LOCUS       sequence-474228-        1725 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid contains the secretion signal peptide sequence of the yeast 
            (S. uvarum) SPR1 gene for use in Combinatorial Golden Gate Assembly 
            (BsaI restriction sites)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Cleaver A, Luo R, Smith OB, Murphy L, Schwessinger B, Brock J
  TITLE     High-throughput optimisation of protein secretion in yeast via an 
            engineered biosensor.
  JOURNAL   Trends Biotechnol. 2025 Apr;43(4):838-867. doi: 
            10.1016/j.tibtech.2024.11.010. Epub 2024 Dec 13.
  PUBMED    39674781
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.tibtech.2024.11.010"; journalName: "Trends Biotechnol"; 
            date: "2025-04"; volume: "43"; issue: "4"; pages: "838-867"
COMMENT     Sequence Label: SP_A2
FEATURES             Location/Qualifiers
     source          1..1725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(90..124)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(195..854)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     769..788
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1036..1624
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1525..1544
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atggtgtcct tcagaggact gacgaccttg acactgcttt ttacgaaact
       61 ggttaattgc gccccttgag accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc
      121 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
      181 agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
      241 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
      301 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
      361 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgaa
      421 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
      481 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
      541 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
      601 catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg
      661 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
      721 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
      781 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
      841 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
      901 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaat
      961 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1021 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1081 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1141 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1201 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1261 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1321 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1381 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1441 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1501 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1561 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1621 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     1681 catgttcttt cctgcgttat cccctgattc tgtggataac cgtag
//
