LOCUS       sequence-474215-        1901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes tTDH1 as a Type 4b part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK066
FEATURES             Location/Qualifiers
     source          1..1901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(255..289)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(360..1019)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     934..953
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1201..1789
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1690..1709
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcct ggcataaagc aatcttgatg aggataatga tttttttttg aatatacata
       61 aatactaccg tttttctgct agattttgtg atgacgtaaa taagtacata ttacttttta
      121 agccaagaca agattaagca ttaactttac ccttttcttt ctaagtttca atattagtta
      181 tcactgttta aaagttatgg cgagaacgtc ggcggttaaa atatattacc ctgaacggct
      241 gtgagaccag accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
      301 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
      361 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
      421 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
      481 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
      541 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgaaacgaa aaacatattc
      601 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
      661 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
      721 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
      781 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
      841 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
      901 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
      961 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     1021 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     1081 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga ccaaaatccc
     1141 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     1201 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     1261 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     1321 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     1381 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     1441 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     1501 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     1561 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     1621 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     1681 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     1741 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     1801 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctggccttt tgctcacatg
     1861 ttctttcctg cgttatcccc tgattctgtg gataaccgta g
//
