LOCUS       sequence-473895-        4076 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vitro transcription of mutant bovine arrestin-3 short isoform.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Ahmed MR, Zhan X, Song X, Kook S, Gurevich VV, Gurevich EV
  TITLE     Ubiquitin ligase parkin promotes Mdm2-arrestin interaction but 
            inhibits arrestin ubiquitination.
  JOURNAL   Biochemistry. 2011 May 10;50(18):3749-63. doi: 10.1021/bi200175q. 
            Epub 2011 Apr 12.
  PUBMED    21466165
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi200175q";
            journalName: "Biochemistry"; date: "2011-05-10- 10"; volume: "50"; 
            issue: "18"; pages: "3749-63"
COMMENT     Sequence Label: BtA3CL
FEATURES             Location/Qualifiers
     source          1..4076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1295..1314)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1296..1314)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1395..1412)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1566..2154)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1646..1665)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2325..3116,3117..3185))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2948..2967
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3186..3290)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3353..3371
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3409..3431)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3531..3550
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3764..3783
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(4073..4076,1..15)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     join(4073..4076,1..14)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 aggtgacact atagaataca cggaattctt ttttttttaa accaccatgg gggagaaacc
       61 cgggaccagg gtcttcaaga aatcgagtcc taacagcaag ctcaccgtgt acctaggcaa
      121 gcgggacttt gtggaccacc tggacaaagt ggaccctgta gatggtgtgg tgctagtgga
      181 tccggactac ttgaaggacc gcaaagtgtt tgtgaccctc accgtcgcct ttcgctatgg
      241 ccgcgaagac ctggacgtcc tgggcttgtc cttccgcaaa gacctgttca tcgccaacta
      301 ccaggccttc cctccaacac ctaacccacc tagaccacct acgcgtctgc aggagcggct
      361 gctgaggaag ctgggccagc atgcccaccc cttttttttc acaatacccc agaacctgcc
      421 cagctccgtc accctgcagc caggcccaga ggatacgggg aaggccctcg gggtcgactt
      481 tgagattcga gccttcgtcg ccaaatcact agaagagaaa agccacaaac ggaactccgt
      541 acgtctggtg atccgaaagg tgcagtttgc cccagagaaa ccagggcccc agccttcagc
      601 tgaaaccaca cgccacttcc tcatgtctga ccggtccctg caccttgagg cttcactgga
      661 caaggagctg tactaccacg gggagcccct caatgtcaat gtccacgtca ccaataactc
      721 caccaagact gtcaagaaga tcaaagtctc tgtgagacag tacgccgaca tcgtcctctt
      781 cagcaccgcc cagtacaagg tccccgtggc tcaggtcgaa caagatgacc aggtgtcgcc
      841 cagttccacg ttctctaagg tgtacaccat aaccccgctg ctcagcaaca accgggagaa
      901 gcgcggcctc gctctagatg ggaagctcaa gcacgaggac accaacctgg cctcgagcac
      961 cattgtgaag gagggtgcca acaaggaggt gctggggata ctggtgtcct acagggtcaa
     1021 ggtgaagctg gtggtgtcac gaggcgggga tgtctcagtg gagctgccat ttgttctcat
     1081 gcaccccaag ccccatgatc acatcgccct tcccaggccc cagtcagctg ttcctgaaac
     1141 agatgccccc gtggacacca acctcattga atttgaaacc aactatgcca cagacgacga
     1201 catcgtgttc gaagactttg cccggctgcg gctcaagggg ctgaaggacg aggactatga
     1261 cgaccagttc tcttagggct ggaagcttgg gtctccctat agtgagtcgt attaatttcg
     1321 ataagccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     1381 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     1441 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     1501 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     1561 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1621 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1681 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1741 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1801 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1861 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1921 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1981 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     2041 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     2101 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     2161 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     2221 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     2281 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     2341 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     2401 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     2461 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     2521 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     2581 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     2641 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     2701 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     2761 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     2821 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     2881 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     2941 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     3001 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     3061 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     3121 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     3181 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     3241 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     3301 cgaaaagtgc cacctgtaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     3361 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     3421 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     3481 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     3541 gcagattgta ctgagagtgc accattcgac gctctccctt atgcgactcc tgcattagga
     3601 agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca
     3661 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac
     3721 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat
     3781 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga
     3841 ggatctggct agcgatgacc ctgctgattg gttcgctgac catttccggg tgcgggacgg
     3901 cgttaccaga aactcagaag gttcgtccaa ccaaaccgac tctgacggca gtttacgaga
     3961 gagatgatag ggtctgcttc agtaagccag atgctacaca attaggcttg tacatattgt
     4021 cgttagaacg cggctacaat taatacataa ccttatgtat catacacata cgattt
//
