LOCUS       sequence-473613-       13388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transient overexpression of IP3R3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13388)
  AUTHORS   Kuchay S, Giorgi C, Simoneschi D, Pagan J, Missiroli S, Saraf A, 
            Florens L, Washburn MP, Collazo-Lorduy A, Castillo-Martin M, 
            Cordon-Cardo C, Sebti SM, Pinton P, Pagano M
  TITLE     PTEN counteracts FBXL2 to promote IP3R3- and Ca(2+)-mediated 
            apoptosis limiting tumour growth.
  JOURNAL   Nature. 2017 Jun 22;546(7659):554-558. doi: 10.1038/nature22965. 
            Epub 2017 Jun 14.
  PUBMED    28614300
REFERENCE   2  (bases 1 to 13388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature22965"; journalName: "Nature"; date: "2017-06-22- 
            22"; volume: "546"; issue: "7659"; pages: "554-558"
COMMENT     Sequence Label: pEGFPc1 IP3R3 WT
FEATURES             Location/Qualifiers
     source          1..13388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(4507..5217,5218..5220,5221..5223))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(4507..5217,5218..5220,5221..5223))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4549..4570)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4898..4917
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5157..5178
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5220..5229
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5268..5471)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5297..5317)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5472..5775
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(5950..6538)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6030..6049)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    6867..6914
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     6939..6958
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(7146..7940)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(7146..7940)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7257..7276)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7867..7886
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7975..8332)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7989..8124
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        8141..8332
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     8289..8309
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(8334..8438)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8465..8920
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8552..8571)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8762..8783
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    8927..9048
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8938..8957)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8992..9011
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     regulatory      13291..13300
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctgaaccaca attgtctgat gcgtctgtag ggatgtgctg ttctctgaaa atggggaact
       61 gaagaaggcg ctgatggtgt ccagcaccac ggtgagcacg tacttctcaa gggtggggtc
      121 tgacaggcgc ttctcccgct tgttacagac cagagccatg tccagggtga agttctcgaa
      181 gagcgtccag atgtggttgc tggtatagat ctccttcatc tccacctccg tgtctacata
      241 gcagtggttc acgaagttca catacgccat tttaacctcc gtgatgcagt cctcgtgggt
      301 caccaccgac accacgtcct ctagaggcag cagggaagtg cacttgatct ctgtgtagac
      361 atttttgcct tctgcacagg cagccagcaa gtccaccagg gagatgtggt acatgagggg
      421 gctgtggtcc tccacgccat ctcgggctgc cttcatcatg tccagcagat gggccagaga
      481 gtccttgtca ttgtagaaca ccaccacgtc gtcgcctgcg ttggtcagct cggtcatgat
      541 catgtcctga cacttcttca cgtacttgcc ctcggccttg atgacggtat gcaggaagtc
      601 caggtactgc acgtggcgcc cgtgtgtggc cagcaggtgc acgaagtgtt gcagcaccgg
      661 ctcgctgatc tcagagcaca gctgatagtt gttgaggaaa atgtgctgca tggtctcagc
      721 ctccaggagc ccgggcgtga ggaagagctg caagtgcttg tgcagaaggg cctggttgcc
      781 agggttcccg gcacagaact tctgtaggaa ctggtgtgtg tagcgcagga tctccatcat
      841 cttgttgtcg ttcttgtcat aagggatctg cagcaggtcc agcatgacct tgtgggcgtc
      901 catgttcttc agcagtctct gttgcttctt tcgcatctgc tccccgaccc cacacatctt
      961 gttcagcctc tccaggatgc ctttgacaat ctggtagttc tcactgctct tctccccgtg
     1021 tggctgcaga aatccctcct catccgaggg ccgctccttc ttatccttgc tggcgcccgc
     1081 ctccccctcc tcgcccttga cgctgccttt cttgtccacc cacagctccg acttctccac
     1141 catcgtccgc agtcgatcca gttccgactt gatcactttg tagttctcca catcctgggc
     1201 tgagatgagc agctgcacct gcttgaaggt gtgcatggcc tcctggcgct ggctgaaatg
     1261 tttgaagagc agctgcaggg cgccggagac cagaggtggg tagtcgtgca tggtgaggtg
     1321 cagcagcact cgcaggaaca tgcgaccccc ctcgtcatcc acctctagca tgctgctggt
     1381 cttccctact ccaaacatgg cctctgcctg ctccccgatg cggtccagat tcatgttggc
     1441 agtagaggaa tcgaaggcgg gggctgtgcc gtctgccccg ctgtcctgca tgggaaagac
     1501 ctccacaaac tccttcttga agaccgacag caggtaggag atgcggtagt ctaggcgcac
     1561 gttgaggatg aactgcagga tctccaggat cttcagcttg gtctccatca ccacggtgtg
     1621 ttcattgtcc tcaaatttgc ttctgtccag ctgctcgggg acacccactc cagtaggcag
     1681 gctggaggca ccaaatacag actgcttgcg gcttagcacc atggtggaca tcatgtgtcc
     1741 caccccctgg atggacctcc gcacgttctt gccgccaggc tcctcatagg cctgcagcac
     1801 agcggctggt gcctgaatgc agtcgatgat gcccagcaac gtgcgtgtga gtcgcagcag
     1861 ctcgctgaag ctgtagaacc cgaagtagat gaggttgtgt gccaggctga ccacctcaaa
     1921 ggtcagtatg ttcttctcaa cgttggcaaa gggcacggcc tcgccgacca cgttgttaag
     1981 gtaatcctcc acgaactcca tggtgctggc aaacttgttt ttcttgtcat ctcgggaggc
     2041 gttgagattg gaatcatagt ctttgatggt gatggccgtg gggatctctg tccagaggcg
     2101 ggcaaacttg acaggggtca ccagctcctg ggggtcacgg tccacgtgca catgtagcat
     2161 caggtggcag aatgaggcgc ggaggtcaaa gggcagcatc tcgtccgcca tgcataggaa
     2221 aagcagctcc acacccagtt gcttggagat ctcgtcgatg gccaggtact ggcggtctag
     2281 gcacatgcgg gcaaacagct tcaactggta cctgtagtag ctgagcacgt tctcgtcgtg
     2341 tgcgttgccg gcccttgcct cctgggccag ctgcctcaca ctcttctcgt gatgctcgtt
     2401 gttcctgtcc gtccatgtga gccacacctc ctcctccgag tactcgatgc tgaggtactc
     2461 gtgagactga gccatctcct tcacgggccg aagctcagtc tggataagga tgtcactgtt
     2521 cttggggtcc agcacacact tgcaaatcag ctcctgggtg acggggatgg caatgcggtt
     2581 ggacacgcac aggtcggaga ggtagtccag gaacctgggc tccctgttct tgcgcacgag
     2641 gctcacgaag gtttccacct cggtcttggt gatgtgcttc tccagcagct tccggttgtt
     2701 gtgcagcagg gccgtgatgg tgtcttcggc caggatgtca tagccaatct gtgactgcat
     2761 catcccgaac tgcttggcta tgtgctcctg gttcttacgg tagtcctcct gggagtgccg
     2821 cagcacccgg taacacaggc ggaacatgta ctggtagggg gcgttcttct ggtcagacag
     2881 ttcctccaga cgcaccaatg gcccttcacc ccccttgtca cggaatggag ccttcaggat
     2941 gccaaagatc tgcttaagta tgttctgctc ccgcatcagc ttctgccgct ctcggttggg
     3001 tttggtgacc atgatgtcca ggacattctg cccattgttg ggaacatcgc tgacaaaaaa
     3061 caccaagtcc tccaacagct ggatgacaaa cctgcggtcg ttctggctga tgaagccctc
     3121 gttgagtttc tccacagcgc tggccagcat ggagcttgca tcgttggcaa agtccaggtc
     3181 gcggatctca gacacgggaa ccgacacaat ggcaaaggcc tccttgtcct ccttggtggg
     3241 gcacgtgccc aacatgagcc ggataggccg ttcctcctcc acgtcgatgg gcgcgttcgt
     3301 gctctggatc caggtgttgg tacagaggtg ccgcagccgc acgtaggagt tccggggcac
     3361 gaaggagtcg gttttctgca gcgtggtggg gtccagctcg aagagagacg cgatgtcgtt
     3421 gccatgtggc acagccacca gacggtactt gatcttctcg ccggcgtttc tgcggcctgt
     3481 gcggctctgg gcccctggtc ctgctgcctt aggatctgag acatcaccct tgtagctggg
     3541 gttttcctca gccgccagat agttgcccgt ggccaggtgc ttgaacctgt acagaccatt
     3601 ccagtgtccc gctccgccgc ggcaggggtc atggtggacc acctccacct cccagagcgc
     3661 gttggagctg gtggccgagg tggcggactg acgcagcgtg gtcctcagga acacctgaag
     3721 cttgccccgg tactcgtcac aggtcaggaa cttctcctgc tctgcatgga acagccgcac
     3781 cacgtctccc cccttcaaca cctcctccag atggtcccgg aactgcatga ataagttgat
     3841 cttccagctg gtgttgcagt tgacagagtt gacctccttg cagccaacgt tatcactaag
     3901 ctcataattg ctggcgtgta gaggctgccc tgcgttgaca gggttcagga tcaccttgtc
     3961 ccccacaacc acgttatccc cattgctccg aagcttccag aagggctgga tgaagagcca
     4021 ggagccctcg ttgcccgtcg cgtccagcgt cacccgcatg gcgttcttct ccagcagggc
     4081 cggctgccgc ttgttcaccg ttaagtattt gttgctcttc atgtgcagaa gctgaatcac
     4141 gctgccgtac ttcaccacgt ccccgtgcac cttcttgttc tctgtgtcgt tttgcttctg
     4201 ctccatctgg gctgcatgct gcagcttctg cagcaacacc acatcggcga tcttctcttt
     4261 gtcctgctta gtctgcttgg ctttccagta ctgcttctgt gcggagtagc ggttcatggg
     4321 gcacactttg aagaggcaat ctcggaactt cttggggggg ttgtccaggt ccccagctgc
     4381 aggctccacc acacaacggt catccaccag ccccaaagtg ctgatgaagc cgttgacaga
     4441 gccctcggcg tacagggaca caatgtcccc gatgtgaaga aagctggaca tttcattcat
     4501 tccggacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
     4561 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag
     4621 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
     4681 gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat
     4741 gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag
     4801 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt
     4861 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt
     4921 gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg
     4981 ctgcttcatg tggtcggggt agcggctgaa gcactgcacg ccgtaggtca gggtggtcac
     5041 gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt
     5101 gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc
     5161 gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac
     5221 catggtggcg accggtagcg ctagcggatc tgacggttca ctaaaccagc tctgcttata
     5281 tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga
     5341 cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg
     5401 tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa
     5461 aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag
     5521 tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca
     5581 atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt
     5641 aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac
     5701 gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc
     5761 gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat
     5821 tactattaat aactaatgca tggcggtaat acggttatcc acagaatcag gggataacgc
     5881 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5941 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6001 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6061 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6121 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6181 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6241 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6301 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6361 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6421 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6481 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6541 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6601 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6661 aagttttaaa tcaatctaaa gtatatatga gtaacctgag gctatggcag ggcctgccgc
     6721 cccgacgttg gctgcgagcc ctgggccttc acccgaactt ggggggtggg gtggggaaaa
     6781 ggaagaaacg cgggcgtatt ggccccaatg gggtctcggt ggggtatcga cagagtgcca
     6841 gccctgggac cgaaccccgc gtttatgaac aaacgaccca acaccgtgcg ttttattctg
     6901 tctttttatt gccgtcatag cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt
     6961 tagcctcccc ctagggtggg cgaagaactc cagcatgaga tccccgcgct ggaggatcat
     7021 ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg cggcggtgga
     7081 atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga accccagagt
     7141 cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg
     7201 gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata
     7261 tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg
     7321 atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg
     7381 gtcacgacga gatcctcgcc gtcgggcatg ctcgccttga gcctggcgaa cagttcggct
     7441 ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc
     7501 cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga
     7561 tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca
     7621 aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc
     7681 gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat
     7741 agccgcgctg cctcgtcttg cagttcattc agggcaccgg acaggtcggt cttgacaaaa
     7801 agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc
     7861 tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc
     7921 aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc gatctttgca
     7981 aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc
     8041 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga
     8101 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta
     8161 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct
     8221 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
     8281 ctttccacac cctaactgac acacattcca cagctggttc tttccgcctc aggactcttc
     8341 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     8401 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     8461 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     8521 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     8581 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     8641 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     8701 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     8761 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     8821 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     8881 tttaacgcga attttaacaa aatattaacg cttacaattt acgcgttaag atacattgat
     8941 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     9001 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     9061 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     9121 aacctctaca aatgtggtat ggctgattat gatcagttat ctagatccgg tggatcccgg
     9181 gcccgcggta ccgtcgactg cagattcgcg gccgcttttt ttggagtcta ttaatttatt
     9241 taaggcaatt aacacattag tctccaggac acaggacatg taaaacatta caccaaaaag
     9301 agagaagcca ctgtcacaca gcccaggcct gttgctgggc ttgtgaggct tgcctacaaa
     9361 gcctagagaa agtcctggga gcaaggtcca ccctgagtca tgaggctcct gggacggggc
     9421 cacactgtcc ccagcaggca caggagggtg ggatggcccc caggaataag ttaaatatca
     9481 aagccctagg aagctaataa cagcaggcta ccaccttcct gagccagggt ggcaggcagg
     9541 ggacagccac aatgtagtgc ttggaggtga gaggcagccc acttttaacc acctgccagg
     9601 gccactgagg aatgccagtc actgcttcga aggacagcag tctggaggac ttggcatccc
     9661 ctaaggcact aaggtaaacc gagtctcttc aagggtactt actaagcagg accgtgctct
     9721 gctctggcac tcccgctggc cagagcctgg cttggggagg gagggtggca gctgagctga
     9781 agatggggca ggcacctggc ttctacctga cctgaaggaa gggcggcgct gtgggcagcc
     9841 ggcctcccgt gggggcctcc gaggctccgc tctgctcagc ggctcatgca gttctgcacg
     9901 tccacgaagc ccagacgttg cctccgcttc cgctgctccg tcatctgttc cttgagctca
     9961 ttgagctggg cggtgaggtg cgagaccagc ttcatggtgg agccaagctt ctcctgcagg
    10021 atgcggatct cgttctgctc gccctcgccc tcgccgctca ccaaggacat ggcgcgcatc
    10081 cgtgggaacc agtccaggtt cttgttcttg atcatctgag ccacgtagct ctcggggccc
    10141 gtgtagtctg tcttgttctt gacacggacc agcacaatga agtacaggta gttccacatg
    10201 ttgtgctcca gtttgatgtg ctcttcaaag gacactgtct tgttgtcaaa cttgtccctc
    10261 tccagaccac agatgaagca cgtggtcttg aggatttcct ctttcttctg cttctcgctg
    10321 cgcaggtcgg cgaaggtgtc gatgatcacc ccaaagatga ggttcaggac aataatgatg
    10381 acaatgaaga agaacaggag gtcgtacacc accctggctg ggaagagcga ctcgtctttg
    10441 gagggcttgc ggaggatgtc gcccacgccg ccaccgttcc tcagtccgtg gttcatgacg
    10501 gtgacaatac acataagcag agtatcacag gcccgctctg tgctgtccag ctcctcgtcc
    10561 tcttctagga tctcaggcac cgagacctca gagacacagt ccatcttgtc cccactacat
    10621 gtgcccataa atgtggcagc tccgtgtggc atccccaggg tgctggctct ggagtggttc
    10681 ccaggcagcc ggtccacctc caggatgaag tcatccttga ggaagaggaa gcccacgatg
    10741 gagaagaggt agacaaggat gagggccagc agggcagtta gcaggatgga gcggccattg
    10801 cgggtcacgc tcttgatgac attaaacagc gtctcctctc ggtagatgag gtcaaagagc
    10861 aggatactgt agaagagctc atgagcaaag aggcccagga cactcgtcag gatgtagccc
    10921 acgtgataca ggaactccat gtccatgacc attgctttat agccacggat gaaggtgccg
    10981 cggttgccca caaagctcac cacaaacacg atcttgttgg tcagattgag agcacccagg
    11041 atgttgagcg tgggcccaat gcccaggtag tagatggagc gaaggaccag tgccacaatg
    11101 aggggacgga cgctgtagtg cttggtgaac agcgcggcaa tggagaagca gatgaggatc
    11161 cagaagagga gcgagatgag tggggagccc agcacgcccg tggacgcgcc ttccacgtag
    11221 gggtagaaga aggcaatgat gatgttgatg aacactgcca ggttgaagga gatgctaccc
    11281 cacagcgtca tgcggcggga gaaccagtag atgagcggca tgctgcgaag ccggcgctgc
    11341 cactccatct cgttgtgcag gaaggaggac tggtcgaaga agtcgctcac tttgctgccc
    11401 tgctcgtcct gctccgtggt ggtgaagagc cggtgcttgg tctcctctgt caggaactgg
    11461 cagatggcag gtaccgggaa cacgatctgc tccatgcttc ggtcttgccg cacaatctca
    11521 atctgcgagg tgtggttctc atagtaggcc agcgggtctt cctcctcctc ttgggcaggg
    11581 gctgaggact tgagcatctg agagagctgc ttgttgttga ggctgagcat ggaagagatg
    11641 ccctcggcct cctcctcctg gatgcgcttc accggcttca gcaggtgctg tagctgcttg
    11701 ttgtgcctgg aaagctgcag tgccaggatg tagatgttgt ggcccacttc acgtgggctc
    11761 acttcggaat tttcccgctc ctcctcctgc aggtaggcct tcttgatcac gtccaccagc
    11821 tcctgaggcc gaaggctgat gaggatgcgt tctgcattct cgctgtcatg tcgactctcc
    11881 atcagagcca ggagcagctt ggaggcattg tccttgagct gcagcaccag gtccatgcgg
    11941 tacttgcaga gcgggctgat gtcattgagg atcagcgccg tgatgatgtc aatgccgttg
    12001 gactcgtgag tcacgatgca ggtctggttc tcatggcacg ggccctggca gtactctgtg
    12061 agggtctcca aggtctggat gaccaggccc acattgtcct cgttgatgta gagccccagc
    12121 agccccaggc cccctgtggt gctgccacac atgatgtcca ggaactgcag ggtctcacac
    12181 acgaggttgt agttggtctt gttgttctga cagcgcagga agttctgcag gtcccggtta
    12241 tggttctcac acagcagctg caggaagcgc aggatgggcc gcatgatgag caccgaggtc
    12301 cccatctcgt tgttctgcgc acgctcgctc acgtcgtgcc cccggtgcaa gccagggccc
    12361 agcgaatatc gggaggagct gggcatggag aaggaggaca cgcgaccttt ggtggtaggg
    12421 tctgctggct cacggtcctc acgaggctgg ctgcccaggt cactcatgtt gacggcaact
    12481 gtggacttgg tctcctgctg ggcacgcttc atgcggtcat gcagcacttt gaagaagcgc
    12541 tctgacttct tgtcacttgt catcaggttg tagaaagact tctggatctc agtgttgccc
    12601 ccgtccagca ggcggatggc caggccgatg ctctcctgga agatcttctc gttcttggtg
    12661 ctggtgatga gatcacacac caacttggtg gccccctcct tgtccagccg gcactgggtg
    12721 gctgcgatgg cggaccagtc ctgatccacg ccagagcctg tggggtcagt gagctcgccc
    12781 cggggaccgg acttccggtt ctggaggtaa ttctgcagca gcatcttcct tagctggttg
    12841 ccccggtccc catacttgct cttcttctgc agcatctgct gcagcgtccg cagcaccttc
    12901 acgcacagct tctcctccga ctccatgaga cccttggtgt gacggatgag cttggacagg
    12961 aagccgccac tctcgcagcg ctggtaggcc tcactgccct ctagaaagag cagctcgggc
    13021 cagtgcagca tgtccaccag caccgagagc tcggcctgca ccagaggctt cagccgctct
    13081 tctagggccg tgatgatgtc ctgtaacttc tcaatgatgt tcttgtagtc ccactggttg
    13141 gcggtgggga tgacccgagg gaaggtcctc gtggccgtct tgtagttggc agcgctgcgt
    13201 tgggccgcgg ctgagcagct gcccccactg ctgagcagag cgctcatgtg ggcatccaga
    13261 tccatcggca gcaagatggc tctactcttg gccaccatgg cgagggtgcg gacacaggcc
    13321 tccaccgagc ccttgtgctg ctgctgcagc caaggacact cgagcagccg ggtggtggac
    13381 tgcagcag
//
