4805)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4122..4141)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4732..4751
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4872..5201)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4886..5021
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4940..4959)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     5186..5206
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(5215..5643)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5325..5346)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5537..5556
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    5689..5913
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5902..5919
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        5939..5957
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5939..5956
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tcttctccct tctcttcctc cacagcagga gcagattcca ctgacattcc cagaacactg
       61 cttgtcctgt ttggctgagg taactgctca atgacctgac tgatggaacc acatgtgtcc
      121 aagttagaag aatccacagg gtctgcaacc ttgttttctt tcaaatgttc attgaaccca
      181 ctattaccgt ctcctcgttc ttctccagct gtttgttcag gattggacac aacatccaac
      241 acaggatttt ctttgtgcgt ctggagatta ggaaggtgtc gagatagcat actgaagtga
      301 gaaccagaat catcctctag aacctggctt tcaggctgag aatcttcaat tatcaggcaa
      361 ggagtatctt gctgagagaa atctgaatcc aactgacttc cagtagggtc catggtggcg
      421 gaaccaccac caccggaacc accaccaccg gaaccaccac cacctttatc atcatcatct
      481 ttataatccc cagatcctgg taccttgtca tcgtcgtcct tgtagtccat ggtctcgagc
      541 ggccgccagt gtgatggata tctgcagaat tccagcacac tggcggccgt tactagtgga
      601 tccgagctcg gtaccaagct tgggtctccc tatagtgagt cgtattaatt tcgataagcc
      661 agtaagcagt gggttctcta gttagccaga gagctctgct tatatagacc tcccaccgta
      721 cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc
      781 gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc
      841 cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg
      901 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt
      961 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg
     1021 gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt
     1081 gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca
     1141 atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc
     1201 ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat taataactag
     1261 tcaataatca atgtcaacgc gtatatctgg cccgtacatc gcgaagcagc gcaaaacgcc
     1321 taaccctaag cagattcttc atgcaattgt cggtcaagcc ttgccttgtt gtagcttaaa
     1381 ttttgctcgc gcactactca gcgacctcca acacacaagc agggagcaga tactggctta
     1441 actatgcggc atcagagcag attgtactga gagtgcacca taggggatcg ggagatctcc
     1501 cgatccgtcg acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     1561 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     1621 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     1681 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     1741 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     1801 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     1861 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     1921 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     1981 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     2041 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     2101 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     2161 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     2221 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     2281 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     2341 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     2401 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     2461 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     2521 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     2581 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     2641 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2701 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2761 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2821 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2881 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2941 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3001 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3061 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3121 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3181 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3241 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3301 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3361 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     3421 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     3481 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     3541 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     3601 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     3661 ctatgaccat gattacgcca agctctagct agaggtcgac ggtatacaga catgataaga
     3721 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     3781 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttggg
     3841 gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg
     3901 gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga
     3961 tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac
     4021 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
     4081 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
     4141 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag
     4201 cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc
     4261 tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
     4321 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
     4381 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
     4441 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
     4501 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
     4561 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
     4621 tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
     4681 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
     4741 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
     4801 atcatgcgaa acgatcctca tcctgtctct tgatcagatc cgaaaatgga tatacaagct
     4861 cccgggagct ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat
     4921 agctcagagg cagaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg
     4981 ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg
     5041 actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct
     5101 ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct
     5161 gctggggagc ctggggactt tccacaccct aactgacaca cattccacag aattaattcg
     5221 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     5281 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     5341 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     5401 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     5461 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     5521 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     5581 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     5641 cgtggggata ccccctagag ccccagctgg ttctttccgc ctcagaagcc atagagccca
     5701 ccgcatcccc agcatgcctg ctattgtctt cccaatcctc ccccttgctg tcctgcccca
     5761 ccccaccccc cagaatagaa tgacacctac tcagacaatg cgatgcaatt tcctcatttt
     5821 attaggaaag gacagtggga gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc
     5881 aaacaacaga tggctggcaa ctagaaggca cagtcgaggc tgatcagcga gctctagcat
     5941 ttaggtgaca ctatagaata gggcccttag tgagaaacat aatcgtgttt atattttgga
     6001 tgctgcttga atccaattct ctccccaaca atgaggcact ggatcaccca ctcttgtgac
     6061 accacaggca gctgcaatgc ttcagcacac ttcagcaccg aggctgggca tgaggggtcc
     6121 gtcaccacca catcaaatac ccctaaagca atatctttgt tatgggcact tgaatggtgc
     6181 tgcttcacag aggctgcacc accagtcatg aggatctcag accagagctc caggaagttc
     6241 tgctgttggt ctgataccaa gagtaccttc agattctgga aaggattttc acggggttgc
     6301 cagtccagaa ttctttgctc ctcaaggctg tacccagctg gcaacagata attacggtag
     6361 ttctggagct ggttggcatg gcaactatca tggacccaga catgagacac acaaggaatc
     6421 ccactggcaa ggcacaggaa gtacttccgg gttcgacaat gctgatccgc aattagaaga
     6481 cactggtaag ctgtgttaca ctgggcttca ttgaaatctt caaggatata gccagctcct
     6541 gctcgaagct gggattctgt atactgcttg ttgaaaggag gaatttccaa aaattcctcc
     6601 tcttcttcac tgcttcctgt aggaccatct ggcagtttgg agcggctggc caacttgtca
     6661 ctggttgtgg ccatggtaag gagaaatgcg tagcccagaa acaaggtctt gttgagaggc
     6721 aaaggccctc tctgctcttc cagggcagag ggttcaccgg tgttgtctcc actctcacag
     6781 gggctcacaa actctcctgc ccctactgca ccaggtttta ctgtggcaga cttgcgacct
     6841 cgcttggcag gggaccgttc ctcttcagaa gtgataagtt ttcttttgcc tgagagaact
     6901 cccatggagg cacgaggact ttctgtgatc tttcgggtag gggttgtgct gctgctactg
     6961 gaggcagtag gggtggctgg ggagctgacg ttactgcgcc gtttccgctt cccttccacc
     7021 aaattgtcta agctgatatc tgctgccttt gtaagaggtg ttactgcttc ataggggcca
     7081 agcccatact gctctctcag tctgtttcct tgctccaagg acaggatgac agccattcgc
     7141 ttataccact ttctttggcc ttctttttca atgctgtagt acagttcccc agactccttc
     7201 ctatgtcctt tcaccactcc tgcactgaaa tactcatcct ccgagagggc cgtcacttca
     7261 gtgtccagcg ggatggggtc acataacaga atgtctttgc ccaacacatc acattcgtac
     7321 ccatcatcaa agagcaattt atacttccca gctccgacat ctcgtgtgat tttcccagag
     7381 taaaagtagc cattggatga ccacttggct acaacacgga gccctacaaa gctatttcct
     7441 ggagaggagg catctaagcc atcagaaggg ccagcagcag cctggaaagg aatttctgga
     7501 gagtcactac gacgcaaagc accagcaccc acatctgttc gtctggtgga gtctggcact
     7561 cggggcacga cacggctgaa ggatttatca tctggtgaca agttaggtga agcggccgct
     7621 gcgcccaagg ggccaggcac agctgtttct ctggttccag tggtccgaga aggtgggcgg
     7681 ccccttcgcc cacgcccacg aggcgtgact ggagccttcc ctccctgtct gatgccaagg
     7741 cctgcatcac catcctcctc acacactggc gtccctgtct gactgacccc ttttctagga
     7801 ctcagttttc ctgggcctcc tcgggagctg ggtaaggcaa aatctgcggg ttctgtcccg
     7861 ctggttttcc ctctgagtgg tccggctcct ttccctgagc ttccactgct gtgcatagct
     7921 gagagacttg tcccacttga tgtgcggtgt aagctggatg ccttggagga gaaggagctg
     7981 atatccccca ggtcacctga ggagccccca gtctgtgaag gggaaacttc agtttcacac
     8041 tcctgacact ctacaattgg ctcttcagtc tcctcagtta cttttctttc tacttctgtt
     8101 ccatccacat aatacacatc tgtaatgaca cgagtgacaa gtgtgcgtac ttcccggatt
     8161 gttctcatgt gacgatgtaa gacatggcca tgtggaggct gaggcatatc aaactcttct
     8221 tctccctggc tatggagcga ctctgtatca tccccaggag cactgactgg tttctcacca
     8281 ctccccctct cagtctgaac tgtggcatct tgctttccaa agttctggtc cactgtactg
     8341 gtcccctgct cacacacatt ctttatagtc tgggttgctg ctgaaacagt ttctgggacc
     8401 ctcaaggaac actccatggt ttggattcct atgttatttt ggctgggcct ttcaatcaag
     8461 gccttactag gattttcctt attagtactc cgtccttctt tctggtcttc tagcacatct
     8521 gtcaccattg cctctccttg aggactggat ggcccttgta tgaccatctt ctgggaagca
     8581 ggagaaacag ggtccttgac aggactaatg ggcttcatag gctgttgact ctgcctgatt
     8641 gtatggtcac cctccaattt ttctttctcc tcttctcctt gagaacttgg aaagtggagc
     8701 aagttccccc tgatgggtgt ggtgggggga tcctcacttc gagcctcatt ctcttgcctg
     8761 gcttcacaga tacagctcaa cacagacatg gtcttctggg gactggcaac agactcagca
     8821 acagcagtag atccattttt tctttcacca gttgcaggct tttccaggtt gaactgcaaa
     8881 gactcttcac tcgcctcagt ctcaggacta accagtttca ttcttagaca taatttatca
     8941 tccacgtctg gggcagcccc agaatcccct tttccactcc caagtatggg atcatgggtc
     9001 tccaccatgc tttcagacat gacatcactg gttgctatgg tgctttctgg atagttgcta
     9061 ataccaatag gagtactgtg tctcttgggc tccaatttta ggtgcccaat aagaggtggg
     9121 gttgcaccag tcaatggtgg gatgatatca ccttctttag gcaaagtgaa atgaaatggg
     9181 gtttcactag aactttcaca gaagctttgt gaggcatggg cagagggctt ccccagcttc
     9241 tgggcctctg catctgagct tagctgcttt gcattagcca tttttgagtc ttctgttaat
     9301 gaattacttg ttttctcctg agtttggggc tgctgcaact cctggtcaag tcttaaagga
     9361 tcatctgctc tcactaaagg taaggaaggc tgtgaagaat cttgctctac aggttctgtt
     9421 tctgcagtcc ctgatttcag atctccttca tattctacac tcttttcttc cttggtgtct
     9481 aatctattct cagcacatgg ttctatttct ggagcaatat cctcccatga ctgggaatct
     9541 gagcacttct ccactccctc caaaggttca caagaaacat caactctggg agatggctct
     9601 ttcacatcta caatgacaac actggagtcc tctgaagtag cttcttccca agcttcctgt
     9661 tccttatgtt ccaattcttg gtcaagtatt gccaactttt gaggggaatc aatactaatc
     9721 acactggttt caacttccat agcttctgag cattcttttt ttggcatttc cttttgaaga
     9781 cacaaccctt gggactgagt ttcagtcaga gaaaggtgca acggaacact ctccatattt
     9841 tcttctttga gttcctctcc ttgactttca caaggtgtct cagggatttc ttccacctca
     9901 gaccctgaag acccctcctc tggatggtgt tctttaattt ccatagcttc ctcctgatct
     9961 aacacactag aaagtgcctc agatcgagta gctggtgacg gaactgcctg actccccgaa
    10021 tcacaagtga gatcaataca aacatcttct gctaccgttt cttctctgcc cttgcaacca
    10081 gtggctaaaa tactaatgtc atccctggtg tctgtatcat ctccctttgt tttgtcatca
    10141 ttctgactca gttgtacttc accatcctgt gctggattca tcaggatact atcattttca
    10201 gcaggaacaa atttagaatt gagaactgga gacatgggtt ccgtatcctc aatctgtgtg
    10261 ttttctccat cttcatcaat tctgtgagaa ctcaagctct ccatctttgg ggactgacta
    10321 tattcacttg tagaaagcat caacttgcaa gaatcccctg tcaaagatag cccaagatcc
    10381 tcaggggaat tctttggttc aatctctgaa gttttagaac actcaactgt caaagatgaa
    10441 ctatgcatat ctccatcttt cttcccatca tttgattgtt cttccagact tggggaagga
    10501 ataaaaatgt catgagaaaa ctgaggctgg gatgggatag gaagtgaccc aggagggaag
    10561 actggtgtgc tctgagatat tggtgttgag gcttgtggtg atacagtgga ctcaggcagg
    10621 agaggggggt tttctaactc cactggttca ccactttgaa gtttcttctg aaaaggctct
    10681 cctccttctt cagataacac tgacgtgtcc attggcttat cttgtctccc ttcttgctct
    10741 gtgggactgc taggaacgat aaaaggagta gatcggaaag catcaggaga aggagcaaca
    10801 agatctgaag aattcgtgga aagactgtcc tgaacaaggg acctctgacc agagagctgc
    10861 aggagatgca gagtggtggc aggagtggaa gccaaagaac acccaccttc ctcccttgaa
    10921 ggagtagagc aaccatcaga agatactgtt ttattgctct ggtcaaacaa gtcttcctga
    10981 gttgacaaaa cctcaggctc tggtgacttc tgaatctgca gtccactttc cataagttct
    11041 tgtgcagaca actgttcttt tgcttccata gcagcaacag atgctttggg gctaaaaggc
    11101 atgtcctctg accttgcagg tggtggattt tgctctttta caacatgtac atccttactg
    11161 ggctgtgctg tcacagggat gtccttgctg gactgttctg ctatggggat atcttcgttg
    11221 gactgttctt catgcttaat tgcagtatta gcatccacat cagacagcct ggtataacca
    11281 gagttggtgg ttactgattg tagctgctct ttgtccactt catatggcac agtattttcc
    11341 tcaacatcct ggctctggga gagttccaga accccaaaac ccaactgtga tgaggcagta
    11401 tcttcagcac caagggaatg tgtagtattg cctgaagtat cttcttcctt ctctttctcc
    11461 ttctgttcca ac
//
