LOCUS       sequence-473888-        4082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vitro transcription of bovine arrestin-3 short isoform.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Ahmed MR, Zhan X, Song X, Kook S, Gurevich VV, Gurevich EV
  TITLE     Ubiquitin ligase parkin promotes Mdm2-arrestin interaction but 
            inhibits arrestin ubiquitination.
  JOURNAL   Biochemistry. 2011 May 10;50(18):3749-63. doi: 10.1021/bi200175q. 
            Epub 2011 Apr 12.
  PUBMED    21466165
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi200175q";
            journalName: "Biochemistry"; date: "2011-05-10- 10"; volume: "50"; 
            issue: "18"; pages: "3749-63"
COMMENT     Sequence Label: BtA3N
FEATURES             Location/Qualifiers
     source          1..4082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1301..1320)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1302..1320)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1401..1418)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1572..2160)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1652..1671)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2331..3122,3123..3191))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2954..2973
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3192..3296)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3359..3377
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3415..3437)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3537..3556
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3770..3789
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(4079..4082,1..15)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     join(4079..4082,1..14)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 aggtgacact atagaataca cggaattctt ttttttttaa accaccatgg gggagaaacc
       61 cgggaccagg gtcttcaaga aatcgagtcc taactgcaag ctcaccgtgt acctaggcaa
      121 gcgggacttt gtggaccacc tggacaaagt ggaccctgta gatggtgtgg tgctagtgga
      181 tccggactac ttgaaggacc gcaaagtgtt tgtgaccctc acgtgcgcct ttcgctatgg
      241 ccgcgaagac ctggacgtcc tgggcttgtc cttccgcaaa gacctgttca tcgccaacta
      301 ccaggccttc cctccaacac ctaacccacc tagaccacct acgcgtctgc aggagcggct
      361 gctgaggaag ctgggccagc atgcccaccc cttttttttc acaatacccc agaacctgcc
      421 ctgctccgtc accctacagc caggcccaga ggatacgggg aaggcttgcg gggtcgactt
      481 tgagattcga gccttctgcg ccaaatcact agaagagaaa agccacaaac ggaactccgt
      541 acgtctggtg atccgaaagg tgcagtttgc cccagagaaa ccagggcccc agccttcagc
      601 tgaaaccaca cgccacttcc tcatgtctga ccggtccctg caccttgagg cttcactgga
      661 caaggagctg tactaccacg gggagcccct caatgtcaat gtccacgtca ccaataactc
      721 caccaagact gtcaagaaga tcaaagtctc tgtgagacag tacgccgaca tctgcctctt
      781 cagcaccgcc cagtacaagt gccccgtggc tcaggtcgaa caagatgacc aggtgtcgcc
      841 cagttccacg ttctgtaagg tgtacaccat aaccccgctg ctcagcaaca accgggagaa
      901 gcgcggcctc gctctagatg ggaagctcaa gcacgaggac accaacctgg cctcgagcac
      961 cattgtgaag gagggtgcca acaaggaggt gctggggata ctggtgtcct acagggtcaa
     1021 ggtgaagctg gtggtgtcac gaggcgggga tgtctcagtg gagctgccat ttgttctcat
     1081 gcaccccaag ccccatgatc acatcgccct tcccaggccc cagtcagctg ttcctgaaac
     1141 agatgccccc gtggacacca acctcattga atttgaaacc aactatgcca cagacgacga
     1201 catcgtgttc gaagactttg cccggctgcg gctcaagggg ctgaaggacg aggactatga
     1261 cgaccagttc tgtatcgatt agggctggaa gcttgggtct ccctatagtg agtcgtatta
     1321 atttcgataa gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     1381 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     1441 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     1501 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1561 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1621 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1681 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1741 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1801 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1861 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1921 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1981 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     2041 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2101 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2161 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2221 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2281 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     2341 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     2401 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     2461 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     2521 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     2581 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     2641 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     2701 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     2761 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     2821 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     2881 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     2941 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     3001 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     3061 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     3121 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     3181 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3241 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     3301 tttccccgaa aagtgccacc tgtaagaaac cattattatc atgacattaa cctataaaaa
     3361 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     3421 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     3481 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     3541 atcagagcag attgtactga gagtgcacca ttcgacgctc tcccttatgc gactcctgca
     3601 ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg
     3661 catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc
     3721 cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg
     3781 cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg
     3841 cgtagaggat ctggctagcg atgaccctgc tgattggttc gctgaccatt tccgggtgcg
     3901 ggacggcgtt accagaaact cagaaggttc gtccaaccaa accgactctg acggcagttt
     3961 acgagagaga tgatagggtc tgcttcagta agccagatgc tacacaatta ggcttgtaca
     4021 tattgtcgtt agaacgcggc tacaattaat acataacctt atgtatcata cacatacgat
     4081 tt
//
