LOCUS       sequence-473791-        8807 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lysosome-targeted FRET-based biosensor for monitoring diacylglycerol
            (DAG) dynamics in living cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8807)
  AUTHORS   Su Q, Zhang J, Lin W, Zhang JF, Newton AC, Mehta S, Yang J, Zhang J
  TITLE     Sensitive fluorescent biosensor reveals differential subcellular 
            regulation of PKC.
  JOURNAL   Nat Chem Biol. 2024 Oct 11. doi: 10.1038/s41589-024-01758-3.
  PUBMED    39394268
REFERENCE   2  (bases 1 to 8807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Oct 11. doi: 10.1038/s41589-024-01758-3."
COMMENT     Sequence Label: pcDNA3-Lyso-Digda
FEATURES             Location/Qualifiers
     source          1..8807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      901..910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             922..939
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             943..975
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(979..987,988..1002)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             join(1009..1011,1012..1014,1015..1725)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2287..2289,2290..2292,2293..2805)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     regulatory      3089..3098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3091..3174,3175..4344)
                     /codon_start=1
                     /product="human lysosome-associated membrane glycoprotein 
                     1"
                     /label=LAMP1
                     /translation="MAAPGSARRPLLLLLLLLLLGLMHCASAAMFMVKNGNGTACIMAN
                     FSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNF
                     TRNATRYSVQLMSFAYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHM
                     NNVTVTPHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKY
                     NVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSE
                     GTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNA
                     EEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSMLIPIAVGGALAGLVLIV
                     LIAYLVGRKRSHAGYQTI"
     promoter        complement(4360..4378)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4361..4378)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4398..4415)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4404..4628
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4674..5102
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4761..4780)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4971..4992
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5111..5131)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5116..5445
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5296..5431
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5358..5377
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5512..6306
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5566..5585)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6176..6195
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6480..6601
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6517..6536)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6571..6590
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6650..6666)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6650..6666)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6663..6685)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6674..6690
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6698..6704,6705..6722,6723..6728))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6743..6764
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6881..6898)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7052..7640)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7132..7151)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7811..8602,8603..8671))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8434..8453
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8672..8776)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcggcc
      901 gccaccatgg tgcggggttc tcatcatcat catcatcatg gtatggctag catgactggt
      961 ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatcccat ggtgagcaag
     1021 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1081 ggccacaggt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1141 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1201 ctgacctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
     1261 ttcaagtccg ccatgcccga aggctacgtc caggagcgta ccatcttctt caaggacgac
     1321 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1381 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcataa gctggagtac
     1441 aacgccatca gtgacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
     1501 cacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1561 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1621 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1681 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagctcga ggaagccgca
     1741 gctagagagg ctgccgcaag ggaagcagcc gctcgggagg ctgccgcaag ggaagcagcc
     1801 gctagggagg ccgctgcaag atccggacac aaattcaccg cccgcttctt caagcagccc
     1861 accttctgca gccactgcac cgacttcatc tggggctttg ggaagcaggg attccagtgt
     1921 caagtttgct gctttgtagt gcacaagcgg tgccatgagt ttgtcacatt ctcctgccct
     1981 ggtgctgaca agggcccagc ctctgatgac ccccggagca aacacaagtt caagatccac
     2041 acgtactcca gccccacgtt ctgtgaccac tgtggatccc tgctgtacgg gctcatccac
     2101 caggggatga aatgcgacac ctgcatgatg aacgtgcaca agcgctgcgt gatgaacgtc
     2161 ccaagtctat gcggcggtac cgaggctgcc gcaagagaag ccgctgcaag ggaggctgca
     2221 gctcggggcg gagaagccgc agcaagggag gctgccgcaa gggaagccgc agctagaggc
     2281 ggccgcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     2341 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     2401 acctacggca agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg
     2461 cccaccctcg tgaccaccct gggctacggc ctgcagtgct tcgcccgcta ccccgaccac
     2521 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     2581 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     2641 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     2701 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcaccgc cgacaagcag
     2761 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag
     2821 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     2881 aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     2941 atggtcctgc tggagttcgt gaccgccgcc tctagtgaag ctgcagcaag agaggccgct
     3001 gctagagaag cagccgctcg ggaggccgct gcaagggaag cagccgcaag agaggctgcc
     3061 gcaagggagg ccgctgctag gtctagaacc atggcggcgc ccggcagcgc ccggcgaccc
     3121 ctgctgctgc tactgctgtt gctgctgctc ggcctcatgc attgtgcgtc agcagcaatg
     3181 tttatggtga aaaatggcaa cgggaccgcg tgcataatgg ccaacttctc tgctgccttc
     3241 tcagtgaact acgacaccaa gagtggccct aagaacatga cctttgacct gccatcagat
     3301 gccacagtgg tgctcaaccg cagctcctgt ggaaaagaga acacttctga ccccagtctc
     3361 gtgattgctt ttggaagagg acatacactc actctcaatt tcacgagaaa tgcaacacgt
     3421 tacagcgtcc agctcatgag ttttgcttat aacttgtcag acacacacct tttccccaat
     3481 gcgagctcca aagaaatcaa gactgtggaa tctataactg acatcagggc agatatagat
     3541 aaaaaataca gatgtgttag tggcacccag gtccacatga acaacgtgac cgtaacgccc
     3601 catgatgcca ccatccaggc gtacctttcc aacagcagct tcagcagggg agagacacgc
     3661 tgtgaacaag acaggccttc cccaaccaca gcgccccctg cgccacccag cccctcgccc
     3721 tcacccgtgc ccaagagccc ctctgtggac aagtacaacg tgagcggcac caacgggacc
     3781 tgcctgctgg ccagcatggg gctgcagctg aacctcacct atgagaggaa ggacaacacg
     3841 acggtgacaa ggcttctcaa catcaacccc aacaagacct cggccagcgg gagctgcggc
     3901 gcccacctgg tgactctgga gctgcacagc gagggcacca ccgtcctgct cttccagttc
     3961 gggatgaatg caagttctag ccggtttttc ctacaaggaa tccagttgaa tacaattctt
     4021 cctgacgcca gagaccctgc ctttaaagct gccaacggct ccctgcgagc gctgcaggcc
     4081 acagtcggca attcctacaa gtgcaacgcg gaggagcacg tccgtgtcac gaaggcgttt
     4141 tcagtcaata tattcaaagt gtgggtccag gctttcaagg tggaaggtgg ccagtttggc
     4201 tctgtggagg agtgtctgct ggacgagaac agcatgctga tccccatcgc tgtgggtggt
     4261 gccctggcgg ggctggtcct catcgtcctc atcgcctacc tcgtcggcag gaagaggagt
     4321 cacgcaggct accagactat ctaagaattc gggccctatt ctatagtgtc acctaaatgc
     4381 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     4441 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     4501 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     4561 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     4621 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     4681 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4741 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4801 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4861 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4921 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4981 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     5041 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     5101 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     5161 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     5221 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     5281 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     5341 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     5401 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     5461 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     5521 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     5581 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     5641 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     5701 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     5761 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     5821 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     5881 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     5941 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     6001 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     6061 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     6121 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     6181 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     6241 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     6301 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     6361 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     6421 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     6481 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6541 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6601 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     6661 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6721 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6781 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6841 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6901 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6961 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7021 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7081 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7141 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7201 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7261 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7321 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7381 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7441 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     7501 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7561 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7621 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7681 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7741 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7801 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7861 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7921 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7981 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8041 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8101 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     8161 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8221 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8281 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     8341 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8401 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8461 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8521 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8581 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8641 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8701 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8761 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
