LOCUS       sequence-47348-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Angus SP, Nevins JR
  TITLE     A role for Mediator complex subunit MED13L in Rb/E2F-induced growth 
            arrest.
  JOURNAL   Oncogene. 2012 Jan 16. doi: 10.1038/onc.2011.622.
  PUBMED    22249253
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2012 Jan 16. doi: 10.1038/onc.2011.622."
COMMENT     Sequence Label: pAdTrackCMV-PSM-Rb
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(61..264)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(90..110)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        265..568
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    654..775
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(665..684)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     719..738
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tcgcccttgc tcccatggtg gcgaccggta gcgctagcgg atctgacggt tcactaaacc
       61 agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc
      121 ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt
      181 gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt
      241 gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg
      301 ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc
      361 gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg
      421 ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac
      481 tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg
      541 cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga
      601 ccccgtaatt gattactatt aatcactagc agatcgtcga taaacctccg cgttaagata
      661 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
      721 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
      781 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggaag tttttaaaca
      841 gtaaac
//
