LOCUS       sequence-473540-       10325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to express GFP-3xNLS fused to 3xHA-TurboID (N-terminal) in T. 
            cruzi. Tetracycline regulated. Used as proximity labelling nuclear 
            spatial control..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10325)
  TITLE     Serra Lab Plasmids
REFERENCE   2  (bases 1 to 10325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTcTurboID-GW_GFP-NLS
FEATURES             Location/Qualifiers
     source          1..10325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2866..3891)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(2866..3891)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(4363..4381)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(4414..4461)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      complement(4546..4593)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        4621..4639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4646..4664
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             4923..4949
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4953..4979
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4983..5009
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5016..5972
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    6018..6042
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6075..6788
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFNDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(6105..6133)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6728..6748
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             6831..6851
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6855..6875
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6879..6899
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(6917..6941)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      7473..7520
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      7605..7652
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(7699..7715)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7699..7715)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7712..7734)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7723..7739
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7747..7753,7754..7771,7772..7777))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7792..7813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7930..7947)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8101..8689)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8181..8200)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8860..9651,9652..9720))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9721..9825)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9893..9911
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9949..9971)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10071..10090
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10284..10306
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10298..10315
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10299..10315
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcatttgtgt ctagtacatc atggagcggt attctctccg gtggcccagg tggcgtcgga
       61 aggaattggt gtccctcagg atgtttggtt tttcgttttt ttttttgcgg tcttttgttt
      121 cgcatcgccg tatcgtgtgc ggcgcgacct tctttttggc tcattggtca gtgaggttta
      181 taataaagtg gatcacttgg gtttttggcc ctttgcggcg cacccgcgga atgggggagg
      241 gattttcata atgtagtgta cattgtgtgt gggtcgcaaa atgtcatgcc gtgtgcaatg
      301 tttatttgcg tgctgtgcgt gtccttttgt gattcccacc acttgtgtgt attttacctt
      361 atcgtctctc cctgccgttg gctgtgcgtg aattgtgctt cgcattcgtg ggaaatcaaa
      421 aggggaatca acgtgttctt ttttttcaga aggggagtca aagcgccgtg tggatgccaa
      481 gtctgtgcaa aagttaacta ttattactac tgctgctgct gctgccactc ctacatatgc
      541 gcacaagtac actatatata tgcgaataca cctgtgtgta ttatgctatg tgtgtgtctg
      601 tgtgggtgtg taggtgatgg ggtagtggtt gtattatagt atagtatata tagtagtata
      661 cggcaaaagg caaagacaca agcacggcga gtacgacgaa caaacccatt tcacacatga
      721 gtgtgcgcca cacacgcgac tgctcctttt ttttctgcct tgccaccacc ccatatgtat
      781 attatatata tatatatata agggtgtgtg tgtggtgaga gaaaatagga aatgattgcg
      841 tgtgtgtcgc cgccacgaac ctgtcagatg ctttcttacg gtattttgtt gcatgcaccc
      901 gcgtacacac aatatacaca cacaaaaaaa acaccgatat gatggcgagt gtgcgattct
      961 ccttttattg tctttttcct ccttttctgt gctctctgct gtgtgccaat aaaacacact
     1021 cctcctatgt ttggtgtagt gtgtgttatt tattgtccgc gcaaaaaaaa aagcgagtag
     1081 agaaacgggg gaaaaagtag taaataaatg gaggactgct tcgcagctgc cctgccgcac
     1141 tgtttttgct gctgggttcg caacgcgatg ctgttgagag tgaaatcaca tcacaaaaag
     1201 cacccctcag actggtgtga agtgtgaagt atgtgtacaa atgcgtgtgt gtgtgtatac
     1261 acattgacga atagcatacc agggaaaaaa gggaggacac tctctttcgt gcgtgctgaa
     1321 ctctgtgtgt gtgtgctgcc gttttgtttg cagcttgaaa tgcttggttg aatagaaatc
     1381 ggtacttgat tgttgccgca tttcgaggaa tttcatccaa aaaaaaaaag aggtcaacaa
     1441 caaccaactg acaaactttg tgttggacct gtgtatgtgg tggtgtggtg ggggaagaga
     1501 aacacacaca cagacaaaca caaacaccat gtcattttgg catgcgtgtg cgtgcgtgcg
     1561 tgcgttgaat atcctccaca cacaaatata tatatataat aataccacaa caacatttca
     1621 aaggatcatc aatgtgtgtg agacgtgtgc gtttgtagcg tgtgtgtata tgtgttttgt
     1681 gtgttgtgtg gtgtaatgtg tgtgtgtgtc gttcgtgcat atacacctta tacacacaca
     1741 catataatac atacataaat acacacacac ggcgaacgcc gcggaccaac aacttgagat
     1801 ctggttgatt ctgccagtag tcatatgctt gtttcaagga cttagccatg cacgcctcag
     1861 actagtagct cggtgcaccc tgggatattt gcgcacccac ctttcccgtc gcaatcggcg
     1921 ccgtccgaac gcggaaatat tccgagaata atacggctgg gctgcgccgc gcgttccccg
     1981 cgtgaaacaa ttattgtgcg atgtgcagca tcctattgtg gtgctttacc gcacaccagt
     2041 ttccttgcgg ggcttatttg gtgtgtcgga tttttaattt tttgatgatt cggaggaatt
     2101 ttttacaatt tttgggtacc cctaaaaaac tttttggagt ttccggggtt cgatccaact
     2161 tgtgcttttt taaccgtcca tttgtgtgag ctccaccgcg gtggcggccg atcccccggg
     2221 ctgcaggaat tcgatccaca cggctagcat actctagcgg catatacaca ggtgggctat
     2281 aatgcccagt attgttcgaa aaagtccaaa gaacaacaac actacgacgc ataatgcaca
     2341 aacactaaac tacaccttaa acaccatcag tgaagaaaaa gaaaaaaaaa gaaaaacagc
     2401 ttaaatcctt aaaaaaaaaa aatcacacta aattaaaaca gtcagttata aacaaaaaga
     2461 aactgaacaa ccaaaatctt ccaataacgc aaagttgtaa aaaattacca caagctgcat
     2521 catacaccca atcaaatcat ctacattttc cttgcaatac cccaaatcct cgtacattga
     2581 ttaaacatta tttcactcat ccttccgcat cattaaattg ctgctttttg tttcccaaag
     2641 atgaccataa atataatttc aaaaaaaaaa aagaaaaaaa agttatatat ttttttgttg
     2701 ttgtccttaa agggaaaagt aaaattgttt tgctcttgac actgtgtcac ttggatcgtg
     2761 gcagcaccgg taacgatcat cacgggacac gcatcgtgta ttataagatc cctattcgca
     2821 aaagaaagac ctgawstcrw cgmyasrycy msrwcskcrt ctactctatt cctttgccct
     2881 cggacgagtg ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt
     2941 ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg
     3001 gacgattgcg tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa
     3061 gctctgatag agttggtcaa gaccaatgcg gagcatatac gcccggagcc gcggcgatcc
     3121 tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca
     3181 cggcctccag aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct
     3241 ccagtcaatg accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc
     3301 cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag
     3361 agcctgcgcg acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg
     3421 gggatcagca atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg
     3481 tccgaatggg ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatggc
     3541 ctccgcgacc ggctgcagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac
     3601 accctgtgca cggcgggaga tgcaataggt caggctctcg ctaaattccc caatgtcaag
     3661 cacttccgga atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta
     3721 gaaaccatcg gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct
     3781 gaaagcacga gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgtcgaactt
     3841 ttcgatcaga aacttctcga cagacgtcgc ggtgagttca ggctttttca tatctcattg
     3901 ccccccggga tcagatctgc agggcttccc aaccttacca gagggcgccc cagctggcaa
     3961 gctagttcta gaattctgtt caatgtaatt gttgttggta gatggctttc aaagtaaaaa
     4021 tatcactttc tgcattcgtg caggaaacgt gggaaaagag agacaagtgg aatgaagggg
     4081 aggttgacaa ttgtgggttt tgtgtttctt ttcttgcatt catttacggc cgctgtaatt
     4141 attattatta ttattattat ttgtagagga cagacataaa ataaatttga ggtattttcc
     4201 gcattttgtt tttgtttgtt tgtttgcttc tgaatgcaat gagaatttgt ttgtgctctt
     4261 gtctaataag actgttgtgt ctgttttttt gagtctcctg aagaaactgg caactgttga
     4321 acttcgctca cttcctgaat gcggatacga tagatgaaat gccctatagt gagtcgtatt
     4381 acaaggtacc ccggatatag ttcctccttt cagcaaaaaa cccctcaaga cccgtttaga
     4441 ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact cagcttcctt
     4501 tcgggctttg ttagcagccc tatccggata tagttctcct ttcagcaaaa aacccctcaa
     4561 gacccgttta gaggccccaa ggggttatgc tagttattgc tcagcgggct agcacctgat
     4621 taatacgact cactataggg agatctccct atcagtgata gagatctagc ccctcgagat
     4681 cccccaacga gtttcttcaa aatatgcagc ggattcacta agaaacattt tcacgcacga
     4741 aagcgaaatt attatgattg ttattataat actttttctt tgttgtttta tccacttatt
     4801 attgttgtgt taaattgttt ttaccttttt tcttttccaa cttcttttat gatgtctttt
     4861 cttttttttt ttggctctat aagttgtctt gcggccgccg gcatcgattg gccgccacca
     4921 tgtacccgta tgatgttccg gattacgctg gctatcccta cgacgtgccc gactatgccg
     4981 ggtaccccta tgacgtccca gactacgcag ctagcaaaga caatactgtg cctctgaagc
     5041 tgatcgctct cctggctaat ggcgagttcc atagtggcga acagctggga gaaaccctgg
     5101 gcatgtccag ggccgctatc aacaagcaca ttcagactct gcgcgactgg ggcgtggacg
     5161 tgttcaccgt gcccggaaag ggctactctc tgcccgagcc tatcccgctg ctgaacgcta
     5221 aacagattct gggacagctg gacggcggga gcgtggcagt cctgcctgtg gtcgactcca
     5281 ccaatcagta cctgctggat cgaatcggcg agctgaagag tggggatgct tgcattgcag
     5341 aatatcagca ggcagggaga ggaagcagag ggaggaaatg gttctctcct tttggagcta
     5401 acctgtacct gagtatgttt tggcgcctga agcggggacc agcagcaatc ggcctgggcc
     5461 cggtcatcgg aattgtcatg gcagaagcgc tgcgaaagct gggagcagac aaggtgcgag
     5521 tcaaatggcc caatgacctg tatctgcagg atagaaagct ggcaggcatc ctggtggagc
     5581 tggccggaat aacaggcgat gctgcacaga tcgtcattgg cgccgggatt aacgtggcta
     5641 tgaggcgcgt ggaggaaagc gtggtcaatc agggctggat cacactgcag gaagcaggga
     5701 ttaacctgga caggaatact ctggccgcta cgctgatccg agagctgcgg gcagccctgg
     5761 aactgttcga gcaggaaggc ctggctccat atctgccacg gtgggagaag ctggataact
     5821 tcatcaatag acccgtgaag ctgatcattg gggacaaaga gattttcggg attagccggg
     5881 ggattgataa acagggagcc ctgctgctgg aacaggacgg agttatcaaa ccctggatgg
     5941 gcggagaaat cagtctgcgg tctgccgaaa aggaattcag cagggccgac gaatcgatac
     6001 gcgtggatcc gtcgatcaca agtttgtaca aaaaagcagg ctctttaaag gaaccaattc
     6061 agtcgactgg atccatgagt aaaggagaag aacttttcac tggagttgtc ccaattcttg
     6121 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
     6181 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
     6241 catggccaac acttgtcact actttcactt atggtgttca atgcttttca agatacccag
     6301 atcatatgaa gcggcacgac ttcttcaaga gcgccatgcc tgagggatac gtgcaggaga
     6361 ggaccatctt cttcaatgac gacgggaact acaagacacg tgctgaagtc aagtttgagg
     6421 gagacaccct cgtcaacagg atcgagctta agggaatcga tttcaaggag gacggaaaca
     6481 tcctcggcca caagttggaa tacaactaca actcccacaa cgtatacatc atggccgaca
     6541 agcaaaagaa cggcatcaaa gccaacttca agacccgcca caacatcgaa gacggcggcg
     6601 tgcaactcgc tgatcattat caacaaaata ctccaattgg cgatggccct gtccttttac
     6661 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag
     6721 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac
     6781 tatacaaact cgagatgact ggatccggta ccgaattcag cagggccgac cccaagaaga
     6841 agaggaaggt ggaccccaag aagaagagga aggtggaccc caagaagaag aggaaggtgt
     6901 gactcgagat atctagaccc agctttcttg tacaaagtgg tgatcgaggc aggcccaagc
     6961 attgtgcaca acaaatgctt ttagtaatct ctgcataaag gtgaatagtt ttcctatcac
     7021 cggaattatt tttcttccgt gggcttaccg ttttctgtcc ttgtaaggtt tctgagatcg
     7081 cgttcccata tatatatatc tttttttcaa gtgttgattt actgaaggag cttttaataa
     7141 taatttcaag ccagtcataa tcggaatctg aacattcaag agattttttt tttcaaacct
     7201 tcctccatgt ttgcgtgagc gagctagtcg ataggagttt gcagctttga gctttgatgg
     7261 cttgttggtg gtcgttgact ttttttttgt gtgtgatgct caccctcacg gcggtttgat
     7321 ctttgtccat ttcttgtttt ttcttcttct tttttcacat aacaaacaaa ccagggagaa
     7381 aaactttttt aggttttttg tttatatcgc gttttttttt gtttctaagg atgtctgacg
     7441 atcgaatcag gtgctagccc gctgagcaat aactagcata accccttggg gcctctaaac
     7501 gggtcttgag gggttttttg ctgaaaggag aactatatcc ggatagggct gctaacaaag
     7561 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
     7621 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggggatc
     7681 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7741 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7801 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7861 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7921 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7981 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8041 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8101 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8161 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8221 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8281 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8341 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8401 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8461 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8521 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     8581 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8641 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8701 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8761 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8821 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8881 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8941 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9001 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9061 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9121 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9181 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9241 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9301 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9361 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9421 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9481 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9541 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9601 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9661 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9721 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9781 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9841 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     9901 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     9961 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
    10021 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
    10081 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
    10141 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
    10201 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
    10261 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
    10321 cgagc
//
