LOCUS       sequence-473889-        9083 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SspB-PPM1H in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   Wang X, Xu P, Bentley-DeSousa A, Hancock-Cerutti W, Cai S, Johnson 
            BT, Tonelli F, Shao L, Talaia G, Alessi DR, Ferguson SM, De Camilli 
            P
  TITLE     The bridge-like lipid transport protein VPS13C/PARK23 mediates 
            ER-lysosome contacts following lysosome damage.
  JOURNAL   Nat Cell Biol. 2025 May;27(5):776-789. doi: 
            10.1038/s41556-025-01653-6. Epub 2025 Apr 10.
  PUBMED    40211074
REFERENCE   2  (bases 1 to 9083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-025-01653-6"; journalName: "Nat Cell Biol"; date: 
            "2025-05"; volume: "27"; issue: "5"; pages: "776-789"
COMMENT     Sequence Label: Halo-SspB-PPM1H
FEATURES             Location/Qualifiers
     source          1..9083
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     852..872
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             931..996
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             997..1020
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             1024..1914
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             1936..2265
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     polyA_signal    3842..4464
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4041..4060)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(4488..4508)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4506..4803
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4857..5651
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4911..4930)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5521..5540
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6303..6437
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6340..6359)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6394..6413
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6442..6461)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6443..6461)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6475..6491)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6475..6491)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6488..6510)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6499..6515
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6523..6529,6530..6547,6548..6553))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6568..6589
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6706..6723)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6877..7465)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6957..6976)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7636..8427,8428..8496))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8259..8278
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8497..8601)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8628..9056
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8715..8734)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8925..8946
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attaaccatg gactacaaag accatgacgg tgattataaa
      961 gatcatgaca tcgattacaa ggatgacgat gacaagctgg aagttctgtt ccaggggccc
     1021 cttatggcag aaatcggtac tggctttcca ttcgaccccc attatgtgga agtcctgggc
     1081 gagcgcatgc actacgtcga tgttggtccg cgcgatggca cccctgtgct gttcctgcac
     1141 ggtaacccga cctcctccta cgtgtggcgc aacatcatcc cgcatgttgc accgacccat
     1201 cgctgcattg ctccagacct gatcggtatg ggcaaatccg acaaaccaga cctgggttat
     1261 ttcttcgacg accacgtccg cttcatggat gccttcatcg aagccctggg tctggaagag
     1321 gtcgtcctgg tcattcacga ctggggctcc gctctgggtt tccactgggc caagcgcaat
     1381 ccagagcgcg tcaaaggtat tgcatttatg gagttcatcc gccctatccc gacctgggac
     1441 gaatggccag aatttgcccg cgagaccttc caggccttcc gcaccaccga cgtcggccgc
     1501 aagctgatca tcgatcagaa cgtttttatc gagggtacgc tgccgatggg tgtcgtccgc
     1561 ccgctgactg aagtcgagat ggaccattac cgcgagccgt tcctgaatcc tgttgaccgc
     1621 gagccactgt ggcgcttccc aaacgagctg ccaatcgccg gtgagccagc gaacatcgtc
     1681 gcgctggtcg aagaatacat ggactggctg caccagtccc ctgtcccgaa gctgctgttc
     1741 tggggcaccc caggcgttct gatcccaccg gccgaagccg ctcgcctggc caaaagcctg
     1801 cctaactgca aggctgtgga catcggcccg ggtctgaatc tgctgcaaga agacaacccg
     1861 gacctgatcg gcagcgagat cgcgcgctgg ctgtcgacgc tcgagatttc cggcggcggt
     1921 ggaggttcag cggccagctc cccgaaacgc cctaagctgc tgcgtgaata ttacgattgg
     1981 ctggttgata acagctttac cccatatctg gtggtggatg ccacatacct gggcgtgaac
     2041 gtgcccgtgg agtatgtgaa agacggtcag atcgtgctga atctgtctgc aagtgcgacc
     2101 ggcaacctgc aactgacaaa tgattttatc cagttcaacg cccgctttaa gggcgtgtct
     2161 cgtgaactgt atatcccgat gggtgccgct ctggccattt acgctcgcga gaacggcgat
     2221 ggtgtgatgt tcgaaccaga agaaatctat gacgagctga atattgcggc cgcgggcggt
     2281 ggaggttcaa tgctcactcg agtgaaatct gccgtggcca atttcatggg cggcatcatg
     2341 gctggcagct caggctccga gcacggcggc ggcagctgcg gaggctcgga cctgcccctg
     2401 cgtttcccct acgggcggcc agagttcctg gggctgtctc aggacgaggt ggagtgcagc
     2461 gccgaccaca tcgcccgccc catcctcatc ctcaaggaga ctcggcggct gccctgggcc
     2521 actggctacg cagaggttat caatgccggg aagagcacac acaatgaaga ccaagccagc
     2581 tgtgaggtgc tcactgtgaa gaagaaggca ggggccgtga cctcaacccc aaacaggaac
     2641 tcatccaaga gacggtcctc ccttcccaat ggggaagggc tgcagctgaa ggagaactcg
     2701 gaatccgagg gtgtttcctg ccactattgg tcgctgtttg acgggcacgc ggggtccggg
     2761 gccgcggtgg tggcgtcacg cctgctgcag caccacatca cggagcagct gcaggacatc
     2821 gtggacatcc tgaagaactc cgccgtcctg ccccctacct gcctggggga ggagcctgag
     2881 aacacgcccg ccaacagccg gactctgacc cgggcagcct ccctgcgcgg aggggtgggg
     2941 gccccgggct cccccagcac gccccccaca cgcttcttta ccgagaagaa gattccccat
     3001 gagtgcctgg tcatcggagc gcttgaaagt gcattcaagg aaatggacct acagatagaa
     3061 cgagagagga gttcatataa tatatctggt ggctgcacgg ccctcattgt gatttgcctt
     3121 ttggggaagc tgtatgttgc aaatgctggg gatagcaggg ccataatcat cagaaatgga
     3181 gaaattatcc ccatgtcttc agaatttacc cccgagacgg agcgccagcg acttcagtac
     3241 ctggcattca tgcagcctca cttgctggga aatgagttca cacatttgga gtttccaagg
     3301 agagtacaga gaaaggagct tggaaagaag atgctctaca gggactttaa tatgacaggc
     3361 tgggcataca aaaccattga ggatgaggac ttgaagttcc cccttatata tggagaaggc
     3421 aagaaggccc gggtaatggc aactattgga gtgaccaggg gacttgggga ccatgacctg
     3481 aaggtgcatg actccaacat ctacattaaa ccattcttgt cttcagctcc agaggtaaga
     3541 atctacgatc tttcaaaata tgatcatgga tcagatgatg tgctgatctt ggccactgat
     3601 ggactctggg acgttttatc aaatgaagaa gtagcagaag caatcactca gtttcttcct
     3661 aactgtgatc cagatgatcc tcacaggtac acactggcag ctcaggacct ggtgatgcgt
     3721 gcccggggtg tgctgaagga cagaggatgg cggatatcta atgaccgact gggctcagga
     3781 gacgacattt ctgtatatgt cattccttta atacatggaa acaagctgtc atgaggatcc
     3841 cgggtggcat ccctgtgacc cctccccagt gcctctccgg gccctggaag ttgccactcc
     3901 agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc
     3961 cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac
     4021 aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg
     4081 gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt
     4141 gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg
     4201 gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc
     4261 ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct
     4321 gattttaaaa taactatacc agcaggagga cgtccagaca cagcataggc tacctggcca
     4381 tgcccaaccg gtgggacatt tgagttgctt gcttggcact gtcctctcat gcgttgggtc
     4441 cactcagtag atgcctgttg aattgggtac gcggccagct tggctgtgga atgtgtgtca
     4501 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4561 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4621 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     4681 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4741 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     4801 tggaggaatt gatcagcttg ggatctgatc aagagacagg atgaggatcg tttcgcatga
     4861 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     4921 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     4981 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     5041 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     5101 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     5161 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     5221 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     5281 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     5341 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     5401 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     5461 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     5521 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     5581 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     5641 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     5701 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     5761 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     5821 ccccgggctc gatcccctcg cgagttggtt cagctgctgc ctgaggctgg acgacctcgc
     5881 ggagttctac cggcagtgca aatccgtcgg catccaggaa accagcagcg gctatccgcg
     5941 catccatgcc cccgaactgc aggagtgggg aggcacgatg gccgctttgg tcgacccgga
     6001 cgggacgctc ctgcgcctga tacagaacga attgcttgca ggcatctcat gagtgtgtct
     6061 tcccgttttc cgcctgaggt cactgcgtgg atggagcgct ggcgcctgct gcgcgacggc
     6121 gagctgctca ccacccactc gccaagctgg aaccgtaaaa aggccgcgtt gctggcgttt
     6181 ttccataggc tccgccgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     6241 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     6301 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     6361 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6421 cttatcatgt ctggatcaat tccctatagt gagtcgtatt aaattcgtaa tcatgtcata
     6481 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6541 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6601 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6661 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6721 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6781 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6841 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6901 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6961 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7021 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7081 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7141 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7201 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7261 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7321 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7381 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7441 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7501 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7561 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7621 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7681 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7741 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7801 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7861 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7921 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7981 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8041 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8101 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8161 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8221 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8281 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8341 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8401 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8461 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8521 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8581 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag
     8641 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     8701 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     8761 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     8821 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     8881 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     8941 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     9001 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     9061 caaaatatta acgcttacaa ttt
//
