LOCUS       sequence-473589-       12314 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express NCOR2-LgBiT Fusion Protein in Mammalian Cells under a CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12314)
  AUTHORS   Promega
  TITLE     Promega Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: NCOR2-LgBiT Vector
FEATURES             Location/Qualifiers
     source          1..12314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(4533..4551)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4534..4551)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     enhancer        4671..4974
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5129..5149
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        5182..5229
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5261..6055
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5315..5334)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5925..5944
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      6272..6817
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     6718..6737
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6934..6951
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7101..7149
                     /note="synthetic polyadenylation signal"
     CDS             complement(7173..8210)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             7186..7194
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(8241..8598)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8255..8390
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        8407..8598
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     8555..8575
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    8670..8751
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     8695..8714
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cagcgggtgc acgggtggga acgtccggcc ggggctgccg atgagggagc gtacgtcgtg
       61 ctttttggag ccagtggtgg acgcgccggt gtcgtacttg agcggggtgc cctgcgtgat
      121 ggagccctcc ttgagcggcc gcggggccag gggcagctcg ggcgtgtgcc gcagctcctc
      181 gcgcgggatc tcatggatgg agcggcccgc ctccttcacc gtggccacca ggccctcatg
      241 ggccggcttc agcttcaggg ggcccagggc ctgcgtcttg taggcctcgg tcaggtcccg
      301 tgagggcggt gggggcggag gcgtgccctc ccgctttagg agcttggcct cccgacgcag
      361 gtagtcctcc tgtgcctcca cgtaggaccg agggatccct tgtgtgatgg acccgcggat
      421 gtggtgctgc tctttgaggt ggtgggggct gtgtcgctcc ggcgggatgg cacggcccat
      481 gagaccttcg atgctggctg aggagatggc tctgcccacg cggccctcca tcatgtcata
      541 ggtgcgcttg ggggcggccg tctcatgggg gggtcctgag ctgcttctgc cgtcctcctt
      601 ggagcactgg gtcacagaca tgccaccctc ataggacaag acgtggccct tcttgccttc
      661 gtagatgacg tggcccttgg gcaggctgtc ctcccggccg cggtccaagc gactcgggct
      721 gtcctcgccg atgatcctgg tgatggtgcc cttgtacagg acgtcagctg gcgtgccgtg
      781 ggtgatggag ccgcggtatg tgatggcgct gtccgagggc acccgtgtgc tgggaatgcc
      841 tttggtgatg cttccgcccg gaactgagcc cagagctgtc cctctcagca cggacgcctc
      901 ctgggctgtg ggcaccccca ggctctccgg tggcccagcc tggccccgtg gggacagctg
      961 ctcctgcttc actccgctga agggtgccag ctttttgggg tccatgggca ggggcagccc
     1021 catggtgaca gggcccaccg gggccttggc atgctctgag tacgggacgt ggagctggac
     1081 cgacattcct tgggagatgg cacctatttg cctctcgagg acgctggggt gcttggcaga
     1141 ggagatgagg ggaggcgggt tggagatggt gggtgggcgc ggcaggacgg gccgggcagt
     1201 gtcatggagg cccaggggca gtgggtgacc aggtggagcg taggagaagg ctgaggggtc
     1261 cggggcatgc ggggaggcct tgatcacctc acgggggggc acggggaagg gcaggccgga
     1321 agtccagcaa ggggggtccc caggcagctt ctgggcctcg gctgcgaagg ctgggaagaa
     1381 cacaggcttc tctgcctcct tgtcggcggg gggtgccggg ctcctgctct tgccccgggg
     1441 gctgctgcca ggctgctgag gggcgtcgct ctccggctgc aggttttgcg gtggcggtgg
     1501 ggctggggga gctggcttgg tgggagctgc gtcctcccgg gggggctcat ggactttggt
     1561 gacctggatg ggggggatgg cagccgctcg ctgcttcagc tgcttcaggt ccagtggctt
     1621 ctggggtgag gcattggccc gggggtcgcc agtcggggtg aggaggctgg gccttgggga
     1681 cagcagccgg ttcttgtcgc cgccctcggc ctcatccacc tcgtctgcac tgcaggtggc
     1741 actggagtcg ctgtcctggg gggcgcccga gctcttggct gtggtggccc tgccgctccc
     1801 gccctccttc ttctctgcct tgagcgcccc ctcggccgtg gcctcagcgg cctccgcgtc
     1861 cttgcccttg gccggcccct cctcggcttc ctccgtgcac tcgctcttga cgggctcctc
     1921 ggccttccct gtgtccactg ccagctcctc agccgcgggg ggcttctgct cctccccctc
     1981 ctccactggg ggcgctgctg cggtctcctc ctccttctcc tccttgggga ccacaggagg
     2041 aggtgcagag ggcgatgggg gtgctggtgg gggcgtaggg gctccggtgg cttcagaggc
     2101 cggggtgggc tcagtggggg ccgggatgtc ctccggtggt ggggtgggtg gctctggggg
     2161 tggcccgtcg gcgcccaggg tggctggggg cttgggccca ttctgccctg tgtccttggc
     2221 ggcctcagtg tgaggagagg ggatgctctc ggtgtctgag ctgttgttga cagtggctgg
     2281 gccactgcat tcccctctgg gcacctcatt cccagaggca tgtaaggctt cagcctcctc
     2341 caccatctcc tcctcatttc cgctcacgcc cgacgcctcc atctcctcat cctccaccac
     2401 gggcgggaat gcagcctcct cgctggccgc cgccggcgct ttcttcttct tcctccgcgc
     2461 gttcctctcc ttctccatct tcagcttgtg ctgctgcaag atctcatcga ggttctgcct
     2521 cttcttgtag ttgaagtaga agttcttaca ctgcgacaca gtcttggagc ccaccatccg
     2581 ggcgatggcc gaccagttgc ggccgtgttc caggagacct ttcttggctg tttccatttc
     2641 ttcttctgtc cagcgagaac tctcattcag ctccatggag gccagctcgg cgctctgctg
     2701 gggggtgatg gcctcctcgc tgttggcctc attagccatt gagcgggtga tgcggccttt
     2761 gcgtcttccc tggctgttgg cagttttgcg gcctttggag gccacagcct ccttctcgtc
     2821 gttgtcctcc cctgaggtgt cgtctgtctt ctccttgagg aggtcttcct tgtcgttctc
     2881 cacctccggc ttctcctcct ccttctccgc ctccttttcc ttctccttct catctttctc
     2941 ctcctggctg ctgcggggca tgggctgctg ctgctgctgc tgctgctgct gctgctgctg
     3001 ttgttgctgc tgctggctct tgccgcggcg ccgatagctc cgtctcacca ggctcttata
     3061 gttctcattc ttcttagtca ggtagtaata gaggacgcac tcagccactg tcttcctctc
     3121 caggaatgat gcgatcaggc caaagttctt gggatgctgc atgaacttct cccggaaggt
     3181 ctccttctcc tgctcactcc acatgttcat gacctggcgg tctttgtaca ccttcatggg
     3241 gtcggccata agcccgttca tgttgatgaa cttgatgcgc tgctggtcag cgtcgtacag
     3301 catgggcggg atcacggcca gctggcgcat ctgcttctcc aggttctcct gctctgagag
     3361 gccatcgatg atctctgaca cctcgtgctc gctgcgggcg gccgacatgg acagcccact
     3421 gccccgctgg cccaccctgc tctgcatgcg ctcctgcagc tcgcgctgct tgcggatctc
     3481 agggaactgc ttctcgtagt actcgcgcac cttgctctcc ttggcccgcc gccgggggtt
     3541 gttctcgatg cgctccacct tcttctccca ggcctccatg agctggtcat agcgctggca
     3601 gaacttctgc tcccattgtt tccgagcgtg attcctcctc ttgaagtaca agattagctt
     3661 cttccgcatc gcctggttta ttttgatgtt ctcatgatac tgccgggtgt cggagggctg
     3721 gttgtacagc ggcagctcca cctggggccc caggccttcc agaatccgat gtgcagcttc
     3781 agccttcttc cggttctcgt cgtagatgat ctgcaccagg ctgcggtgct tcgactcgat
     3841 gggcggcggt gacacgggct tctcaggctc gggcggcttg gcagcctcct cctccagctg
     3901 ttgctgcttc ttcttcagct tagagatctg ctgctctacc atggtgatct ctcggtccac
     3961 gcggtccatg ttctggatca gctcctcctt ggacagccgt ggcggcacca gctccagctc
     4021 agggtcagtg tgcggggggc tggggggaga caccggttcc agcttgcccg tcaggctacg
     4081 gtccttggtg aggtcttcag atcccgcagg ctggcccgtg gccagcaggg gtgacggtcg
     4141 cagcaggggg tcaggcagca gctctagccg agggcgcttg ctttcaatga actccatctc
     4201 tgacttcccc agctcgggca ggtatgagtg ggactctggc cgcaggtgga gctcctggga
     4261 ccgttcattc ccgggctgga actcagacag cagggagggc ctccgccgct ggggctggat
     4321 gatggagccg ggcgacaggt gggaggcata gtcgcgggag tggtgctggt actccaggag
     4381 cccgacgtcc gtgtgcgtcc gggcgatctg cactgggtag gaaaggctgt ggggcgggta
     4441 gcggggctca gtggccctcc acgtctgtgc cacaggctgt gtggatcccg acatggcgat
     4501 cgctagctgc aaaaagaaca agtagcttgt attctatagt gtcacctaaa tgggcgatct
     4561 gacggttcac taaaccagct ctgcttatat aggcctccct ggcgcgccac tagttattaa
     4621 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     4681 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     4741 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     4801 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtccgccc
     4861 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     4921 cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     4981 cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     5041 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     5101 aaatgtcgta ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt acggtgggag
     5161 gtcaaatcga attctaatac tgttgacaat taatcatcgg catagtatat cggcatagta
     5221 taatacgaca aggtgaggaa ctaaacccag gaggcagatc atgattgaac aagatggatt
     5281 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     5341 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     5401 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     5461 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     5521 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     5581 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     5641 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     5701 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     5761 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     5821 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     5881 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     5941 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     6001 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgattcga
     6061 aatgaccgac caagcgacgc ccaaccggta tcagctcact caaaggcggt aatacggtta
     6121 tccacagaat caggggataa cgcaggaaag aacatgtcaa acatgagaat tacaacttat
     6181 atcgtatggg gctgacttca ggtgctacat ttgaagagat aaattgcact gaaatctaga
     6241 aatattttat ctgattaata agatgatctt cttgagatcg ttttggtctg cgcgtaatct
     6301 cttgctctga aaacgaaaaa accgccttgc agggcggttt ttcgaaggtt ctctgagcta
     6361 ccaactcttt gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa cttgtccttt
     6421 cagtttagcc ttaaccggcg catgacttca agactaactc ctctaaatca attaccagtg
     6481 gctgctgcca gtggtgcttt tgcatgtctt tccgggttgg actcaagacg atagttaccg
     6541 gataaggcgc agcggtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga
     6601 actgcctacc cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg
     6661 aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc cgccaggggg
     6721 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcagat
     6781 ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggctttgccg cggccctctc
     6841 acttccctgt taagtatctt cctggcatct tccaggaaat ctccgccccg ttcgtaagcc
     6901 atttccgctc gccgcagtcg aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa
     6961 tatatcctgt atcacatatt ctgctgacgc accggtgcag ccttttttct cctgccacat
     7021 gaagcacttc actgacaccc tcatcagtgc caacatagta agccagtata cactccgcta
     7081 gcgctgatgt ccggaatcgt cacacaaaaa accaacacac agatgtaatg aaaataaaga
     7141 tattttattg cggccatcgt gatggctaga gtctaaactc gacctacctc cttagcgcgc
     7201 ggtcgtgtac tgggccggcg gttgccgctg tcggccagca cctcgacgca gccgtcggtc
     7261 catacggctg cgctccggcg agcgatttgt gtgcgaccga cggtgccggc cccgctgcgg
     7321 acgatggcat cgcagcggcc ttgagcccag gcagcatcgt cgaagttgcc gtccacgagg
     7381 ctctggtaga gctgatccag gccgatgcgc agcatgtagg ctcgcagacg agggctgccg
     7441 gccagctcgg gatgccggcg ctcgaagtag cgagtctgct gctccatgca agccagccag
     7501 ggccgccaga agaagatgtt ggccacctcg tactgactgt ccccgaacat agcttcggac
     7561 cagtcgatta cggcggtgat gcggccgttg tcggtcagga cgttgttgct gccgaagtcg
     7621 gcatggacga ggtggcgcac ctcgggacag tcttcggccc acagcatcag ttcgtccagg
     7681 gcttgagcta cgctggcgga cacggtgtcg tccatcacgg tctgccagtg gtagacatgg
     7741 ggatcagcaa tggcgcaaat gaaatcccgc caggttgtgt actggccgat gccttggggc
     7801 ccgaatgggc cgaagccgct ggtttgactg aggtcggcgg cggcaatagc atccatagct
     7861 tcggcgacag gctgtaacac agcaggcagc tctgtttcgg ggaggtcttg gagagtaacg
     7921 ccttgggcgc gtctactgat gcagtatgtc aggctctcgc tgaactcgcc gatgtccaac
     7981 acttcgggga tgggtagtgc agcgctggcg aagtggcggt acacgtagcg gtctttgtag
     8041 aagccatcag cgcagctatt cacccgcagt acatagccgc gtccgccgac atcgaagctg
     8101 aaggctcggc tctcttcgcc ctccgacaac tgcatcaggt cgctcacact gtcgaacttc
     8161 tcgatgagaa atttttcaac gctggtagcg gtgagttcgg gcttcttcat ggtggcgcta
     8221 gtgtcagaag aatcgagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac
     8281 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt
     8341 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt
     8401 aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     8461 ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata
     8521 cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac attccacagc
     8581 tggttctttc cgcctcagaa ggtacctaac caagttcctc tttcagaggt tatttcaggc
     8641 catggtgctg cgcaagacgc gtgacgtcgg cagtgaaaaa aatgctttat ttgtgaaatt
     8701 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     8761 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     8821 taaaacctct acaaatgtgg tatggctgat tatgatccgc ggccgcaata cgtcgacgtt
     8881 atcagctgac ttcgtacgag agcctaggtg tttctagagt cgcggcctta gctgttgatg
     8941 gttactcgga acagcatgga gccgtcgggg gtgatcaggc gctcgtcgat aattttgttg
     9001 ccgttccaca gggtccctgt tacagtgatc tttttgccgt cgaacacggc gatgccttca
     9061 tacggccgtc cgaaatagtt cagcatgttc ggcgtaaccc cgtcgattac cagtgtgcca
     9121 tagggcagga tcaccttaaa gtgatgatca tccacagggt acaccacctt aaacacctct
     9181 tcgatctggg ccatttggtc ggcgctcaga ccttcatacg ggatgatgac atggatgtcg
     9241 atcttcaggg cattttcacc gctccggaca atcctttgga tcggagttac ggacacggcg
     9301 agattctgca gcaaactgga cacacctccc tgttcaagga cttggtccag gttgtaggcg
     9361 gctgtctgtt cccagtcccc aacgaaatct tcgagtgtga agacaccgct cgagcctcca
     9421 cctccgctcc cgccaccacc tgaactgcct tgagaaacct cgctgtcgga gagtgtctcg
     9481 tactgcgagc agagcagtgg cttgggctcc tcgtcccagg cgtggtgggg gccagcgagg
     9541 ggcccgctgc ccgcggggag gcccggtggg ggtggggaag ccatgacacc cgcctgcagc
     9601 cgcatgatca gggggttgta ggggaatggc gtggaacctg cggacgaggg cctgtcctcc
     9661 cacacgcggt tggtgagcgg cgtccggcgg ttgcagtctc cctccgagtg cactgaggag
     9721 acagagggtg gccggtcccc agatgccagg cccggggccg gggacttggc ttttcggctg
     9781 ctgggtctgc cagagacctt ggccttcccg ccgccacctg ttccggtgat ggcgggcata
     9841 ttgaagatct ccgtcccagg ctggctgata ttgtattcag gctcattccg gttgtgggtg
     9901 ttcagcttct tgttgatctc ttgcttcttg gacttgacca tggcggagtt gctctcggtc
     9961 agcttgctga agaaggctgg cggctggctg gtgttgcctg gagacttgga gcccatcctg
    10021 ctgggctccg tctgttcccc atcccggtac agcagcgggt acacagcact ccgggagtgc
    10081 cctggctccg tcatgccctc cggtggggac acaggttcaa taccgtcctc accaccaccc
    10141 aagaccgacg tcttgtttgg ctctggagac ctcttgcccc cttcgctgtg gggggagcca
    10201 cgggccgggg caccatggtc cgggggcggg aggtagaggt cactgggtgg gcggcggagg
    10261 tccaggacgg ggcagctggc cccagggaag gagtagaggg gggcgggcag gggtgcgctg
    10321 agctgctgtg ggtggtgccg ggtgtagtcc tgtgtgatga cctcactgat gtgctgggcc
    10381 agggtgacca cccgctggtg acctttgacc cctggggcgg tctggagcag cgggctggac
    10441 gagggctggc tctcaggcag cggccgcagg tgtgggaggt gggcggcctc cccgccaagc
    10501 ttcacggggc ctggctgctt gggccgcagc tccccctcca ggtggctctt gtcgagctct
    10561 tccaggtgct tggggagccc cttgtcgtgg gtcagactgg gtgagctcac agggctgacg
    10621 ggctccaccc cttcggggct gtagctgctg ccgtggtaac ccagagaacg gagttccagt
    10681 tcctggatgg aaaagggttt actttgagtc ttttcccggt gcgggtccga ggccgaggca
    10741 ggtggcgccg gcgggtccgg gctggcgtgg tgaggtgcga ggttcttcgc aggggtgcgg
    10801 gcgatggtgg cgtggccaga gacaggaggc actaggggcc ggggctccga gcccttgctg
    10861 ggggaggagg cgggctccag cccggagcgg gctgggggct tggcgaggaa ggcatggccg
    10921 gtgtctgctc ggggccgctc tggccgggcg acccgggggg cctccttggg cagcaagacg
    10981 ggctccatga gggtagggta gaccccatcg agggtgccgc ccagtgggca gtgggtggca
    11041 ggtgggaatg tggcagccgg gcgaacgggt gaggaggtgg aggtggacct caggaccgtg
    11101 ggcgtgctgg gctccacagc ggtgatgata cccttcatgc ctgtgttgtg aagcacactg
    11161 ggtctctgct ggagggcatc ctgggtccga ggggagatgg gcgagtgctg gtgggcatgg
    11221 gagtgggagg cggggcggct gctgctgccc ccacccccgc cgctgctgcc gctgctgccg
    11281 ctgctctgct ctgtaccagg tctccagatg ggtgcgtgct ccaccgtcgt ggtggacgtg
    11341 aggatggact tttcccgctc ccgatcccgg tcccgctctc gatcccggtc tcgctcccgc
    11401 tcggacgagg acgtggtggt tggttttgtc aagtgtgttg gacctcctgg ggagagtggg
    11461 gagctgctgt ggcggctgct gaagggctgg ggcgcggtgg ggaggtaggc aaggcggtcc
    11521 atggcggtgg ctggggtgcc tggtgtcggg ggcacgagca caggcaggtg tggcacttgg
    11581 gacaggtcga tgatgcctcg gggacccgca gcgtagttga gtgccagcga ggactcgcgg
    11641 ggcgagaggc ccctcagcat atcagctcgc tgggccatgg cggtggccgc gttgtggtgc
    11701 atctgctgcg aggtgatgta gtcattgatg atggtctgcc ggttctccag cgccgccgtg
    11761 tcggggtagc cgcggatgag gtagggtggg tacaggtgcg ggtaggtggg gttgggggcc
    11821 aggtgtcggg gcaggtagta ggcagcggct gcgtccagag ggatgccgcg gggtatggag
    11881 gtggggtcga aggccagggg gatgtggctg cgatacaggt ccacgccact cacgccccga
    11941 agcaggtgct catagggcga gatggggtgt gggtggtgct cgggcacggt gctgtgcggg
    12001 gacttggcga tctcacgagg cgtcgacgtc agctttcggt cctgggatgc cttgctggac
    12061 gaaaggctgc cctcctgcag gcgcggcgtg ggctcccgcg tggtcacggg cgaacctcgt
    12121 gggaggtggc cggcaaaggg tgccccgtgg tcctcatagg ttagggggct ctgccgcggc
    12181 ttgcccagct caggcacaat gaccggggcg ccgcgcgcaa tggagccccc cgagctgctg
    12241 gcggtccctg gccggctctt caggctctcc tcgtagcagg cacgttccag tgcccgggcg
    12301 tcggccatca catc
//
