LOCUS       sequence-473543-        8726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A piggybac-based vector containing mouse U6 promoter-driven RBBP6 
            sgRNA #6, human U6 promoter-driven RBBP6 sgRNA #8 and CAG 
            promoter-driven nuclear-localized Clover-T2A-BSR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8726)
  AUTHORS   Okubo C, Nakamura M, Sato M, Shichino Y, Mito M, Takashima Y, 
            Iwasaki S, Takahashi K
  TITLE     EIF3D safeguards the homeostasis of key signaling pathways in human 
            primed pluripotency.
  JOURNAL   Sci Adv. 2025 Apr 11;11(15):eadq5484. doi: 10.1126/sciadv.adq5484. 
            Epub 2025 Apr 9.
  PUBMED    40203091
REFERENCE   2  (bases 1 to 8726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-04-11"; volume: "11(15):eadq5484. doi"; pages: " 
            10.1126/sciadv.adq5484. Epub 2025 Apr 9"
COMMENT     Sequence Label: PB-2G-CNCB_sgRBBP6-68
FEATURES             Location/Qualifiers
     source          1..8726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1385..1633
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1385..1405
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1556..1575
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     protein_bind    complement(1967..2000)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2004..2024
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     enhancer        2344..2647
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2649..2924
                     /label=chicken beta-actin promoter
     intron          2925..3941
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3865..3887
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3949..3968
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             4006..4026
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4030..4050
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4054..4074
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(4090..4092,4093..4095,4096..4806)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(4135..4156)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     4743..4764
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4807..4860
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    5296..5417
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5333..5352)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5387..5406
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6180..6214
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6705..6721)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6705..6721)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6718..6740)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6729..6745
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6753..6759,6760..6777,6778..6783))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6798..6819
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6936..6953)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7107..7695)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7187..7206)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7866..8657,8658..8726))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8489..8508
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggcccgat gaggcccttt cgtcttcact
     1381 cgaggagggc ctatttccca tgattccttc atatttgcat atacgataca aggctgttag
     1441 agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg
     1501 tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta
     1561 tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa
     1621 aggacgaaac accggggggc tctcaccgag cgggtttcag agctaagcac aagagtgcat
     1681 agcaagttga aataaggcta gtccgtttac aacttgaaaa agtggcaccc gagtcgggtg
     1741 ctttttttgc tcgaatctac actcagctcg aggatccgac gcgccatctc taggcccgcg
     1801 ccggccccct cgcacggact tgtgggagaa gctcggctac tcccctgccc cggttaattt
     1861 gcatataata tttcctagta actatagagg cttaatgtgc gataaaagac agataatctg
     1921 ttctttttaa tactagctac attttacatg ataggcttgg atttctataa cttcgtatag
     1981 catacattat acgaagttat aaacagcaca aaaggaaact caccctaact gtaaagtaat
     2041 tgtgtgtttt gagactataa gtatcccttg gagaaccacc ttgttggtcg gacgagcgcc
     2101 ccactggttt aagagctatg ctggaaacag catagcaagt ttaaataagg ctagtccgtt
     2161 atcaacttga aaaagtggca ccgagtcggt gctttttttc tcgagtacta ggatccatta
     2221 ggcggccgcg tggataaccg tattaccgcc atgcatcccg ggtgactgac tgagcggccg
     2281 cactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     2341 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     2401 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     2461 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     2521 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     2581 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     2641 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     2701 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
     2761 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga
     2821 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
     2881 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct
     2941 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga
     3001 ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc
     3061 gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc
     3121 gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg
     3181 ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg
     3241 ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt
     3301 gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag
     3361 cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg
     3421 ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg
     3481 tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg
     3541 gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg
     3601 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt
     3661 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg
     3721 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc
     3781 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc
     3841 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     3901 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     3961 ttattgtgct gtctcatcat tttggcaaag aattccgcca ccatgccaaa aaagaagaga
     4021 aaggtagatc caaaaaagaa gagaaaggta gatccaaaaa agaagagaaa ggtacacgtg
     4081 tcaacgcgta tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     4141 gagctggacg gcgacgtaaa cggccacaag ttcagcgtcc gcggcgaggg cgagggcgat
     4201 gccaccaacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     4261 tggcccaccc tcgtgaccac cttcggctac ggcgtggcct gcttcagccg ctaccccgac
     4321 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     4381 accatctctt tcaaggacga cggtacctac aagacccgcg ccgaggtgaa gttcgagggc
     4441 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     4501 ctggggcaca agctggagta caacttcaac agccacaacg tctatatcac ggccgacaag
     4561 cagaagaacg gcatcaaggc taacttcaag atccgccaca acgttgagga cggcagcgtg
     4621 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     4681 gacaaccact acctgagcca tcagtccgcc ctgagcaaag accccaacga gaagcgcgat
     4741 cacatggtcc tgctggagtt cgtgaccgcc gccgggatta cacatggcat ggacgagctg
     4801 tacaaggagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct
     4861 atgaagacct tcaacatctc ccagcaggat ctagaattag tagaagtagc gacagagaag
     4921 attacaatgc tttatgagga taataaacat catgtgggag cggcaattcg tacgaaaaca
     4981 ggagaaatca tttcggcagt acatattgaa gcgtatatag gacgagtaac tgtttgtgca
     5041 gaagccattg cgattggtag tgcagtttcg aatggacaaa aggattttga cacgattgta
     5101 gctgttagac acccttattc tgacgaagta gatagaagta ttcgagtggt aagtccttgt
     5161 ggtatgtgta gggagttgat ttcagactat gcaccagatt gttttgtgtt aatagaaatg
     5221 aatggcaagt tagtcaaaac tacgattgaa gaactcattc cactcaaata tacccgaaat
     5281 taagttaact aactaaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     5341 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5401 ctcatcaatg tatcttatca tgtctggaat tgactcaaat gatgtcaatt agtctatcag
     5461 aagctcatct ggtctccctt ccgggggaca agacatccct gtttaatatt taaacagcag
     5521 tgttcccaaa ctgggttctt atatcccttg ctctggtcaa ccaggttgca gggtttcctg
     5581 tcctcacagg aacgaagtcc ctaaagaaac agtggcagcc aggtttagcc ccggaattga
     5641 ctggattcct tttttagggc ccattggtat ggctttttcc ccgtatcccc ccaggtgtct
     5701 gcaggctcaa agagcagcga gaagcgttca gaggaaagcg atcccgtgcc accttccccg
     5761 tgcccgggct gtccccgcac gctgccggct cggggatgcg gggggagcgc cggaccggag
     5821 cggagccccg ggcggctcgc tgctgccccc tagcggggga gggacgtaat tacatccctg
     5881 ggggctttgg gggggggctg tccctgatat ctataacaag aaaatatata tataataagt
     5941 tatcacgtaa gtagaacatg aaataacaat ataattatcg tatgagttaa atcttaaaag
     6001 tcacgtaaaa gataatcatg cgtcattttg actcacgcgg tcgttatagt tcaaaatcag
     6061 tgacacttac cgcattgaca agcacgcctc acgggagctc caagcggcga ctgagatgtc
     6121 ctaaatgcac agcgacggat tcgcgctatt tagaaagaga gagcaatatt tcaagaatgc
     6181 atgcgtcaat tttacgcaga ctatctttct agggttaatc tagctgcatc aggatcatat
     6241 cgtcgggtct tttttccggc tcagtcatcg cccaagctgg cgctatctgg gcatcgggga
     6301 ggaagaagcc cgtgcctttt cccgcgaggt tgaagcggca tggaaagagt ttgccgagga
     6361 tgactgctgc tgcattgacg ttgagcgaaa acgcacgttt accatgatga ttcgggaagg
     6421 tgtggccatg cacgccttta acggtgaact gttcgttcag gccacctggg ataccagttc
     6481 gtcgcggctt ttccggacac agttccggat ggtcagcccg aagcgcatca gcaacccgaa
     6541 caataccggc gacagccgga actgccgtgc cggtgtgcag attaatgaca gcggtgcggc
     6601 gctgggatat tacgtcagcg aggacgggta tcctggctgg atgccgcaga aatggacatg
     6661 gataccccgt gagttacccg gcgggcgcgc ttggcgtaat catggtcata gctgtttcct
     6721 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6781 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6841 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     6901 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     6961 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7021 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7081 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7141 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7201 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7261 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7321 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7381 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7441 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7501 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     7561 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7621 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7681 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7741 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7801 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7861 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7921 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7981 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8041 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8101 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8161 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8221 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8281 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8341 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8401 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8461 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8521 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8581 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8641 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8701 gcgacacgga aatgttgaat actcat
//
