LOCUS       sequence-474118-        4911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE0354h is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP14129 - pAAV-AiE0354h-minBG-iCre(R297T)-BGH...
FEATURES             Location/Qualifiers
     source          1..4911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      847..856
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             859..879
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1945..2056
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2174..2314
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2174..2303
                     /label=AAV2 ITR
     rep_origin      2389..2844
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2476..2495)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2686..2707
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2861..2880)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2980..3002
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3040..3058)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3126..3230
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3231..3299,3300..4091)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3449..3468)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4262..4850
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4751..4770
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaggtac ctctacattg atggaccttt ctccagaatg
      181 aggagccaaa atcatctgaa ttaagtagag atctcatttg tccctgactc atgataatgt
      241 ctgtgctaca gtcctgcatt aattactttg cccaagtaga aatacactac ccatataaga
      301 atttcacttc tgaacaaaac aaaataaaaa tccacgagtg gtgacctcaa aatgaactgg
      361 ttaaatttaa ggactcattt ttaaaggtca atgtcctttt gctgcaaata cagttcctat
      421 gcagatgaga agttgaacct gacctatgga gccgagcata attagccagc tgcaccctca
      481 taataaggtg cagatggtta attgcaccag caaataactg cttacttgtg ggcacaaaag
      541 cctgtaccta aaatcattga aagtatactt gggactattt atgctcatgg caaacaaaac
      601 acatttccaa ggtttcatct gcgaacagat cacagccagc tccaaatccc tgctttgcgg
      661 gagagccatt cacgctgctg aggtctggtc caagagaggg accactggcc acaaagccac
      721 atatcaactg gtgaaatatt tcttggggtg ctgaatcgtt gttgagagct cgggctgggc
      781 ataaaagtca gggcagagcc atctattgct tacatttgct tctgggatcc agatctttcg
      841 aagctagcca ccatggtgcc caagaagaag aggaaagtct ccaacctgct gactgtgcac
      901 caaaacctgc ctgccctccc tgtggatgcc acctctgatg aagtcaggaa gaacctgatg
      961 gacatgttca gggacaggca ggccttctct gaacacacct ggaagatgct cctgtctgtg
     1021 tgcagatcct gggctgcctg gtgcaagctg aacaacagga aatggttccc tgctgaacct
     1081 gaggatgtga gggactacct cctgtacctg caagccagag gcctggctgt gaagaccatc
     1141 caacagcacc tgggccagct caacatgctg cacaggagat ctggcctgcc tcgcccttct
     1201 gactccaatg ctgtgtccct ggtgatgagg agaatcagaa aggagaatgt ggatgctggg
     1261 gagagagcca agcaggccct ggcctttgaa cgcactgact ttgaccaagt cagatccctg
     1321 atggagaact ctgacagatg ccaggacatc aggaacctgg ccttcctggg cattgcctac
     1381 aacaccctgc tgcgcattgc cgaaattgcc agaatcagag tgaaggacat ctcccgcacc
     1441 gatggtggga gaatgctgat ccacattggc aggaccaaga ccctggtgtc cacagctggt
     1501 gtggagaagg ccctgtccct gggggttacc aagctggtgg agagatggat ctctgtgtct
     1561 ggtgtggctg atgaccccaa caactacctg ttctgccggg tcagaaagaa tggtgtggct
     1621 gccccttctg ccacctccca actgtccacc cgggccctgg aagggatctt tgaggccacc
     1681 caccgcctga tctatggtgc caaggatgac tctgggcaga gatacctggc ctggtctggc
     1741 cactctgcca gagtgggtgc tgccaccgac atggccaggg ctggtgtgtc catccctgaa
     1801 atcatgcagg ctggtggctg gaccaatgtg aacattgtga tgaactacat cagaaacctg
     1861 gactctgaga ctggggccat ggtgaggctg ctcgaagatg gggactgagg cgcgccgaat
     1921 tcaagcttct cgagagatct tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     1981 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     2041 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     2101 ggcaggacag caagggggag gattgggaag acaatagcag gcatgagatc tcacgtgcgg
     2161 accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
     2221 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
     2281 tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt
     2341 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
     2401 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     2461 gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     2521 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     2581 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
     2641 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     2701 aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc
     2761 cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     2821 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
     2881 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     2941 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     3001 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     3061 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     3121 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3181 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     3241 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     3301 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     3361 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     3421 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     3481 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     3541 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     3601 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     3661 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     3721 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     3781 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     3841 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     3901 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     3961 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4021 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4081 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4141 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4201 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4261 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4321 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4381 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     4441 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4501 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4561 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4621 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4681 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4741 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4801 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4861 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
