LOCUS       sequence-473827-        6384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF208 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 6384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF208
FEATURES             Location/Qualifiers
     source          1..6384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(3842..3941)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3958..3977)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3959..3977)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4100..4119)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4111..4920
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4178..4197)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5067..5655
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5556..5575
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5809..5826
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5985..6012)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6104..6190)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     6239..6261
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6253..6270
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6254..6270
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(6286..6384,1)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgggttctt tgactttcag agatgttgca attgaatttt ccctagaaga gtggcaatgc
       61 cttgacacag cccaacaaaa cctataccgg aatgtaatgc ttgaaaatta caggaacctt
      121 gttttccttg gaatcgcggc ttttaagcca gacctgataa ttttcctgga agaaggaaaa
      181 gaatcctgga acatgaagag acatgaaatg gtagaagaat cacctgtgat ctgttcccat
      241 tttgcccaag acctgtggcc agaacaagga attgaagatt ctttccaaaa agtgatcctg
      301 aggagatatg aaaagtgtgg ccatgaaaac ctccacctaa agattgggta cacgaatgta
      361 gatgagtgta aggtccataa agagggatat aataagctga accaatccct aaccacgacc
      421 cagagtaaag ttttccagag aggaaaatac gcaaacgttt tccataagtg ttcgaacagt
      481 aaccgccata aaatcagaca tactgggaag aaacacctcc agtgtaaaga gtatgtcaga
      541 agtttctgta tgctcagcca cctcagccaa cataaaagaa tatacaccag agagaacagc
      601 tataagtgtg aagaaggcgg aaaagcattt aactggagca gcaccctcac atattataaa
      661 tcggcccaca ccggagaaaa gccatacaga tgtaaagagt gcggaaaggc cttttcgaag
      721 ttttcgattt taacgaaaca taaagtgatc cacacaggtg agaagagcta caagtgcgaa
      781 gaatgtggaa aggcatttaa ccaatcggcg atcctgacta aacataagat tatccatact
      841 ggggaaaagc caaataagtg tgaagagtgt gggaaggctt tttcaaaagt aagcaccctg
      901 acaacccata aggccatcca tgcaggagaa aagccatata aatgtaaaga gtgtggaaag
      961 gcattttcaa aggtatccac cctgattacc cataaggcta tccatgctgg agaaaagccg
     1021 tacaaatgta aggaatgcgg gaaggctttc agtaaattca gcatcctgac aaaacataag
     1081 gttatacata ccggtgagaa accgtataag tgtgaagagt gtgggaaagc atataaatgg
     1141 ccgagcaccc tatcgtacca taaaaagatt catacaggtg agaaaccata caagtgtgaa
     1201 gagtgtggga agggtttttc gatgttttca atccttacga aacacgaggt gatacacaca
     1261 ggagagaaac catataagtg cgaagaatgt ggtaaagctt ttaactggag cagtaacctg
     1321 atggagcata agaaaatcca cactggggag acgccatata agtgtgaaga gtgtgggaag
     1381 gggttttcca tgttcagtat tttgacaaag cataaagtca tccataatgg tgagaagccg
     1441 tataagtgtg aagagtgtga taaggccacc catgcgggtg aaaaaccata taagtgtgaa
     1501 gagtgtggaa aggcttttaa ctggagcagt aacctaatgg aacataagag gatccatacg
     1561 ggagaaaagc catataagtg tgaagagtgt gggaaaagtt tcagcacttt ttcgattctg
     1621 acgaagcaca aagtgataca tacaggtgag aagccatata agtgtgaaga gtgtggaaag
     1681 gcatataagt ggagttccac cctgtcatac cataagaaga tccacactgt agaaaagcca
     1741 tataagtgtg aagagtgtgg aaaggctttt aaccagagcg cgatcctaat caaacataaa
     1801 aggatccaca ccggtgaaaa gccatataag tgtgaagagt gtgggaagac attcagtaaa
     1861 gtatcaactt tgaccaccca taaagcgatc catgccggag agaagccata caagtgtaaa
     1921 gaatgcggaa agacttttat caaagtttcg acattgacca cacataaggc aatacatgcg
     1981 ggagagaagc catataagtg caaagagtgt ggtaaggctt tctcgaaatt ttctatccta
     2041 acgaaacata aagttatcca cacgggtgag aagccgtata agtgtgaaga gtgtggaaaa
     2101 gcttttaact ggagttcgaa cctcatggaa cataagagga tacacaccgg tgaaaagcca
     2161 tataagtgtg aagagtgtgg gaagagtttc agtactttca gtgtcctcac gaaacacaaa
     2221 gtaatccata caggtgaaaa gccatataag tgcgaagaat gtgggaaggc gtataagtgg
     2281 agttctaccc tatcatacca caaaaaaatc cacactgttg agaagccata taagtgtgaa
     2341 gagtgtggga aggcttttaa cagaagtgcg atcctgataa agcataaacg aatccacact
     2401 gatgaaaagc catataagtg tgaagagtgc gggaagactt tttctaaagt gagcactttg
     2461 acgacccata aggcgatcca cgcaggagaa aagccatata agtgtaagga gtgcgggaaa
     2521 gctttttcga aattcagtat tctcacgaag cataaagtaa tacataccgg agagaagccg
     2581 tataagtgcg aagaatgtgg aaaggcatat aagtggccga gtaccctttc gtaccataag
     2641 aaaatccata ctggagagaa gccatataag tgtgaagagt gtgggaaagg tttcagcatg
     2701 ttttcgatcc tcacaaaaca tgaagtgatc cacaccggtg aaaagcccta taaatgtgag
     2761 gaatgtggaa aggcattcag ctggctgagt gttttttcga aacataagaa gacacatgct
     2821 ggtgagaaat tctataagtg tgaggcctgt gggaaggcat ataagagcag cagcactctg
     2881 agttaccata agaaaataca cactgaagag aagccatata aatatgagga gtgtggaaag
     2941 ggattcagta cattcagcat cctcacgaag cataaggtga tccatactgg tgagaagcca
     3001 tataagtgtg aagagtgtgg gaaggccttt aactggagtt caaatttgat ggaacataaa
     3061 aagatacaca ctggagaaac gccgtataag tgtgaagagt gtgataaagc gttttcctgg
     3121 ccaagcagcc taacagaaca taaagccaca catgccggtg aaaagccata taagtgtgaa
     3181 gaatgtggaa aggctttttc gtggccatcg agattaacag agcataaagc tacccacgct
     3241 ggtgaagaac catataagtg tgaagagtgt ggaaaggctt ttaactggag cagtaatctg
     3301 atggaacata agagaatcca taccggggag aaaccatata agtgtgaaga gtgtgggaaa
     3361 tctttcagca cattcagtat cctgacgaaa cataaggtca tccatacagg agaaaagcca
     3421 tacaaatgcg aagagtgtgg aaaggcatat aaatggagca gtaccctcag ctaccataag
     3481 aagattcaca ctgttgaaaa gccatataag tgtgaagagt gcgggaaagg gtttgtaatg
     3541 ttcagcatat tggcgaaaca taaggtaatc cacactggcg aaaaactgta taaatgtgaa
     3601 gagtgcggta aagcatataa gtggccttcg accctgagat accataaaaa gatccataca
     3661 ggggagaagc catataagtg tgaagagtgt gggaaggctt tttccacttt cagcattttg
     3721 acgaaacata aagtgatcca cactggtgaa aagccatata agtgtgagga atgcggtaag
     3781 gcgttttcgt ggctatcggt tttcagtaag cataaaaaaa ttcacaccgg ggaaaaatta
     3841 tacccaactt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
     3901 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     3961 tatagtgagt cgtattacat ggtcatagct gtttcttggc agctctggcc cgtgtctcaa
     4021 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     4081 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     4141 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     4201 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     4261 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     4321 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     4381 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     4441 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     4501 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     4561 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     4621 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     4681 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     4741 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     4801 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     4861 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     4921 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     4981 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     5041 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5101 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5161 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     5221 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5281 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5341 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5401 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5461 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5521 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5581 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5641 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5701 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     5761 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     5821 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     5881 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     5941 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     6001 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     6061 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     6121 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     6181 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     6241 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccccaaat aatgatttta
     6301 ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt ttttataatg
     6361 ccaactttgt acaaaaaagt tggc
//
