LOCUS       sequence-473972-       10016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to express any CDS in T. cruzi in a tetracycline regulatable 
            manner. Modified from Taylor et al. (2006) for easy cloning using 
            Gateway recombination cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10016)
  AUTHORS   Alonso VL, Ritagliati C, Cribb P, Serra EC
  TITLE     Construction of three new Gateway(R) expression plasmids for 
            Trypanosoma cruzi.
  JOURNAL   Mem Inst Oswaldo Cruz. 2014 Dec;109(8):1081-5. doi: 
            10.1590/0074-0276140238. Epub 2014 Nov 21.
  PUBMED    25424446
REFERENCE   2  (bases 1 to 10016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1590/0074-0276140238"; journalName: "Mem Inst Oswaldo Cruz"; 
            date: "2014-12"; volume: "109"; issue: "8"; pages: "1081-5"
COMMENT     Sequence Label: pTcINDEX-GW
FEATURES             Location/Qualifiers
     source          1..10016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..48
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    55..73
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    337..461
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(486..491,492..509,510..516)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             570..1229
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(636..655)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1571..1876
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1755..1774
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1917..2041)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2573..2620
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2705..2752
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(2799..2815)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2799..2815)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2812..2834)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2823..2839
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2847..2853,2854..2871,2872..2877))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2892..2913
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3030..3047)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3201..3789)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3281..3300)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3960..4751,4752..4820))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4583..4602
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4821..4925)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4993..5011
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5049..5071)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5171..5190
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5384..5406
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5398..5415
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5399..5415
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(8291..9316)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8291..9316)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9788..9806)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(9839..9886)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      complement(join(9971..10016,1..2))
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 agttattgct cagcgggcta gcacctgatt aatacgactc actataggga gatctcccta
       61 tcagtgatag agatctagcc cctcgagatc ccccaacgag tttcttcaaa atatgcagcg
      121 gattcactaa gaaacatttt cacgcacgaa agcgaaatta ttatgattgt tattataata
      181 ctttttcttt gttgttttat ccacttatta ttgttgtgtt aaattgtttt tacctttttt
      241 cttttccaac ttcttttatg atgtcttttc tttttttttt tggctctata agttgtcttg
      301 cggccgccgg catcgatacg cgtggatccg tcgatcacaa gtttgtacaa aaaagctgaa
      361 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag
      421 actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc
      481 caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt
      541 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
      601 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
      661 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
      721 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
      781 cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
      841 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
      901 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
      961 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
     1021 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
     1081 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
     1141 ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
     1201 actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc cggcttacta aaagccagat
     1261 aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata
     1321 cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag
     1381 cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca
     1441 accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa
     1501 gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg
     1561 ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt
     1621 tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc
     1681 cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg
     1741 ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg
     1801 ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat
     1861 gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata
     1921 gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat
     1981 ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg
     2041 tgatcgaggc aggcccaagc attgtgcaca acaaatgctt ttagtaatct ctgcataaag
     2101 gtgaatagtt ttcctatcac cggaattatt tttcttccgt gggcttaccg ttttctgtcc
     2161 ttgtaaggtt tctgagatcg cgttcccata tatatatatc tttttttcaa gtgttgattt
     2221 actgaaggag cttttaataa taatttcaag ccagtcataa tcggaatctg aacattcaag
     2281 agattttttt tttcaaacct tcctccatgt ttgcgtgagc gagctagtcg ataggagttt
     2341 gcagctttga gctttgatgg cttgttggtg gtcgttgact ttttttttgt gtgtgatgct
     2401 caccctcacg gcggtttgct ctttgtccat ttcttgtttt ttcttcttct tttttcacat
     2461 aacaaacaaa ccagggagaa aaactttttt aggttttttg tttatatcgc gttttttttt
     2521 gtttctaagg atgtctgacg atcgaatcag gtgctagccc gctgagcaat aactagcata
     2581 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag aactatatcc
     2641 ggatagggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     2701 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
     2761 aggaactata tccggggatc aagcttggcg taatcatggt catagctgtt tcctgtgtga
     2821 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     2881 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     2941 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     3001 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     3061 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     3121 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     3181 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3241 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3301 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3361 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3421 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3481 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3541 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3601 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3661 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3721 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3781 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3841 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3901 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3961 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     4021 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     4081 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     4141 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     4201 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     4261 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     4321 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     4381 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     4441 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     4501 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4561 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4621 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4681 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4741 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4801 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4861 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4921 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     4981 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     5041 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     5101 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     5161 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     5221 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
     5281 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     5341 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     5401 aaaacgacgg ccagtgaatt cgagctcatt tgtgtctagt acatcatgga gcggtattct
     5461 ctccggtggc ccaggtggcg tcggaaggaa ttggtgtccc tcaggatgtt tggtttttcg
     5521 tttttttttt tgcggtcttt tgtttcgcat cgccgtatcg tgtgcggcgc gaccttcttt
     5581 ttggctcatt ggtcagtgag gtttataata aagtggatca cttgggtttt tggccctttg
     5641 cggcgcaccc gcggaatggg ggagggattt tcataatgta gtgtacattg tgtgtgggtc
     5701 gcaaaatgtc atgccgtgtg caatgtttat ttgcgtgctg tgcgtgtcct tttgtgattc
     5761 ccaccacttg tgtgtatttt accttatcgt ctctccctgc cgttggctgt gcgtgaattg
     5821 tgcttcgcat tcgtgggaaa tcaaaagggg aatcaacgtg ttcttttttt tcagaagggg
     5881 agtcaaagcg ccgtgtggat gccaagtctg tgcaaaagtt aactattatt actactgctg
     5941 ctgctgctgc cactcctaca tatgcgcaca agtacactat atatatgcga atacacctgt
     6001 gtgtattatg ctatgtgtgt gtctgtgtgg gtgtgtaggt gatggggtag tggttgtatt
     6061 atagtatagt atatatagta gtatacggca aaaggcaaag acacaagcac ggcgagtacg
     6121 acgaacaaac ccatttcaca catgagtgtg cgccacacac gcgactgctc cttttttttc
     6181 tgccttgcca ccaccccata tgtatattat atatatatat atataagggt gtgtgtgtgg
     6241 tgagagaaaa taggaaatga ttgcgtgtgt gtcgccgcca cgaacctgtc agatgctttc
     6301 ttacggtatt ttgttgcatg cacccgcgta cacacaatat acacacacaa aaaaaacacc
     6361 gatatgatgg cgagtgtgcg attctccttt tattgtcttt ttcctccttt tctgtgctct
     6421 ctgctgtgtg ccaataaaac acactcctcc tatgtttggt gtagtgtgtg ttatttattg
     6481 tccgcgcaaa aaaaaaagcg agtagagaaa cgggggaaaa agtagtaaat aaatggagga
     6541 ctgcttcgca gctgccctgc cgcactgttt ttgctgctgg gttcgcaacg cgatgctgtt
     6601 gagagtgaaa tcacatcaca aaaagcaccc ctcagactgg tgtgaagtgt gaagtatgtg
     6661 tacaaatgcg tgtgtgtgtg tatacacatt gacgaatagc ataccaggga aaaaagggag
     6721 gacactctct ttcgtgcgtg ctgaactctg tgtgtgtgtg ctgccgtttt gtttgcagct
     6781 tgaaatgctt ggttgaatag aaatcggtac ttgattgttg ccgcatttcg aggaatttca
     6841 tccaaaaaaa aaaagaggtc aacaacaacc aactgacaaa ctttgtgttg gacctgtgta
     6901 tgtggtggtg tggtggggga agagaaacac acacacagac aaacacaaac accatgtcat
     6961 tttggcatgc gtgtgcgtgc gtgcgtgcgt tgaatatcct ccacacacaa atatatatat
     7021 ataataatac cacaacaaca tttcaaagga tcatcaatgt gtgtgagacg tgtgcgtttg
     7081 tagcgtgtgt gtatatgtgt tttgtgtgtt gtgtggtgta atgtgtgtgt gtgtcgttcg
     7141 tgcatataca ccttatacac acacacatat aatacataca taaatacaca cacacggcga
     7201 acgccgcgga ccaacaactt gagatctggt tgattctgcc agtagtcata tgcttgtttc
     7261 aaggacttag ccatgcacgc ctcagactag tagctcggtg caccctggga tatttgcgca
     7321 cccacctttc ccgtcgcaat cggcgccgtc cgaacgcgga aatattccga gaataatacg
     7381 gctgggctgc gccgcgcgtt ccccgcgtga aacaattatt gtgcgatgtg cagcatccta
     7441 ttgtggtgct ttaccgcaca ccagtttcct tgcggggctt atttggtgtg tcggattttt
     7501 aattttttga tgattcggag gaatttttta caatttttgg gtacccctaa aaaacttttt
     7561 ggagtttccg gggttcgatc caacttgtgc ttttttaacc gtccatttgt gtgagctcca
     7621 ccgcggtggc ggccgatccc ccgggctgca ggaattcgat ccacacggct agcatactct
     7681 agcggcatat acacaggtgg gctataatgc ccagtattgt tcgaaaaagt ccaaagaaca
     7741 acaacactac gacgcataat gcacaaacac taaactacac cttaaacacc atcagtgaag
     7801 aaaaagaaaa aaaaagaaaa acagcttaaa tccttaaaaa aaaaaaatca cactaaatta
     7861 aaacagtcag ttataaacaa aaagaaactg aacaaccaaa atcttccaat aacgcaaagt
     7921 tgtaaaaaat taccacaagc tgcatcatac acccaatcaa atcatctaca ttttccttgc
     7981 aataccccaa atcctcgtac attgattaaa cattatttca ctcatccttc cgcatcatta
     8041 aattgctgct ttttgtttcc caaagatgac cataaatata atttcaaaaa aaaaaaagaa
     8101 aaaaaagtta tatatttttt tgttgttgtc cttaaaggga aaagtaaaat tgttttgctc
     8161 ttgacactgt gtcacttgga tcgtggcagc accggtaacg atcatcacgg gacacgcatc
     8221 gtgtattata agatccctat tcgcaaaaga aagacctgaa gtcatcgcca cgcctacaac
     8281 cgcatctact ctattccttt gccctcggac gagtgctggg gcgtcggttt ccactatcgg
     8341 cgagtacttc tacacagcca tcggtccaga cggccgcgct tctgcgggcg atttgtgtac
     8401 gcccgacagt cccggctccg gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg
     8461 catcatcgaa attgccgtca accaagctct gatagagttg gtcaagacca atgcggagca
     8521 tatacgcccg gagccgcggc gatcctgcaa gctccggatg cctccgctcg aagtagcgcg
     8581 tctgctgctc catacaagcc aaccacggcc tccagaagaa gatgttggcg acctcgtatt
     8641 gggaatcccc gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg ccattgtccg
     8701 tcaggacatt gttggagccg aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct
     8761 cggcccaaag catcagctca tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca
     8821 tcacagtttg ccagtgatac acatggggat cagcaatcgc gcatatgaaa tcacgccatg
     8881 tagtgtattg accgattcct tgcggtccga atgggccgaa cccgctcgtc tggctaagat
     8941 cggccgcagc gatcgcatcc atggcctccg cgaccggctg cagaacagcg ggcagttcgg
     9001 tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc
     9061 tctcgctaaa ttccccaatg tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt
     9121 gccgataaac ataacgatct ttgtagaaac catcggcgca gctatttacc cgcaggacat
     9181 atccacgccc tcctacatcg aagctgaaag cacgagattc ttcgccctcc gagagctgca
     9241 tcaggtcgga gacgctgtcg aacttttcga tcagaaactt ctcgacagac gtcgcggtga
     9301 gttcaggctt tttcatatct cattgccccc cgggatcaga tctgcagggc ttcccaacct
     9361 taccagaggg cgccccagct ggcaagctag ttctagaatt ctgttcaatg taattgttgt
     9421 tggtagatgg ctttcaaagt aaaaatatca ctttctgcat tcgtgcagga aacgtgggaa
     9481 aagagagaca agtggaatga aggggaggtt gacaattgtg ggttttgtgt ttcttttctt
     9541 gcattcattt acggccgctg taattattat tattattatt attatttgta gaggacagac
     9601 ataaaataaa tttgaggtat tttccgcatt ttgtttttgt ttgtttgttt gcttctgaat
     9661 gcaatgagaa tttgtttgtg ctcttgtcta ataagactgt tgtgtctgtt tttttgagtc
     9721 tcctgaagaa actggcaact gttgaacttc gctcacttcc tgaatgcgga tacgatagat
     9781 gaaatgccct atagtgagtc gtattacaag gtaccccgga tatagttcct cctttcagca
     9841 aaaaacccct caagacccgt ttagaggccc caaggggtta tgctagttat tgctcagcgg
     9901 tggcagcagc caactcagct tcctttcggg ctttgttagc agccctatcc ggatatagtt
     9961 ctcctttcag caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgct
//
