LOCUS       sequence-474040-        8408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting construct to make C-term fusion with GGSG-AID-3xFLAG, 
            roxed PgkPuro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8408)
  TITLE     Hendrich lab plasmids batch2
REFERENCE   2  (bases 1 to 8408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBrg1-GGSG_AID_3xF_PP Target2
FEATURES             Location/Qualifiers
     source          1..8408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2217..2233
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2218..2234
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3382..4065
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="MGSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLNN
                     EPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSG
                     GPEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFMN
                     ERKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAME
                     KCKSRA"
     CDS             4066..4131
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    4179..4203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    4241..4272
                     /label=rox
                     /bound_moiety="Dre recombinase"
     promoter        4322..4821
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4357..4380)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4754..4773
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      4835..4844
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4841..5440
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5340..5360
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5447..6442
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(6464..6481)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6470..6694
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    6742..6773
                     /label=rox
                     /bound_moiety="Dre recombinase"
     primer_bind     complement(7767..7786)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7768..7786)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7793..7810)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7793..7809)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7802..7824)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(7950..8405)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8087..8108)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8299..8318
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagctcga aattaaccct cactaaaggg aacaaaagct ggtaccgggc cccccctcga
     2221 ggtcgacggt atcgataagc ttgatatcgg cataggctcc aacctccacg ggcccatgcc
     2281 cacacacaga cataacgcta cataagtaaa agtaaaagta agccaggtat gatggttcat
     2341 ggacagcttc attcccggca gtcaggaggc aaaggcaggc tctcttaagt tttaggctgg
     2401 cctggtacac atagcaagtt ccaggccagc cagggctata tagagaccct aattttttag
     2461 agggctttct tgtttatttt gcctgcatgt atggatgtgt gccactgtgt gcctggtgat
     2521 cgtggcggct agaagaacgc attggatccc ctggaactgg agttccaggg tgttatgtga
     2581 acacccacta tgtgggttct caggagtgaa ttcgtgtcct ttgtaagagc agccagtttt
     2641 cttagctatt gagtcgtctc tccaacccta aaaatgactc ttaataagat aatggctgca
     2701 ggcatagtag taaactcccg tgatcccagg ccttgggagg tagaagccgg aagatcagga
     2761 ggcgttcatg accatccttg gttgcctagc aagtttgagg ccagcctgag ctctattaga
     2821 ccaaaatggc agtcagaaga catgttcata ctcaagaatg gctataatga gtggctgcct
     2881 cttatggggt tccatgttgg cctgtcccta ccatggtgca gggcaaggtc caagttttga
     2941 tgcagttttc tttttttttt tttcaaagat tcatttattt atttattata tgtaagtata
     3001 ctgtagctgt cttcagacac tccagaagag ggagtcagat ctcgttaagg atggttgtga
     3061 gccactatgt ggttgctggg atttgaaccc aggacctttg gaagagtaat cagtgctctt
     3121 aactgctgag tcgtcttgcc agcctccttg atgcagtttt tttttttttg ccacagtaag
     3181 ataatttatc ccttaaggat ctaggccctt ccacagcatt gagaacacca tggacaaggg
     3241 agagtgccta ggccgcagcc tactcacaaa ctattccccc tgttctttca ggaccgctca
     3301 ggaagtggca gtgaggaaga cggctctggt ggcggtggct ctggcggagg tgggtccggt
     3361 ggcggcggaa gcgccccagg aatgggcagt gtcgagctga atctgaggga gactgagctg
     3421 tgtcttggtc ttcccggtgg agatacagtg gctccggtaa ccggaaacaa gagagggttc
     3481 tcagagacgg ttgatctgaa gctaaatctg aataatgagc ctgcaaacaa ggaaggatct
     3541 acgactcatg acgtcgtgac ttttgattcc aaggagaaga gtgcttgtcc taaagatcca
     3601 gccaaacctc cggccaaggc acaagttgtg ggatggccac cggtgagatc ataccggaag
     3661 aacgtgatgg tttcctgcca aaaatcaagc ggtggcccgg aggcggcggc gttcgtgaag
     3721 gtatcaatgg acggagcacc gtacttgagg aaaatcgatt tgaggatgta taaaagctac
     3781 gatgagcttt ctaatgcttt gtccaacatg ttcagctctt ttaccatggg caaacatgga
     3841 ggagaagaag gaatgataga cttcatgaat gagaggaaat tgatggattt ggtgaatagc
     3901 tgggactatg ttccctctta tgaagacaaa gacggtgatt ggatgctcgt cggcgacgtt
     3961 ccttggccaa tgttcgtcga tacatgcaag cgtttacgtc tcatgaaagg atcggatgcc
     4021 attggtctcg ctccgagggc gatggagaag tgcaagagca gagctgacta caaagaccat
     4081 gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa gtagaagctt
     4141 cgatcggtac caaggcgcat aacgatacca cgatatcaac aagtttgtac aaaaaagcag
     4201 gctggcgccg gaaccaattc agtcgacgtt taaacgatcg taactttaaa taatgccaat
     4261 tatttaaagt tacatatgag aaagtatagg aacttcagct tgatatcgaa ttaattctac
     4321 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
     4381 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
     4441 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
     4501 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
     4561 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
     4621 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
     4681 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
     4741 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
     4801 tcctcatctc cgggcctttc gacctgcagc caacgccacc atggggaccg agtacaagcc
     4861 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc
     4921 gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt
     4981 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     5041 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     5101 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     5161 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     5221 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     5281 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     5341 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     5401 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc ggatccatgc ccacgctact
     5461 gcgggtttat atagacggtc ctcacgggat ggggaaaacc accaccacgc aactgctggt
     5521 ggccctgggt tcgcgcgacg atatcgtcta cgtacccgag ccgatgactt actggcaggt
     5581 gctgggggct tccgagacaa tcgcgaacat ctacaccaca caacaccgcc tcgaccaggg
     5641 tgagatatcg gccggggacg cggcggtggt aatgacaagc gcccagataa caatgggcat
     5701 gccttatgcc gtgaccgacg ccgttctggc tcctcatatc gggggggagg ctgggagctc
     5761 acatgccccg cccccggccc tcaccctcat cttcgaccgc catcccatcg ccgccctcct
     5821 gtgctacccg gccgcgcgat accttatggg cagcatgacc ccccaggccg tgctggcgtt
     5881 cgtggccctc atcccgccga ccttgcccgg cacaaacatc gtgttggggg cccttccgga
     5941 ggacagacac atcgaccgcc tggccaaacg ccagcgcccc ggcgagcggc ttgacctggc
     6001 tatgctggcc gcgattcgcc gcgtttacgg gctgcttgcc aatacggtgc ggtatctgca
     6061 gggcggcggg tcgtggcggg aggattgggg acagctttcg gggacggccg tgccgcccca
     6121 gggtgccgag ccccagagca acgcgggccc acgaccccat atcggggaca cgttatttac
     6181 cctgtttcgg gcccccgagt tgctggcccc caacggcgac ctgtacaacg tgtttgcctg
     6241 ggccttggac gtcttggcca aacgcctccg tcccatgcac gtctttatcc tggattacga
     6301 ccaatcgccc gccggctgcc gggacgccct gctgcaactt acctccggga tggtccagac
     6361 ccacgtcacc acccccggct ccataccgac gatctgcgac ctggcgcgca cgtttgcccg
     6421 ggagatgggg gaggctaact gagctctaga gctcgctgat cagcctcgac tgtgccttct
     6481 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     6541 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     6601 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     6661 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     6721 ggctcgacat actagagtac ttaactttaa ataatgccaa ttatttaaag ttaggcgcgc
     6781 cgatatctag acccagcttc tagttctaga gcagacattc ctgagtcctg accccgaggc
     6841 gctcgtccca gccaagatgg agtagccctt agcagtgatg ggtagcacca gatgtagttt
     6901 cgaacttgga gaactgtaca catgcaatct tccacatttt taggcagaga agtataggcc
     6961 tgtctgtcgg ccctggcctg gcctcgagtc tctaccagca ttaactgtct agagagggga
     7021 cctcctggga gcaccatcca cctccccagg ccccagtcac tgtagctcag tggatgcatg
     7081 cgcgtgccgg ccgctccttg tactgtatct tactggacag ggccagctct ccaggaggct
     7141 cacaggccca gcgggtatgt cagtgtcact ggagtcagac agtaataaat taaagcaatg
     7201 acaagccacc actggctccc tggactcctt gctgtcagca gtggctccgg ggccacagag
     7261 aagaaagaaa gacttttagg aactgggtct aacttatggg caaagtactt gccttgccag
     7321 gtgtatgggt tttgcattcc catcacccac acaccctaaa caagccaagt cagtgagctt
     7381 caagttagag cctccacctc aatgtgtacg tggaaagcaa tcaaagatga tgcctagcat
     7441 ccacctctgg ccctcatgtg cagatgtaca cacactgaat tacatacacg ggacacacac
     7501 atccacacgg aggcagtcca tgacttgcac tggggagatg gtaccatagg cgaaagtgcc
     7561 acaggcacag ggccaggcta atttagtcct gcagtcctgt gctcttaaga tgaaggcaca
     7621 aagaggaacc ccaggcgctc caactagcat gccaggcagt gacaagaccc tgcttcaaat
     7681 gaatcagagc ccacattcag tattgccctc ttacccgatg cgatgcccat gccctcacat
     7741 agccaccgcg gtggagctcc aattcgccct atagtgagtc gtattacaat tcactggccg
     7801 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     7861 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     7921 aacagttgcg cagcctgaat ggcgaatgga aattgtaagc gttaatattt tgttaaaatt
     7981 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     8041 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     8101 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     8161 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     8221 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     8281 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     8341 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     8401 cgcgtcag
//
