LOCUS       sequence-473508-       16733 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LentiCRISPR v2 was modified into an all-in-one dox inducible system.
            The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter 
            drives constitutive sgRNA expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16733)
  AUTHORS   Barger CJ, Branick C, Chee L, Karpf AR
  TITLE     Pan-Cancer Analyses Reveal Genomic Features of FOXM1 Overexpression 
            in Cancer.
  JOURNAL   Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 
            10.3390/cancers11020251.
  PUBMED    30795624
REFERENCE   2  (bases 1 to 16733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancers 
            (Basel)."; date: "2019-02-21"; volume: "11(2). pii: cancers11020251.
            doi"; pages: " 10.3390/cancers11020251"
COMMENT     Sequence Label: TLCV2
FEATURES             Location/Qualifiers
     source          1..16733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(53..55,56..769)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(53..55,56..766)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(356..375)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     703..724
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        780..991
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             1013..1609
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1013..1032)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1509..1529
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1619..1675
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1676..2293,2294..2299,2300..2335,2336..2338,2339..2374,
                     2375..2377,2378..2413,2414..2422)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    2443..3031
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2496..2516)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2914..2925)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3103..3336
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(3362..3379)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3368..3592
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3638..4066
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3725..3744)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3935..3956
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4075..4095)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4080..4409
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4260..4395
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4322..4341
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4457..4504
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4523..4897
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5027..5148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5064..5083)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5118..5137
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5197..5213)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5197..5213)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5210..5232)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5221..5237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5245..5251,5252..5269,5270..5275))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5290..5311
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5428..5445)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5599..6187)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5679..5698)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6358..7149,7150..7218))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6981..7000
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7219..7323)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7398..7417)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7589..7968
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7970..8168
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8186..8366
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8413..8538
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9031..9264
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9449..9493
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9791..9908
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9959..10207
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     9959..9979
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     10130..10149
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        12089..12164
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        12201..12515
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    12209..12227
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12245..12263
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12280..12298
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12316..12334
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12352..12370
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12387..12405
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12423..12441
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     12488..12512
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      12541..12550
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             12547..16650
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             12547..16650
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             16651..16698
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             16699..16722
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(16732..16733,1..52)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gggcagagga agtctgctaa catgcggtga cgtcgaggag aatcctggcc cagtgagcaa
       61 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      121 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      181 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      241 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
      301 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      361 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      421 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      481 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
      541 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
      601 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
      661 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
      721 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag ctagcatccg
      781 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgatc
      841 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
      901 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
      961 ttttcgcaac gggtttgccg ccagaacaca gactgcgatc gcaatgtaca gtatgaccga
     1021 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     1081 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     1141 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     1201 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     1261 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     1321 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     1381 gttcctggcc accgtcggag tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     1441 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     1501 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     1561 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgccg gttccggcgc
     1621 aacaaacttc tctctgctga aacaagccgg agatgtcgaa gagaatcctg gaccgatgtc
     1681 tagactggac aagagcaaag tcataaacgg agctctggaa ttactcaatg gtgtcggtat
     1741 cgaaggcctg acgacaagga aactcgctca aaagctggga gttgagcagc ctaccctgta
     1801 ctggcacgtg aagaacaagc gggccctgct cgatgccctg ccaatcgaga tgctggacag
     1861 gcatcatacc cacttctgcc ccctggaagg cgagtcatgg caagactttc tgcggaacaa
     1921 cgccaagtca taccgctgtg ctctcctctc acatcgcgac ggggctaaag tgcatctcgg
     1981 cacccgccca acagagaaac agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca
     2041 gcaaggcttc tccctggaga acgcactgta cgctctgtcc gccgtgggcc actttacact
     2101 gggctgcgta ttggaggaac aggagcatca agtagcaaaa gaggaaagag agacacctac
     2161 caccgattct atgcccccac ttctgagaca agcaattgag ctgttcgacc ggcagggagc
     2221 cgaacctgcc ttccttttcg gcctggaact aatcatatgt ggcctggaga aacagctaaa
     2281 gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt gacttagaca tgctcccagc
     2341 cgatgccctt gacgactttg accttgatat gctgcctgct gacgctcttg acgattttga
     2401 ccttgacatg ctccccgggt aactcgagcg cgttaagtcg acaatcaacc tctggattac
     2461 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     2521 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     2581 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     2641 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     2701 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     2761 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     2821 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     2881 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     2941 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     3001 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     3061 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     3121 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     3181 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     3241 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     3301 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
     3361 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     3421 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     3481 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     3541 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     3601 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     3661 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     3721 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     3781 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     3841 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     3901 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     3961 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     4021 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     4081 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4141 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4201 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     4261 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     4321 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     4381 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     4441 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     4501 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     4561 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     4621 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     4681 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     4741 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     4801 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     4861 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     4921 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     4981 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     5041 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     5101 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     5161 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     5221 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     5281 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     5341 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     5401 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     5461 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     5521 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     5581 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     5641 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     5701 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     5761 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     5821 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     5881 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     5941 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6001 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     6061 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6121 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     6181 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     6241 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     6301 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     6361 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     6421 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     6481 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     6541 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     6601 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     6661 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     6721 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     6781 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     6841 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     6901 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     6961 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     7021 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     7081 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     7141 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     7201 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     7261 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     7321 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
     7381 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     7441 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     7501 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     7561 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
     7621 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     7681 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     7741 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     7801 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     7861 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     7921 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     7981 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     8041 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     8101 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     8161 ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     8221 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     8281 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     8341 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     8401 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     8461 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     8521 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     8581 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     8641 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     8701 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     8761 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     8821 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     8881 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     8941 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     9001 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     9061 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     9121 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     9181 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     9241 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     9301 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     9361 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     9421 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     9481 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     9541 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     9601 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     9661 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     9721 cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat
     9781 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     9841 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     9901 aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt aaggtaccga
     9961 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat
    10021 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
    10081 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
    10141 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
    10201 aaacaccgga gacggttgta aatgagcaca caaaatacac atgctaaaat attatattct
    10261 atgaccttta taaaatcaac caaaatcttc tttttaataa ctttagtatc aataattaga
    10321 atttttatgt tcctttttgc aaacttttaa taaaaatgag caaaataaaa aaacgctagt
    10381 tttagtaact cgcgttgttt tcttcacctt taataatagc tactccacca cttgttccta
    10441 agcggtcagc tcctgcttca atcatttttt gagcatcttc aaatgttcta actccaccag
    10501 ctgctttaac taaagcattg tctttaacaa ctgacttcat tagtttaaca tcttcaaatg
    10561 ttgcacctga ttttgaaaat cctgttgatg ttttaacaaa ttctaatcca gcttcaacag
    10621 ctatttcaca agctttcatg atttcttctt ttgttaataa acaattttcc ataatacatt
    10681 taacaacatg tgatccagct gcttttttta cagctttcat gtcttctaaa actaattcat
    10741 aatttttgtc ttttaatgca ccaatattta ataccatatc aatttctgtt gcaccatctt
    10801 taattgcttc agaaacttcg aatgcttttg tagctgttgt gcatgcacct agaggaaaac
    10861 ctacaacatt tgttattcct acatttgtgc cttttaataa ttctttacaa tagcttgttc
    10921 aatatgaatt aacacaaact gttgcaaaat caaattcaat tgcttcatca cataattgtt
    10981 taatttcagc tttcgtagca tcttgtttta ataatgtgtg atctatatat ttgtttagtt
    11041 tcattttttc tcctatatat tcatttttaa ttttaattct ttaataattt cgtctacttt
    11101 aactttagcg ttttgaacag attcaccaac acctataaaa taaattttta gtttaggttc
    11161 agttccactt gggcgaacag caaatcatga cttatcttct aaataaaatt ttagtaagtc
    11221 ttgtcctggc atattataca ttccatcgat gtagtcttca acattaacaa ctttaagtcc
    11281 agcaatttga gttaagggtg ttgctctcaa tgatttcatt aatggttcaa tttttaattt
    11341 cttttcttct ggtttaaaat tcaagtttaa agtgaaagtg taatatgcac ccatttcttt
    11401 aaataaatct tctaaatagt ctactaatgt tttattttgt tttttataaa atcaagcagc
    11461 ctctgctatt aatatagaag cttgtattcc atctttatct ctagctgagt catcaattac
    11521 atatccataa ctttcttcat aagcaaaaac aaaatttaat ccgttatctt cttctttagc
    11581 aatttctcta cccattcatt taaatccagt taaagttttt acaatattaa ctccatattt
    11641 ttcatgagcg attctatcac ccaaatcact tgttacaaaa cttgaatata gagccggatt
    11701 ttttggaatg ctatttaagc gttttagatt tgataatttt caatcaatta aaattggtcc
    11761 tgtttgattt ccatctaatc ttacaaaatg accatcatgt tttattgcca ttccaaatct
    11821 gtcagcatct gggtcattca taataataat atctgcatca tgtttaatac catattcaag
    11881 cggtattttt catgcaggat caaattctgg atttggattt acaacatttt taaatgtttc
    11941 atcttcaaat gcatgctctt caacctcaat aacgttatat cctgattcac gtaatatttt
    12001 tggggtaaat ttagttcctg ttccattaac tgcgctaaaa ataattttta aatctttttt
    12061 agcttcttgc tcttttttgt acgtctctgt tttagagcta gaaatagcaa gttaaaataa
    12121 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt gaattcactt
    12181 tggccgcgaa tcgatatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt
    12241 ttactcccta tcagtgatag agaacgatgt cgagtttact ccctatcagt gatagagaac
    12301 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca
    12361 gtgatagaga acgtatgtcg agtttatccc tatcagtgat agagaacgta tgtcgagttt
    12421 actccctatc agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata
    12481 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag aataccggtt ctagagcgct
    12541 gccaccatgg acaagaagta cagcatcggc ctggacatcg gcaccaactc tgtgggctgg
    12601 gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc
    12661 gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca
    12721 gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg
    12781 atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc
    12841 cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc
    12901 ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg
    12961 agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg
    13021 gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac
    13081 agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa
    13141 aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag
    13201 agcagacggc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc
    13261 ggaaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg
    13321 gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct ggacaacctg
    13381 ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac
    13441 gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc
    13501 gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc
    13561 gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc
    13621 tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt catcaagccc
    13681 atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg
    13741 ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag
    13801 ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa
    13861 aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga
    13921 aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc
    13981 gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat gaccaacttc
    14041 gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc
    14101 accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc
    14161 ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac caaccggaaa
    14221 gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg
    14281 gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg
    14341 aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat
    14401 atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc
    14461 tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc
    14521 tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca
    14581 atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac
    14641 gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat
    14701 agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa gggcatcctg
    14761 cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac
    14821 atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc
    14881 gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa
    14941 caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta cctgcagaat
    15001 gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg
    15061 gaccatatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa ggtgctgacc
    15121 agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag
    15181 atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag aaagttcgac
    15241 aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg cttcatcaag
    15301 agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg
    15361 atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt gatcaccctg
    15421 aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc
    15481 aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc
    15541 aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg
    15601 cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc
    15661 tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg
    15721 aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg
    15781 gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc
    15841 gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag
    15901 ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc
    15961 gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag
    16021 agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc
    16081 atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg
    16141 cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgccggc
    16201 gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg
    16261 gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt
    16321 gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag
    16381 agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg
    16441 gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg
    16501 ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc
    16561 accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca
    16621 cggatcgacc tgtctcagct gggaggcgac aagcgacctg ccgccacaaa gaaggctgga
    16681 caggctaaga agaagaaaga ttacaaagac gatgacgata agggatccgg aga
//
