LOCUS       sequence-473617-        7864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  stable expression of p100 using virus infection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7864)
  AUTHORS   Busino L, Millman SE, Scotto L, Kyratsous CA, Basrur V, O'Connor O, 
            Hoffmann A, Elenitoba-Johnson KS, Pagano M
  TITLE     Fbxw7alpha- and GSK3-mediated degradation of p100 is a pro-survival 
            mechanism in multiple myeloma.
  JOURNAL   Nat Cell Biol. 2012 Mar 4;14(4):375-85. doi: 10.1038/ncb2463.
  PUBMED    22388891
REFERENCE   2  (bases 1 to 7864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Cell Biol"; date: "2012-03-4- 4"; volume: "14"; issue: "4"; 
            pages: "375-85"
COMMENT     Sequence Label: pBABE FLAG HA p100 (Ser707/711Ala)
FEATURES             Location/Qualifiers
     source          1..7864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             129..728
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(129..148)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     625..645
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             949..1418
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(1548..1565)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1719..2307)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1799..1818)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2467..3258,3259..3327))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3090..3109
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3328..3432)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             3463..3932
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    3997..4196
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             4397..4813
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     4743..4765
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     4781..4797
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             4842..4865
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4878..4904
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             4878..4904
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(7649..7669)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(7654..7864,1..119)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(7834..7864,1..105)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
       61 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      121 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
      181 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
      241 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
      301 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
      361 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
      421 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
      481 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
      541 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
      601 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
      661 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
      721 gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg
      781 catcgataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca
      841 cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat
      901 aactgagaat agagaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc
      961 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa
     1021 cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
     1081 caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga
     1141 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc
     1201 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac
     1261 aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc
     1321 caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc
     1381 ctctgagtga ttgactaccc gtcagcgggg gtctttcaca tgcagcatgt atcaaaatta
     1441 atttggtttt ttttcttaag tatttacatt aaatggccat agttgcatta atgaatcggc
     1501 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     1561 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1621 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1681 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1741 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1801 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1861 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1921 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1981 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2041 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2101 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     2161 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2221 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2281 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2341 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2401 ttcacctaga tccttttgcg gccgcaaatc aatctaaagt atatatgagt aaacttggtc
     2461 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2521 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     2581 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     2641 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     2701 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     2761 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     2821 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     2881 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     2941 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     3001 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     3061 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     3121 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     3181 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     3241 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     3301 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     3361 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     3421 aggggttccg cgcacatttc cccgaaaagt gccacctgca gcctgaatat gggccaaaca
     3481 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg
     3541 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     3601 cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc
     3661 cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg
     3721 cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc
     3781 tcactcgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa
     3841 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga
     3901 gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac
     3961 ccctgcccag ggaccaccga cccaccaccg ggaggcaagc tggccagcaa cttatctgtg
     4021 tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt actagttagc
     4081 taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa cacccggccg
     4141 caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga cctgaggaag
     4201 ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag acgagaacct
     4261 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa gccgcgcgtc
     4321 ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg tgtttctgta
     4381 tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct taggtcactg
     4441 gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga gacgttgggt
     4501 taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag acggcacctt
     4561 taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc cgcatggaca
     4621 cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc cccctccctg
     4681 ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg ccccgtctct
     4741 cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag ccctcactcc
     4801 ttctctaggc gccggccgga tcctccgctc gatgaaccat ggactacaag gacgacgatg
     4861 acaagctcga tggaggatac ccctacgacg tgcccgacta cgccgaagga agatccatgg
     4921 agagttgcta caacccaggt ctggatggta ttattgaata tgatgatttc aaattgaact
     4981 cctccattgt ggaacccaag gagccagccc cagaaacagc tgatggcccc tacctggtga
     5041 tcgtggaaca gcctaagcag agaggcttcc gatttcgata tggctgtgaa ggcccctccc
     5101 atggaggact gcccggtgcc tccagtgaga agggccgaaa gacctatccc accgtcaaga
     5161 tctgtaacta cgagggacca gccaagatcg aggtggacct ggtaacacac agtgacccac
     5221 ctcgtgctca tgcccacagt ctggtgggca agcaatgctc ggagctgggg atctgcgccg
     5281 tttctgtggg gcccaaggac atgactgccc aatttaacaa cctgggtgtc ctgcatgtga
     5341 ctaagaagaa catgatgggg actatgatac aaaaacttca gaggcagcgg ctccgctcta
     5401 ggccccaggg ccttacggag gccgagcagc gggagctgga gcaagaggcc aaagaactga
     5461 agaaggtgat ggatctgagt atagtgcggc tgcgcttctc tgccttcctt agagccagtg
     5521 atggctcctt ctccctgccc ctgaagccag tcatctccca gcccatccat gacagcaaat
     5581 ctccgggggc atcaaacctg aagatttctc gaatggacaa gacagcaggc tctgtgcggg
     5641 gtggagatga agtttatctg ctttgtgaca aggtgcagaa agatgacatt gaggttcggt
     5701 tctatgagga tgatgagaat ggatggcagg cctttgggga cttctctccc acagatgtgc
     5761 ataaacagta tgccattgtg ttccggacac ccccctatca caagatgaag attgagcggc
     5821 ctgtaacagt gtttctgcaa ctgaaacgca agcgaggagg ggacgtgtct gattccaaac
     5881 agttcaccta ttaccctctg gtggaagaca aggaagaggt gcagcggaag cggaggaagg
     5941 ccttgcccac cttctcccag cccttcgggg gtggctccca catgggtgga ggctctgggg
     6001 gtgcagccgg gggctacgga ggagctggag gaggtggcag cctcggtttc ttcccctcct
     6061 ccctggccta cagcccctac cagtccggcg cgggccccat gggctgctac ccgggaggcg
     6121 ggggcggggc gcagatggcc gccacggtgc ccagcaggga ctccggggag gaagccgcgg
     6181 agccgagcgc cccctccagg accccccagt gcgagccgca ggccccggag atgctgcagc
     6241 gagctcgaga gtacaacgcg cgcctgttcg gcctggcgca gcgcagcgcc cgagccctac
     6301 tcgactacgg cgtcaccgcg gacgcgcgcg cgctgctggc gggacagcgc cacctgctga
     6361 cggcgcagga cgagaacgga gacacaccac tgcacctagc catcatccac gggcagacca
     6421 gtgtcattga gcagatagtc tatgtcatcc accacgccca ggacctcggc gttgtcaacc
     6481 tcaccaacca cctgcaccag acgcccctgc acctggcggt gatcacgggg cagacgagtg
     6541 tggtgagctt tctgctgcgg gtaggtgcag acccagctct gctggatcgg catggagact
     6601 cagccatgca tctggcgctg cgggcaggcg ctggtgctcc tgagctgctg cgtgcactgc
     6661 ttcagagtgg agctcctgct gtgccccagc tgttgcatat gcctgacttt gagggactgt
     6721 atccagtaca cctggcggtc cgagcccgaa gccctgagtg cctggatctg ctggtggaca
     6781 gtggggctga agtggaggcc acagagcggc aggggggacg aacagccttg catctagcca
     6841 cagagatgga ggagctgggg ttggtcaccc atctggtcac caagctccgg gccaacgtga
     6901 acgctcgcac ctttgcggga aacacacccc tgcacctggc agctggactg gggtacccga
     6961 ccctcaccca cctccttctg aaggctggtg ctgacatcca tgctgaaaac gaggagcccc
     7021 tgtgcccact gcctgcaccc cctaccgctg atagcgactc ggactctgaa gggcctgaga
     7081 aggacacccg aagcagcttc cggggccaca cgcctcttga cctcacttgc agcaccaagg
     7141 tgaagacctt gctgctaaat gctgctcaga acaccatgga gccacccctg accccgccca
     7201 gcccagcagg gccgggactg tcacttggtg atacagctct gcagaacctg gagcagctgc
     7261 tagacgggcc agaagcccag ggcagctggg cagagctggc agagcgtctg gggctgcgca
     7321 gcctggtaga cacgtaccga cagacaacct cacccagtgg cagcctcctg cgcagctacg
     7381 agctggctgg cggggacctg gcaggtctac tggaggccct gtctgacatg ggcctagagg
     7441 agggagtgag gctgctgagg ggtccagaaa cccgagacaa gctgcccagc acagcagagg
     7501 tgaaggaaga cagtgcgtac gggagccagt cagtggagca ggaggcagag aagctgggcc
     7561 caccccctga gccaccagga gggctctgcc acgggcaccc ccagcctcag gtgcactgag
     7621 aattcgccag cacagtggtc gaccctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     7681 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     7741 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7801 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     7861 gccc
//
