LOCUS       sequence-473596-        4817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE2664m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 4817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP20169 - pAAV-AiE2664m-minBG-iCre(R297T)-BGHpA
FEATURES             Location/Qualifiers
     source          1..4817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      753..762
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             765..785
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1851..1962
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2080..2220
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2080..2209
                     /label=AAV2 ITR
     rep_origin      2295..2750
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2382..2401)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2592..2613
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2767..2786)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2886..2908
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2946..2964)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3032..3136
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3137..3205,3206..3997)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3355..3374)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4168..4756
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4657..4676
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaaggaa tgtgaattag attgtttgac aactttaaat
      181 atcctctctc tctctctctc tctctctctc tctctctctc tctctctctc tctctctctc
      241 tctctctctc tctctctctc tccataaaag ggcaatagta ctgacaggtg tcccagagga
      301 gtctctgtgg gaattaacat caacagcaaa cagagtacag gtgcttacaa tgcccctggc
      361 ttttggagaa gtactgaatg aggccagtaa cttgcacaga atggtgggat gtgcctgaac
      421 cacagacaag cagatggcag agacagaagt gaaccaggca gtctgcttta aaaatatata
      481 aagtagctgg gtatgcaatc ccagaattta ggaaacggag acatatgtat tagggattca
      541 aggccatctt tagctacaca ggccgatctg aactatgtga gaacatgtct tgaaaacaac
      601 aagaggtttt agactttaaa atgtacttag tcaatatggt gtctgacata tggaaggcat
      661 tcacgccggc ggagctcggg ctgggcataa aagtcagggc agagccatct attgcttaca
      721 tttgcttctg ggatccagat ctttcgaagc tagccaccat ggtgcccaag aagaagagga
      781 aagtctccaa cctgctgact gtgcaccaaa acctgcctgc cctccctgtg gatgccacct
      841 ctgatgaagt caggaagaac ctgatggaca tgttcaggga caggcaggcc ttctctgaac
      901 acacctggaa gatgctcctg tctgtgtgca gatcctgggc tgcctggtgc aagctgaaca
      961 acaggaaatg gttccctgct gaacctgagg atgtgaggga ctacctcctg tacctgcaag
     1021 ccagaggcct ggctgtgaag accatccaac agcacctggg ccagctcaac atgctgcaca
     1081 ggagatctgg cctgcctcgc ccttctgact ccaatgctgt gtccctggtg atgaggagaa
     1141 tcagaaagga gaatgtggat gctggggaga gagccaagca ggccctggcc tttgaacgca
     1201 ctgactttga ccaagtcaga tccctgatgg agaactctga cagatgccag gacatcagga
     1261 acctggcctt cctgggcatt gcctacaaca ccctgctgcg cattgccgaa attgccagaa
     1321 tcagagtgaa ggacatctcc cgcaccgatg gtgggagaat gctgatccac attggcagga
     1381 ccaagaccct ggtgtccaca gctggtgtgg agaaggccct gtccctgggg gttaccaagc
     1441 tggtggagag atggatctct gtgtctggtg tggctgatga ccccaacaac tacctgttct
     1501 gccgggtcag aaagaatggt gtggctgccc cttctgccac ctcccaactg tccacccggg
     1561 ccctggaagg gatctttgag gccacccacc gcctgatcta tggtgccaag gatgactctg
     1621 ggcagagata cctggcctgg tctggccact ctgccagagt gggtgctgcc accgacatgg
     1681 ccagggctgg tgtgtccatc cctgaaatca tgcaggctgg tggctggacc aatgtgaaca
     1741 ttgtgatgaa ctacatcaga aacctggact ctgagactgg ggccatggtg aggctgctcg
     1801 aagatgggga ctgaggcgcg ccgaattcaa gcttctcgag agatcttcga ctgtgccttc
     1861 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     1921 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     1981 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     2041 tagcaggcat gagatctcac gtgcggaccg agcggccgca ggaaccccta gtgatggagt
     2101 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
     2161 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg
     2221 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
     2281 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     2341 gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc
     2401 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     2461 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
     2521 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     2581 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta
     2641 ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat
     2701 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
     2761 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     2821 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     2881 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     2941 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     3001 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     3061 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3121 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     3181 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     3241 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     3301 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     3361 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     3421 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     3481 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     3541 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     3601 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     3661 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     3721 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     3781 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     3841 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     3901 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     3961 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     4021 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     4081 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4141 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4201 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4261 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     4321 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4381 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4501 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4561 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4621 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4741 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4801 gccttttgct cacatgt
//
