LOCUS       sequence-47364-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SGC Empty backbone for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  TITLE     SGC Q3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET28-SBP-TEV
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..188
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             201..221
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(249..282)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        297..742
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 gacattccct ctgaaatatt ttgtttactt taagaaggag atataccatg catcatcacc
       61 atcaccatga attcatggac gaaaaaacca ccggttggcg tggtggtcac gttgttgaag
      121 gtctggctgg tgaactggaa cagctgcgtg ctcgtctgga acaccacccg cagggtcagc
      181 gtgaaccgag cagcggcaga gaaaacttgt atttccaggg ccatatgagt tctcctcctg
      241 aaagatccat aacttcgtat agcatacatt atacgaagtt atgcggccgc gacgtccaca
      301 tatacctgcc gttcactatt atttagtgaa atgagatatt atgatatttt ctgaattgtg
      361 attaaaaagg caactttatg cccatgcaac agaaactata aaaaatacag agaatgaaaa
      421 gaaacagata gattttttag ttctttaggc ccgtagtctg caaatccttt tatgattttc
      481 tatcaaacaa aagaggaaaa tagaccagtt gcaatccaaa cgagagtcta atagaatgag
      541 gtcgaaaagt aaatcgcgcg ggtttgttac tgataaagca ggcaagacct aaaatgtgta
      601 aagggcaaag tgtatacttt ggcgtcaccc cttacatatt ttaggtcttt ttttattgtg
      661 cgtaactaac ttgccatctt caaacaggag ggctggaaga agcagaccgc taacacagta
      721 cataaaaaag gagacatgaa cgatgaacat caaaaagttt gcaaaacaag caacagtatt
      781 aacctttact accgcactgc tggcaggagg cgcaactcaa gcgtttgcga aagaaacgaa
      841 ccaaagctta ggactcgatc accaaccaaa
//
