LOCUS       sequence-47360-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   White MD, Milne RV, Nolan MF
  TITLE     A Molecular Toolbox for Rapid Generation of Viral Vectors to Up- or 
            Down-Regulate Neuronal Gene Expression in vivo.
  JOURNAL   Front Mol Neurosci. 2011;4:8. Epub 2011 Jul 4.
  PUBMED    21772812
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Neurosci."; date: "2011"; volume: "4"; pages: "8. Epub 2011 Jul 4"
COMMENT     Sequence Label: pENTR-L3-oPRE-L2
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..94
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(678..777)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(807..826)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(808..826)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(831..847)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(831..847)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 cgcatagatt tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc
       61 ttttttataa tgccaacttt gtataataaa gttggagcat cttaccgcca tttataccca
      121 tatttgttct gtttttcttg atttgggtat acatttaaat gttaataaaa caaaatggtg
      181 gggcaatcat ttacattttt agggatatgt aattactagt tcaggtgtat tgccacaaga
      241 caaacatgtt aagaaacttt cccgttattt acgctctgtt cctgttaatc aacctctgga
      301 ttacaaaatt tgtgaaagat tgactgatat tcttaactat gttgctcctt ttacgctgtg
      361 tggatatgct gctttatagc ctctgtatct agctattgct tcccgtacgg ctttcgtttt
      421 ctcctccttg tataaatcct ggttgctgtc tcttttagag gagttgtggc ccgttgtccg
      481 tcaacgtggc gtggtgtgct ctgtgtttgc tgacgcaacc cccactggct ggggcattgc
      541 caccacctgt caactccttt ctgggacttt cgctttcccc ctcccgatcg ccacggcaga
      601 actcatcgcc gcctgccttg cccgctgctg gacaggggct aggttgctgg gcactgataa
      661 ttccgtggtg ttgtcgtacc cagctttctt gtacaaagtt ggcattataa gaaagcattg
      721 cttatcaatt tgttgcaacg aacaggtccc tatcagtcaa aataaaatca ttatttgcca
      781 tccagctgca gggcggccgc gatatcccct atagtgagtc gtattacatg gtcatagctg
      841 tttcctggca gctctgggcc
//
