LOCUS       sequence-473615-        9114 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A sleeping beauty-based vector containing CAG promoter-driven 
            mCherry-P2A-EIF3D S528D/S529D-IRES-Pac..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9114)
  AUTHORS   Okubo C, Nakamura M, Sato M, Shichino Y, Mito M, Takashima Y, 
            Iwasaki S, Takahashi K
  TITLE     EIF3D safeguards the homeostasis of key signaling pathways in human 
            primed pluripotency.
  JOURNAL   Sci Adv. 2025 Apr 11;11(15):eadq5484. doi: 10.1126/sciadv.adq5484. 
            Epub 2025 Apr 9.
  PUBMED    40203091
REFERENCE   2  (bases 1 to 9114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-04-11"; volume: "11(15):eadq5484. doi"; pages: " 
            10.1126/sciadv.adq5484. Epub 2025 Apr 9"
COMMENT     Sequence Label: SB-CAG-mCherry-P2A-EIF3D-DD-IP
FEATURES             Location/Qualifiers
     source          1..9114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        2481..2860
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          3140..4156
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4080..4102
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4164..4183
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             4232..4939
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4377..4395)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4645..4664
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4955..5011
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    join(6682..7256,7257..7259,7260..7268)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6849..6866)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7076..7095
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7269..7868
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7269..7288)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7765..7785
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7949..7966)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7955..8179
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8470..8489)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8471..8489)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8499..8516)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8499..8515)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8508..8530)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8657..9112
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8744..8763)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8954..8975
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg
     2221 gccggatccc tatacagttg aagtcggaag tttacataca cttaagttgg agtcattaaa
     2281 actcgttttt caactactcc acaaatttct tgttaacaaa caatagtttt ggcaagtcag
     2341 ttaggacatc tactttgtgc atgacacaag tcatttttcc aacaattgtt tacagacaga
     2401 ttatttcact tataattcac tgtatcacaa ttccagtggg tcagaagttt acatacacta
     2461 agttgctagc ggcgcgcctc gacattgatt attgactagt tattaatagt aatcaattac
     2521 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     2581 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     2641 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     2701 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     2761 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     2821 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
     2881 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
     2941 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc crgggggggg
     3001 gggggggggs grggggsggg gsggggsgrg gcggrgrggk gcggcggcag ccaatcagag
     3061 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
     3121 gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc
     3181 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
     3241 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
     3301 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag
     3361 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
     3421 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
     3481 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
     3541 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg
     3601 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc
     3661 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt
     3721 gggggtgccg ggcggggcgg ggccgcctcg ggccggggrg ggctcggggg aggggcgcgg
     3781 cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg
     3841 gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc
     3901 tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag
     3961 gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct
     4021 ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg
     4081 gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt
     4141 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg
     4201 caaagaattc ccttaaggtt acgccaccat gatggtgagc aagggcgagg aggataacat
     4261 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     4321 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     4381 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     4441 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     4501 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     4561 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     4621 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     4681 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     4741 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     4801 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     4861 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     4921 catggacgag ctgtacaagt acaagggatc tggcgccacg aacttctctc tgttaaaaca
     4981 agcaggagac gtggaagaaa atcccggtcc gatggcaaag ttcatgacac ccgtgatcca
     5041 ggacaacccc tcaggctggg gtccctgtgc ggttcccgag cagtttcggg atatgcccta
     5101 ccagccgttc agcaaaggag atcggctagg aaaggttgca gactggacag gagccacata
     5161 ccaagataag aggtacacaa ataagtactc ctctcagttt ggtggtggaa gtcaatatgc
     5221 ttatttccat gaggaggatg aaagtagctt ccagctggtg gatacagcgc gcacacagaa
     5281 gacggcctac cagcggaatc gaatgagatt tgcccagagg aacctccgca gagacaaaga
     5341 tcgtcggaac atgttgcagt tcaacctgca gatcctgcct aagagtgcca aacagaaaga
     5401 gagagaacgc attcgactgc agaaaaagtt ccagaaacaa tttggggtta ggcagaaatg
     5461 ggatcagaaa tcacagaaac cccgagactc ttcagttgaa gttcgtagtg attgggaagt
     5521 gaaagaggaa atggattttc ctcagttgat gaagatgcgc tacttggaag tatcagagcc
     5581 acaggacatt gagtgttgtg gggccctaga atactacgac aaagcctttg accgcatcac
     5641 cacgaggagt gagaagccac tgcggagcat caagcgcatc ttccacactg tcaccaccac
     5701 agacgaccct gtcatccgca agctggcaaa aactcagggg aatgtgtttg ccactgatgc
     5761 catcctggcc acgctgatga gctgtacccg ctcagtgtat tcctgggata ttgtcgtcca
     5821 gagagttggg tccaaactct tctttgacaa gagagacaac tctgactttg acctcctgac
     5881 agtgagtgag actgccaatg agccccctca agatgaaggt aattccttca attcaccccg
     5941 caacctggcc atggaggcaa cctacatcaa ccacaatttc tcccagcagt gcttgagaat
     6001 ggggaaggaa agatacaact tccccaaccc aaacccgttt gtggaggacg acatggataa
     6061 gaatgaaatc gcctctgttg cgtaccgtta ccgcaggtgg aagcttggag atgatattga
     6121 ccttattgtc cgttgtgagc acgatggcgt catgactgga gccaacgggg aagtgtcctt
     6181 catcaacatc aagacactca atgagtggga ttccaggcac tgtaatggcg ttgactggcg
     6241 tcagaagctg gactctcagc gaggggctgt cattgccacg gagctgaaga acaacagcta
     6301 caagttggcc cggtggacct gctgtgcttt gctggctgga tctgagtacc tcaagcttgg
     6361 ttatgtgtct cggtaccacg tgaaagactc ctcacgccac gtcatcctag gcacccagca
     6421 gttcaagcct aatgagtttg ccagccagat caacctgagc gtggagaatg cctggggcat
     6481 tttacgctgc gtcattgaca tctgcatgaa gctggaggag ggcaaatacc tcatcctcaa
     6541 ggaccccaac aagcaggtca tccgtgtcta cagcctccct gatggcacct tcgacgatga
     6601 tgaagatgag gaggaagagg aggaggaaga agaggaagaa gaagaggaag aaacttaaat
     6661 cggccgcaat tccgcccccc ccccctctcc ctcccccccc cctaacgtta ctggccgaag
     6721 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     6781 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     6841 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     6901 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     6961 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     7021 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     7081 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     7141 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     7201 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     7261 ccacaaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     7321 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     7381 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     7441 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     7501 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     7561 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     7621 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     7681 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     7741 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     7801 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     7861 gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg
     7921 catcgatgat ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     7981 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     8041 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     8101 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     8161 gatgcggtgg gctctatggc ttctgaggcg gaaagaacct gcagcgagtg tatgtaaact
     8221 tctgacccac tgggaatgtg atgaaagaaa taaaagctga aatgaatcat tctctctact
     8281 attattctga tatttcacat tcttaaaata aagtggtgat cctaactgac ctaagacagg
     8341 gaatttttac taggattaaa tgtcaggaat tgtgaaaaag tgagtttaaa tgtatttggc
     8401 taaggtgtat gtaaacttcc gacttcaact gtatagggat cctcgagggg gggcccggta
     8461 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     8521 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     8581 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     8641 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     8701 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     8761 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     8821 ttagggttcc gatttagtgc ttgacggcac ctcgacccca aaaaacttga ttagggtgat
     8881 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     8941 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     9001 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     9061 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttag
//
