LOCUS       sequence-473829-        3822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF736 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF736
FEATURES             Location/Qualifiers
     source          1..3822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1280..1379)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1396..1415)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1397..1415)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1538..1557)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1549..2358
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1616..1635)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2505..3093
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2994..3013
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3247..3264
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3423..3450)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3542..3628)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3677..3699
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3691..3708
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3692..3708
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(3724..3822,1)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgggtgtcc tgactttcag agatgtggct gtagaatttt cgccagaaga gtgggaatgc
       61 ctagattcag cccaacaaag attatacaga gatgtaatgc ttgaaaatta tgggaacctt
      121 gtcagccttg gcctggcgat ttttaagcca gacctaatga catgtttaga acaaagaaaa
      181 gagccatgga aagtaaagag acaagaagct gtagcaaaac acccggctgg aagtttccat
      241 tttacagcag aaatcctccc agaccatgat ataaaagatt ctttccagaa agtgatcctg
      301 agaaaatatg ggagctgtga cctaaataac ctccacctga aaaaagacta ccagagtgtt
      361 ggaaactgta aaggccaaaa gagttcatat aatggcctcc accaatgttt atcggcaacc
      421 cattcaaaga cctgccaatg taataagtgc ggcagaggtt tccagctctg ttcaattttt
      481 actgaacata aagatatttt ttccagagag aagtgccata aatgtgagga gtgcgggaaa
      541 gactgcagac ttttttcgga tttcaccaga cataagaaga tccatactgt tgagaggtgc
      601 tataagtgtg aagagtgtgg gaaagctttt aagaaatttt cgaacctcac agaacataag
      661 agagtccata caggagaaaa accgtataag tgtgaaggct gcggaaaaac tttcacgtgt
      721 tcatcgaccc ttgtaaaaca taagagaaac catactggtg acaggccata taagtgtgaa
      781 gagtgcggaa aggcttttaa atgtttcagt gatttgacaa accataagag gatccacacc
      841 ggtgaaaaac catataagtg tgaagagtgt aataaggcat acaggtggtt cagtgacctt
      901 gcaaaacata aaataatcca taccggtgat aagccataca cctgtaatga atgtggaaag
      961 gcatttaagt ggttttcggc cctttcgaaa cataagagaa tccacacagg tgaaaagcca
     1021 tatatctgtg aagagtgtgg aaaagcattc acgaggagca gcaccctttt taaccataaa
     1081 aggatccata tggaagaacg cccatataag tgtgaggaat gcagtaagac ttttaagtgt
     1141 ttttctgacc tcacaaacca caagaggata cacactggtg agaagccata taaatgtgaa
     1201 gaatgtggga aggccagttc ctggttttcc catttaataa gacataaaag aatacacacg
     1261 agagaaaagc tccataagtg cccaactttc ttgtacaaag ttggcattat aagaaagcat
     1321 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc
     1381 catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcttggcag
     1441 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     1501 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
     1561 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     1621 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
     1681 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     1741 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     1801 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca
     1861 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     1921 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     1981 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     2041 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     2101 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     2161 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     2221 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     2281 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     2341 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg
     2401 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg
     2461 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2521 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2581 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2641 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     2701 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     2761 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     2821 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2881 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2941 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3001 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3061 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3121 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3181 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     3241 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     3301 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     3361 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
     3421 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
     3481 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
     3541 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     3601 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
     3661 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
     3721 ccccaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag
     3781 caatgctttt ttataatgcc aactttgtac aaaaaagttg gc
//
