LOCUS       sequence-473523-        9308 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression, SV40-puro; PGK-gateway-V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9308)
  TITLE     Root Lab pLEX and pLIX Gateway backbones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLEX_306
FEATURES             Location/Qualifiers
     source          1..9308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    640..765
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             812..992
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(993..1219)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1247..1267)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1247..1265)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1286..1302)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1286..1302)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1299..1321)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1310..1326
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1334..1340,1341..1358,1359..1364))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1379..1400
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1517..1534)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1688..2276)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1768..1787)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2447..3238,3239..3307))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3070..3089
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3308..3412)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3438..3893)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3575..3596)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3787..3806
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4020..4042
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4034..4051
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4035..4051
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4061..4080
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4061..4079
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4100..4235
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4275..4396
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4286..4305)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4340..4359
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4474..4707
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4779..5367
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4885..4896
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5294..5314
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5418..5459)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    5467..5591
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(5632..5937)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(5734..5753)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(6279..6938)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6853..6872
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(6992..6998,6999..7016,7017..7022))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(7047..7171)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(7192..7692)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7255..7273)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    7750..7867
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7910..8512)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(7996..8016)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8493..8512
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(8544..8740)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(8552..8762)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8558..8693
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(8993..9037)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(9222..9308,1..147)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct gctgcactat accagacaat
       61 aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct
      121 cccaagaacc caaggaacaa agctcctatt cccactgctc ttttttctct ctgcaccact
      181 cttctctttg ccttggtggg tgctactcct aatggttcaa tttttactac tttatattta
      241 tataattcac ttctccaatt gtccctcata tctcctcctc caggtctgaa gatcagcggc
      301 cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc
      361 cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta ctgtattata
      421 taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg
      481 tctacagcct tctgatgttt ctaacaggcc aggattaact gcgaatcgtt ctagctccct
      541 gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc ttaaccgaat
      601 tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc acccatctct
      661 ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc
      721 tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct ctggtttccc
      781 tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag
      841 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
      901 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
      961 caggctcaga tctggtctaa ccagagagac ccgtttatgt atcgagctag gcacttaaat
     1021 acaatatctc tgcaatgcgg caattcagtg gttcgtccaa tccatgtcag acccgtctgt
     1081 tgccttccta ataaggcacg atcgtaccac cttacttcca ccaatcggca tgcacggtgc
     1141 tttttctctc cttgtaaggc atgttgctaa ctcatcgtta ccatgttgca agactacaag
     1201 agtattgcat aagactacat taagcttgca gctccagctt ttgttccctt tagtgagggt
     1261 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     1321 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     1381 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     1441 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     1501 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     1561 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     1621 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1681 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1741 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1801 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1861 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1921 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1981 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     2041 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     2101 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     2161 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2221 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     2281 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2341 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     2401 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     2461 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     2521 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     2581 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     2641 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     2701 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     2761 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     2821 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     2881 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     2941 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     3001 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     3061 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     3121 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     3181 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3241 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3301 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3361 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3421 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     3481 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     3541 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     3601 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     3661 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     3721 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     3781 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     3841 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
     3901 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     3961 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     4021 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
     4081 cgaattgggt acgtccctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     4141 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     4201 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggatagct
     4261 agagccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     4321 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     4381 ctgcaataaa caagttcctc tcactctctg atattcattt ctttgcaagt tataaatact
     4441 gaataataag atgacatgaa ctactatacg tactgctaga gattttccac actgactaaa
     4501 agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa
     4561 gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct
     4621 cccaggctca gatctggtct aaccagagag acccagtaca agcaaaaagc agatcttgtc
     4681 ttcgttggga gtgaattagc ccttccagtc cccccttttc ttttaaaaag tggctaagat
     4741 ctacagctgc cttgtaagtc attggtctta aagtcgacgc ggggaggcgg cccaaaggga
     4801 gatccgactc gtctgagggc gaaggcgaag acgcggaaga ggccgcagag ccggcagcag
     4861 gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg acgtagcaga aggacgtccc
     4921 gcgcagaatc caggtggcaa cacaggcgag cagccaagga aaggacgatg atttccccga
     4981 caacaccacg gaattgtcag tgcccaacag ccgagcccct gtccagcagc gggcaaggca
     5041 ggcggcgatg agttccgccg tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag
     5101 ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca cagtgcacac
     5161 cacgccacgt tgcctgacaa cgggccacaa ctcctcataa agagacagca accaggattt
     5221 atacaaggag gagaaaatga aagccatacg ggaagcaata gcatgataca aaggcattaa
     5281 agcagcgtat ccacatagcg taaaaggagc aacatagtta agaataccag tcaatctttc
     5341 acaaattttg taatccagag gttgattgtc gacttaacgc gttgattgtc gacttaaccg
     5401 gtactagttc attactacgt agaatcgaga ccgaggagag ggttagggat aggcttaccg
     5461 atatcaacca ctttgtacaa gaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
     5521 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
     5581 cagtcactat ggtcgacctg cagactggct gtgtataagg gagcctgaca tttatattcc
     5641 ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga gatcagccac
     5701 ttcttccccg ataacggaga ccggcacact ggccatatcg gtggtcatca tgcgccagct
     5761 ttcatccccg atatgcacca ccgggtaaag ttcacgggag actttatctg acagcagacg
     5821 tgcactggcc agggggatca ccatccgtcg cccgggcgtg tcaataatat cactctgtac
     5881 atccacaaac agacgataac ggctctctct tttataggtg taaaccttaa actgcatttc
     5941 accagcccct gttctcgtca gcaaaagagc cgttcatttc aataaaccgg gcgacctcag
     6001 ccatcccttc ctgattttcc gctttccagc gttcggcacg cagacgacgg gcttcattct
     6061 gcatggttgt gcttaccaga ccggagatat tgacatcata tatgccttga gcaactgata
     6121 gctgtcgctg tcaactgtca ctgtaatacg ctgcttcata gcatacctct ttttgacata
     6181 cttcgggtat acatatcagt atatattctt ataccgcaaa aatcagcgcg caaatacgca
     6241 tactgttatc tggcttttag taagccggat ccagatcttt acgccccgcc ctgccactca
     6301 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     6361 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     6421 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     6481 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa
     6541 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     6601 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     6661 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg
     6721 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     6781 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     6841 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     6901 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
     6961 aatctcgacg gatcctaact caaaatccac acattatacg agccggaagc ataaagtgta
     7021 aagcctgggg tgcctaatgc ggccgccata gtgactggat atgttgtgtt ttacagtatt
     7081 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
     7141 tttctcgttc agcttttttg tacaaacttg ttgatcaatc gatgctagcc cctggggaga
     7201 gaggtcggtg attcggtcaa cgagggagcc gactgccgac gtgcgctccg gaggcttgca
     7261 gaatgcggaa caccgcgcgg gcaggaacag ggcccacact accgccccac accccgcctc
     7321 ccgcaccgcc ccttcccggc cgctgctctc ggcgcgccct gctgagcagc cgctattggc
     7381 cacagcccat cgcggtcggc gcgctgccat tgctccctgg cgctgtccgt ctgcgagggt
     7441 actagtgaga cgtgcggctt ccgtttgtca cgtccggcac gccgcgaacc gcaaggaacc
     7501 ttcccgactt aggggcggag caggaagcgt cgccgggggg cccacaaggg tagcggcgaa
     7561 gatccgggtg acgctgcgaa cggacgtgaa gaatgtgcga gacccagggt cggcgccgct
     7621 gcgtttcccg gaaccacgcc cagagcagcc gcgtccctgc gcaaacccag ggctgccttg
     7681 gaaaaggcgc aacctgcagg cctcgagccg gcgccaaagt ggatctctgc tgtccctgta
     7741 ataaacccga aaattttgaa tttttgtaat ttgtttttgt aattctttag tttgtatgtc
     7801 tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc ccccttttct
     7861 tttaaaattg tggatgaata ctgccatttg tctcaagatc tagaattcgt taactggcac
     7921 cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg tcggcggtga
     7981 cggtgaagcc gagccgctcg tagaagggga ggttgcgggg cgcggaggtc tccaggaagg
     8041 cgggcacccc ggcgcgctcg gccgcctcca ctccggggag cacgacggcg ctgcccagac
     8101 ccttgccctg gtggtcgggc gagacgccga cggtggccag gaaccacgcg ggctccttgg
     8161 gccggtgcgg cgccaggagg ccttccatct gttgctgcgc ggccagccgg gaaccgctca
     8221 actcggccat gcgcgggccg atctcggcga acaccgcccc cgcttcgacg ctctccggcg
     8281 tggtccagac cgccacggcg gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc
     8341 cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg cggtccggat
     8401 cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc
     8461 tggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc atggtaagct
     8521 ccggtggtac ctctagaaag ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac
     8581 tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt
     8641 agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga
     8701 gttaggggcg ggactatggt tgctgactaa ttgagacgca tgctttgcat acttctgcct
     8761 gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg caatgcatgg
     8821 gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc tgcctaactc
     8881 tattcactat agaaagtaca gcaaaaacta ttcttaaacc taccaagcct cctactatca
     8941 ttatgaataa ttttatatac cacagccaat ttgttatgtt aaaccaattc cacaaacttg
     9001 cccatttatc taattccaat aattcttgtt cattcttttc ttgctggttt tgcgattctt
     9061 caattaagga gtgtattaag cttgtgtaat tgttaatttc tctgtcccac tccatccagg
     9121 tcgtgtgatt ccaaatctgt tccagagatt tattactcca actagcattc caaggcacag
     9181 cagtggtgca aatgagtttt ccagagcaac cccaaatccc caggagctgt tgatccttta
     9241 ggtatctttc cacagccagg attcttgcct ggagctgctt gatgccccag actgtgagtt
     9301 gcaacaga
//
