LOCUS       sequence-473604-       13388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transient overexpression of IP3R3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13388)
  AUTHORS   Kuchay S, Giorgi C, Simoneschi D, Pagan J, Missiroli S, Saraf A, 
            Florens L, Washburn MP, Collazo-Lorduy A, Castillo-Martin M, 
            Cordon-Cardo C, Sebti SM, Pinton P, Pagano M
  TITLE     PTEN counteracts FBXL2 to promote IP3R3- and Ca(2+)-mediated 
            apoptosis limiting tumour growth.
  JOURNAL   Nature. 2017 Jun 22;546(7659):554-558. doi: 10.1038/nature22965. 
            Epub 2017 Jun 14.
  PUBMED    28614300
REFERENCE   2  (bases 1 to 13388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature22965"; journalName: "Nature"; date: "2017-06-22- 
            22"; volume: "546"; issue: "7659"; pages: "554-558"
COMMENT     Sequence Label: pEGFPc1 QFR IP3R3
FEATURES             Location/Qualifiers
     source          1..13388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      629..638
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(5233..5943,5944..5946,5947..5949))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5233..5943,5944..5946,5947..5949))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5275..5296)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5624..5643
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5883..5904
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5946..5955
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5994..6197)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6023..6043)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6198..6501
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(6676..7264)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6756..6775)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7593..7640
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     7665..7684
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(7872..8666)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(7872..8666)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7983..8002)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8593..8612
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(8701..9058)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8715..8850
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        8867..9058
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     9015..9035
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(9060..9164)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9191..9646
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9278..9297)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9488..9509
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    9653..9774
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9664..9683)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9718..9737
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ggtgatgaga tcacacacca acttggtggc cccctccttg tccagccggc actgggtggc
       61 tgcgatggcg gaccagtcct gatccacgcc agagcctgtg gggtcagtga gctcgccccg
      121 gggaccggac ttccggttct ggaggtaatt ctgcagcagc atcttcctta gctggttgcc
      181 ccggtcccca tacttgctct tcttctgcag catctgctgc agcgtccgca gcaccttcac
      241 gcacagcttc tcctccgact ccatgagacc cttggtgtga cggatgagct tggacaggaa
      301 gccgccactc tcgcagcgct ggtaggcctc actgccctct agaaagagca gctcgggcca
      361 gtgcagcatg tccaccagca ccgagagctc ggcctgcacc agaggcttca gccgctcttc
      421 tagggccgtg atgatgtcct gtaacttctc aatgatgttc ttgtagtccc actggttggc
      481 ggtggggatg acccgaggga aggtcctcgt ggccgtcttg tagttggcag cgctgcgttg
      541 ggccgcggct gagcagctgc ccccactgct gagcagagcg ctcatgtggg catccagatc
      601 catcggcagc aagatggctc tactcttggc caccatggcg agggtgcgga cacaggcctc
      661 caccgagccc ttgtgctgct gctgcagcca aggacactcg agcagccggg tggtggactg
      721 cagcagctga accacaattg tctgatgcgt ctgtagggat gtgctgttct ctgaaaatgg
      781 ggaactgaag aaggcgctga tggtgtccag caccacggtg agcacgtact tctcaagggt
      841 ggggtctgac aggcgcttct cccgcttgtt acagaccaga gccatgtcca gggtgaagtt
      901 ctcgaagagc gtccagatgt ggttgctggt atagatctcc ttcatctcca cctccgtgtc
      961 tacatagcag tggttcacga agttcacata cgccatttta acctccgtga tgcagtcctc
     1021 gtgggtcacc accgacacca cgtcctctag aggcagcagg gaagtgcact tgatctctgt
     1081 gtagacattt ttgccttctg cacaggcagc cagcaagtcc accagggaga tgtggtacat
     1141 gagggggctg tggtcctcca cgccatctcg ggctgccttc atcatgtcca gcagatgggc
     1201 cagagagtcc ttgtcattgt agaacaccac cacgtcgtcg cctgcgttgg tcagctcggt
     1261 catgatcatg tcctgacact tcttcacgta cttgccctcg gccttgatga cggtatgcag
     1321 gaagtccagg tactgcacgt ggcgcccgtg tgtggccagc aggtgcacga agtgttgcag
     1381 caccggctcg ctgatctcag agcacagctg atagttgttg aggaaaatgt gctgcatggt
     1441 ctcagcctcc aggagcccgg gcgtgaggaa gagctgcaag tgcttgtgca gaagggcctg
     1501 gttgccaggg ttcccggcac agaacttctg taggaactgg tgtgtgtagc gcaggatctc
     1561 catcatcttg ttgtcgttct tgtcataagg gatctgcagc aggtccagca tgaccttgtg
     1621 ggcgtccatg ttcttcagca gtctctgttg cttctttcgc atctgctccc cgaccccaca
     1681 catcttgttc agcctctcca ggatgccttt gacaatctgg tagttctcac tgctcttctc
     1741 cccgtgtggc tgcagaaatc cctcctcatc cgagggccgc tccttcttat ccttgctggc
     1801 gcccgcctcc ccctcctcgc ccttgacgct gcctttcttg tccacccaca gctccgactt
     1861 ctccaccatc gtccgcagtc gatccagttc cgacttgatc actttgtagt tctccacatc
     1921 ctgggctgag atgagcagct gcacctgctt gaaggtgtgc atggcctcct ggcgctggct
     1981 gaaatgtttg aagagcagct gcagggcgcc ggagaccaga ggtgggtagt cgtgcatggt
     2041 gaggtgcagc agcactcgca ggaacatgcg acccccctcg tcatccacct ctagcatgct
     2101 gctggtcttc cctactccaa acatggcctc tgcctgctcc ccgatgcggt ccagattcat
     2161 gttggcagta gaggaatcga aggcgggggc tgtgccgtct gccccgctgt cctgcatggg
     2221 aaagacctcc acaaactcct tcttgaagac cgacagcagg taggagatgc ggtagtctag
     2281 gcgcacgttg aggatgaact gcaggatctc caggatcttc agcttggtct ccatcaccac
     2341 ggtgtgttca ttgtcctcaa atttgcttct gtccagctgc tcggggacac ccactccagt
     2401 aggcaggctg gaggcaccaa atacagactg cttgcggctt agcaccatgg tggacatcat
     2461 gtgtcccacc ccctggatgg acctccgcac gttcttgccg ccaggctcct cataggcctg
     2521 cagcacagcg gctggtgcct gaatgcagtc gatgatgccc agcaacgtgc gtgtgagtcg
     2581 cagcagctcg ctgaagctgt agaacccgaa gtagatgagg ttgtgtgcca ggctgaccac
     2641 ctcaaaggtc agtatgttct tctcaacgtt ggcaaagggc acggcctcgc cgaccacgtt
     2701 gttaaggtaa tcctccacga actccatggt gctggcaaac ttgtttttct tgtcatctcg
     2761 ggaggcgttg agattggaat catagtcttt gatggtgatg gccgtgggga tctctgtcca
     2821 gaggcgggca aacttgacag gggtcaccag ctcctggggg tcacggtcca cgtgcacatg
     2881 tagcatcagg tggcagaatg aggcgcggag gtcaaagggc agcatctcgt ccgccatgca
     2941 taggaaaagc agctccacac ccagttgctt ggagatctcg tcgatggcca ggtactggcg
     3001 gtctaggcac atgcgggcaa acagcttcaa ctggtacctg tagtagctga gcacgttctc
     3061 gtcgtgtgcg ttgccggccc ttgcctcctg ggccagctgc ctcacactct tctcgtgatg
     3121 ctcgttgttc ctgtccgtcc atgtgagcca cacctcctcc tccgagtact cgatgctgag
     3181 gtactcgtga gactgagcca tctccttcac gggccgaagc tcagtctgga taaggatgtc
     3241 actgttcttg gggtccagca cacacttgca aatcagctcc tgggtgacgg ggatggcaat
     3301 gcggttggac acgcacaggt cggagaggta gtccaggaac ctgggctccc tgttcttgcg
     3361 cacgaggctc acgaaggttt ccacctcggt cttggtgatg tgcttctcca gcagcttccg
     3421 gttgttgtgc agcagggccg tgatggtgtc ttcggccagg atgtcatagc caatctgtga
     3481 ctgcatcatc ccgaactgct tggctatgtg ctcctggttc ttacggtagt cctcctggga
     3541 gtgccgcagc acccggtaac acagggcggc catgtacgcg tagggggcgt tcttctggtc
     3601 agacagttcc tccagacgca ccaatggccc ttcacccccc ttgtcacgga atggagcctt
     3661 caggatgcca aagatctgct taagtatgtt ctgctcccgc atcagcttct gccgctctcg
     3721 gttgggtttg gtgaccatga tgtccaggac attctgccca ttgttgggaa catcgctgac
     3781 aaaaaacacc aagtcctcca acagctggat gacaaacctg cggtcgttct ggctgatgaa
     3841 gccctcgttg agtttctcca cagcgctggc cagcatggag cttgcatcgt tggcaaagtc
     3901 caggtcgcgg atctcagaca cgggaaccga cacaatggca aaggcctcct tgtcctcctt
     3961 ggtggggcac gtgcccaaca tgagccggat aggccgttcc tcctccacgt cgatgggcgc
     4021 gttcgtgctc tggatccagg tgttggtaca gaggtgccgc agccgcacgt aggagttccg
     4081 gggcacgaag gagtcggttt tctgcagcgt ggtggggtcc agctcgaaga gagacgcgat
     4141 gtcgttgcca tgtggcacag ccaccagacg gtacttgatc ttctcgccgg cgtttctgcg
     4201 gcctgtgcgg ctctgggccc ctggtcctgc tgccttagga tctgagacat cacccttgta
     4261 gctggggttt tcctcagccg ccagatagtt gcccgtggcc aggtgcttga acctgtacag
     4321 accattccag tgtcccgctc cgccgcggca ggggtcatgg tggaccacct ccacctccca
     4381 gagcgcgttg gagctggtgg ccgaggtggc ggactgacgc agcgtggtcc tcaggaacac
     4441 ctgaagcttg ccccggtact cgtcacaggt caggaacttc tcctgctctg catggaacag
     4501 ccgcaccacg tctcccccct tcaacacctc ctccagatgg tcccggaact gcatgaataa
     4561 gttgatcttc cagctggtgt tgcagttgac agagttgacc tccttgcagc caacgttatc
     4621 actaagctca taattgctgg cgtgtagagg ctgccctgcg ttgacagggt tcaggatcac
     4681 cttgtccccc acaaccacgt tatccccatt gctccgaagc ttccagaagg gctggatgaa
     4741 gagccaggag ccctcgttgc ccgtcgcgtc cagcgtcacc cgcatggcgt tcttctccag
     4801 cagggccggc tgccgcttgt tcaccgttaa gtatttgttg ctcttcatgt gcagaagctg
     4861 aatcacgctg ccgtacttca ccacgtcccc gtgcaccttc ttgttctctg tgtcgttttg
     4921 cttctgctcc atctgggctg catgctgcag cttctgcagc aacaccacat cggcgatctt
     4981 ctctttgtcc tgcttagtct gcttggcttt ccagtactgc ttctgtgcgg agtagcggtt
     5041 catggggcac actttgaaga ggcaatctcg gaacttcttg ggggggttgt ccaggtcccc
     5101 agctgcaggc tccaccacac aacggtcatc caccagcccc aaagtgctga tgaagccgtt
     5161 gacagagccc tcggcgtaca gggacacaat gtccccgatg tgaagaaagc tggacatttc
     5221 attcattccg gacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
     5281 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt
     5341 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
     5401 gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac
     5461 cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta
     5521 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
     5581 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
     5641 gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa
     5701 gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt
     5761 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt
     5821 cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt
     5881 tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt
     5941 gctcaccatg gtggcgaccg gtagcgctag cggatctgac ggttcactaa accagctctg
     6001 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
     6061 ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca
     6121 atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac
     6181 tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
     6241 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     6301 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
     6361 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
     6421 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
     6481 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
     6541 attgattact attaataact aatgcatggc ggtaatacgg ttatccacag aatcagggga
     6601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7081 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7201 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7261 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7321 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7381 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa cctgaggcta tggcagggcc
     7441 tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg
     7501 ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga
     7561 gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt
     7621 attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt
     7681 ttcagttagc ctccccctag ggtgggcgaa gaactccagc atgagatccc cgcgctggag
     7741 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc
     7801 ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc
     7861 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     7921 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     7981 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca
     8041 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     8101 ccatgggtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt
     8161 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
     8221 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     8281 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     8341 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
     8401 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
     8461 cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg caccggacag gtcggtcttg
     8521 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
     8581 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     8641 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcgatc
     8701 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc
     8761 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg
     8821 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc
     8881 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     8941 acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
     9001 tggggacttt ccacacccta actgacacac attccacagc tggttctttc cgcctcagga
     9061 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     9121 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     9181 gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     9241 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     9301 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     9361 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     9421 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     9481 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     9541 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     9601 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttacgc gttaagatac
     9661 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     9721 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     9781 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     9841 aagtaaaacc tctacaaatg tggtatggct gattatgatc agttatctag atccggtgga
     9901 tcccgggccc gcggtaccgt cgactgcaga ttcgcggccg ctttttttgg agtctattaa
     9961 tttatttaag gcaattaaca cattagtctc caggacacag gacatgtaaa acattacacc
    10021 aaaaagagag aagccactgt cacacagccc aggcctgttg ctgggcttgt gaggcttgcc
    10081 tacaaagcct agagaaagtc ctgggagcaa ggtccaccct gagtcatgag gctcctggga
    10141 cggggccaca ctgtccccag caggcacagg agggtgggat ggcccccagg aataagttaa
    10201 atatcaaagc cctaggaagc taataacagc aggctaccac cttcctgagc cagggtggca
    10261 ggcaggggac agccacaatg tagtgcttgg aggtgagagg cagcccactt ttaaccacct
    10321 gccagggcca ctgaggaatg ccagtcactg cttcgaagga cagcagtctg gaggacttgg
    10381 catcccctaa ggcactaagg taaaccgagt ctcttcaagg gtacttacta agcaggaccg
    10441 tgctctgctc tggcactccc gctggccaga gcctggcttg gggagggagg gtggcagctg
    10501 agctgaagat ggggcaggca cctggcttct acctgacctg aaggaagggc ggcgctgtgg
    10561 gcagccggcc tcccgtgggg gcctccgagg ctccgctctg ctcagcggct catgcagttc
    10621 tgcacgtcca cgaagcccag acgttgcctc cgcttccgct gctccgtcat ctgttccttg
    10681 agctcattga gctgggcggt gaggtgcgag accagcttca tggtggagcc aagcttctcc
    10741 tgcaggatgc ggatctcgtt ctgctcgccc tcgccctcgc cgctcaccaa ggacatggcg
    10801 cgcatccgtg ggaaccagtc caggttcttg ttcttgatca tctgagccac gtagctctcg
    10861 gggcccgtgt agtctgtctt gttcttgaca cggaccagca caatgaagta caggtagttc
    10921 cacatgttgt gctccagttt gatgtgctct tcaaaggaca ctgtcttgtt gtcaaacttg
    10981 tccctctcca gaccacagat gaagcacgtg gtcttgagga tttcctcttt cttctgcttc
    11041 tcgctgcgca ggtcggcgaa ggtgtcgatg atcaccccaa agatgaggtt caggacaata
    11101 atgatgacaa tgaagaagaa caggaggtcg tacaccaccc tggctgggaa gagcgactcg
    11161 tctttggagg gcttgcggag gatgtcgccc acgccgccac cgttcctcag tccgtggttc
    11221 atgacggtga caatacacat aagcagagta tcacaggccc gctctgtgct gtccagctcc
    11281 tcgtcctctt ctaggatctc aggcaccgag acctcagaga cacagtccat cttgtcccca
    11341 ctacatgtgc ccataaatgt ggcagctccg tgtggcatcc ccagggtgct ggctctggag
    11401 tggttcccag gcagccggtc cacctccagg atgaagtcat ccttgaggaa gaggaagccc
    11461 acgatggaga agaggtagac aaggatgagg gccagcaggg cagttagcag gatggagcgg
    11521 ccattgcggg tcacgctctt gatgacatta aacagcgtct cctctcggta gatgaggtca
    11581 aagagcagga tactgtagaa gagctcatga gcaaagaggc ccaggacact cgtcaggatg
    11641 tagcccacgt gatacaggaa ctccatgtcc atgaccattg ctttatagcc acggatgaag
    11701 gtgccgcggt tgcccacaaa gctcaccaca aacacgatct tgttggtcag attgagagca
    11761 cccaggatgt tgagcgtggg cccaatgccc aggtagtaga tggagcgaag gaccagtgcc
    11821 acaatgaggg gacggacgct gtagtgcttg gtgaacagcg cggcaatgga gaagcagatg
    11881 aggatccaga agaggagcga gatgagtggg gagcccagca cgcccgtgga cgcgccttcc
    11941 acgtaggggt agaagaaggc aatgatgatg ttgatgaaca ctgccaggtt gaaggagatg
    12001 ctaccccaca gcgtcatgcg gcgggagaac cagtagatga gcggcatgct gcgaagccgg
    12061 cgctgccact ccatctcgtt gtgcaggaag gaggactggt cgaagaagtc gctcactttg
    12121 ctgccctgct cgtcctgctc cgtggtggtg aagagccggt gcttggtctc ctctgtcagg
    12181 aactggcaga tggcaggtac cgggaacacg atctgctcca tgcttcggtc ttgccgcaca
    12241 atctcaatct gcgaggtgtg gttctcatag taggccagcg ggtcttcctc ctcctcttgg
    12301 gcaggggctg aggacttgag catctgagag agctgcttgt tgttgaggct gagcatggaa
    12361 gagatgccct cggcctcctc ctcctggatg cgcttcaccg gcttcagcag gtgctgtagc
    12421 tgcttgttgt gcctggaaag ctgcagtgcc aggatgtaga tgttgtggcc cacttcacgt
    12481 gggctcactt cggaattttc ccgctcctcc tcctgcaggt aggccttctt gatcacgtcc
    12541 accagctcct gaggccgaag gctgatgagg atgcgttctg cattctcgct gtcatgtcga
    12601 ctctccatca gagccaggag cagcttggag gcattgtcct tgagctgcag caccaggtcc
    12661 atgcggtact tgcagagcgg gctgatgtca ttgaggatca gcgccgtgat gatgtcaatg
    12721 ccgttggact cgtgagtcac gatgcaggtc tggttctcat ggcacgggcc ctggcagtac
    12781 tctgtgaggg tctccaaggt ctggatgacc aggcccacat tgtcctcgtt gatgtagagc
    12841 cccagcagcc ccaggccccc tgtggtgctg ccacacatga tgtccaggaa ctgcagggtc
    12901 tcacacacga ggttgtagtt ggtcttgttg ttctgacagc gcaggaagtt ctgcaggtcc
    12961 cggttatggt tctcacacag cagctgcagg aagcgcagga tgggccgcat gatgagcacc
    13021 gaggtcccca tctcgttgtt ctgcgcacgc tcgctcacgt cgtgcccccg gtgcaagcca
    13081 gggcccagcg aatatcggga ggagctgggc atggagaagg aggacacgcg acctttggtg
    13141 gtagggtctg ctggctcacg gtcctcacga ggctggctgc ccaggtcact catgttgacg
    13201 gcaactgtgg acttggtctc ctgctgggca cgcttcatgc ggtcatgcag cactttgaag
    13261 aagcgctctg acttcttgtc acttgtcatc aggttgtaga aagacttctg gatctcagtg
    13321 ttgcccccgt ccagcaggcg gatggccagg ccgatgctct cctggaagat cttctcgttc
    13381 ttggtgct
//
