LOCUS       sequence-474217-        8896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0 and a
            membrane-localized EGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8896)
  AUTHORS   Zheng Y, Cai R, Wang K, Zhang J, Zhuo Y, Dong H, Zhang Y, Wang Y, 
            Deng F, Ji E, Cui Y, Fang S, Zhang X, Zhang K, Wang J, Li G, Miao X,
            Wang Z, Yang Y, Li S, Grimm J, Johnsson K, Schreiter E, Lavis L, 
            Chen Z, Mu Y, Li Y
  TITLE     In vivo multiplex imaging of dynamic neurochemical networks with 
            designed far-red dopamine sensors.
  JOURNAL   Science. 2025 Jun 5;388: 6751. doi: 
            https://doi.org/10.1126/science.adt7705.
  PUBMED    39763912
REFERENCE   2  (bases 1 to 8896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2025-06-5"; volume: "388: 6751. doi: https"; pages: 
            "//doi.org/10.1126/science.adt7705"
COMMENT     Sequence Label: pDisplay-GRAB-HaloDA1.0-IRES-EGFP-CAAX
FEATURES             Location/Qualifiers
     source          1..8896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     638..657
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     737..796
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3109..3660
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3253..3270)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3479..3498
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3672..3674,3675..3677,3678..4388)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3672..3674,3675..3677,3678..4388)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3717..3738)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3978..3997)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4325..4346
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4446..4475
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4479..4625
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(4647..4664)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4653..4877
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(5009..5597)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5089..5108)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5926..5973
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5998..6017
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(6205..6999)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(6205..6999)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6316..6335)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6926..6945
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(7098..7117)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        7196..7387
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     7344..7364
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(7447..8238,8239..8307))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8070..8089
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8308..8412)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8439..8894
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8526..8545)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8736..8757
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccctgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg
      721 cttggggata tccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt
      781 tccaggttcc actggtgaca caagtttgta caaaaaagtt ggcaccaccg gtaggactct
      841 gaacacctct gccatggacg ggactgggct ggtggtggag agggacttct ctgttcgtat
      901 cctcactgcc tgtttcctgt cgctgctcat cctgtccacg ctcctgggga acacgctggt
      961 ctgtgctgcc gttatcaggt tccgacacct gcggtccaag gtgaccaact tctttgtcat
     1021 ctccttggct gtgtcagatc tcttggtggc cgtcctggtc atgccctgga aggcagtggc
     1081 tgagattgct ggcttctggc cctttgggtc cttctgtaac atctgggtgg cctttgacat
     1141 catgtgctcc actgcatcca tcctcaacct ctgtgtgatc agcgtggaca ggtattgggc
     1201 tatctccagc cctttccggt atgagagaaa gatgaccccc aaggcagcct tcatcctgat
     1261 cagtgtggca tggaccttgt ctgtactcat ctccttcatc ccagtgcagc tcagctggca
     1321 caaggcaaaa cccacaagcc cctctgatgg aaatgccact tccctggctg agaccataga
     1381 caactgtgac tccagcctca gcaggacata tgccatctca tcctctgtaa taagctttta
     1441 catccctgtg gccatcatga ttgtctgcta caccaggatc tacaggattg ccaagcgtcg
     1501 cacccgcgtg ccacccagcc gccggggtcc ggacgccgtc gccgcgccgc cggggggcac
     1561 cgagcgcagg cccaacggta tgtacttgcc agagatgtgc cgtgagacct tccaggccta
     1621 ccgcaccacc gacgtcggcc gcaagctgat catcaccaag aacgttttta tcgagggtac
     1681 gctgccgatg ggtgtcgtcc gcccgctgac tgaagtcgag atggaccatt accgcgagcc
     1741 gttcctgaat cctgttgacc gcgagccact gtggcgcttc ccaaacgagc tgccaatcgc
     1801 cggtgagcca gcgaacatcg tcgcgctggt cgaagaatac atggactggc tgcaccagtc
     1861 ccctgtcccg aagctgctgt tctggggcac cccaggcgtt ctgatcccac cggccgaagc
     1921 cgctcgcctg gccaaaagcc tgcctaactg caaggctgtg gacatcggcc cgggtctgaa
     1981 tctgctgcaa gaagacaacc cggacctgat cggcagcgag atcgcgcgct ggctgtcgac
     2041 gctcgagatt tccggcggag gaacaggtgg ttctggtgga acagggggta gcggaggtac
     2101 aggaggaagt atggcggaga tcggaactgg attcccgttt gatccgcatt atgtggaagt
     2161 tctgggagag cgcatgcatt atgtggacgt tggtcctcgt gatgggacac cagtgctgtt
     2221 ccttcacggc aatccgacat cgtcgtacgt gtggcgtaat atcatcccgc acgttgcccc
     2281 cacgcaccgc tgcattgccc ctgacttaat tggtatgggg aaaagtgata agcctgatct
     2341 ggggtacttc tttgacgacc acgtacgctt catggatgct tttattgaag cattgggttt
     2401 ggaggaagta gttttggtga tccatgattg gggtagtgct ctggggttcc attgggccaa
     2461 gcgtaaccca gaacgcgtga aaggaattgc ctttatggag ttcatccgtc cgattccaac
     2521 atgggacgac tggcctgagt ttgcaaactg gaagactctg tcggtgatca tgggtgtgtt
     2581 tgtgtgctgt tggctacctt tcttcatctt gaactgcatt cggcccttct gtgggtctgg
     2641 gtggacgcag cccttctgca ttgattccaa cacctttgac gtgtttgtgt ggtttgggtg
     2701 ggctaattca tccttgaacc ccatcattta tgcctttaat gctgattttc ggaaggcatt
     2761 ttcaaccctc ttaggatgct acagactttg ccctgcgacg aataatgcca tagagacggt
     2821 gagtatcaat aacaatgggg ccgcgatgtt ttccagccat catgagccac gaggctccat
     2881 ctccaaggag tgcaatctgg tttacctgat cccacatgct gtgggctcct ctgaggacct
     2941 gaaaaaggag gaggcagctg gcatcgccag acccttggag aagctgtccc cagccctatc
     3001 ggtcatattg gactatgaca ctgacgtctc tctggagaag atccaaccca tcacacaaaa
     3061 cggtcagcac ccaacctgat aagctagcgc ccctctccct cccccctaac gttactggcc
     3121 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     3181 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     3241 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt aaatgtcgtg aaggaagcag
     3301 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     3361 accccccacc tggcgacagg tgcctctgcg gccaaagcca cgtgtataag atacacctgc
     3421 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     3481 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     3541 gggatctgat ctggggcctc ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa
     3601 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     3661 tggccacaac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     3721 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     3781 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     3841 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     3901 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     3961 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     4021 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     4081 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     4141 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     4201 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     4261 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     4321 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     4381 tgtacaagga tggtaaaaag aagaaaaaga agtcaaagac aaagtgtgta attatgtaag
     4441 tcgacgaaca aaaactcatc tcagaagagg atctgaatgc tgtgggccag gacacgcagg
     4501 aggtcatcgt ggtgccacac tccttgccct ttaaggtggt ggtgatctca gccatcctgg
     4561 ccctggtggt gctcaccatc atctccctta tcatcctcat catgctttgg cagaagaagc
     4621 cacgttaggc ggccgctcga gatcagcctc gactgtgcct tctagttgcc agccatctgt
     4681 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     4741 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     4801 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     4861 tgcggtgggc tctatggctt ctgaggcgga aagaaccagt ggcggtaata cggttatcca
     4921 cagaatcggg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     4981 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     5041 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     5101 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5161 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     5221 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     5281 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacgcg
     5341 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     5401 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     5461 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     5521 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     5581 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     5641 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     5701 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taacctgagg
     5761 ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc tgggccttca cccgaacttg
     5821 gggggtgggg tggggaaaag gaagaaacgc gggcgtattg gccccaatgg ggtctcggtg
     5881 gggtatcgac agagtgccag ccctgggacc gaaccccgcg tttatgaaca aacgacccaa
     5941 caccgtgcgt tttattctgt ctttttattg ccgtcatagc gcgggttcct tccggtattg
     6001 tctccttccg tgtttcagtt agcctccccc tagggtgggc gaagaactcc agcatgagat
     6061 ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt
     6121 catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg
     6181 tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat
     6241 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
     6301 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac
     6361 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
     6421 caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc tcgccttgag
     6481 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc
     6541 gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc
     6601 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
     6661 tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa
     6721 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
     6781 cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc agttcattca gggcaccgga
     6841 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc
     6901 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
     6961 ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt
     7021 ctcttgatcg atctttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga
     7081 atagctcaga ggccgggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
     7141 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac
     7201 tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     7261 ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     7321 tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagct ggttctttcc
     7381 gcctcaggac tcttcctttt tcaataaatc aatctaaagt atatatgagt aaacttggtc
     7441 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7501 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7561 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7621 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7681 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7741 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7801 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7861 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7921 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7981 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8041 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8101 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8161 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8221 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8281 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8341 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8401 aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt
     8461 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     8521 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     8581 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     8641 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     8701 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     8761 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     8821 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     8881 aacgcttaca atttac
//
