LOCUS       sequence-474121-        4893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE0028m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP14766 - pAAV-AiE0028m-minBG-iCre(R297T)-BGH...
FEATURES             Location/Qualifiers
     source          1..4893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      829..838
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             841..861
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1927..2038
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2156..2296
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2156..2285
                     /label=AAV2 ITR
     rep_origin      2371..2826
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2458..2477)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2668..2689
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2843..2862)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2962..2984
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3022..3040)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3108..3212
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3213..3281,3282..4073)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3431..3450)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4244..4832
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4733..4752
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgggact ccaggatttt gaaactcact ggtcctgaag
      181 tgatgggaaa ataggcgagg tgtcacagct aatttctggc cctgttttat gagttctgct
      241 cttccttatt ctccctgtca gctcaggaat ccccgaggct gtgcaaactg gctgaacatc
      301 actcaatccg agttttatat ccccttttaa taatgttgag tctattctgt ccaactttga
      361 ttcagacaac ctgcttgtct tgaaaaagtg atatatttaa tcagtggcgc tgtccccagc
      421 ctagctccca ctgcgtgtga ctgcagccgg ggagtggtgc agtgctctgt cactgctgtt
      481 tgaatgggta atgtggagcc catcagctga gaagacagga gctggaactg catgttagga
      541 cacacgatca aaagccactc tccactttct gccaatcaaa cctcatgttg ccttgtgggg
      601 cagacattaa atagattttt ttttccagcc ttatactgtg ggcactttgc cagtttgaca
      661 tgtgaactgt tacaaaaaga tagcagagtt ttgactgctt gctgactgcc taaaaatatt
      721 actgaacact tgtcagaaag aaatgttgag ctcgggctgg gcataaaagt cagggcagag
      781 ccatctattg cttacatttg cttctgggat ccagatcttt cgaagctagc caccatggtg
      841 cccaagaaga agaggaaagt ctccaacctg ctgactgtgc accaaaacct gcctgccctc
      901 cctgtggatg ccacctctga tgaagtcagg aagaacctga tggacatgtt cagggacagg
      961 caggccttct ctgaacacac ctggaagatg ctcctgtctg tgtgcagatc ctgggctgcc
     1021 tggtgcaagc tgaacaacag gaaatggttc cctgctgaac ctgaggatgt gagggactac
     1081 ctcctgtacc tgcaagccag aggcctggct gtgaagacca tccaacagca cctgggccag
     1141 ctcaacatgc tgcacaggag atctggcctg cctcgccctt ctgactccaa tgctgtgtcc
     1201 ctggtgatga ggagaatcag aaaggagaat gtggatgctg gggagagagc caagcaggcc
     1261 ctggcctttg aacgcactga ctttgaccaa gtcagatccc tgatggagaa ctctgacaga
     1321 tgccaggaca tcaggaacct ggccttcctg ggcattgcct acaacaccct gctgcgcatt
     1381 gccgaaattg ccagaatcag agtgaaggac atctcccgca ccgatggtgg gagaatgctg
     1441 atccacattg gcaggaccaa gaccctggtg tccacagctg gtgtggagaa ggccctgtcc
     1501 ctgggggtta ccaagctggt ggagagatgg atctctgtgt ctggtgtggc tgatgacccc
     1561 aacaactacc tgttctgccg ggtcagaaag aatggtgtgg ctgccccttc tgccacctcc
     1621 caactgtcca cccgggccct ggaagggatc tttgaggcca cccaccgcct gatctatggt
     1681 gccaaggatg actctgggca gagatacctg gcctggtctg gccactctgc cagagtgggt
     1741 gctgccaccg acatggccag ggctggtgtg tccatccctg aaatcatgca ggctggtggc
     1801 tggaccaatg tgaacattgt gatgaactac atcagaaacc tggactctga gactggggcc
     1861 atggtgaggc tgctcgaaga tggggactga ggcgcgccga attcaagctt ctcgagagat
     1921 cttcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     1981 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     2041 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     2101 aggattggga agacaatagc aggcatgaga tctcacgtgc ggaccgagcg gccgcaggaa
     2161 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     2221 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     2281 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     2341 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
     2401 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
     2461 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     2521 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     2581 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     2641 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     2701 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
     2761 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     2821 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     2881 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2941 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     3001 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     3061 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3121 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3181 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     3241 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     3301 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     3361 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     3421 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     3481 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     3541 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     3601 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     3661 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     3721 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     3781 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     3841 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     3901 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     3961 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     4021 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     4081 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     4141 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     4201 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4261 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4321 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4381 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4441 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4501 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4561 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4621 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4681 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4741 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4801 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4861 tcctggcctt ttgctggcct tttgctcaca tgt
//
