LOCUS       sequence-474038-        8945 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression construct for OsTir1, Bsd resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8945)
  TITLE     Hendrich lab plasmids batch1
REFERENCE   2  (bases 1 to 8945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PB CAG Tir1 Bsd
FEATURES             Location/Qualifiers
     source          1..8945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(71..90)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     281..302
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        465..569
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(570..638,639..1430)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(788..807)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1601..2189
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2090..2109
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2343..2360
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2477..2498
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2513..2518,2519..2536,2537..2543)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2551..2567
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2575..2591
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   2649..2683
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    2958..2991
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        3001..3380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3382..3657
                     /label=chicken beta-actin promoter
     intron          3658..4674
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4598..4620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4682..4701
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    4738..4762
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4770..4779
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4791..4811
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4818..6542
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="TYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVG
                     NCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEEL
                     RMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVE
                     DRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLA
                     KILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPL
                     CAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRV
                     FPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLC
                     ILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIA
                     FAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGC
                     QVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     protein_bind    complement(6543..6567)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(6719..7293,7294..7296,7297..7305)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6886..6903)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7113..7132
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(7792..7811)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7857..7912
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7911..7930)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8273..8289)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8297..8313
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8321..8327,8328..8345,8346..8351))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8366..8387
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    8460..8493
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   8664..8726
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8745..8764)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8746..8764)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8771..8788)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8771..8787)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8780..8802)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8929..8945,1..439)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
       61 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      121 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
      181 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
      241 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
      301 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
      361 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
      421 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      481 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      541 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      601 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      661 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
      721 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
      781 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
      841 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
      901 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
      961 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     1021 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     1081 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     1141 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     1201 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     1261 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     1321 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     1381 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     1441 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1501 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1561 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1621 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1681 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1741 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1801 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1861 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1921 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1981 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2041 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2101 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2161 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2221 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2281 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     2341 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     2401 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     2461 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     2521 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     2581 acagctatga ccatgattac gccaagctcg aaattaaccc tcactatagg gcgaattggg
     2641 taccttaacc ctagaaagat agtctgcgta aaattgacgc atgcattctt gaaatattgc
     2701 tctctctttc taaatagcgc gaatccgtcg ctgtgcattt aggacatctc agtcgccgct
     2761 tggagctccc gtgaggcgtg cttgtcaatg cggtaagtgt cactgatttt gaactataac
     2821 gaccgcgtga gtcaaaatga cgcatgatta tcttttacgt gacttttaag atttaactca
     2881 tacgataatt atattgttat ttcatgttct acttacgtga taacttatta tatatatatt
     2941 ttcttgttat agatatcata acttcgtata atgtatgcta tacgaagtta tgcggccctc
     3001 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     3061 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     3121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     3181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     3241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     3301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     3361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
     3421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
     3481 tgggggcggg gggggggggg gsgcgcgcca ggcggggcgg ggcggggcga ggggcggggc
     3541 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
     3601 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
     3661 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
     3721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
     3781 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
     3841 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
     3901 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
     3961 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     4021 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     4081 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     4141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     4201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     4261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     4321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     4381 ggactttctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     4441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     4501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     4561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     4621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     4681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcct cgaatcaaca
     4741 agtttgtaca aaaaagcagg ctacccgccg ccaccatggg ccccgccgcc cccaagaaga
     4801 agagaaaggt gctggacacg tacttccctg aagaagttgt ggaacatatc ttttcttttc
     4861 ttcctgccca aagagaccgg aatacagtct ctttggtatg taaggtttgg tatgagattg
     4921 aaaggttgtc aaggagagga gtgtttgtgg gaaactgcta tgcggtaagg gctggaagag
     4981 tcgctgccag attccccaat gtgagggcac ttacagttaa gggtaaacca cattttgctg
     5041 atttcaattt ggttcctccg gactggggag gttatgctgg accatggatt gaggctgctg
     5101 ccagaggttg tcatgggttg gaggagttga ggatgaagag gatggttgtg tctgatgagt
     5161 cccttgaact tctggcccgg tccttcccca gattcagagc tttggtcttg atttcttgtg
     5221 agggtttctc tactgacgga ctcgctgcag tcgcttccca ctgtaagttg ttgagggagt
     5281 tggacctcca agaaaatgag gttgaggaca gaggaccaag atggttgtca tgtttccctg
     5341 actcatgtac ttcccttgtt tccttgaatt tcgcgtgtat caaaggagag gtcaatgccg
     5401 ggagtcttga gagattggtc tcaagaagtc ccaacttgcg atcactgagg ctcaacagat
     5461 ctgtgagtgt ggataccttg gcaaagattc tcttgagaac ccccaatttg gaggaccttg
     5521 gaactgggaa tctcacggat gacttccaaa ctgagtctta ctttaagttg actagtgctt
     5581 tggaaaagtg taagatgttg agatcactgt ccggattctg ggatgcttca cctgtttgtc
     5641 tttccttcat ctaccctctt tgtgcccagc tgactggact caacttgtcc tatgctccaa
     5701 cactggatgc gagtgacttg accaagatga tttcaagatg cgtgaagctt caaagacttt
     5761 gggtcctcga ttgtatctcc gacaaaggac ttcaagtggt ggcatcatct tgtaaggatc
     5821 tccaagagtt gagagtgttt ccaagtgact tctatgtggc aggttacagt gctgtaactg
     5881 aggaagggtt ggttgctgtc tcccttggat gtcccaagtt gaattcactc ttgtactttt
     5941 gtcaccaaat gactaacgct gcccttgtga cagtggccaa aaattgccct aacttcacac
     6001 gcttcagact ttgtatcctt gaacctggga aacctgacgt ggtcaccagt cagcccttgg
     6061 acgaaggatt tggggcgatt gtgagagaat gtaagggact ccaaagactc tctatcagtg
     6121 gactcctgac tgacaaggtg tttatgtaca ttggtaagta cgctaagcag ctggaaatgc
     6181 tgtctattgc ctttgccggt gattctgaca aggggatgat gcatgttatg aatggatgta
     6241 agaacttgag aaagcttgag ataagagaca gtccattcgg agatgcagcc ctccttggta
     6301 acttcgcaag atacgaaact atgaggtctc tttggatgtc cagctgtaat gttacactca
     6361 agggatgtca agtgctggcc tcaaaaatgc caatgctgaa tgtggaggtg atcaacgaga
     6421 gagatggatc caatgaaatg gaagagaacc acggagatct tcccaaggtg gaaaagttgt
     6481 atgtctacag aaccactgct ggggcaaggg atgacgctcc aaacttcgtg aaaatactgt
     6541 gaacccagct ttcttgtaca aagtggttga ttcgaggccg ctcgataagc ttgatatcga
     6601 attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaactggct
     6661 cacaaatacc actgagatcg acggtatcga taagcttgat atcgaattcc gccccccccc
     6721 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     6781 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     6841 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     6901 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     6961 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     7021 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     7081 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     7141 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     7201 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     7261 acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatgcc tttgtctcaa
     7321 gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa
     7381 gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat
     7441 gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct
     7501 gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg
     7561 agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata
     7621 gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt
     7681 tatgtgtggg agggttaact agataagtaa tgatcataat cagccatatc acatctgtag
     7741 aggttttact tgctttgtcc gacgcggccc gacgggtccg aggggggtcg atctttttcc
     7801 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
     7861 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     7921 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
     7981 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta
     8041 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
     8101 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
     8161 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
     8221 tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc
     8281 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     8341 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     8401 cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcggc cgcgtcgaga
     8461 taacttcgta taatgtatgc tatacgaagt tatgttttgt tactttatag aagaaatttt
     8521 gagtttttgt ttttttttaa taaataaata aacataaata aattgtttgt tgaatttatt
     8581 attagtatgt aagtgtaaat ataataaaac ttaatatcta ttcaaattaa taaataaacc
     8641 tcgatataca gaccgataaa acacatgcgt caattttacg catgattatc tttaacgtac
     8701 gtcacaatat gattatcttt ctagggttaa agtactccaa ttcgccctat agtgagtcgt
     8761 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     8821 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     8881 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     8941 gcggc
//
