LOCUS       sequence-473454-       10400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A piggybac-based cloning vector containing TRE3G promoter followed 
            by cloning site and CAG promoter-driven nuclear-localized 
            Clover-P2A-rtTA-IRES-BSD..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10400)
  AUTHORS   Okubo C, Nakamura M, Sato M, Shichino Y, Mito M, Takashima Y, 
            Iwasaki S, Takahashi K
  TITLE     EIF3D safeguards the homeostasis of key signaling pathways in human 
            primed pluripotency.
  JOURNAL   Sci Adv. 2025 Apr 11;11(15):eadq5484. doi: 10.1126/sciadv.adq5484. 
            Epub 2025 Apr 9.
  PUBMED    40203091
REFERENCE   2  (bases 1 to 10400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-04-11"; volume: "11(15):eadq5484. doi"; pages: " 
            10.1126/sciadv.adq5484. Epub 2025 Apr 9"
COMMENT     Sequence Label: PB-TRE3G-CCRB
FEATURES             Location/Qualifiers
     source          1..10400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1418..1436
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1454..1472
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1490..1508
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1526..1544
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1562..1580
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1598..1616
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1634..1652
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1697..1721
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    1834..2422
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1887..1907)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2305..2316)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2455..2576
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2492..2511)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2546..2565
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     enhancer        2655..2958
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          3237..4253
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4177..4199
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4261..4280
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      4320..4329
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4332..4352
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4356..4376
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4380..4400
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(4416..4418,4419..4421,4422..5132)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(4461..4482)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     5069..5090
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5142..5198
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5199..5945
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    join(5962..6536,6537..6539,6540..6548)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6129..6146)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6356..6375
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6549..6947
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(6985..7002)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6991..7215
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   7854..7888
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8379..8395)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8379..8395)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8392..8414)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8403..8419
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8427..8433,8434..8451,8452..8457))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8472..8493
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8610..8627)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8781..9369)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8861..8880)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9540..10331,10332..10400))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10163..10182
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggcccgat gaggcccttt cgtcttcact
     1381 cgaggatcgc tgatatctcc accccacagt ggtttactcc ctatcagtga tagagaacgt
     1441 atgaagagtt tactccctat cagtgataga gaacgtatgc agactttact ccctatcagt
     1501 gatagagaac gtataaggag tttactccct atcagtgata gagaacgtat gaccagttta
     1561 ctccctatca gtgatagaga acgtatctac agtttactcc ctatcagtga tagagaacgt
     1621 atatccagtt tactccctat cagtgataga gaacgtataa gctttaggcg tgtacggtgg
     1681 gcgcctataa aagcagagct cgtttagtga accgtcagat cgcctggagc aattccacaa
     1741 cacttttgtc ttataccaac tttccgtacc acttcctacc ctcgtaaact taaggttaat
     1801 taagatatcg tatacgatcc attaggcgcg gccaatcaac ctctggatta caaaatttgt
     1861 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     1921 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     1981 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     2041 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     2101 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     2161 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     2221 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     2281 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     2341 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     2401 tccctttggg ccgcctcccc gcaccggtaa aggccgcaat cgatcgccca ccccaacttg
     2461 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     2521 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     2581 gtctgtatac gcactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     2641 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     2701 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     2761 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     2821 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     2881 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     2941 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc
     3001 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg
     3061 ggggcggggg gggggggggg cgcgcgccrg gsggggsggg gsggggsggg ggggggggsg
     3121 gggsgrggcg grgaggkgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
     3181 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
     3241 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
     3301 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
     3361 gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc
     3421 ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt
     3481 gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg
     3541 ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg
     3601 gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     3661 tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc
     3721 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg
     3781 cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc
     3841 cgcctcgggc cggggrgggc tcgggggrgg ggcgcggcgg cccccggagc gccggcggct
     3901 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     3961 gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct
     4021 ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc
     4081 ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg
     4141 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     4201 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
     4261 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattccct taaggttacg
     4321 ccaccatggt gccaaagaag aagaggaaag tggaccccaa gaaaaaaaga aaggttgatc
     4381 caaaaaagaa aagaaaagta cacgtgtcaa cgcgtatggt gagcaagggc gaggagctgt
     4441 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     4501 gcgtccgcgg cgagggcgag ggcgatgcca ccaacggcaa gctgaccctg aagttcatct
     4561 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcg
     4621 tggcctgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     4681 tgcccgaagg ctacgtccag gagcgcacca tctctttcaa ggacgacggt acctacaaga
     4741 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     4801 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac ttcaacagcc
     4861 acaacgtcta tatcacggcc gacaagcaga agaacggcat caaggctaac ttcaagatcc
     4921 gccacaacgt tgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     4981 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagccatcag tccgccctga
     5041 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     5101 ggattacaca tggcatggac gagctgtaca agggatctgg cgccacgaac ttctctctgt
     5161 taaaacaagc aggagacgtg gaagaaaatc ccggtccgat gagccggctg gacaagagca
     5221 aagtgatcaa cagcgccctg gaactgctga acggcgtggg catcgagggc ctgaccaccc
     5281 ggaagctggc ccagaaactg ggcgtggaac agcccaccct gtactggcac gtgaagaaca
     5341 agcgggccct gctggacgcc ctgcccatcg agatgctgga ccggcaccac acccacagct
     5401 gccctctgga aggcgagagc tggcaggact tcctgcggaa caacgccaag agctacagat
     5461 gcgccctgct gagccaccgg gacggcgcca aagtgcacct gggcaccaga cccaccgaga
     5521 agcagtacga gacactggaa aaccagctgg ccttcctgtg ccagcagggc ttcagcctgg
     5581 aaaacgccct gtacgccctg agcgccgtgg gccacttcac cctgggctgc gtgctggaag
     5641 aacaggaaca ccaggtcgcc aaagaggaaa gagagacacc caccaccgac agcatgcccc
     5701 ccctgctgaa gcaggccatc gagctgttcg acagacaggg cgccgagccc gccttcctgt
     5761 tcggcctgga actgatcatc tgcggcctcg agaagcagct gaagtgcgag agcggcggac
     5821 ccaccgacgc cctggacgac ttcgacctgg acatgctgcc cgccgatgcc ctggatgatt
     5881 ttgatctgga tatgctccct gctgacgctc tcgatgactt cgatctcgat atgctgccag
     5941 gctaagttag atctaattcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag
     6001 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     6061 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     6121 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     6181 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     6241 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     6301 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     6361 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     6421 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     6481 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     6541 ccacaaccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg
     6601 ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta
     6661 gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag
     6721 aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg
     6781 cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc
     6841 tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag
     6901 ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaagtc gagcctctag
     6961 agttatctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     7021 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     7081 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     7141 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     7201 cggtgggctc tatggcttct gaggcggaag tcaaccaggt tgcagggttt cctgtcctca
     7261 caggaacgaa gtccctaaag aaacagtggc agccaggttt agccccggaa ttgactggat
     7321 tcctttttta gggcccattg gtatggcttt ttccccgtat ccccccaggt gtctgcaggc
     7381 tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg
     7441 ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga gcgccggacc ggagcggagc
     7501 cccgggcggc tcgctgctgc cccctagcgg gggagggacg taattacatc cctgggggct
     7561 ttgggggggg gctgtccctg atatctataa caagaaaata tatatataat aagttatcac
     7621 gtaagtagaa catgaaataa caatataatt atcgtatgag ttaaatctta aaagtcacgt
     7681 aaaagataat catgcgtcat tttgactcac gcggtcgtta tagttcaaaa tcagtgacac
     7741 ttaccgcatt gacaagcacg cctcacggga gctccaagcg gcgactgaga tgtcctaaat
     7801 gcacagcgac ggattcgcgc tatttagaaa gagagagcaa tatttcaaga atgcatgcgt
     7861 caattttacg cagactatct ttctagggtt aatctagctg catcaggatc atatcgtcgg
     7921 gtcttttttc cggctcagtc atcgcccaag ctggcgctat ctgggcatcg gggaggaaga
     7981 agcccgtgcc ttttcccgcg aggttgaagc ggcatggaaa gagtttgccg aggatgactg
     8041 ctgctgcatt gacgttgagc gaaaacgcac gtttaccatg atgattcggg aaggtgtggc
     8101 catgcacgcc tttaacggtg aactgttcgt tcaggccacc tgggatacca gttcgtcgcg
     8161 gcttttccgg acacagttcc ggatggtcag cccgaagcgc atcagcaacc cgaacaatac
     8221 cggcgacagc cggaactgcc gtgccggtgt gcagattaat gacagcggtg cggcgctggg
     8281 atattacgtc agcgaggacg ggtatcctgg ctggatgccg cagaaatgga catggatacc
     8341 ccgtgagtta cccggcgggc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     8401 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     8461 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     8521 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     8581 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     8641 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8701 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8761 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8821 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8881 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8941 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     9001 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9061 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9121 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9181 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     9241 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9301 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9361 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     9421 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     9481 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     9541 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9601 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9661 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9721 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9781 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9841 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9901 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9961 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    10021 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    10081 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    10141 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    10201 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    10261 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    10321 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    10381 cggaaatgtt gaatactcat
//
