LOCUS       sequence-474130-       14612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses LRRK2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14612)
  AUTHORS   Wang X, Xu P, Bentley-DeSousa A, Hancock-Cerutti W, Cai S, Johnson 
            BT, Tonelli F, Shao L, Talaia G, Alessi DR, Ferguson SM, De Camilli 
            P
  TITLE     The bridge-like lipid transport protein VPS13C/PARK23 mediates 
            ER-lysosome contacts following lysosome damage.
  JOURNAL   Nat Cell Biol. 2025 May;27(5):776-789. doi: 
            10.1038/s41556-025-01653-6. Epub 2025 Apr 10.
  PUBMED    40211074
REFERENCE   2  (bases 1 to 14612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-025-01653-6"; journalName: "Nat Cell Biol"; date: 
            "2025-05"; volume: "27"; issue: "5"; pages: "776-789"
COMMENT     Sequence Label: GFP-LRRK2
FEATURES             Location/Qualifiers
     source          1..14612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     852..872
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             931..996
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             997..1020
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             join(1024..1026,1027..1737)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1024..1026,1027..1737)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1066..1087)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1327..1346)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1674..1695
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1753..1770
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    9371..9993
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(9570..9589)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(10017..10037)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10035..10332
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             10386..11180
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10440..10459)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11050..11069
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11832..11966
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11869..11888)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11923..11942
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11971..11990)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11972..11990)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12004..12020)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12004..12020)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12017..12039)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12028..12044
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12052..12058,12059..12076,12077..12082))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12097..12118
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12235..12252)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12406..12994)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12486..12505)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13165..13956,13957..14025))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13788..13807
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14026..14130)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14157..14585
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14244..14263)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14454..14475
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attaaccatg gactacaaag accatgacgg tgattataaa
      961 gatcatgaca tcgattacaa ggatgacgat gacaagctgg aagttctgtt ccaggggccc
     1021 cttgtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     1081 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     1141 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     1201 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     1261 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     1321 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     1381 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     1441 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     1501 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     1561 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     1621 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     1681 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggc
     1741 ggtggaggtt cacatcacca tcaccatcac gcggccgcga tggctagtgg cagctgtcag
     1801 gggtgcgaag aggacgagga aactctgaag aagttgatag tcaggctgaa caatgtccag
     1861 gaaggaaaac agatagaaac gctggtccaa atcctggagg atctgctggt gttcacgtac
     1921 tccgagcgcg cctccaagtt atttcaaggc aaaaatatcc atgtgcctct gttgatcgtc
     1981 ttggactcct atatgagagt cgcgagtgtg cagcaggtgg gttggtcact tctgtgcaaa
     2041 ttaatagaag tctgtccagg tacaatgcaa agcttaatgg gaccccagga tgttggaaat
     2101 gattgggaag tccttggtgt tcaccaattg attcttaaaa tgctaacagt tcataatgcc
     2161 agtgtaaact tgtcagtgat tggactgaag accttagatc tcctcctaac ttcaggtaaa
     2221 atcaccttgc tgatactgga tgaagaaagt gatattttca tgttaatttt tgatgccatg
     2281 cactcatttc cagccaatga tgaagtccag aaacttggat gcaaagcttt acatgtgctg
     2341 tttgagagag tctcagagga gcaactgact gaatttgttg agaacaaaga ttatatgata
     2401 ttgttaagtg cgttaacaaa ttttaaagat gaagaggaaa ttgtgcttca tgtgctgcat
     2461 tgtttacatt ccctagcgat tccttgcaat aatgtggaag tcctcatgag tggcaatgtc
     2521 aggtgttata atattgtggt ggaagctatg aaagcattcc ctatgagtga aagaattcaa
     2581 gaagtgagtt gctgtttgct ccataggctt acattaggta attttttcaa tatcctggta
     2641 ttaaacgaag tccatgagtt tgtggtgaaa gctgtgcagc agtacccaga gaatgcagca
     2701 ttgcagatct cagcgctcag ctgtttggcc ctcctcactg agactatttt cttaaatcaa
     2761 gatttagagg aaaagaatga gaatcaagag aatgatgatg agggggaaga agataaattg
     2821 ttttggctgg aagcctgtta caaagcatta acgtggcata gaaagaacaa gcacgtgcag
     2881 gaggccgcat gctgggcact aaataatctc cttatgtacc aaaacagttt acatgagaag
     2941 attggagatg aagatggcca tttcccagct catagggaag tgatgctctc catgctgatg
     3001 cattcttcat caaaggaagt tttccaggca tctgcgaatg cattgtcaac tctcttagaa
     3061 caaaatgtta atttcagaaa aatactgtta tcaaaaggaa tacacctgaa tgttttggag
     3121 ttaatgcaga agcatataca ttctcctgaa gtggctgaaa gtggctgtaa aatgctaaat
     3181 catctttttg aaggaagcaa cacttccctg gatataatgg cagcagtggt ccccaaaata
     3241 ctaacagtta tgaaacgtca tgagacatca ttaccagtgc agctggaggc gcttcgagct
     3301 attttacatt ttatagtgcc tggcatgcca gaagaatcca gggaggatac agaatttcat
     3361 cataagctaa atatggttaa aaaacagtgt ttcaagaatg atattcacaa actggtccta
     3421 gcagctttga acaggttcat tggaaatcct gggattcaga aatgtggatt aaaagtaatt
     3481 tcttctattg tacattttcc tgatgcatta gagatgttat ccctggaagg tgctatggat
     3541 tcagtgcttc acacactgca gatgtatcca gatgaccaag aaattcagtg tctgggttta
     3601 agtcttatag gatacttgat tacaaagaag aatgtgttca taggaactgg acatctgctg
     3661 gcaaaaattc tggtttccag cttataccga tttaaggatg ttgctgaaat acagactaaa
     3721 ggatttcaga caatcttagc aatcctcaaa ttgtcagcat ctttttctaa gctgctggtg
     3781 catcattcat ttgacttagt aatattccat caaatgtctt ccaatatcat ggaacaaaag
     3841 gatcaacagt ttctaaacct ctgttgcaag tgttttgcaa aagtagctat ggatgattac
     3901 ttaaaaaatg tgatgctaga gagagcgtgt gatcagaata acagcatcat ggttgaatgc
     3961 ttgcttctat tgggagcaga tgccaatcaa gcaaaggagg gatcttcttt aatttgtcag
     4021 gtatgtgaga aagagagcag tcccaaattg gtggaactct tactgaatag tggatctcgt
     4081 gaacaagatg tacgaaaagc gttgacgata agcattggga aaggtgacag ccagatcatc
     4141 agcttgctct taaggaggct ggccctggat gtggccaaca atagcatttg ccttggagga
     4201 ttttgtatag gaaaagttga accttcttgg cttggtcctt tatttccaga taagacttct
     4261 aatttaagga aacaaacaaa tatagcatct acactagcaa gaatggtgat cagatatcag
     4321 atgaaaagtg ctgtggaaga aggaacagcc tcaggcagcg atggaaattt ttctgaagat
     4381 gtgctgtcta aatttgatga atggaccttt attcctgact cttctatgga cagtgtgttt
     4441 gctcaaagtg atgacctgga tagtgaagga agtgaaggct catttcttgt gaaaaagaaa
     4501 tctaattcaa ttagtgtagg agaattttac cgagatgccg tattacagcg ttgctcacca
     4561 aatttgcaaa gacattccaa ttccttgggg cccatttttg atcatgaaga tttactgaag
     4621 cgaaaaagaa aaatattatc ttcagatgat tcactcaggt catcaaaact tcaatcccat
     4681 atgaggcatt cagacagcat ttcttctctg gcttctgaga gagaatatat tacatcacta
     4741 gacctttcag caaatgaact aagagatatt gatgccctaa gccagaaatg ctgtataagt
     4801 gttcatttgg agcatcttga aaagctggag cttcaccaga atgcactcac gagctttcca
     4861 caacagctat gtgaaactct gaagagtttg acacatttgg acttgcacag taataaattt
     4921 acatcatttc cttcttattt gttgaaaatg agttgtattg ctaatcttga tgtctctcga
     4981 aatgacattg gaccctcagt ggttttagat cctacagtga aatgtccaac tctgaaacag
     5041 tttaacctgt catataacca gctgtctttt gtacctgaga acctcactga tgtggtagag
     5101 aaactggagc agctcatttt agaaggaaat aaaatatcag ggatatgctc ccccttgaga
     5161 ctgaaggaac tgaagatttt aaaccttagt aagaaccaca tttcatccct atcagagaac
     5221 tttcttgagg cttgtcctaa agtggagagt ttcagtgcca gaatgaattt tcttgctgct
     5281 atgcctttct tgcctccttc tatgacaatc ctaaaattat ctcagaacaa attttcctgt
     5341 attccagaag caattttaaa tcttccacac ttgcggtctt tagatatgag cagcaatgat
     5401 attcagtacc taccaggtcc cgcacactgg aaatctttga acttaaggga actcttattt
     5461 agccataatc agatcagcat cttggacttg agtgaaaaag catatttatg gtctagagta
     5521 gagaaactgc atctttctca caataaactg aaagagattc ctcctgagat tggctgtctt
     5581 gaaaatctga catctctgga tgtcagttac aacttggaac taagatcctt tcccaatgaa
     5641 atggggaaat taagcaaaat atgggatctt cctttggatg aactgcatct taactttgat
     5701 tttaaacata taggatgtaa agccaaagac atcataaggt ttcttcaaca gcgattaaaa
     5761 aaggctgtgc cttataaccg aatgaaactt atgattgtgg gaaatactgg gagtggtaaa
     5821 accaccttat tgcagcaatt aatgaaaacc aagaaatcag atcttggaat gcaaagtgcc
     5881 acagttggca tagatgtgaa agactggcct atccaaataa gagacaaaag aaagagagat
     5941 ctcgtcctaa atgtgtggga ttttgcaggt cgtgaggaat tctatagtac tcatccccat
     6001 tttatgacgc agcgagcatt gtaccttgct gtctatgacc tcagcaaggg acaggctgaa
     6061 gttgatgcca tgaagccttg gctcttcaat ataaaggctc gcgcttcttc ttcccctgtg
     6121 attctcgttg gcacacattt ggatgtttct gatgagaagc aacgcaaagc ctgcatgagt
     6181 aaaatcacca aggaactcct gaataagcga gggttccctg ccatacgaga ttaccacttt
     6241 gtgaatgcca ccgaggaatc tgatgctttg gcaaaacttc ggaaaaccat cataaacgag
     6301 agccttaatt tcaagatccg agatcagctt gttgttggac agctgattcc agactgctat
     6361 gtagaacttg aaaaaatcat tttatcggag cgtaaaaatg tgccaattga atttcccgta
     6421 attgaccgga aacgattatt acaactagtg agagaaaatc agctgcagtt agatgaaaat
     6481 gagcttcctc acgcagttca ctttctaaat gaatcaggag tccttcttca ttttcaagac
     6541 ccagcactgc agttaagtga cttgtacttt gtggaaccca agtggctttg taaaatcatg
     6601 gcacagattt tgacagtgaa agtggaaggt tgtccaaaac accctaaggg aattatttcg
     6661 cgtagagatg tggaaaaatt tctttcaaag aaaaggaaat ttccaaagaa ctacatgaca
     6721 cagtatttta agctcctaga aaaattccag attgctttgc caataggaga agaatatttg
     6781 ctggttccaa gcagtttgtc tgaccacagg cctgtgatag agcttcccca ttgtgagaac
     6841 tctgaaatta tcatccgact atatgaaatg ccttattttc caatgggatt ttggtcaaga
     6901 ttaatcaatc gattacttga gatttcacct tacatgcttt cagggagaga acgagcactt
     6961 cgcccaaaca gaatgtattg gcgacaaggc atttacttaa attggtctcc tgaagcttat
     7021 tgtctggtag gatctgaagt cttagacaat catccagaga gtttcttaaa aattacagtt
     7081 ccttcttgta gaaaaggctg tattcttttg ggccaagttg tggaccacat tgattctctc
     7141 atggaagaat ggtttcctgg gttgctggag attgatattt gtggtgaagg agaaactctg
     7201 ttgaagaaat gggcattata tagttttaat gatggcgaag aacatcaaaa aatcttactt
     7261 gatgacttga tgaagaaagc agaggaagga gatctcttag taaatccaga tcaaccaagg
     7321 ctcaccattc caatatctca gattgcccct gacttgattt tggctgacct gcctagaaat
     7381 attatgttga ataatgatga gttggaattt gaacaagctc cagagtttct cctaggtgat
     7441 ggcagttttg gatcagttta ccgagcagcc tatgaaggag aagaagtggc tgtgaagatt
     7501 tttaataaac atacatcact caggctgtta agacaagagc ttgtggtgct ttgccacctc
     7561 caccacccca gtttgatatc tttgctggca gctgggattc gtccccggat gttggtgatg
     7621 gagttagcct ccaagggttc cttggatcgc ctgcttcagc aggacaaagc cagcctcact
     7681 agaaccctac agcacaggat tgcactccac gtagctgatg gtttgagata cctccactca
     7741 gccatgatta tataccgaga cctgaaaccc cacaatgtgc tgcttttcac actgtatccc
     7801 aatgctgcca tcattgcaaa gattgctgac tacggcattg ctcagtactg ctgtagaatg
     7861 gggataaaaa catcagaggg cacaccaggg tttcgtgcac ctgaagttgc cagaggaaat
     7921 gtcatttata accaacaggc tgatgtttat tcatttggtt tactactcta tgacattttg
     7981 acaactggag gtagaatagt agagggtttg aagtttccaa atgagtttga tgaattagaa
     8041 atacaaggaa aattacctga tccagttaaa gaatatggtt gtgccccatg gcctatggtt
     8101 gaaaaattaa ttaaacagtg tttgaaagaa aatcctcaag aaaggcctac ttctgcccag
     8161 gtctttgaca ttttgaattc agctgaatta gtctgtctga cgagacgcat tttattacct
     8221 aaaaacgtaa ttgttgaatg catggttgct acacatcaca acagcaggaa tgcaagcatt
     8281 tggctgggct gtgggcacac cgacagagga cagctctcat ttcttgactt aaatactgaa
     8341 ggatacactt ctgaggaagt tgctgatagt agaatattgt gcttagcctt ggtgcatctt
     8401 cctgttgaaa aggaaagctg gattgtgtct gggacacagt ctggtactct cctggtcatc
     8461 aataccgaag atgggaaaaa gagacatacc ctagaaaaga tgactgattc tgtcacttgt
     8521 ttgtattgca attccttttc caagcaaagc aaacaaaaaa attttctttt ggttggaacc
     8581 gctgatggca agttagcaat ttttgaagat aagactgtta agcttaaagg agctgctcct
     8641 ttgaagatac taaatatagg aaatgtcagt actccattga tgtgtttgag tgaatccaca
     8701 aattcaacgg aaagaaatgt aatgtgggga ggatgtggca caaagatttt ctccttttct
     8761 aatgatttca ccattcagaa actcattgag acaagaacaa gccaactgtt ttcttatgca
     8821 gctttcagtg attccaacat cataacagtg gtggtagaca ctgctctcta tattgctaag
     8881 caaaatagcc ctgttgtgga agtgtgggat aagaaaactg aaaaactctg tggactaata
     8941 gactgcgtgc actttttaag ggaggtaatg gtaaaagaaa acaaggaatc aaaacacaaa
     9001 atgtcttatt ctgggagagt gaaaaccctc tgccttcaga agaacactgc tctttggata
     9061 ggaactggag gaggccatat tttactcctg gatctttcaa ctcgtcgact tatacgtgta
     9121 atttacaact tttgtaattc ggtcagagtc atgatgacag cacagctagg aagccttaaa
     9181 aatgtcatgc tggtattggg ctacaaccgg aaaaatactg aaggtacaca aaagcagaaa
     9241 gagatacaat cttgcttgac cgtttgggac atcaatcttc cacatgaagt gcaaaattta
     9301 gaaaaacaca ttgaagtgag aaaagaatta gctgaaaaaa tgagacgaac atctgttgag
     9361 taaggatccc gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt
     9421 tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga
     9481 ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt
     9541 tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc
     9601 acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc
     9661 tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg
     9721 gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat
     9781 ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc
     9841 tgtccttctg attttaaaat aactatacca gcaggaggac gtccagacac agcataggct
     9901 acctggccat gcccaaccgg tgggacattt gagttgcttg cttggcactg tcctctcatg
     9961 cgttgggtcc actcagtaga tgcctgttga attgggtacg cggccagctt ggctgtggaa
    10021 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
    10081 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
    10141 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
    10201 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
    10261 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
    10321 aggctttttt ggaggaattg atcagcttgg gatctgatca agagacagga tgaggatcgt
    10381 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
    10441 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
    10501 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
    10561 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
    10621 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
    10681 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
    10741 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
    10801 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
    10861 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
    10921 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
    10981 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
    11041 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
    11101 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
    11161 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
    11221 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
    11281 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
    11341 cttcgcccac cccgggctcg atcccctcgc gagttggttc agctgctgcc tgaggctgga
    11401 cgacctcgcg gagttctacc ggcagtgcaa atccgtcggc atccaggaaa ccagcagcgg
    11461 ctatccgcgc atccatgccc ccgaactgca ggagtgggga ggcacgatgg ccgctttggt
    11521 cgacccggac gggacgctcc tgcgcctgat acagaacgaa ttgcttgcag gcatctcatg
    11581 agtgtgtctt cccgttttcc gcctgaggtc actgcgtgga tggagcgctg gcgcctgctg
    11641 cgcgacggcg agctgctcac cacccactcg ccaagctgga accgtaaaaa ggccgcgttg
    11701 ctggcgtttt tccataggct ccgccgatca taatcagcca taccacattt gtagaggttt
    11761 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
    11821 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    11881 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    11941 tcaatgtatc ttatcatgtc tggatcaatt ccctatagtg agtcgtatta aattcgtaat
    12001 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
    12061 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
    12121 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
    12181 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
    12241 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    12301 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    12361 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    12421 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    12481 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    12541 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    12601 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    12661 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    12721 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    12781 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    12841 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    12901 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    12961 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    13021 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    13081 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    13141 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    13201 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    13261 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    13321 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    13381 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    13441 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    13501 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    13561 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    13621 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    13681 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    13741 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    13801 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    13861 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    13921 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    13981 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    14041 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    14101 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc
    14161 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
    14221 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
    14281 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
    14341 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
    14401 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
    14461 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
    14521 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
    14581 gaattttaac aaaatattaa cgcttacaat tt
//
