LOCUS       sequence-474230-       14094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reports Cur activity in Bacteroides fragilis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14094)
  AUTHORS   Kabonick S, Verma K, Modesto JL, Pearce VH, Winokur KM, Groisman EA,
            Townsend GE
  TITLE     Hierarchical glycolytic pathways control the carbohydrate 
            utilization regulator in human gut Bacteroides.
  JOURNAL   bioRxiv [Preprint]. 2024 Nov 13:2024.11.13.623061. doi: 
            10.1101/2024.11.13.623061.
  PUBMED    39605394
REFERENCE   2  (bases 1 to 14094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Nov 13:2024.11.13.623061. doi: 
            10.1101/2024.11.13.623061."
COMMENT     Sequence Label: P-Bf-fusA2
FEATURES             Location/Qualifiers
     source          1..14094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     480..498
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(536..558)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     658..677
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3579..3601
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3593..3610
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3594..3610
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3993..5435
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             7624..8703
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLL"
     primer_bind     complement(9737..9753)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9737..9753)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9750..9772)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9761..9777
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9785..9791,9792..9809,9810..9815))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9830..9851
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9968..9985)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10139..10727)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10219..10238)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10898..11689,11690..11758))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11521..11540
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11759..11863)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attacacaaa aaaatatatc taacaaaagt aggaggattt tttaactttt taccatagac
       61 attttattag ggaaacgtag gttcaactgg taagtacaaa taagtagaaa tgtttgaaca
      121 acactgtttt aggaagttga aaaatcaagt ttctctctcg gtatagcgtt tattttggcc
      181 aatatcttct ttagtttggc atttgagtat ttcccattcg tgttctattt agctccttat
      241 tatgagtatg tagcaagttg cttatcaaat tcagcctctt tgagggtcaa atatggtttt
      301 gcaaatggtg actgacaaga cataaacaag gtcagcagga ttttttacat aaatggacat
      361 gaatgtatat aacggggttt gagggccaat ggaacgaaaa cgtacgttaa ggagataatt
      421 cgttgtttat atttaaattt agctaagaaa ccattattat catgacatta acctataaaa
      481 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
      541 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
      601 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
      661 catcagagca gattgtactg agagtgcacc attccccgac cgatgatttt taagtgactg
      721 attttgtgct gttttggggg tatattaaga ataaaagaaa tagaataagt taagtacttg
      781 atacacaata tagggcattt tccatattgg aaattctcat tttccaatct agaaaatacc
      841 gatttcctaa tatgatacta aataggaaaa taatatttcc cttaatattg tttttatgga
      901 aaataatagt ttactttgtg gagaataata tttcccaaaa acatatcaaa atggaaaata
      961 aaaaagcagt taagttaacc gattttcaaa agaacgaaga aaatcctttt atgaaacaag
     1021 ctatagaagg tattgaaaat catgttgtta aaaagtataa gagtaatagt ggtggcgata
     1081 agagagctgt agtagcttta gccgacactg aaactggaga agtgtttaag acttcgttta
     1141 tccgtcaaat agaagtagat gaagaacaat tcactaaatt gtatctttct aactttgctg
     1201 cattctttga cctatcacaa gcagctattc gggtttttgg ttactttatg acctgcatga
     1261 aacccaaaaa tgatttaatc attttcaata gaaaaaaatg cctagaatat accaaataca
     1321 aaacagacaa agccgtttat aaaggacttg cagaacttgt aaaagctgaa atcatagccc
     1381 gaggaccagc cgataatctt tggtttatta atcctctgat agtattcaat ggtgaccgag
     1441 tgacatttgc taaaacatac gttcggaaaa agactttagc tgcccaaaag aaagaagaag
     1501 cagagaaacg acaattatca cttggctttg atgaacagta acactccatt gagtgaagct
     1561 gccgtttggt cgctccccct ttgggggcgg ggggggatag ataaagttcc tctatgtaaa
     1621 gttataatgg gggatgaaag gcaaggttcg ctaaccttac cccgattgtt tatgcttccc
     1681 agcattcaat ctatccccgt ttatgggttt ctctttcacc ctacaaagat aacctcatgg
     1741 gggaaaaatg tccaagaata tggggtaaaa actatcaagt cggtagaaaa taagtatctt
     1801 tgaagtacat ttttagtaga ggtactggtg tgcctaggcg aagcagatag gcaaaaataa
     1861 agccgctgga gatgtcggat gtccgtcaat taactccagc ggcttgtatt ttggtaactc
     1921 tcccgtatcg gtcagctacc gtttggtcag caacaaaggt agtactttat tatcgcaaat
     1981 ccaaatgttt ttccctttat ttgtgcattt acaaaacatt cactaacttc gcacctatgc
     2041 agtatggtga tagcacacta gcaccctaaa gagtgcgtgt gtaaggggac aagccccctt
     2101 aaccccctgt cagccggctg tcaccggctg ttatcgattg tcacaaaaat gttaacttat
     2161 gggagcaaca agtattcatg tacaagcagt gaagccgggg agcgagattc acaactttag
     2221 ggaaaaagag ttggactatg ttcgtcccga acttagtcat ttgaatgaaa gctgggttgg
     2281 agatagcatt tcccatcggc tggagagtgc aaaacaaaga tatctcgata cggttgggca
     2341 gaagatgcag gctaaagccg cacccatacg agagggagta atagtaatca aacaagaaac
     2401 cactatgcag gaactccagc agtttgccac ggtctgcaaa gaacgtttcg gtatcgaagc
     2461 gtttcaaatc catatacaca aggacgaagg atacatgaac gcaaagcagt ggacacctaa
     2521 ccttcatgcc catgtagttt tcgattggac gcagccgaat gggaaaagtg tgcgtttatc
     2581 gcgtgatgac atggcagaac tccaaaccat agcatctgaa acactgggca tggaacgtgg
     2641 cgtttcttct gaccgcaaac acttatcggc tatgcagtac aagaccgaat gtgcgaaaga
     2701 acagcttcag gaactatcca acgatatatc cagtgcatta gacaaataca aggacgcaca
     2761 aaaccagctt cttcagcttc agaaggaact acgttccatt gaaacaaaga agaacgtcca
     2821 aaaactcatt tctaaggctt cagagaagtt ttacggcttg attggaaaaa cagtcaacga
     2881 tagggaaaag gatgccttaa aagccaaagt aaagacttta gagggggaaa atgaacaact
     2941 atccgataga ctgggaaagg ctatacttga aaaagagcga aacagcacaa aggcattcaa
     3001 agccgagaat gacaaggagt attatagaca acaaatggac aacgcaagga caaccagtaa
     3061 cctattaagg acggagaacc aaaaactaaa agccgaaacc aaggagttga aaaaagaact
     3121 tggtaagatg aaagacttgt tcaattccga acaactggag gctttaaggc atcatttccc
     3181 aaacatatca aaagctatgg aagaggggaa agacctattc aagcaaatca ccagaagcag
     3241 aggtttcggc atgggtatgt aacccccccc cgcccccaaa gggggagcga ccaaacggca
     3301 gcctctctca atggagtgtt acaccgttcg gattcagaaa acattttgca gggttcggaa
     3361 aacggttgtg tgcttattct aaaaaaagtt tatggtgtgt atgcggtgtg aaataccgca
     3421 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
     3481 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     3541 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     3601 gacggccagt gaattcgagc tcggtacccg gggatccgaa aaacctccgt tttgcaccga
     3661 aaagtctgct gctgcattct atttcatcct gtccggaaga agaaggagtc agtaaatgta
     3721 aggcaaactg ctctttttaa tcgggaatcg gaaagaatac ttccattctg tcagatagcc
     3781 tgtttcgcgc ttgtcgaagg ctgtatgtta cataacattg tttagtaaca ctgtccgttt
     3841 tattgctttt catttgatta atgcctaatt tgcgcctcag taaaaaaagg gtatgcgaaa
     3901 aacacatact gaatcattca tttaaatctt atatatcact agtcactccc gcattttaaa
     3961 ataaaataaa ttatttattt aattaaacga atatgactaa aaaaatttca ttcattatta
     4021 acggccaggt tgaaatcttt cccgaaggtg atgatttagt gcaatccatt aattttggtg
     4081 ataatagtgt ttacctgcca atattgaatg actctcatgt aaaaaacatt attgattgta
     4141 atggaaataa cgaattacgg ttgcataaca ttgtcaattt tctctatacg gtagggcaaa
     4201 gatggaaaaa tgaagaatac tcaagacgca ggacatacat tcgtgactta aaaaaatata
     4261 tgggatattc agaagaaatg gctaagctag aggccaattg gatatctatg attttatgtt
     4321 ctaaaggcgg cctttatgat gttgtagaaa atgaacttgg ttctcgccat atcatggatg
     4381 aatggctacc tcaggatgaa agttatgttc gggcttttcc gaaaggtaaa tctgtacatc
     4441 tgttggcagg taatgttcca ttatctggga tcatgtctat attacgcgca attttaacta
     4501 agaatcagtg tattataaaa acatcgtcaa ccgatccttt taccgctaat gcattagcgt
     4561 taagttttat tgatgtagac cctaatcatc cgataacgcg ctctttatct gttatatatt
     4621 ggccccacca aggtgataca tcactcgcaa aagaaattat gcaacatgcg gatgttattg
     4681 tcgcttgggg agggccagat gcgattaatt gggcggtaga gcatgcgcca tcttatgctg
     4741 atgtgattaa atttggttct aaaaagagtc tttgcattat cgataatcct gttgatttga
     4801 cgtccgcagc gacaggtgcg gctcatgatg tttgttttta cgatcagcga gcttgttttt
     4861 ctgcccaaaa catatattac atgggaaatc attatgagga atttaagtta gcgttgatag
     4921 aaaaacttaa tctatatgcg catatattac cgaatgccaa aaaagatttt gatgaaaagg
     4981 cggcctattc tttagttcaa aaagaaagct tgtttgctgg attaaaagta gaggtggata
     5041 ttcatcaacg ttggatgatt attgagtcaa atgcaggtgt ggaatttaat caaccacttg
     5101 gcagatgtgt gtaccttcat cacgtcgata atattgagca aatattgcct tatgttcaaa
     5161 aaaataagac gcaaaccata tctatttttc cttgggagtc atcatttaaa tatcgagatg
     5221 cgttagcatt aaaaggtgcg gaaaggattg tagaagcagg aatgaataac atatttcgag
     5281 ttggtggatc tcatgacgga atgagaccgt tgcaacgatt agtgacatat atttctcatg
     5341 aaaggccatc taactatacg gctaaggatg ttgcggttga aatagaacag actcgattcc
     5401 tggaagaaga taagttcctt gtatttgtcc cgtaatccgc atggcaaatt gtaaattgta
     5461 aatcgtaaaa tagtaatata ttaatggaaa ataaatccaa atataaaacc atcgaccacg
     5521 ttatttgtgt tgaaggaaat aaaaaaattc atgtttggga aacgctgcca gaagaaaaca
     5581 gcccaaagag aaagaatgcc attattattg cgtctggttt tgcccgcagg atggatcatt
     5641 ttgctggtct ggcggaatat ttatcgcgga atggatttca tgtgatccgc tatgattcgc
     5701 ttcaccacgt tggattgagt tcagggacaa ttgatgaatt tacaatgtct ataggaaagc
     5761 agagcttgtt agcagtggtt gattggttaa ctacacgaaa aataaataac ttcggtatgt
     5821 tggcttcaag cttatctgcg cggatagctt atgcaagcct atctgaaatc aatgcttcgt
     5881 ttttaatcac cgcagtcggt gttgttaact taagatattc tcttgaaaga gctttagggt
     5941 ttgattatct cagtctaccc attaatgaat tgccgaataa tctagatttt gaaggccata
     6001 aattgggtgc tgaagtcttt gcgagagatt gtcttgattt tggttgggaa gatttagctt
     6061 ctacaattaa taacatgatg tatcttgata taccgtttat tgcttttact gcaaataacg
     6121 ataattgggt caagcaagat gaagttatca cattgttatc aaatattcgt agtaatcgat
     6181 gcaagatata ttctttgtta ggaagttcgc atgacttgag tgaaaattta gtggtcctgc
     6241 gcaattttta tcaatcggtt acgaaagccg ctatcgcgat ggataatgat catctggata
     6301 ttgatgttga tattactgaa ccgtcatttg aacatttaac tattgcgaca gtcaatgaac
     6361 gccgaatgag aattgagatt gaaaatcaag cgatttcgct gtcttaataa ggaggaggat
     6421 aaagaatatg acttcatatg ttgataaaca agaaattaca gcaagctcag aaattgatga
     6481 tttgattttt tcgagcgatc cattagtgtg gtcttacgac gagcaggaaa aaatcagaaa
     6541 gaaacttgtg cttgatgcat ttcgtaatca ttataaacat tgtcgagaat atcgtcacta
     6601 ctgtcaggca cacaaagtag atgacaatat tacggaaatt gatgacatac ctgtattccc
     6661 aacatcggtt tttaagttta ctcgcttatt aacttctcag gaaaacgaga ttgaaagttg
     6721 gtttaccagt agcggcacga atggtttaaa aagtcaggtg gcgcgtgaca gattaagtat
     6781 tgagagactc ttaggctctg tgagttatgg catgaaatat gttggtagtt ggtttgatca
     6841 tcaaatagaa ttagtcaatt tgggaccaga tagatttaat gctcataata tttggtttaa
     6901 atatgttatg agtttggtgg aattgttata tcctacgaca tttaccgtaa cagaagaacg
     6961 aatagatttt gttaaaacat tgaatagtct tgaacgaata aaaaatcaag ggaaagatct
     7021 ttgtcttatt ggttcgccat actttattta tttactctgc cattatatga aagataaaaa
     7081 aatctcattt tctggagata aaagccttta tatcataacc ggaggcggct ggaaaagtta
     7141 cgaaaaagaa tctctgaaac gtgatgattt caatcatctt ttatttgata ctttcaatct
     7201 cagtgatatt agtcagatcc gagatatatt taatcaagtt gaactcaaca cttgtttctt
     7261 tgaggatgaa atgcagcgta aacatgttcc gccgtgggta tatgcgcgag cgcttgatcc
     7321 tgaaacgttg aaacctgtac ctgatggaac gccggggttg atgagttata tggatgcgtc
     7381 agcaaccagt tatccagcat ttattgttac cgatgatgtc gggataatta gcagagaata
     7441 tggtaagtat cccggcgtgc tcgttgaaat tttacgtcgc gtcaatacga ggacgcagaa
     7501 agggtgtgct ttaagcttaa accaagcatt taatagttga tataaatgaa gggtgaagaa
     7561 ggatgaatga aggtaacact cataaactcg aaattcttca ttcttaattt ttaattaaaa
     7621 tatatgaaat ttggaaactt tttgcttaca taccaacctc cccaattttc tcaaacagag
     7681 gtaatgaaac gtttggttaa attaggtcgc atctctgagg agtgtggttt tgataccgta
     7741 tggttactgg agcatcattt cacggagttt ggtttgcttg gtaaccctta tgtcgctgct
     7801 gcatatttac ttggcgcgac taaaaaattg aatgtaggaa ctgccgctat tgttcttccc
     7861 acagcccatc cagtacgcca acttgaagat gtgaatttat tggatcaaat gtcaaaagga
     7921 cgatttcggt ttggtatttg ccgagggctt tacaacaagg actttcgcgt attcggcaca
     7981 gatatgaata acagtcgcgc cttagcggaa tgctggtacg ggctgataaa gaatggcatg
     8041 acagagggat atatggaagc tgataatgaa catatcaagt tccataaggt aaaagtaaac
     8101 cccgcggcgt atagcagagg tggcgcaccg gtttatgtgg tggctgaatc agcttcgacg
     8161 actgagtggg ctgctcaatt tggcctaccg atgatattaa gttggattat aaatactaac
     8221 gaaaagaaag cacaacttga gctttataat gaagtggctc aagaatatgg gcacgatatt
     8281 cataatatcg accattgctt atcatatata acatctgtag atcatgactc aattaaagcg
     8341 aaagagattt gccggaaatt tctggggcat tggtatgatt cttatgtgaa tgctacgact
     8401 atttttgatg attcagacca aacaagaggt tatgatttca ataaagggca gtggcgtgac
     8461 tttgtattaa aaggacataa agatactaat cgccgtattg attacagtta cgaaatcaat
     8521 cccgtgggaa cgccgcagga atgtattgac ataattcaaa aagacattga tgctacagga
     8581 atatcaaata tttgttgtgg atttgaagct aatggaacag tagacgaaat tattgcttcc
     8641 atgaagctct tccagtctga tgtcatgcca tttcttaaag aaaaacaacg ttcgctatta
     8701 taaagaaagg aataagacaa tggatatgaa atttggattg ttcttcctta acttcatcaa
     8761 ttcaacaact gttcaagaac aaagtatagt tcgcatgcag gaaataacgg agtatgttga
     8821 taagttgaat tttgaacaga ttttagtgta tgaaaatcat ttttcagata atggtgttgt
     8881 cggcgctcct ctgactgttt ctggttttct gctcggttta acagagaaaa ttaaaattgg
     8941 ttcattaaat cacatcatta caactcatca tcctgtccgc atagcggagg aagcttgctt
     9001 attggatcag ttaagtgaag ggagatttat tttagggttt agtgattgcg aaaaaaaaga
     9061 tgaaatgcat ttttttaatc gcccggttga atatcaacag caactatttg aagagtgtta
     9121 tgaaatcatt aacgatgctt taacaacagg ctattgtaat ccagataacg atttttatag
     9181 cttccctaaa atatctgtaa atccccatgc ttatacgcca ggcggacctc ggaaatatgt
     9241 aacagcaacc agtcatcata ttgttgagtg ggcggccaaa aaaggtattc ctctcatctt
     9301 taagtgggat gattctaatg atgttagata tgaatatgct gaaagatata aagccgttgc
     9361 ggataaatat gacgttgacc tatcagagat agaccatcag ttaatgatat tagttaacta
     9421 taacgaagat agtaataaag ctaaacaaga gacgcgtgca tttattagtg attatgttct
     9481 tgaaatgcac cctaatgaaa atttcgaaaa taaacttgaa gaaataattg cagaaaacgc
     9541 tgtcggaaat tatacggagt gtataactgc ggctaagttg gcaattgaaa agtgtggtgc
     9601 gaaaagtgta ttgctgtcct ttgaaccaat gaatgatttg atgagccaaa aaaatgtaat
     9661 caatattgtt gatgataata ttaaaaagta ccacatgtaa gtcgacctgc aggcatgcaa
     9721 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     9781 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     9841 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     9901 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     9961 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
    10021 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
    10081 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    10141 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    10201 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    10261 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    10321 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    10381 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    10441 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    10501 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    10561 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
    10621 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    10681 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    10741 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    10801 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    10861 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    10921 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    10981 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    11041 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    11101 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    11161 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    11221 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    11281 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    11341 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    11401 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    11461 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    11521 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    11581 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    11641 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    11701 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    11761 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    11821 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    11881 tgccacctgc gtctattttt ttattgccaa gttctaatgc ttctatctcc tgccattcat
    11941 agaggctatt atatgccata catccgataa gaatggagat aagtatatat cccaactgta
    12001 ttgccttata gaaatttcct gaccgctcca ttattttgat gacattgatt tttggaacat
    12061 gaataaaagt ttatcttttt cgttcatgcg gatattatca gaataaccgc cttttgttat
    12121 ttgatacccg catggcttaa ccataaaaat gcctaagccc ttagtgtatg aacttacttc
    12181 tgatgcatag tctttacttg tatttaatgg aactttccct ttaatatgac accactcatt
    12241 attgcaactg atgtcttcaa tctcagacat cattttttgc aaatctgtaa tagctttgga
    12301 acttgccgct tggggtatct gcaactcaaa atagagcatc ggttcgagaa tgtccacacc
    12361 tgactgttgc aaggccagcc tgaagacata aggggtcagc tgtctgaaat cagcaggtgt
    12421 acttaccggg ctataatact cggcttgagt aaaagttact ttcagatcag tcacttccca
    12481 tccatgtaac ccggattggc aagacatacg aatcccttca aaaacggcat tttgaaaaga
    12541 atggttcaga taaccatagg agatgtcact ttcgatttgc aaccctgtcc ctaacggtaa
    12601 gggttcaaga gtcagcccta ttgtggccca ataagggttg ggcggcactt cgatctgaat
    12661 aatcttattg acctttttta caggtcgttc tttgtatata gtcttgatct catcaaaatg
    12721 gacctttacg gaaaatcgtt cttccagcaa tgtctgtatg atttcctttt gggttaaacc
    12781 atataacgag atttccaatt catcactata tgagtttatg gaaaaggaca aagacgggtc
    12841 ttcaatccac aatgtattca gagcggatat caccttgctt ctctcttcgg gcctgtctgg
    12901 ccggacggag gatttgagag cgggatgctg atgcgataat ccttgaatca aacaaggttc
    12961 agcacctaaa taatttccga ttcgaaaatc atccatatcc tctacaatcg cgatatcatt
    13021 ggcgcccact tcatcaacat ttatctctct gccctgattg atagttttta gatttttaat
    13081 cttgatgaat ttttccgaat cgttgattct tacaacgtct cgaagtctca gacttccgtc
    13141 aattattttt agaaaacttc ttttatgtcc tttggggtca tgctctatct tataaagata
    13201 agatgaaagt ctgtttgaga ccgatgccgg aggaagtata aaagaagtga tggcgtccaa
    13261 caactcattg ataccgatat tgaacattgc tgatccatgt agcaccggat agactttggc
    13321 ttttgccaca agagcgatta tcgtattcca ataatcagcc ggtgaaattt cgctatccgc
    13381 caaatatcgt tctaatatat tgtcgtcatg gttgcataca aattctttgt attcttcctt
    13441 tatatatgtt tgggagcaaa ccggataaac cgatccatcg acaacatttt gcataaacag
    13501 gacatcttga gacagatttg cttttatatc cagatacaaa cgctccaaat tcacaccggc
    13561 tcggtcaatc ttattgataa atataattgt cgggatttgc agcttctgta aagtattgaa
    13621 cagcaacttt gtctgcgctt gtatgccttc ctttgcggat aagatgagga ctgctccatc
    13681 aagcattttg aatgtccgct ccacttccgc aataaaatcc atgtgtcccg gagtgtcaat
    13741 gatattgcat ttcacaccat tccagataat agatgtcgta gaagcccgaa cagtaattcc
    13801 tctacgtttc tctatatcca tagagtccgt tatggtgtca ccattatcca cacagccgca
    13861 cttttccgtt gctccactgg caaacagcag attctcggtt acggaagttt ttcctgcatc
    13921 aatgtgagca agaattccta aatttataat attcatttgg attaagcaat aatatactac
    13981 aatagatgca ttgtcgaaac gcacctttta atacctcctc gtagcatatg agaactacag
    14041 gattcctaac tcgtattaat atgtatatta ttactgccgc ataacgatta caaa
//
