LOCUS       sequence-474178-       11527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11527)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 11527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag TYK2
FEATURES             Location/Qualifiers
     source          1..11527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    805..1004
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1205..1621
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1551..1573
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1589..1605
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1639..1648
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1645..1686
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             1711..1734
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1741..1765
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5327..5351)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5410..5871
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5466..5483)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5693..5712
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5876..6670
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5930..5949)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6540..6559
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             6857..7326
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        7362..7466
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7467..7535,7536..8327)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7685..7704)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10093..10681
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10582..10601
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10835..10852
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10956..11383)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11066..11087)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11278..11297
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgcg
      601 ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag
      661 ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac
      721 ccgtcagcgg gggtctttca tttgggggct cgtccgggat cgggagaccc ctgcccaggg
      781 accaccgacc caccaccggg aggcaagctg gccagcaact tatctgtgtc tgtccgattg
      841 tctagtgtct atgactgatt ttatgcgcct gcgtcggtac tagttagcta actagctctg
      901 tatctggcgg acccgtggtg gaactgacga gttctgaaca cccggccgca accctgggag
      961 acgtcccagg gactttgggg gccgtttttg tggcccgacc tgaggaaggg agtcgatgtg
     1021 gaatccgacc ccgtcaggat atgtggttct ggtaggagac gagaacctaa aacagttccc
     1081 gcctccgtct gaatttttgc tttcggtttg gaaccgaagc cgcgcgtctt gtctgctgca
     1141 gcgctgcagc atcgttctgt gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa
     1201 ttagggccag actgttacca ctcccttaag tttgacctta ggtcactgga aagatgtcga
     1261 gcggatcgct cacaaccagt cggtagatgt caagaagaga cgttgggtta ccttctgctc
     1321 tgcagaatgg ccaaccttta acgtcggatg gccgcgagac ggcaccttta accgagacct
     1381 catcacccag gttaagatca aggtcttttc acctggcccg catggacacc cagaccaggt
     1441 cccctacatc gtgacctggg aagccttggc ttttgacccc cctccctggg tcaagccctt
     1501 tgtacaccct aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc
     1561 tcctcgttcg accccgcctc gatcctccct ttatccagcc ctcactcctt ctctaggcgc
     1621 cggccggatc cagaattcgc caccatgggg tcttcaaaat ctaaaccaaa ggaccccagc
     1681 cagcgccggc gcaggatccg aggttacctt gactacaaag acgatgacga caagcaattg
     1741 acaagtttgt acaaaaaagt tggcatgcct ctgcgccact gggggatggc caggggcagt
     1801 aagcccgttg gggatggagc ccagcccatg gctgccatgg gaggcctgaa ggtgcttctg
     1861 cactgggctg gtccaggcgg cggggagccc tgggtcactt tcagtgagtc atcgctgaca
     1921 gctgaggaag tctgcatcca cattgcacat aaagttggta tcactcctcc ttgcttcaat
     1981 ctctttgccc tcttcgatgc tcaggcccaa gtctggttgc ccccaaacca catcctagag
     2041 atccccagag atgcaagcct gatgctatat ttccgcataa ggttttattt ccggaactgg
     2101 catggcatga atcctcggga accggctgtg taccgttgtg ggcccccagg aaccgaggca
     2161 tcctcagatc agacagcaca ggggatgcaa ctcctggacc cagcctcatt tgagtacctc
     2221 tttgagcagg gcaagcatga gtttgtgaat gacgtggcat cactgtggga gctgtcgacc
     2281 gaggaggaga tccaccactt taagaatgag agcctgggca tggcctttct gcacctctgt
     2341 cacctcgctc tccgccatgg catccccctg gaggaggtgg ccaagaagac cagcttcaag
     2401 gactgcatcc cgcgctcctt ccgccggcat atccggcagc acagcgccct gacccggctg
     2461 cgccttcgga acgtcttccg caggttcctg cgggacttcc agccgggccg actctcccag
     2521 cagatggtca tggtcaaata cctagccaca ctcgagcggc tggcaccccg cttcggcaca
     2581 gagcgtgtgc ccgtgtgcca cctgaggctg ctggcccagg ccgaggggga gccctgctac
     2641 atccgggaca gtggggtggc ccctacagac cctggccctg agtctgctgc tgggccccca
     2701 acccacgagg tgctggtgac aggcactggt ggcatccagt ggtggccagt agaggaggag
     2761 gtgaacaagg aggagggttc tagtggcagc agtggcagga acccccaagc cagcctgttt
     2821 gggaagaagg ccaaggctca caaggcagtc ggccagccgg cagacaggcc gcgggagcca
     2881 ctgtgggcct acttctgtga cttccgggac atcacccacg tggtgctgaa agagcactgt
     2941 gtcagcatcc accggcagga caacaagtgc ctggagctga gcttgccttc ccgggctgcg
     3001 gcgctgtcct tcgtgtcgct ggtggacggc tatttccgcc tgacggccga ctccagccac
     3061 tacctgtgcc acgaggtggc tcccccacgg ctggtgatga gcatccggga tgggatccac
     3121 ggacccctgc tggagccatt tgtgcaggcc aagctgcggc ccgaggacgg cctgtacctc
     3181 attcactgga gcaccagcca cccctaccgc ctgatcctca cagtggccca gcgtagccag
     3241 gcaccagacg gcatgcagag cttgcggctc cgaaagttcc ccattgagca gcaggacggg
     3301 gccttcgtgc tggagggctg gggccggtcc ttccccagcg ttcgggaact tggggctgcc
     3361 ttgcagggct gcttgctgag ggccggggat gactgcttct ctctgcgtcg ctgttgcctg
     3421 ccccaaccag gagaaacctc caatctcatc atcatgcggg gggctcgggc cagccccagg
     3481 acactcaacc tcagccagct cagcttccac cgggttgacc agaaggagat cacccagctg
     3541 tcccacttgg gccagggcac aaggaccaac gtgtatgagg gccgcctgcg agtggagggc
     3601 agcggggacc ctgaggaggg caagatggat gacgaggacc ccctcgtgcc tggcagggac
     3661 cgtgggcagg agctacgagt ggtgctcaaa gtgctggacc ctagtcacca tgacatcgcc
     3721 ctggccttct acgagacagc cagcctcatg agccaggtct cccacacgca cctggccttc
     3781 gtgcatggcg tctgtgtgcg cggccctgaa aatatcatgg tgacagagta cgtggagcac
     3841 ggacccctgg atgtgtggct gcggagggag cggggccatg tgcccatggc ttggaagatg
     3901 gtggtggccc agcagctggc cagcgccctc agctacctgg agaacaagaa cctggttcat
     3961 ggtaatgtgt gtggccggaa catcctgctg gcccggctgg ggttggcaga gggcaccagc
     4021 cccttcatca agctgagtga tcctggcgtg ggcctgggcg ccctctccag ggaggagcgg
     4081 gtggagagga tcccctggct ggcccccgaa tgcctaccag gtggggccaa cagcctaagc
     4141 accgccatgg acaagtgggg gtttggcgcc accctcctgg agatctgctt tgacggagag
     4201 gcccctctgc agagccgcag tccctccgag aaggagcatt tctaccagag gcagcaccgg
     4261 ctgcccgagc cctcctgccc acagctggcc acactcacca gccagtgtct gacctatgag
     4321 ccaacccaga ggccatcatt ccgcaccatc ctgcgtgacc tcacccggct gcagccccac
     4381 aatcttgctg acgtcttgac tgtgaacccg gactcaccgg cgtcggaccc tacggttttc
     4441 cacaagcgct atttgaaaaa gatccgagat ctgggcgagg gtcacttcgg caaggtcagc
     4501 ttgtactgct acgatccgac caacgacggc actggcgaga tggtggcggt gaaagccctc
     4561 aaggcagact gcggccccca gcaccgctcg ggctggaagc aggagattga cattctgcgc
     4621 acgctctacc acgagcacat catcaagtac aagggctgct gcgaggacca aggcgagaag
     4681 tcgctgcagc tggtcatgga gtacgtgccc ctgggcagcc tccgagacta cctgccccgg
     4741 cacagcatcg ggctggccca gctgctgctc ttcgcccagc agatctgcga gggcatggcc
     4801 tatctgcact cgcagcacta catccaccga gacctagccg cgcgcaacgt gctgctggac
     4861 aacgacaggc tggtcaagat cggggacttt ggcctagcca aggccgtgcc cgaaggccac
     4921 gagtactacc gcgtgcgcga ggatggggac agccccgtgt tctggtatgc cccagagtgc
     4981 ctgaaggagt ataagttcta ctatgcgtca gatgtctggt ccttcggggt gaccctgtat
     5041 gagctgctga cgcactgtga ctccagccag agccccccca cgaaattcct tgagctcata
     5101 ggcattgctc agggtcagat gacagttctg agactcactg agttgctgga acgaggggag
     5161 aggctgccac ggcccgacaa atgtccctgt gaggtctatc atctcatgaa gaactgctgg
     5221 gagacagagg cgtcctttcg cccaaccttc gagaacctca tacccattct gaagacagtc
     5281 catgagaagt accaaggcca ggccccttca gtgttcagcg tgtgctgccc aactttcttg
     5341 tacaaagtgg tttgatctag tcgaccaatt ccggttattt tccaccatat tgccgtcttt
     5401 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     5461 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     5521 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     5581 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     5641 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     5701 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     5761 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaacgtcta
     5821 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata ataccatgat
     5881 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     5941 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     6001 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     6061 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     6121 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     6181 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     6241 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     6301 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     6361 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     6421 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     6481 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     6541 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     6601 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     6661 gttcttctga gcgggactct ggggttcgca tcgataaaat aaaagatttt atttagtctc
     6721 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg
     6781 ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca
     6841 ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     6901 cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
     6961 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc
     7021 cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga
     7081 ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct
     7141 gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt
     7201 gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg
     7261 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt
     7321 ctttcacagg ccagtggggc cgcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     7381 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     7441 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     7501 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     7561 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     7621 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     7681 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     7741 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     7801 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     7861 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     7921 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     7981 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     8041 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     8101 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     8161 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     8221 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     8281 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     8341 tttactcata tatactttag attgatttgc ggccgcgaaa aggacaagca gcgaaaattc
     8401 acgccccctt gggaggtggc ggcatatgca aaggatagca ctcccactct actactgggt
     8461 atcatatgct gactgtatat gcatgaggat agcatatgct acccggatac agattaggat
     8521 agcatatact acccagatat agattaggat agcatatgct acccagatat agattaggat
     8581 agcctatgct acccagatat aaattaggat agcatatact acccagatat agattaggat
     8641 agcatatgct acccagatat agattaggat agcctatgct acccagatat agattaggat
     8701 agcatatgct atccagatat ttgggtagta tatgctaccc atggcaacat tagcccaccg
     8761 tgctctcagc gacctcgtga atatgaggac caacaaccct gtgcttggcg ctcaggcgca
     8821 agtgtgtgta atttgtcctc cagatcgcag caatcgcgcc cctatcttgg cccgcccacc
     8881 tacttatgca ggtattcccc ggggtgccat tagtggtttt gtgggcaagt ggtttgaccg
     8941 cagtggttag cggggttaca atcagccaag ttattacacc cttattttac agtccaaaac
     9001 cgcagggcgg cgtgtggggg ctgacgcgtg cccccactcc acaatttcaa aaaaaagagt
     9061 ggccacttgt ctttgtttat gggccccatt ggcgtggagc cccgtttaat tttcgggggt
     9121 gttagagaca accagtggag tccgctgctg tcggcgtcca ctctctttcc ccttgttaca
     9181 aatagagtgt aacaacatgg ttcacctgtc ttggtccctg cctgggacac atcttaataa
     9241 ccccagtatc atattgcact aggattatgt gttgcccata gccataaatt cgtgtgagat
     9301 ggacatccag tctttacggc ttgtccccac cccatggatt tctattgtta aagatattca
     9361 gaatgtttca ttcctacact agtatttatt gcccaagggg tttgtgaggg ttatattggt
     9421 gtcatagcac aatgccacca ctgaaccccc cgtccaaatt ttattctggg ggcgtcacct
     9481 gaaaccttgt tttcgagcac ctcacataca ccttactgtt cacaactcag cagttattct
     9541 attagctaaa cgaaggagaa tgaagaagca ggcgaagatt caggagagtt cactgcccgc
     9601 tccttgatct tcagccactg cccttgtgac taaaatggtt cactaccctc gtggaatcct
     9661 gaccccatgt aaataaaacc gtgacagctc atggggtggg agatatcgct gttccttagg
     9721 acccttttac taaccctaat tcgatagcat atgcttcccg ttgggtaaca tatgctattg
     9781 aattagggtt agtctggata gtatatacta ctacccggga agcatatgct acccgtttag
     9841 ggttaacaag ggggccttat aaacactatt gctaatgccc tcttgagggt ccgcttatcg
     9901 gtagctacac aggcccctct gattgacgtt ggtgtagcct cccgtagtct tcctgggccc
     9961 ctgggaggta catgtcggcc gcaaaaggat ctaggtgaag atcctttttg ataatctcat
    10021 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    10081 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    10141 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    10201 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
    10261 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    10321 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    10381 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    10441 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    10501 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    10561 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    10621 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    10681 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    10741 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    10801 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    10861 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcaacta
    10921 tggttggcgc gttgtaaacg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
    10981 cagctcattt tttaaccaat agaccgaaat cggcaaaatc ccttataaat caaaagaata
    11041 gcccgagata gagttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
    11101 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
    11161 atcacccaaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
    11221 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
    11281 gaagaaagcg aaaggagcgg gcgctaaggc gctggcaagt gtagcggtca cgctgcgcgt
    11341 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgccgatct tcaatattgg
    11401 ccattagcca tattattcat tggttatata gcataaatca atattggcta ttggccattg
    11461 catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg
    11521 ccatgtt
//
