LOCUS       sequence-47353-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Shibue T, Weinberg RA
  TITLE     Integrin beta1-focal adhesion kinase signaling directs the 
            proliferation of metastatic cancer cells disseminated in the lungs.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Jun 23;106(25):10290-5. Epub 2009 Jun
            5.
  PUBMED    19502425
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2009-06-23"; volume: "106(25)"; pages: 
            "10290-5. Epub 2009 Jun 5"
COMMENT     Sequence Label: pLKO-puro-sh-mFAK B
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    125..242
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        291..801
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     720..738
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(824..843)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 atatcgttgt ggaaaggacg aaacaccggc ctggcatctt tgatattata ctcgagttat
       61 aatatcaaag atgccaggtt tttgaattct cgacctcgag acaaatggca gtattcatcc
      121 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
      181 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
      241 ttcgggttta ttacagggac agcagagatc cactttggcc gcggctcgag ggggttgggg
      301 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
      361 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
      421 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
      481 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
      541 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
      601 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
      661 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
      721 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
      781 atcaccgacc tctctcccca gggggatcca ccggagcttt accatgaccg agtacaagcc
      841 cacggtgcgc ctcgccaccc gcgacgacgt ccccaggggc cgtacgcacc ctcgccgccg
      901 cgttcgcc
//
