LOCUS       sequence-473550-        6303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express HER3(1-664)-SmBiT Fusion Protein in Mammalian Cells under a 
            CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Promega
  TITLE     Promega Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: NanoBiT Assay Vector, HER3(1-664)-SmBiT
FEATURES             Location/Qualifiers
     source          1..6303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     501..520
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(606..653)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(668..1025)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      682..817
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        834..1025
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     982..1002
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    1270..1391
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1307..1326)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1361..1380
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1428..1446
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     primer_bind     complement(3640..3659)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3641..3659)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3701..3720)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          3703..3835
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        complement(3971..4174)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4000..4020)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4175..4554
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    4745..5028
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     rep_origin      5144..5732
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5633..5652
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5971..6019
                     /note="synthetic polyadenylation signal"
     CDS             complement(join(6083..6303,1..574))
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6194..6213)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg
       61 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
      121 gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg
      181 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
      241 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
      301 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg
      361 tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt
      421 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag
      481 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg
      541 gagaacctgc gtgcaatcca tcttgttcaa tcatgatctg cctcctgggt ttagttcctc
      601 accttgtcgt attatactat gccgatatac tatgccgatg attaattgtc aacagttaat
      661 taagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
      721 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
      781 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
      841 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg
      901 actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
      961 gggagcctgg ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc
     1021 ctcagaaggt acctaaccaa gttcctcttt cagaggttat ttcaggccat ggtgctgcgc
     1081 agatccgcgt atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     1141 gaaattgtaa gcgttaatat tttgttaaaa ttcgatttta ccacatttgt agaggtttta
     1201 cttgcttaaa aaaacctccc acatctcccc ctgaacctga aacataaaat gaatgcaatt
     1261 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     1321 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     1381 aatgtatctt atcatgtctg ctcgaagcgg ccgccccaag gggttatgct agttattgct
     1441 cagcggtggc agcagccaac tcagcttcct ttcgggcttt gttagcagcc ggatcagctt
     1501 gcatgcctgc aggtcgactc tagagtcgcg gccttacaga atctcctcga acagccggta
     1561 gccggtcaca ccgctcgagc ctccacctcc gctcccgcca ccacctgaac tgccttgaga
     1621 aaccatgaag aggccgatcc ccagggccac caccagcagc aagaggaggg cccccaccat
     1681 cccagtggcg atggacggga tcttaggccc attcgttgga cagccttcaa gacctggccc
     1741 tgtgcatccg taggtgcagt ttggatggca caggtggcac acatggccgg cgtctgcgta
     1801 cttccagacc agggtgttgt tttctcccat gactcctgcc gggcaggtct tgacgcagtg
     1861 ggggccgtca atgtagtggg cacactggat acagttgtct ggtccccgtc ctgtgcaggt
     1921 gatgttcatg gcctgaggca ggcactctgg gtggcactgt atgcactcag agttctccac
     1981 aaactccctt ggctcaccct ccagaaggtt gcacttgtcc acgcattccc tgcctcggct
     2041 gacattccgg caagagacgc agtccttggg ctccgggccc cagcagccct cgggggagca
     2101 caaggcatgg cagacctggc ctgtggcctt gcagctgttt tcacctctgt tgcttataat
     2161 tttggttttc tgaccggagg tcccaaacag ttttttccag tttattgtat ttgcatagca
     2221 caaatttttg tttcctgaaa ttatcacatc tccatcactt atctccttga gggagcgtaa
     2281 tcccaaggat gttatgttca ggctgacgac tgcaagagaa aactgaccat gttgcttggt
     2341 cctgccgcgt atgatttcta ggttctcaaa ggcatggagg tccgtcctgt tttcaggcca
     2401 agcctgaatc agcaaaaacc ctgtgatttc ctttacggtt ttcagaatat ccagttcctg
     2461 tggatccaga ggaggagtat gtgtgaagga gtcaccccta aatgccaccg gcaggatgtg
     2521 gagatcgcca ctgatggagg tgcagttttt gaagtgttta atattcgtag catttatgga
     2581 gagtgagtct ttaaattcac caatacctat tccgttacac actttgcggc aaggcccttc
     2641 gcacttctta cacttgcgga cgccgtcttc ctccatctca tagctgtcgg ccccacaggc
     2701 tcggacgcac gagccgtgat ctgtcaccac ataattacgg ggacacttct tcacgcaggt
     2761 ggcaccaaag ctgtatttgc cctcggggtt cacatccatc tggtacgtgg tggggttgta
     2821 gagcatgagt ggggggcagg tgtccttgca cgtggcttcg tctcggaatt tgcggcagac
     2881 caggcagtcg ctctcccggg ggcctgtgca gcctgcagca cactggttgt ggcagcagtc
     2941 actgggggac ttgccacggc agcgcccgga gcactgctgg gcacagatga ttttggtcag
     3001 tttctggcag ttctcctctc ctgcacccca gcagctccca ttgggacagc ttggatcaca
     3061 cttttggcag ctgcccaggt ggttctggaa gtccatcgac atgttgctga gaaagtcact
     3121 gctgactatg tcccgccact ggatgctctc cacgttgcac agggcagggt tgttgctgaa
     3181 ccgcacggcg ccatgcagga tttcctgtaa atttctcatg ggcagctcct tcagtccggt
     3241 tttatttgca tcatagttag ataagactgc taaggcatag gaattttcgt agtacatatt
     3301 tcctctgatg atctgcaggt tttccaaagg aattcgctcc actgtgttga gggcaatgag
     3361 gacataacca gccacctcct ggatggtctt taagaaggaa agatcataat tcctctgcac
     3421 ataggtaatt tccaaattcc caaggaccac ctcacagtta ttgaacatcc tctggaggct
     3481 gagaaaatga tcttcaaaag tgcccaactg cgtgagcttg ttactcgtgc cttggcaaac
     3541 tttcttttcc tccagagccc gactcgccgg gcagagcgca gccagcagcg ccaggagcgc
     3601 tgccccggcc gtcccggagg gtcgcatggc gatcgctagc cctatagtga gtcgtattaa
     3661 tactctagcc ttaagagctg taattgaact gggagtggac acctgtggag agaaaggcaa
     3721 agtggatgtc agtaagacca ataggtgcct atcagaaacg caagagtctt ctctgtctcg
     3781 acaagcccag tttctattgg tctccttaaa cctgtcttgt aaccttgata cttacctgcc
     3841 cagtgcctca cgaccaactt ctgcagctta agttcgagac tgttgtgtca gaagcactga
     3901 ctgcgttagc aatttaactg tgataaacta ccgcaataaa gcttctagtg atctgacggt
     3961 tcactaaacc agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg
     4021 tcaacggggc ggggttatta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca
     4081 aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc
     4141 cacgcccatt ggtgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt
     4201 agatgtactg ccaagtagga aagtcccgta aggtcatgta ctgggcataa tgccaggcgg
     4261 gccatttacc gtcattgacg tcaatagggg gcggacttgg catatgatac acttgatgta
     4321 ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta
     4381 ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg
     4441 gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat
     4501 gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa tgccaacatg
     4561 gcggtcatat tggacatgag ccaatataaa tgtacatatt atgatataga tacaacgtat
     4621 gcaatggcca atagccaata ttgatttatg ctatataacc aatgaataat atggctaatg
     4681 gccaatattg aagatctcga tcctctacgc cggacgcatc gtggccggag tactgcggtt
     4741 tatctcgatg gctacgaggg cagacagtaa gtggatttac cataatccct taattgtacg
     4801 caccgctaaa acgcgttcag cgcgatcacg gcagcagaca ggtaaaaatg gcaacaaacc
     4861 acccgaaaaa ctgccgcaat cgcgcctgat aaattttaac cgtatgaata cctatgcaac
     4921 cagagggtac aggccacatt acccccactt aatccactga agctgccatt tttcatggtt
     4981 tcaccatccc agcgaagggc catccagcgt gcgttcctgt atttccggac tataaaagga
     5041 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5101 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgaaat cctttttttc
     5161 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5221 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5281 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5341 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5401 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5461 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5521 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5581 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5641 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5701 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5761 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     5821 tggataaccg tattaccgcc tttgagtgag ctgataccgg gtagaaagcc agtccgcaga
     5881 aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg gaaaacgcaa
     5941 gcgcaaagag gatccttatc gctatcgatt cacacaaaaa accaacacac agatgtaatg
     6001 aaaataaaga tattttattg cggccatcgt gatggcaggt tgggcgtcgc ttggtcggtc
     6061 atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc
     6121 gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc
     6181 caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac
     6241 ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca
     6301 agc
//
