LOCUS       sequence-473624-       10700 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transient overexpression of EMSY in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10700)
  AUTHORS   Marzio A, Kurz E, Sahni JM, Di Feo G, Puccini J, Jiang S, Hirsch CA,
            Arbini AA, Wu WL, Pass HI, Bar-Sagi D, Papagiannakopoulos T, Pagano 
            M
  TITLE     EMSY inhibits homologous recombination repair and the interferon 
            response, promoting lung cancer immune evasion.
  JOURNAL   Cell. 2022 Jan 6;185(1):169-183.e19. doi: 
            10.1016/j.cell.2021.12.005. Epub 2021 Dec 27.
  PUBMED    34963055
REFERENCE   2  (bases 1 to 10700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.12.005"; journalName: "Cell"; date: "2022-01-6-
            6"; volume: "185"; issue: "1"; pages: "169-183.e19"
COMMENT     Sequence Label: pcDNA3.1 2xFLAG-2xSTREP-EMSY (human)
FEATURES             Location/Qualifiers
     source          1..10700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(367..387)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        372..701
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      552..687
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             768..1562
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(822..841)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1432..1451
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1736..1857
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1773..1792)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1827..1846
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1906..1922)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1919..1941)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1930..1946
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1954..1960,1961..1978,1979..1984))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1999..2020
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2137..2154)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2308..2896)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2388..2407)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3067..3858,3859..3927))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3690..3709
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3928..4032)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4059..4514
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4146..4165)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4356..4377
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4640..4662
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4654..4671
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4655..4671
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        4832..5211
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5212..5415
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5366..5386
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5441..5460
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5441..5459
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5797..5808
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      8591..8600
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9493..9516
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             9532..9555
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             9571..9594
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             9631..9654
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             9676..10371
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     primer_bind     complement(10394..10410)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    join(10421..10700,1..343)
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(10620..10639)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt
       61 tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat
      121 ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg
      181 ctcccttccc tgtccttctg attttaaaat aactatacca gcaggaggac gtccagacac
      241 agcataggct acctggccat gcccaaccgg tgggacattt gagttgcttg cttggcactg
      301 tcctctcatg cgttgggtcc actcagtaga tgcctgttga attgggtacg cggccagctt
      361 ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
      421 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
      481 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
      541 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
      601 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
      661 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
      721 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
      781 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
      841 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
      901 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
      961 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     1021 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     1081 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     1141 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     1201 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     1261 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     1321 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     1381 gattcatcga ctgtggccgg ctgggtgtgg ccgaccgcta tcaggacata gcgttggcta
     1441 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     1501 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     1561 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     1621 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     1681 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     1741 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     1801 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     1861 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     1921 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     1981 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     2041 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     2101 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     2161 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     2221 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     2281 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     2341 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     2401 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     2461 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     2521 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     2581 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacatga
     2641 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     2701 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     2761 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2821 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2881 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2941 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     3001 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     3061 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     3121 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     3181 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     3241 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     3301 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     3361 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     3421 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     3481 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     3541 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     3601 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     3661 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     3721 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     3781 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     3841 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     3901 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     3961 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     4021 aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt
     4081 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4141 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4201 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4261 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4321 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4381 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     4441 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     4501 aacgcttaca atttccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     4561 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     4621 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
     4681 atctatacat tgaatcaata ttggcaatta gccatattag tcattggtta tatagcataa
     4741 atcaatattg gctattggcc attgcatacg ttgtatctat atcataatat gtacatttat
     4801 attggctcat gtccaatatg accgccatgt tgacattgat tattgactag ttattaatag
     4861 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     4921 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     4981 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     5041 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct
     5101 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg
     5161 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     5221 ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     5281 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     5341 tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     5401 tatataagca gagctcgttt agtgaaccgt cagaattttg taatacgact cactataggg
     5461 cggccgggaa ttcgtcgact ggatccggta ccgaggagat ctgccgccgc gatcgccatg
     5521 cctgttgtgt ggccaaccct tctggatctc agcagggatg aatgcaaaag aattcttcga
     5581 aaattggaat tggaggcata tgctggagtt atcagtgcac ttcgggcaca gggggatctc
     5641 accaaggaaa agaaagatct tcttggagaa ctatcaaaag ttcttagcat ctcaacagaa
     5701 cgccaccgtg ctgaagttcg gagagcagta aacgatgaac ggttaacaac aattgcacat
     5761 aatatgtctg gacctaatag ctcttcagaa tggtccattg aaggtcgtcg attggtacca
     5821 ctgatgcccc ggctcgttcc ccaaaccgcc tttactgtaa cagctaatgc tgttgctaat
     5881 gcagctatcc agcataatgc atctcttcca gtgcctgcag aaacaggaag caaggaagta
     5941 gtggtttgct attcctacac aagtaccacg tcaaccccaa cctctacccc tgttccaagt
     6001 ggcagcatag caacggttaa gtctccaaga cctgccagtc ctgcctccaa tgtagttgtc
     6061 ttgccaagtg gaagtactgt ttatgtcaaa agtgtaagct gttcagatga agatgaaaaa
     6121 cccagaaaac gaaggcgaac aaactcttcc agctcctctc ctgttgttct aaaggaagtt
     6181 ccaaaggccg ttgttccagt ctcaaagacg atcactgtgc ctgtgagtgg tagtcccaag
     6241 atgagcaaca tcatgcagag cattgccaac tccttaccac cccacatgtc tcctgtaaaa
     6301 ataaccttca ctaaaccatc aacacagaca acaaacacaa caacacagaa ggttattata
     6361 gtcaccacat caccaagctc aaccttcgtg cccaacattc tctccaaatc ccataactat
     6421 gcagcagtca ctaagcttgt accaacgtca gtcattgctt ctacaaccca gaagccacca
     6481 gttgttataa ctgcttcaca gtcctctctg gtcagtaata gcagcagtgg cagcagcagt
     6541 tctacaccat cacctattcc taatacagtt gcagtaacag ctgtggtgtc ctctacacca
     6601 tctgtggtca tgtcaacagt agcacaaggt gtatctacat cagcaatcaa aatggcatca
     6661 accagacttc cttcccccaa aagcttagtg agtgccccaa ctcagattct tgcacagttc
     6721 cctaaacaac atcaacagtc tcctaagcag cagttatatc aagtgcaaca gcagacacag
     6781 caacaagtgg cccagccttc tccagtatct catcagcaac agcctcagca gtctcctttg
     6841 ccacctggta ttaaacctac catccaaatc aaacaggagt caggtgttaa aatcatcaca
     6901 caacaggttc aaccaagtaa aatcttaccc aaaccagtga cagcaactct acccaccagt
     6961 agcaattccc ctattatggt ggttagcagt aatggtgcaa ttatgacaac taaactggta
     7021 accactccta ctggcacaca agcaacctat acccggccaa cagtgagccc atccattggt
     7081 cggatggctg caacccctgg agctgcaacc tatgtgaaaa ctacgagtgg tagcatcatt
     7141 acagtagtac ccaaatcatt agctaccttg gggggcaaga taattagcag taatatagtt
     7201 tctggaacga ctaccaaaat cactacaatc ccaatgactt ccaagcccaa cgtgattgtt
     7261 gtacaaaaga ctacaggaaa aggaacgacc attcaaggcc tcccgggcaa aaatgttgtc
     7321 acaacgttgc taaatgctgg aggagaaaag actattcaga cagtgccaac aggagcaaag
     7381 ccagctatcc ttactgctac aagacccatc accaaaatga ttgtaacgca gccaaaagga
     7441 ataggttcta cagttcaacc agcagctaaa atcatcccaa caaaaattgt ttatgggcag
     7501 caagggaaaa cgcaggttct tattaaaccc aaaccagtga cttttcaagc gacagttgtt
     7561 agtgaacaaa caagacagct agtaacagaa acattacagc aagcatccag ggtagcagag
     7621 gctggtaatt catctattca ggaaggaaaa gaagaaccac agaattatac agatagtagt
     7681 tcctcttcta cagagtcctc ccagagttcc caagattccc agcctgtagt tcatgtaatt
     7741 gcttcccggc gtcaggattg gtcagaacat gagattgcaa tggagactag ccctaccata
     7801 atttatcagg atgtatccag tgaatcacaa tcagctactt caacaatcaa agctctgtta
     7861 gaactccaac agacaacagt aaaggaaaaa ttggaatcta aaccaagaca acccactatt
     7921 gacctgagtc aaatggcagt gcctattcag atgacccagg aaaagagaca ttctcctgag
     7981 agtccatcaa ttgctgtggt agagtcagaa ctagtagctg aatacatcac tactgaacgc
     8041 actgatgagg ggacagaggt tgcttttccc cttctagtca gccatcgctc ccagccccaa
     8101 cagccttccc agccccagcg gaccctgctc cagcatgtgg ctcagtcaca gaccgcaaca
     8161 cagacttcgg tggtggtgaa gtccatccca gcatcttccc ctggagcaat cacccacatt
     8221 atgcagcagg cattaagcag tcacactgct tttaccaaac acagcgagga acttggaact
     8281 gaggagggcg aggttgaaga gatggacact ttagaccctc agacaggtct gttttaccga
     8341 tctgccctga ctcagtcaca gtcagctaaa cagcagaaac ttagccagcc cccgctggaa
     8401 cagactcagc tgcaagtgaa aactctgcag tgcttccaga ctaaacagaa gcagaccatc
     8461 cacctgcagg cagaccagct ccagcacaaa ctcccgcaaa tgccccagct ttccatcagg
     8521 catcaaaaac tcacccctct ccagcaagaa caagcacagc ccaagccaga tgtacagcac
     8581 acacagcatc ccatggtggc caaagacagg cagcttccta ccttaatggc acagcccccg
     8641 caaactgtag tacaggtgct tgcagtgaaa accacgcagc agctccctaa actgcagcag
     8701 gctccgaacc aaccaaaaat ctacgtgcaa ccccaaaccc cccagagcca aatgtcgctc
     8761 ccagcttctt cagagaaaca gacggcaagc caggtggagc agccaattat aacccaagga
     8821 tcctctgtta caaagataac ttttgagggg cgccagcctc ccacagttac aaagataact
     8881 ggtggcagtt ctgtgcctaa gctgacatca ccagttacaa gcatatctcc cattcaggcc
     8941 tctgagaaga cagcagtgtc tgacattttg aaaatgtctt tgatggaagc tcagattgat
     9001 acaaatgtag aacatatgat agtggatccc ccaaagaagg ctcttgccac tagcatgctc
     9061 actggtgaag caggatcatt accctccacc cacatggtgg tggcagggat ggcgaattcc
     9121 actccccagc aacagaaatg tagagagtcc tgttcgagtc catccactgt tggctcttcc
     9181 ctaacgacaa ggaaaattga tccaccagca gtgcctgcga caggccagtt catgcgtatt
     9241 cagaatgtag gccaaaagaa agctgaagag agtccagcag aaattatcat ccaggctatt
     9301 cctcagtatg ctattccttg tcactccagc tccaatgtgg tggtggagcc cagtgggctt
     9361 cttgagctaa acaacttcac tagtcaacag ctggatgatg aggagacagc aatggagcag
     9421 gacatagaca gtagcacgga ggatggaact gaacccagcc cttctcagag ctctgctgaa
     9481 cggtccacgc gtgactacaa ggacgacgat gacaaggtac caggatctat ggattataaa
     9541 gatgatgatg ataaagggtc ggccgccagc tggagccacc ctcagttcga gaagggagga
     9601 ggaagcggcg gaggcagcgg aggaggaagc tggagccacc cgcagttcga gaaaggataa
     9661 acgcggccgc tcgagatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc
     9721 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc
     9781 cccgagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc
     9841 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc
     9901 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc
     9961 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc
    10021 cgcgtgatcg gcgacttcaa ggtgatgggc accggcttcc ccgaggacag cgtgatcttc
    10081 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac
    10141 gatctggatg gcagcttcac ccgcaccttc agcctgcgcg acggcggcta ctacagctcc
    10201 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg
    10261 ggccccatgt tcgccttccg ccgcgtggag gaggatcaca gcaacaccga gctgggcatc
    10321 gtggagtacc agcacgcctt caagaccccg gatgcagatg ccggtgaaga aagagtttaa
    10381 acggccggcc gcggtcatag ctgtttcctg aacagatccc gggtggcatc cctgtgaccc
    10441 ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct
    10501 aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg
    10561 gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt
    10621 ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc
    10681 ctgggttcaa gcgattctcc
//
