LOCUS       sequence-473711-        7864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  stable expression of p100 using virus infection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7864)
  AUTHORS   Busino L, Millman SE, Scotto L, Kyratsous CA, Basrur V, O'Connor O, 
            Hoffmann A, Elenitoba-Johnson KS, Pagano M
  TITLE     Fbxw7alpha- and GSK3-mediated degradation of p100 is a pro-survival 
            mechanism in multiple myeloma.
  JOURNAL   Nat Cell Biol. 2012 Mar 4;14(4):375-85. doi: 10.1038/ncb2463.
  PUBMED    22388891
REFERENCE   2  (bases 1 to 7864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Cell Biol"; date: "2012-03-4- 4"; volume: "14"; issue: "4"; 
            pages: "375-85"
COMMENT     Sequence Label: pBABE FLAG HA p100
FEATURES             Location/Qualifiers
     source          1..7864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(16..39)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(68..484)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(84..100)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(116..138)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    685..884
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             949..1418
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        1449..1553
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1554..1622,1623..2414)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1772..1791)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2574..3162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3063..3082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3316..3333
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             3463..3932
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(4153..4752)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4236..4256)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     4733..4752
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(4762..5091)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4776..4911
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5076..5096
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(7841..7864,1..3))
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(join(7841..7864,1..3))
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gtatcctcca tcgagcttgt catcgtcgtc cttgtagtcc atggttcatc gagcggagga
       61 tccggccggc gcctagagaa ggagtgaggg ctggataaag ggaggatcga ggcggggtcg
      121 aacgaggagg ttcaaggggg agagacgggg cggatggagg aagaggaggc ggaggcttag
      181 ggtgtacaaa gggcttgacc cagggagggg ggtcaaaagc caaggcttcc caggtcacga
      241 tgtaggggac ctggtctggg tgtccatgcg ggccaggtga aaagaccttg atcttaacct
      301 gggtgatgag gtctcggtta aaggtgccgt ctcgcggcca tccgacgtta aaggttggcc
      361 attctgcaga gcagaaggta acccaacgtc tcttcttgac atctaccgac tggttgtgag
      421 cgatccgctc gacatctttc cagtgaccta aggtcaaact taagggagtg gtaacagtct
      481 ggccctaatt ttcagacaaa tacagaaaca cagtcagaca gagacaacac agaacgatgc
      541 tgcagcgctg cagcagacaa gacgcgcggc ttcggttcca aaccgaaagc aaaaattcag
      601 acggaggcgg gaactgtttt aggttctcgt ctcctaccag aaccacatat cctgacgggg
      661 tcggattcca catcgactcc cttcctcagg tcgggccaca aaaacggccc ccaaagtccc
      721 tgggacgtct cccagggttg cggccgggtg ttcagaactc gtcagttcca ccacgggtcc
      781 gccagataca gagctagtta gctaactagt accgacgcag gcgcataaaa tcagtcatag
      841 acactagaca atcggacaga cacagataag ttgctggcca gcttgcctcc cggtggtggg
      901 tcggtggtcc ctgggcaggg gtctcccgat cccggacgag cccccaaatg aaagaccccc
      961 gctgacgggt agtcaatcac tcagaggaga ccctcccaag gaacagcgag accacaagtc
     1021 ggatgcaact gcaagagggt ttattggata cacgggtacc cgggcgactc agtcaatcgg
     1081 aggactggcg ccccgagtga ggggttgtgg gctcttttat tgagctcggg gagcagaagc
     1141 gcgcgaacag aagcgagaag cgaactgatt ggttagttca aataaggcac agggtcattt
     1201 caggtccttg gggcaccctg gaaacatctg atggttctct agaaactgct gagggctgga
     1261 ccgcatctgg ggaccatctg ttcttggccc tgagccgggg caggaactgc ttaccacaga
     1321 tatcctgttt ggcccatatt cagctgttcc atctgttctt ggccctgagc cggggcagga
     1381 actgcttacc acagatatcc tgtttggccc atattcaggc tgcaggtggc acttttcggg
     1441 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1501 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1561 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1621 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1681 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1741 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     1801 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1861 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1921 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1981 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2041 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     2101 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2161 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2221 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2281 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2341 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2401 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttgcggc
     2461 cgcaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2701 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcaact atggccattt aatgtaaata
     3421 cttaagaaaa aaaaccaaat taattttgat acatgctgca tgtgaaagac ccccgctgac
     3481 gggtagtcaa tcactcagag gagaccctcc caaggaacag cgagaccaca agtcggatgc
     3541 aactgcaaga gggtttattg gatacacggg tacccgggcg actcagtcaa tcggaggact
     3601 ggcgccccga gtgaggggtt gtgggctctt ttattgagct cggggagcag aagcgcgcga
     3661 acagaagcga gaagcgaact gattggttag ttcaaataag gcacagggtc atttcaggtc
     3721 cttggggcac cctggaaaca tctgatggtt ctctagaaac tgctgagggc tggaccgcat
     3781 ctggggacca tctgttcttg gccctgagcc ggggcaggaa ctgcttacca cagatatcct
     3841 gtttggccca tattcagctg ttccatctgt tcttggccct gagccggggc aggaactgct
     3901 taccacagat atcctgtttg gcccatattc agctgttcca tctgttcctg accttgatct
     3961 gaacttctct attctcagtt atgtattttt ccatgccttg caaaatggcg ttacttaagc
     4021 tagcttgcca aacctacagg tggggtcttt cattcccccc tttttctgga gactaaataa
     4081 aatcttttat tttatcgatg catggggtcg tgcgctcctt tcggtcgggc gctgcgggtc
     4141 gtggggcggg cgtcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg
     4201 cacctcgacg tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg
     4261 cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag
     4321 cacgacggcg ctgcccagac ccttgccctg gtggtcgggc gagacgccga cggtggccag
     4381 gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc
     4441 ggccagccgg gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc
     4501 cgcttcgacg ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca
     4561 caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg
     4621 ctcgatgtgg cggtccggat cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc
     4681 ggcgagggtg cgtacggccc tggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt
     4741 gtactcggtc atggtaagct ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta
     4801 cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag
     4861 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     4921 taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     4981 tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat
     5041 acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca cattccacag
     5101 ggtcgaccac tgtgctggcg aattctcagt gcacctgagg ctgggggtgc ccgtggcaga
     5161 gccctcctgg tggctcaggg ggtgggccca gcttctctgc ctcctgctcc actgactggc
     5221 tcccgtacgc actgtcttcc ttcacctctg ctgtgctggg cagcttgtct cgggtttctg
     5281 gacccctcag cagcctcact ccctcctcta ggcccatgtc agacagggcc tccagtagac
     5341 ctgccaggtc cccgccagcc agctcgtagc tgcgcaggag gctgccactg ggtgaggttg
     5401 tctgtcggta cgtgtctacc aggctgcgca gccccagacg ctctgccagc tctgcccagc
     5461 tgccctgggc ttctggcccg tctagcagct gctccaggtt ctgcagagct gtatcaccaa
     5521 gtgacagtcc cggccctgct gggctgggcg gggtcagggg tggctccatg gtgttctgag
     5581 cagcatttag cagcaaggtc ttcaccttgg tgctgcaagt gaggtcaaga ggcgtgtggc
     5641 cccggaagct gcttcgggtg tccttctcag gcccttcaga gtccgagtcg ctatcagagg
     5701 tagggggtga aggcagtggg cacaggggct cctcgttttc agcatggatg tcagcaccag
     5761 ccttcagaag gaggtgggtg agggtcgggt accccagtcc agctgccagg tgcaggggtg
     5821 tgtttcccgc aaaggtgcga gcgttcacgt tggcccggag cttggtgacc agatgggtga
     5881 ccaaccccag ctcctccatc tctgtggcta gatgcaaggc tgttcgtccc ccctgccgct
     5941 ctgtggcctc cacttcagcc ccactgtcca ccagcagatc caggcactca gggcttcggg
     6001 ctcggaccgc caggtgtact ggatacagtc cctcaaagtc aggcatatgc aacagctggg
     6061 gcacagcagg agctccactc tgaagcagtg cacgcagcag ctcaggagca ccagcgcctg
     6121 cccgcagcgc cagatgcatg gctgagtctc catgccgatc cagcagagct gggtctgcac
     6181 ctacccgcag cagaaagctc accacactcg tctgccccgt gatcaccgcc aggtgcaggg
     6241 gcgtctggtg caggtggttg gtgaggttga caacgccgag gtcctgggcg tggtggatga
     6301 catagactat ctgctcaatg acactggtct gcccgtggat gatggctagg tgcagtggtg
     6361 tgtctccgtt ctcgtcctgc gccgtcagca ggtggcgctg tcccgccagc agcgcgcgcg
     6421 cgtccgcggt gacgccgtag tcgagtaggg ctcgggcgct gcgctgcgcc aggccgaaca
     6481 ggcgcgcgtt gtactctcga gctcgctgca gcatctccgg ggcctgcggc tcgcactggg
     6541 gggtcctgga gggggcgctc ggctccgcgg cttcctcccc ggagtccctg ctgggcaccg
     6601 tggcggccat ctgcgccccg cccccgcctc ccgggtagca gcccatgggg cccgcgccgg
     6661 actggtaggg gctgtaggcc agggaggagg ggaagaaacc gaggctgcca cctcctccag
     6721 ctcctccgta gcccccggct gcacccccag agcctccacc catgtgggag ccacccccga
     6781 agggctggga gaaggtgggc aaggccttcc tccgcttccg ctgcacctct tccttgtctt
     6841 ccaccagagg gtaataggtg aactgtttgg aatcagacac gtcccctcct cgcttgcgtt
     6901 tcagttgcag aaacactgtt acaggccgct caatcttcat cttgtgatag gggggtgtcc
     6961 ggaacacaat ggcatactgt ttatgcacat ctgtgggaga gaagtcccca aaggcctgcc
     7021 atccattctc atcatcctca tagaaccgaa cctcaatgtc atctttctgc accttgtcac
     7081 aaagcagata aacttcatct ccaccccgca cagagcctgc tgtcttgtcc attcgagaaa
     7141 tcttcaggtt tgatgccccc ggagatttgc tgtcatggat gggctgggag atgactggct
     7201 tcaggggcag ggagaaggag ccatcactgg ctctaaggaa ggcagagaag cgcagccgca
     7261 ctatactcag atccatcacc ttcttcagtt ctttggcctc ttgctccagc tcccgctgct
     7321 cggcctccgt aaggccctgg ggcctagagc ggagccgctg cctctgaagt ttttgtatca
     7381 tagtccccat catgttcttc ttagtcacat gcaggacacc caggttgtta aattgggcag
     7441 tcatgtcctt gggccccaca gaaacggcgc agatccccag ctccgagcat tgcttgccca
     7501 ccagactgtg ggcatgagca cgaggtgggt cactgtgtgt taccaggtcc acctcgatct
     7561 tggctggtcc ctcgtagtta cagatcttga cggtgggata ggtctttcgg cccttctcac
     7621 tggaggcacc gggcagtcct ccatgggagg ggccttcaca gccatatcga aatcggaagc
     7681 ctctctgctt aggctgttcc acgatcacca ggtaggggcc atcagctgtt tctggggctg
     7741 gctccttggg ttccacaatg gaggagttca atttgaaatc atcatattca ataataccat
     7801 ccagacctgg gttgtagcaa ctctccatgg atcttccttc ggcgtagtcg ggcacgtcgt
     7861 aggg
//
