LOCUS       sequence-473528-       15050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid of PEmax prime editor with P2A-BSD 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15050)
  AUTHORS   Yu G, Kim HK, Park J, Kwak H, Cheong Y, Kim D, Kim J, Kim J, Kim HH
  TITLE     Prediction of efficiencies for diverse prime editing systems in 
            multiple cell types.
  JOURNAL   Cell. 2023 Apr 21:S0092-8674(23)00331-8. doi: 
            10.1016/j.cell.2023.03.034.
  PUBMED    37119812
REFERENCE   2  (bases 1 to 15050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2023 
            Apr 21:S0092-8674(23)00331-8. doi: 10.1016/j.cell.2023.03.034."
COMMENT     Sequence Label: pLenti-PE2max-BSD
FEATURES             Location/Qualifiers
     source          1..15050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(407..427)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(483..694)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    787..904
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1202..1246)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1431..1664
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2157..2282
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2329..2509
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2527..2725)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2552..2572)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2727..3106
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     3278..3297
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3372..3476
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3477..3545,3546..4337)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3695..3714)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4508..5096
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4997..5016
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5250..5267
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5384..5405
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5420..5425,5426..5443,5444..5450)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5458..5474
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5463..5485
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5482..5498
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5482..5498
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    5547..5668
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5558..5577)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5612..5631
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5798..6172)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(6191..6238)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      6300..6434
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6354..6373)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     6579..6599
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(6608..7036)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6718..6739)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6930..6949
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    7082..7306
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7295..7312
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             7338..7518
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     8023..8039
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     8024..8040
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    8043..8631
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             8149..8160
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8558..8578
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8656..9054)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(9055..9111)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(9121..9147)
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             complement(9157..9177)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(11278..11298)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(11356..15050,1..406))
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 ggtagatggt ggggtacttc tcgtggtagg ccacctcgtc cacgatgttg ccgaagatgg
       61 ggtgccgctc gtgcttctta tcctcttcca ccaggaagga ctcttccagt ctgtggaaga
      121 agctgtcgtc caccttggcc atctcgttgc tgaagatctc ttgcagatag cagatccggt
      181 tcttccgtct ggtgtatctt cttctggcgg ttctcttcag ccgggtggcc tcggctgttt
      241 cgccgctgtc gaacagcagg gctccgatca ggttcttctt gatgctgtgc cggtcggtgt
      301 tgcccagcac cttgaatttc ttgctgggca ccttgtactc gtcggtgatc acggcccagc
      361 ccacagagtt ggtgccgatg tccaggccga tgctgtactt cttgtcgact ttccgcttct
      421 tctttggtga ctcgaactcg cttccgtcgg ctgtccgttt catggtggct ctagaaccgg
      481 tcctgtgttc tggcggcaaa cccgttgcga aaaagaacgt tcacggcgac tactgcactt
      541 atatacggtt ctcccccacc ctcgggaaaa aggcggagcc agtacacgac atcactttcc
      601 cagtttaccc cgcgccacct tctctaggca ccggatcaat tgccgacccc tccccccaac
      661 ttctcgggga ctgtgggcga tgtgcgctct gcccactgac gggcaccgga gccaattccc
      721 actcctttca agacctagct agctaattaa ccaaactgga tctctgctgt ccctgtaata
      781 aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt
      841 tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt
      901 aaaattgtgg atgaatactg ccatttgtct gcagaattgg cgcacgcagt gccgatccgt
      961 tcactaatcg aatggatctg tctctgtctc tctctccacc ttcttcttct attccttcgg
     1021 gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaatatccct
     1081 gcctaactct attcactata gaaagtacag caaaaactat tcttaaacct accaagcctc
     1141 ctactatcat tatgaataat tttatatacc acagccaatt tgttatgtta aaccaattcc
     1201 acaaacttgc ccatttatct aattccaata attcttgttc attcttttct tgctggtttt
     1261 gcgattcttc aattaaggag tgtattaagc ttgtgtaatt gttaatttct ctgtcccact
     1321 ccatccaggt cgtgtgattc caaatctgtt ccagagattt attactccaa ctagcattcc
     1381 aaggcacagc agtggtgcaa atgagttttc cagagcaacc ccaaatcccc aggagctgtt
     1441 gatcctttag gtatctttcc acagccagga ttcttgcctg gagctgcttg atgccccaga
     1501 ctgtgagttg caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgct
     1561 gcactatacc agacaataat tgtctggcct gtaccgtcag cgtcattgac gctgcgccca
     1621 tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc tcctattccc actgctcttt
     1681 tttctctctg caccactctt ctctttgcct tggtgggtgc tactcctaat ggttcaattt
     1741 ttactacttt atatttatat aattcacttc tccaattgtc cctcatatct cctcctccag
     1801 gtctgaagat cagcggccgc ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct
     1861 atcttgtcta aagcttcctt ggtgtctttt atctctatcc tttgatgcac acaatagagg
     1921 gttgctactg tattatataa tgatctaagt tcttctgatc ctgtctgaag ggatggttgt
     1981 agctgtccca gtatttgtct acagccttct gatgtttcta acaggccagg attaactgcg
     2041 aatcgttcta gctccctgct tgcccatact atatgtttta atttatattt tttctttccc
     2101 cctggcctta accgaatttt ttcccatcgc gatctaattc tcccccgctt aatactgacg
     2161 ctctcgcacc catctctctc cttctagcct ccgctagtca aaatttttgg cgtactcacc
     2221 agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag
     2281 agctcctctg gtttcccttt cgctttcaag tccctgttcg ggcgccactg ctagagattt
     2341 tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg
     2401 cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta
     2461 gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca gtacaggcaa
     2521 aacgcgctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg
     2581 gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca
     2641 ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca
     2701 ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac
     2761 tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta
     2821 ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag
     2881 tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt
     2941 actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca
     3001 ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat
     3061 gaccccgtaa ttgattacta ttaataacta gtcaataatc aatgtcaacg cgtatatctg
     3121 gcccgtacat cgcgaagcag cgcaaaacgc ctaaccctaa gcagattctt catgcaattg
     3181 tcggtcaagc cttgccttgt tgtagcttaa attttgctcg cgcactactc agcgacctcc
     3241 aacacacaag cagggagcag atactggctt aactatgcgg catcagagca gattgtactg
     3301 agagtgcacc ataggggatc gggagatctc ccgatccgtc gacgtcaggt ggcacttttc
     3361 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     3421 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     3481 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     3541 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     3601 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     3661 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     3721 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     3781 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     3841 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     3901 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     3961 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     4021 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     4081 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     4141 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     4201 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     4261 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     4321 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     4381 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     4441 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4501 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4561 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4621 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     4681 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4741 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4801 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4861 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4921 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4981 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     5041 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     5101 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     5161 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     5221 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     5281 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     5341 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     5401 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     5461 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagctctagc
     5521 tagaggtcga cggtatacag acatgataag atacattgat gagtttggac aaaccacaac
     5581 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     5641 aaccattata agctgcaata aacaagttgg ggtgggcgaa gaactccagc atgagatccc
     5701 cgcgctggag gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat
     5761 agaaggcggc ggtggaatcg aaatctcgta gcacgtgtca gtcctgctcc tcggccacga
     5821 agtgcacgca gttgccggcc gggtcgcgca gggcgaactc ccgcccccac ggctgctcgc
     5881 cgatctcggt catggccggc ccggaggcgt cccggaagtt cgtggacacg acctccgacc
     5941 actcggcgta cagctcgtcc aggccgcgca cccacaccca ggccagggtg ttgtccggca
     6001 ccacctggtc ctggaccgcg ctgatgaaca gggtcacgtc gtcccggacc acaccggcga
     6061 agtcgtcctc cacgaagtcc cgggagaacc cgagccggtc ggtccagaac tcgaccgctc
     6121 cggcgacgtc gcgcgcggtg agcaccggaa cggcactggt caacttggcc atggtttagt
     6181 tcctcacctt gtcgtattat actatgccga tatactatgc cgatgattaa ttgtcaacac
     6241 gtgctgatca gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag
     6301 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc
     6361 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
     6421 agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc
     6481 tgctggggag cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg
     6541 catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca
     6601 cagaattaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     6661 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     6721 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     6781 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     6841 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     6901 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     6961 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     7021 ccgctacagg gcgcgtgggg atacccccta gagccccagc tggttctttc cgcctcagaa
     7081 gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc ctcccccttg
     7141 ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca
     7201 atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc
     7261 acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga ggctgatcag
     7321 cgggtttaaa cgggccctgc tagagatttt ccacactgac taaaagggtc tgagggatct
     7381 ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct
     7441 ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg
     7501 gtctaaccag agagacccag tacagtccgg atgcagctct cgggccatgt gatgaaatgc
     7561 taggcggctg tcaaacctcc actctaatac ttctctctcc gggtcatcca tcccatgcag
     7621 gctcacaggg tgtaacaagc gggtgttctc tccttcattg gcttcttcta ccttctcttg
     7681 ctcaactggt actagcttgt agcaccatcc aaaggtcagt ggatatctga tccctggccc
     7741 tggtgtgtag ttctgccaat cagggaagta gccttgtgtg tggtagatcc acagatcaag
     7801 gatatcttgt cttcgttggg agtgaattag cccttccagt cccccctttt cttttaaaaa
     7861 gtggctaaga tctacagctg ccttgtaagt cattggtctt aaaggtacct gaggtgtgac
     7921 tggaaaaccc acctcctcct cctcttgtgc ttctagccag gcacaatcag cattggtagc
     7981 tgctgtattg ctacttgtga ttgctccatg tttttctagg tctcgaggtc gacggtatcg
     8041 atgcggggag gcggcccaaa gggagatccg actcgtctga gggcgaaggc gaagacgcgg
     8101 aagaggccgc agagccggca gcaggccgcg ggaaggaagg tccgctggat tgagggccga
     8161 agggacgtag cagaaggacg tcccgcgcag aatccaggtg gcaacacagg cgagcagcca
     8221 aggaaaggac gatgatttcc ccgacaacac cacggaattg tcagtgccca acagccgagc
     8281 ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa tagggagggg
     8341 gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca atgccccaac cagtgggggt
     8401 tgcgtcagca aacacagtgc acaccacgcc acgttgcctg acaacgggcc acaactcctc
     8461 ataaagagac agcaaccagg atttatacaa ggaggagaaa atgaaagcca tacgggaagc
     8521 aatagcatga tacaaaggca ttaaagcagc gtatccacat agcgtaaaag gagcaacata
     8581 gttaagaata ccagtcaatc tttcacaaat tttgtaatcc agaggttgat taccgataag
     8641 cttgatatcg accggttagc cctcccacac ataaccagag ggcagcaatt cacgaatccc
     8701 aactgccgtc ggctgtccat cactgtcctt cactatggct ttgatcccag gatgcagatc
     8761 gagaagcacc tgtcggcacc gtccgcaggg gctcaagatg cccctgttct catttccgat
     8821 cgcgacgata caagtcaggt tgccagctgc cgcagcagca gcagtgccca gcaccacgag
     8881 ttctgcacaa ggtcccccag taaaatgata tacattgaca ccagtgaaga tgcggccgtc
     8941 gctagagaga gctgcgctgg cgacgctgta gtcttcagag atggggatgc tgttgattgt
     9001 agccgttgct ctttcaatga gggtggattc ttcttgagac aaaggcttgg ccatcggtcc
     9061 aggattctct tcgacatctc cggcttgttt cagcagagag aagtttgttg cgccggatcc
     9121 gtccagcttc actctcttag cggcagggcc agagccgact ttcctcttct tcttgggaga
     9181 ctcgaattcg ctgccgtcgg cggttctttt tgagccgcca gaaggggagg agttttcaat
     9241 cagcagagtg gaagtgtcgg gagtttcagt aatagcagcc tttctggcgg cctggtcggc
     9301 catccgattg ccccttgcct cggcggagtg tcccttctgg tgtccaggac agtggatgat
     9361 gctcagccgc ttgggcagaa acagggcctt cagcagggcc aggatctcat ccttgttctt
     9421 gatctccttg ccctcagagg tcagccagcc ccggcgcctg tagatctctc cgtggatgtg
     9481 tgcggtggcg aaggcatatc tggagtctgt gtacacgttc agcttcttgc cctcggccat
     9541 cttcagggcc tgggtcaggg cgatcagctc ggcccgctgg gcggatgtgc ctgctggcag
     9601 ggccttggcc cagatcactt ctgtctctgt ggtcactgct gctcctgcct tcctctggcc
     9661 ctcctgcagc agggagcttc catcggtata ccatgtgtga tcggcgtcag gcaggggctg
     9721 atcggtcagg tctggccttg ttccgtgtgc ctctgccagg atgtccagac agttgtgctg
     9781 caggccctcc tctggcagag gcagcagggt ggctggattc agggccacca cagggccaaa
     9841 ctgcacgcga tcggtgtcca gcagcagggc ctggtagtgt gtcatccggg cgttagacag
     9901 ccagcgatct ggaggctgct tcaccagggc ctccactgcg tgtggtgcca ggatgaccag
     9961 tggctgtccc atggtcagct tgccggcatc ctttgtcagc acggcgattg ctgccaccat
    10021 cctcaggcat ggtggccatc ctgctgccac tgggtccagc ttcttggaca gataggccac
    10081 gggccgtctc catggtccca gcttctgggt cagcacgccc ttggcgtagc cctgcttctc
    10141 atccacaaac agctcgaaag gcttggtcag gtctggcagg cccagggctg gtgctgtcag
    10201 cagggcctgc ttgatctcct gataggcctt ctgctggtca gggccccaat taaacagggt
    10261 gcccggcttg gtcagtgggt acagaggtgc tgccatctct gcgaatcctg ggatgaacag
    10321 cctgcaaaat cctgctttgc ccaggaactc cctcagctgt cttggggtct tgggtgttgg
    10381 ctggcccatc actgtctcct tcctggcctc tgtcagccat ctctggccct ccttcagcag
    10441 atagcccagg tacttcacct gcttctgaca gatctgggcc ttcttggcgg atgccctata
    10501 tcccaggttt cccagggtct gcagcagggc gcgtgttccc tgctggcaat ccagctcgct
    10561 ggtggcggcc agcagcagat cgtccacgta ctgcagcagg atcagatctg ggtgctggat
    10621 ccggaaatct gccaggtcgc ggtgcagggc ctcgttaaac agtgttgggc tattcttgaa
    10681 gccctgaggc aggcgtgtcc aggtcagctg gccagagatg cccatctcag ggtccctcca
    10741 ctcaaaggcg aacagtggct ggctggtagg gtgcagtctc agacaaaaga aggcatcctt
    10801 caggtccagc actgtatacc actggtgaga agggggcagg ccggacagca gattgtaagg
    10861 gttgggcacg gtggggtgga tatcctccac cctcttgttc acctctctca gatcctgcac
    10921 gggtctatag tcattggtgc ctggcttctt cacgggcagc agtggtgtgt tccaggggct
    10981 ctggcatggc accaggatgc cctggtccag cagcctctgg atgtgaggct tgatgcccag
    11041 tctggcctcc tgagacatag ggtactgctt gatgctcacg ggtgtagagg tggccttcag
    11101 agggatgatc aggggtgcct gccgcacggc caggcccatt cctcctgtct cggcccaggc
    11161 ctggggaaag tcggacagcc aggtggagcc cagggacacg tcgggttcct tgcttgtttc
    11221 atgcagtcta tactcgtctt caatgttcag ggtgcttccg ccgctagagc ctccgctcac
    11281 ctttcttttc ttcttagggc tctcgaattc agagccgtcg gcggtccgct tgctgccacc
    11341 agagcttccg ccggagtcac ctcccagctg agacaggtcg atccgtgtct cgtacaggcc
    11401 ggtgatgctc tggtggatca gggtggcgtc cagcacctct ttggtgctgg tgtacctctt
    11461 ccggtcgatg gtggtgtcaa agtacttgaa ggcggcaggg gctcccagat tggtcagggt
    11521 aaacaggtgg atgatattct cggcctgctc tctgatgggc ttatcccggt gcttgttgta
    11581 ggcggacagc actttgtcca gattagcgtc ggccaggatc actctcttgg agaactcgct
    11641 gatctgctcg atgatctcgt ccaggtagtg cttgtgctgt tccacaaaca gctgtttctg
    11701 ctcattatcc tcgggggagc ccttcagctt ctcatagtgg ctggccaggt acaggaagtt
    11761 cacatatttg gagggcaggg ccagttcgtt tcccttctgc agttcgccgg cagaggccag
    11821 cattctcttc cggccgtttt ccagctcgaa cagggagtac ttaggcagct tgatgatcag
    11881 gtcctttttc acttctttgt agcccttggc ttccagaaag tcgatgggat tcttctcgaa
    11941 gctgcttctt tccatgatgg tgatccccag cagctctttc acactcttca gtttcttgga
    12001 cttgcccttt tccactttgg ccaccaccag cacagaatag gccacggtgg ggctgtcgaa
    12061 gccgccgtac ttcttagggt cccagtcctt ctttctggcg atcagcttat cgctgttcct
    12121 cttgggcagg atagactctt tgctgaagcc gcctgtctgc acctcggtct ttttcacgat
    12181 attcacttgg ggcatgctca gcactttccg cacggtggca aaatcccggc ccttatccca
    12241 cacgatctcc ccggtttcgc cgtttgtctc gatcagaggc cgcttccgga tctcgccgtt
    12301 ggccagggta atctcggtct tgaaaaagtt catgatgttg ctgtagaaga agtacttggc
    12361 ggtagccttg ccgatttcct gctcgctctt ggcgatcatc ttccgcacgt cgtacacctt
    12421 gtagtcgccg tacacgaact cgctttccag cttagggtac tttttgatca gggcggttcc
    12481 cacgacggcg ttcaggtagg cgtcgtgggc gtggtggtag ttgttgatct cgcgcacttt
    12541 gtaaaactgg aaatccttcc ggaaatcgga caccagcttg gacttcaggg tgatcacttt
    12601 cacttcccgg atcagcttgt cattctcgtc gtacttagtg ttcatccggg agtccaggat
    12661 ctgtgccacg tgctttgtga tctgccgggt ttccaccagc tgtctcttga tgaagccggc
    12721 cttatccagt tcgctcaggc cgcctctctc ggccttggtc agattgtcga actttctctg
    12781 ggtaatcagc ttggcgttca gcagctgccg ccagtagttc ttcatcttct tcacgacctc
    12841 ttcggagggc acgttgtcgc tcttgccccg gttcttgtcg cttctggtca gcaccttgtt
    12901 gtcgatggag tcgtccttca gaaagctctg aggcacgata gcgtccacat cgtagtcgga
    12961 cagccggttg atgtccagtt cctggtccac gtacatatcc cgcccattct gcaggtagta
    13021 caggtacagc ttctcgttct gcagctgggt gttttccacg gggtgttctt tcaggatctg
    13081 gctgcccagc tctttgatgc cctcttcgat ccgcttcatt ctctcgcggc tgttcttctg
    13141 tcccttctgg gtggtctggt tctctctggc catttcgatc acgatgttct cgggcttgtg
    13201 ccggcccatc actttcacga gctcgtccac caccttcact gtctgcagga tgcccttctt
    13261 aatggcgggg ctgccggcca gattggcaat gtgctcgtgc aggctatcgc cctggccgga
    13321 cacctgggct ttctggatgt cctctttaaa ggtcaggctg tcgtcgtgga tcagctgcat
    13381 gaagtttctg ttggcgaagc cgtcggactt caggaaatcc aggattgtct tgccggactg
    13441 cttgtcccgg atgccgttga tcagcttccg gctcagcctg ccccagccgg tgtatctccg
    13501 ccgcttcagc tgcttcatca ctttgtcgtc gaacaggtgg gcataggttt tcagccgttc
    13561 ctcgatcatc tctctgtcct caaacagtgt cagggtcagc acgatatctt ccagaatgtc
    13621 ctcgttttcc tcattgtcca ggaagtcctt gtccttgata attttcagca gatcgtggta
    13681 tgtgcccagg gaggcgttga accgatcttc cacgccggag atttccacgg agtcgaagca
    13741 ctcgattttc ttgaagtagt cctctttcag ctgcttcacg gtcactttcc ggttggtctt
    13801 gaacagcagg tccacgatgg cctttttctg ctcgccgctc aggaaggcgg gctttctcat
    13861 tccctcggtc acgtatttca ctttggtcag ctcgttatac acggtgaagt actcgtacag
    13921 caggctgtgc ttgggcagca ccttctcgtt gggcaggttc ttatcgaagt tggtcatccg
    13981 ctcgatgaag ctctgggcgg aagcgccctt gtccaccact tcctcgaagt tccagggggt
    14041 gatggtttcc tcgctctttc tggtcatcca ggcgaatctg ctgtttcccc tggccagagg
    14101 gcccacgtag taggggatgc ggaaggtcag gatcttctcg atcttttccc ggttgtcctt
    14161 caggaatggg taaaaatctt cctgccgccg cagaatggcg tgcagctctc ccaggtggat
    14221 ctggtggggg atgctgccgt tgtcgaaggt ccgctgcttc cgcagcaggt cctctctctt
    14281 cagcttcacg agcagttcct cggtgccgtc catcttttcc aggatgggct tgatgaactt
    14341 gtagaactct tcctggctgg ctccgccgtc aatgtagccg gcgtagccgt tcttgctctg
    14401 gtcgaagaaa atctctttgt acttctcagg cagctgctgc cgcacgagag ctttcagcag
    14461 ggtcaggtcc tggtggtgct cgtcgtatct cttgatcata gaggcgctca ggggggcctt
    14521 ggtgatctcg gtgttcactc tcaggatgtc gctcagcagg atggcgtcgg acaggttctt
    14581 ggcggccaga aacaggtcgg cgtactggtc gccgatctgg gccagcaggt tgtccaggtc
    14641 gtcgtcgtag gtgtccttgc tcagctgcag tttggcatcc tcggccaggt cgaagttgct
    14701 cttgaagttg ggggtcaggc ccaggctcag ggcaatcagg tttccgaaca ggccattctt
    14761 cttctcgccg ggcagctggg cgatcagatt ttccagcttt ctgctcttgc tcagtctggc
    14821 agacaggatg gccttggcgt ccacgccgct ggcgttgatg gggttttcct cgaacagctg
    14881 gttgtaggtc tgcaccagct ggatgaacag cttgtccacg tcgctgttgt cggggttcag
    14941 gtcgccctcg atcaggaagt ggccccggaa cttgatcatg tgggccaggg ccagatagat
    15001 cagccgcagg tcggccttgt cggtgctgtc caccagtttc tttctcaggt
//
