LOCUS       sequence-47409-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Chen ZX, Mann JR, Hsieh CL, Riggs AD, Chedin F
  TITLE     Physical and functional interactions between the human DNMT3L 
            protein and members of the de novo methyltransferase family.
  JOURNAL   J Cell Biochem. 2005 Aug 1;95(5):902-17.
  PUBMED    15861382
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biochem."; date: "2005-08-1"; volume: "95(5)"; pages: "902-17"
COMMENT     Sequence Label: pcDNA3/Myc-DNMT3B2
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     26..45
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        26..44
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      58..67
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             67..96
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagcttgcc
       61 accatggagc agaagctgat ctcagaggag gacctggaat tcatgaaggg agacaccagg
      121 catctcaatg gagaggagga cgccggcggg agggaagact cgatcctcgt caacggggcc
      181 tgcagcgacc agtcctccga ctcgccccca atcctggagg ctatccgcac cccggagatc
      241 agaggccgaa gatcaagctc gcgactctcc aagagggagg tgtccagtct gctaagctac
      301 acacaggact tgacaggcga tggcgacggg gaagatgggg atggctctga caccccagtc
      361 atgccaaagc tcttccggga aaccaggact cgttcagaaa gcccagctgt ccgaactcga
      421 aataacaaca gtgtctccag ccgggagagg cacaggcctt ccccacgttc cacccgaggc
      481 cggcagggcc gcaaccatgt ggacgagtcc cccgtggagt tcccggctac caggtccctg
      541 agacggcggg caacagcatc ggcaggaacg ccatggccgt cccctcccag ctcttacctt
      601 accatcgacc tcacagacga cacagaggac acacatggga cgccccagag cagcagtacc
      661 ccctacgccc gcctagccca ggacagccag caggggggca tggagtcccc gcaggtggag
      721 gcagacagtg gagatggaga cagttcagag tatcaggatg ggaaggagt
//
