LOCUS       sequence-473532-        8217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ABE9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   Chen L, Zhang S, Xue N, Hong M, Zhang X, Zhang D, Yang J, Bai S, 
            Huang Y, Meng H, Wu H, Luan C, Zhu B, Ru G, Gao H, Zhong L, Liu M, 
            Liu M, Cheng Y, Yi C, Wang L, Zhao Y, Song G, Li D
  TITLE     Engineering a precise adenine base editor with minimal bystander 
            editing.
  JOURNAL   Nat Chem Biol. 2022 Oct 13. pii: 10.1038/s41589-022-01163-8. doi: 
            10.1038/s41589-022-01163-8.
  PUBMED    36229683
REFERENCE   2  (bases 1 to 8217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2022 Oct 13. pii: 10.1038/s41589-022-01163-8. doi: 
            10.1038/s41589-022-01163-8."
COMMENT     Sequence Label: ABE9
FEATURES             Location/Qualifiers
     source          1..8217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3533..3553
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3562..3579
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3602..3619)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3608..3832
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3903..3919)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3903..3919)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3916..3938)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3927..3943
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3951..3957,3958..3975,3976..3981))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3996..4017
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4134..4151)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4305..4893)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4385..4404)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5064..5855,5856..5924))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5687..5706
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5925..6029)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6108..6127)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6299..6678
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6679..6882
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6833..6853
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6924..6943
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6924..6942
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6992..7012
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7607..7630,7631..7633,7634..8217,1..3490)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(7607..8217,1..3490)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct
       61 gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggaaacctga ttgccctgag
      121 cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca
      181 gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca
      241 gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc tgagcgacat
      301 cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga tcaagagata
      361 cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc agctgcctga
      421 gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggct acattgacgg
      481 cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa agatggacgg
      541 caccgaggaa ctgctcgtga agctgaacag agaggacctg ctgcggaagc agcggacctt
      601 cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca ttctgcggcg
      661 gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga agatcctgac
      721 cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat tcgcctggat
      781 gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg tggacaaggg
      841 cgcttccgcc cagagcttca tcgagcggat gaccaacttc gataagaacc tgcccaacga
      901 gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtata acgagctgac
      961 caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa
     1021 aaaggccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa
     1081 agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga
     1141 tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga
     1201 cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact
     1261 gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga
     1321 caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa
     1381 gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc
     1441 cgacggcttc gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa
     1501 agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc
     1561 caatctggcc ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga
     1621 cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag
     1681 agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga
     1741 agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca
     1801 gctgcagaac gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga
     1861 ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gaccatatcg tgcctcagag
     1921 ctttctgaag gacgactcca tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg
     1981 caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcggca
     2041 gctgctgaac gccaagctga ttacccagag aaagttcgac aatctgacca aggccgagag
     2101 aggcggcctg agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg
     2161 gcagatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga
     2221 gaatgacaag ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga
     2281 tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca
     2341 cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga
     2401 aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag
     2461 cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt
     2521 tttcaagacc gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac
     2581 aaacggcgaa accggggaga tcgtgtggga taagggccgg gattttgcca ccgtgcggaa
     2641 agtgctgagc atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt
     2701 cagcaaagag tctatcctgc ccaagaggaa cagcgataag ctgatcgcca gaaagaagga
     2761 ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt ctgtgctggt
     2821 ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctgctggg
     2881 gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa
     2941 gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga
     3001 gctggaaaac ggccggaaga gaatgctggc ctctgccggc gaactgcaga agggaaacga
     3061 actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct
     3121 gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc acaagcacta
     3181 cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc
     3241 taatctggac aaagtgctgt ccgcctacaa caagcaccgg gataagccca tcagagagca
     3301 ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg ccgccttcaa
     3361 gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg tgctggacgc
     3421 caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct
     3481 gggaggtgac tctggcggct caaaaagaac cgccgacggc agcgaattcg agcccaagaa
     3541 gaagaggaaa gtctaaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca
     3601 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     3661 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     3721 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     3781 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     3841 gcggaaagaa ccagctgggg ctcgataccg tcgacctcta gctagagctt ggcgtaatca
     3901 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     3961 gccggaagca taaagtgtaa agcctagggt gcctaatgag tgagctaact cacattaatt
     4021 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     4081 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     4141 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     4201 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     4261 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     4321 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     4381 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     4441 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     4501 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4561 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4621 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     4681 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     4741 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     4801 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     4861 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4921 tctgacactc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     4981 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     5041 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     5101 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     5161 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     5221 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     5281 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     5341 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     5401 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     5461 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     5521 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     5581 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     5641 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     5701 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     5761 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     5821 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     5881 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     5941 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     6001 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     6061 gacggatcgg gagatcgatc tcccgatccc ctagggtcga ctctcagtac aatctgctct
     6121 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     6181 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     6241 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
     6301 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     6361 tatattgagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     6421 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     6481 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     6541 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     6601 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     6661 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     6721 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     6781 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     6841 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag
     6901 atccgctaga gatccgcggc cgctaatacg actcactata gggagagccg ccaccatgaa
     6961 acggacagcc gacggaagcg agttcgagtc accaaagaag aagcggaaag tctctgaggt
     7021 ggagttttcc cacgagtact ggatgagaca tgccctgacc ctggccaaga gggcacggga
     7081 tgagagggag gtgcctgtgg gagccgtgct ggtgctgaac aatagagtga tcggcgaggg
     7141 ctggaacaga gccatcggcc tgcacgaccc aacagcccat gccgaaatta tggccctgag
     7201 acagggcggc ctggtcatgc agaactacag actgattgac gccaccctgt acgtgacatt
     7261 cgagccttgc gtgatgtgcg ccggcgccat gatccactct aggatcggcc gcgtggtgtt
     7321 tggcgtgagg cagtcaaaaa gaggcgccgc aggctccctg atgaacgtgc tgaactaccc
     7381 cggcatgaat caccgcgtcg aaattaccga gggaatcctg gcagatgaat gtgccgccct
     7441 gacctgcgat ttctatcgga tgcctagaca ggtgttcaat gctcagaaga aggcccagag
     7501 ctccatcaac tccggaggat ctagcggagg ctcctctggc tctgagacac ctggcacaag
     7561 cgagagcgca acacctgaaa gcagcggggg cagcagcggg gggtcagaca agaagtacag
     7621 catcggcctg gccatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa
     7681 ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa
     7741 cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag
     7801 aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt
     7861 cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag agtccttcct
     7921 ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt
     7981 ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac
     8041 cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg
     8101 ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat
     8161 ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcgg
//
