LOCUS       sequence-473982-        6732 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term HALO targeting construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6732)
  TITLE     Hendrich lab plasmids batch1
REFERENCE   2  (bases 1 to 6732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSox2_Halo_Ty2_BactBsd
FEATURES             Location/Qualifiers
     source          1..6732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    4111..4142
                     /label=rox
                     /bound_moiety="Dre recombinase"
     CDS             4683..5081
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    5204..5338
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5241..5260)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5295..5314
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    5354..5385
                     /label=rox
                     /bound_moiety="Dre recombinase"
     primer_bind     complement(6091..6110)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6092..6110)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6117..6134)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6117..6133)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6126..6148)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(6274..6729)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6411..6432)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6623..6642
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagctcga aattaaccct cactaaaggg aacaaaagct ggtaccgggc cccccctcga
     2221 ggtcgaagct tgacccagaa gacagcccgg accgcgtcaa gaggcccatg aacgccttca
     2281 tggtatggtc ccgggggcag cggcgtaaga tggcccagga gaaccccaag atgcacaact
     2341 cggagatcag caagcgcctg ggcgcggagt ggaaactttt gtccgagacc gagaagcggc
     2401 cgttcatcga cgaggccaag cggctgcgcg ctctgcacat gaaggagcac ccggattata
     2461 aataccggcc gcggcggaaa accaagacgc tcatgaagaa ggataagtac acgcttcccg
     2521 gaggcttgct ggcccccggc gggaacagca tggcgagcgg ggttggggtg ggcgccggcc
     2581 tgggtgcggg cgtgaaccag cgcatggaca gctacgcgca catgaacggc tggagcaacg
     2641 gcagctacag catgatgcag gagcagctgg gctacccgca gcacccgggc ctcaacgctc
     2701 acggcgcggc acagatgcaa ccgatgcacc gctacgacgt cagcgccctg cagtacaact
     2761 ccatgaccag ctcgcagacc tacatgaacg gctcgcccac ctacagcatg tcctactcgc
     2821 agcagggcac ccccggtatg gcgctgggct ccatgggctc tgtggtcaag tccgaggcca
     2881 gctccagccc ccccgtggtt acctcttcct cccactccag ggcgccctgc caggccgggg
     2941 acctccggga catgatcagc atgtacctcc ccggcgccga ggtgccggag cccgctgcgc
     3001 ccagtagact gcacatggcc cagcactacc agagcggccc ggtgcccggc acggccatta
     3061 acggcacact gcccctgtcg cacatgggct ctggtggcgg tggctctggc ggaggtgggt
     3121 ccggtggcgg cggaagcgcc atggcagaaa tcggtactgg ctttccattc gacccccatt
     3181 atgtggaagt cctgggcgag cgcatgcact acgtcgatgt tggtccgcgc gatggcaccc
     3241 ctgtgctgtt cctgcacggt aacccgacct cctcctacgt gtggcgcaac atcatcccgc
     3301 atgttgcacc gacccatcgc tgcattgctc cagacctgat cggtatgggc aaatccgaca
     3361 aaccagacct gggttatttc ttcgacgacc acgtccgctt catggatgcc ttcatcgaag
     3421 ccctgggtct ggaagaggtc gtcctggtca ttcacgactg gggctccgct ctgggtttcc
     3481 actgggccaa gcgcaatcca gagcgcgtca aaggtattgc atttatggag ttcatccgcc
     3541 ctatcccgac ctgggacgaa tggccagaat ttgcccgcga gaccttccag gccttccgca
     3601 ccaccgacgt cggccgcaag ctgatcatcg atcagaacgt ttttatcgag ggtacgctgc
     3661 cgatgggtgt cgtccgcccg ctgactgaag tcgagatgga ccattaccgc gagccgttcc
     3721 tgaatcctgt tgaccgcgag ccactgtggc gcttcccaaa cgagctgcca atcgccggtg
     3781 agccagcgaa catcgtcgcg ctggtcgaag aatacatgga ctggctgcac cagtcccctg
     3841 tcccgaagct gctgttctgg ggcaccccag gcgttctgat cccaccggcc gaagccgctc
     3901 gcctggccaa aagcctgcct aactgcaagg ctgtggacat cggcccgggt ctgaatctgc
     3961 tgcaagaaga caacccggac ctgatcggca gcgagatcgc gcgctggctg tcgacgctcg
     4021 aggctagcga agtgcatacc aatcaggacc cgctggatga agtccacaca aaccaagatc
     4081 cactcgatgt cggttaccga taaacgatcg taactttaaa taatgccaat tatttaaagt
     4141 tacatatggc cttctgcagg agcgtacaga acccagggcc ctggcacccg tgcagaccct
     4201 ggcccacccc acctgggcgc tcagtgccca agagatgtcc acacctagga tgtcccgcgg
     4261 tgggtggggg gcccgagaga cgggcaggcc gggggcaggc ctggccatgc ggggccgaac
     4321 cgggcactgc ccagcgtggg gcgcgggggc cacggcgcgc gcccccagcc cccgggccca
     4381 gcaccccaag gcggccaacg ccaaaactct ccctcctcct cttcctcaat ctcgctctcg
     4441 ctcttttttt ttttcgcaaa aggaggggag agggggtaaa aaaatgctgc actgtgcggc
     4501 gaagccggtg agtgagcggc gcggggccaa tcagcgtgcg ccgttccgaa agttgccttt
     4561 tatggctcga gcggccgcgg cggcgcccta taaaacccag cggcgcgacg cgccaccacc
     4621 gccgagaccg cgtccgcccc gcgagcacag agcctcgcct ttgccgatcc atcgatggat
     4681 ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa
     4741 tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg
     4801 gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgc gcagaactcg
     4861 tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg
     4921 gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtt cttctcgatc
     4981 tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga
     5041 ttcgtgaatt gctgccctct ggttatgtgt gggagggcta agctctagat aagtaatgat
     5101 cataatcagc catatcacat ctgtagaggt tttacttgct ttaaaaaacc tcccacacct
     5161 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     5221 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5281 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcca
     5341 tttactgatc acttaacttt aaataatgcc aattatttaa agttaggcgc tgagggctgg
     5401 actgcgaact ggagaagggg agagattttc aaagagatac aagggaattg ggaggggtgc
     5461 aaaaagagga gagtaggaaa aatctgataa tgctcaaaag gaaaaaaaat ctccgcagcg
     5521 aaacgacagc tgcggaaaaa aaccaccaat cccatccaaa ttaacgcaaa aaccgtgatg
     5581 ccgactagaa aacttttatg agagatcttg ggacttcttt ttgggggact atttttgtac
     5641 agagaaaacc tgagggcggc ggggagggcg ggggaatcgg accatgtata gatctggagg
     5701 aaaaaaacta cgcaaaactt ttttttaaag ttctagtggt acgttaggcg cttcgcaggg
     5761 agttcgcaaa agtctttacc agtaatattt agagctagac tccgggcgat gaaaaaaaag
     5821 ttttaatatt tgcaagcaac ttttgtacag tatttatcga gataaacatg gcaatcaaat
     5881 gtccattgtt tataagctga gaatttgcca atatttttcg aggaaagggt tcttgctggg
     5941 ttttgattct gcagcttaaa tttaggaccg ttacaaacaa ggaaggagtt tattcggatt
     6001 tgaacatttt agttttaaaa ttgtacaaaa ggaaaacatg agagcaagta ctggcaagac
     6061 cgttttcgtg gtccgccgat ctccaattcg ccctatagtg agtcgtatta caattcactg
     6121 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     6181 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     6241 tcccaacagt tgcgcagcct gaatggcgaa tggaaattgt aagcgttaat attttgttaa
     6301 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     6361 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     6421 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     6481 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     6541 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     6601 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     6661 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     6721 agggcgcgtc ag
//
