LOCUS       sequence-474231-       14072 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  B. thetaiotaomicron fusA2 expression reporter lacking 22bp Cur 
            binding site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14072)
  AUTHORS   Kabonick S, Verma K, Modesto JL, Pearce VH, Winokur KM, Groisman EA,
            Townsend GE
  TITLE     Hierarchical glycolytic pathways control the carbohydrate 
            utilization regulator in human gut Bacteroides.
  JOURNAL   bioRxiv [Preprint]. 2024 Nov 13:2024.11.13.623061. doi: 
            10.1101/2024.11.13.623061.
  PUBMED    39605394
REFERENCE   2  (bases 1 to 14072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Nov 13:2024.11.13.623061. doi: 
            10.1101/2024.11.13.623061."
COMMENT     Sequence Label: P-d22bp
FEATURES             Location/Qualifiers
     source          1..14072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2066..3508)
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     primer_bind     complement(3869..3886)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3869..3885)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3878..3900)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6802..6821)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6921..6943
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6981..6999)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9710..9814
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9815..9883,9884..10675)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10033..10052)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10846..11434
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11335..11354
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11588..11605
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11722..11743
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11758..11763,11764..11781,11782..11788)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11796..11812
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11801..11823
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11820..11836
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11820..11836
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(12870..13949)
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLL"
ORIGIN
        1 ttctgcgtcc tcgtattgac gcgacgtaaa atttcaacga gcacgccggg atacttacca
       61 tattctctgc taattatccc gacatcatcg gtaacaataa atgctggata actggttgct
      121 gacgcatcca tataactcat caaccccggc gttccatcag gtacaggttt caacgtttca
      181 ggatcaagcg ctcgcgcata tacccacggc ggaacatgtt tacgctgcat ttcatcctca
      241 aagaaacaag tgttgagttc aacttgatta aatatatctc ggatctgact aatatcactg
      301 agattgaaag tatcaaataa aagatgattg aaatcatcac gtttcagaga ttctttttcg
      361 taacttttcc agccgcctcc ggttatgata taaaggcttt tatctccaga aaatgagatt
      421 tttttatctt tcatataatg gcagagtaaa taaataaagt atggcgaacc aataagacaa
      481 agatctttcc cttgattttt tattcgttca agactattca atgttttaac aaaatctatt
      541 cgttcttctg ttacggtaaa tgtcgtagga tataacaatt ccaccaaact cataacatat
      601 ttaaaccaaa tattatgagc attaaatcta tctggtccca aattgactaa ttctatttga
      661 tgatcaaacc aactaccaac atatttcatg ccataactca cagagcctaa gagtctctca
      721 atacttaatc tgtcacgcgc cacctgactt tttaaaccat tcgtgccgct actggtaaac
      781 caactttcaa tctcgttttc ctgagaagtt aataagcgag taaacttaaa aaccgatgtt
      841 gggaatacag gtatgtcatc aatttccgta atattgtcat ctactttgtg tgcctgacag
      901 tagtgacgat attctcgaca atgtttataa tgattacgaa atgcatcaag cacaagtttc
      961 tttctgattt tttcctgctc gtcgtaagac cacactaatg gatcgctcga aaaaatcaaa
     1021 tcatcaattt ctgagcttgc tgtaatttct tgtttatcaa catatgaagt catattcttt
     1081 atcctcctcc ttattaagac agcgaaatcg cttgattttc aatctcaatt ctcattcggc
     1141 gttcattgac tgtcgcaata gttaaatgtt caaatgacgg ttcagtaata tcaacatcaa
     1201 tatccagatg atcattatcc atcgcgatag cggctttcgt aaccgattga taaaaattgc
     1261 gcaggaccac taaattttca ctcaagtcat gcgaacttcc taacaaagaa tatatcttgc
     1321 atcgattact acgaatattt gataacaatg tgataacttc atcttgcttg acccaattat
     1381 cgttatttgc agtaaaagca ataaacggta tatcaagata catcatgtta ttaattgtag
     1441 aagctaaatc ttcccaacca aaatcaagac aatctctcgc aaagacttca gcacccaatt
     1501 tatggccttc aaaatctaga ttattcggca attcattaat gggtagactg agataatcaa
     1561 accctaaagc tctttcaaga gaatatctta agttaacaac accgactgcg gtgattaaaa
     1621 acgaagcatt gatttcagat aggcttgcat aagctatccg cgcagataag cttgaagcca
     1681 acataccgaa gttatttatt tttcgtgtag ttaaccaatc aaccactgct aacaagctct
     1741 gctttcctat agacattgta aattcatcaa ttgtccctga actcaatcca acgtggtgaa
     1801 gcgaatcata gcggatcaca tgaaatccat tccgcgataa atattccgcc agaccagcaa
     1861 aatgatccat cctgcgggca aaaccagacg caataataat ggcattcttt ctctttgggc
     1921 tgttttcttc tggcagcgtt tcccaaacat gaattttttt atttccttca acacaaataa
     1981 cgtggtcgat ggttttatat ttggatttat tttccattaa tatattacta ttttacgatt
     2041 tacaatttac aatttgccat gcggattacg ggacaaatac aaggaactta tcttcttcca
     2101 ggaatcgagt ctgttctatt tcaaccgcaa catccttagc cgtatagtta gatggccttt
     2161 catgagaaat atatgtcact aatcgttgca acggtctcat tccgtcatga gatccaccaa
     2221 ctcgaaatat gttattcatt cctgcttcta caatcctttc cgcacctttt aatgctaacg
     2281 catctcgata tttaaatgat gactcccaag gaaaaataga tatggtttgc gtcttatttt
     2341 tttgaacata aggcaatatt tgctcaatat tatcgacgtg atgaaggtac acacatctgc
     2401 caagtggttg attaaattcc acacctgcat ttgactcaat aatcatccaa cgttgatgaa
     2461 tatccacctc tacttttaat ccagcaaaca agctttcttt ttgaactaaa gaataggccg
     2521 ccttttcatc aaaatctttt ttggcattcg gtaatatatg cgcatataga ttaagttttt
     2581 ctatcaacgc taacttaaat tcctcataat gatttcccat gtaatatatg ttttgggcag
     2641 aaaaacaagc tcgctgatcg taaaaacaaa catcatgagc cgcacctgtc gctgcggacg
     2701 tcaaatcaac aggattatcg ataatgcaaa gactcttttt agaaccaaat ttaatcacat
     2761 cagcataaga tggcgcatgc tctaccgccc aattaatcgc atctggccct ccccaagcga
     2821 caataacatc cgcatgttgc ataatttctt ttgcgagtga tgtatcacct tggtggggcc
     2881 aatatataac agataaagag cgcgttatcg gatgattagg gtctacatca ataaaactta
     2941 acgctaatgc attagcggta aaaggatcgg ttgacgatgt ttttataata cactgattct
     3001 tagttaaaat tgcgcgtaat atagacatga tcccagataa tggaacatta cctgccaaca
     3061 gatgtacaga tttacctttc ggaaaagccc gaacataact ttcatcctga ggtagccatt
     3121 catccatgat atggcgagaa ccaagttcat tttctacaac atcataaagg ccgcctttag
     3181 aacataaaat catagatatc caattggcct ctagcttagc catttcttct gaatatccca
     3241 tatatttttt taagtcacga atgtatgtcc tgcgtcttga gtattcttca tttttccatc
     3301 tttgccctac cgtatagaga aaattgacaa tgttatgcaa ccgtaattcg ttatttccat
     3361 tacaatcaat aatgtttttt acatgagagt cattcaatat tggcaggtaa acactattat
     3421 caccaaaatt aatggattgc actaaatcat caccttcggg aaagatttca acctggccgt
     3481 taataatgaa tgaaattttt ttagtcatat tcgtttaatt aaataaataa tttattttat
     3541 tttaaaatgc gggagtgact agtaatatat aagattttag taattactca gtatgtttct
     3601 cgcataccat taatttagtg atgcgcaaat tagggattta ttgggggaaa agcaatattt
     3661 atgacagtgt tactatccgt tttatgggta gcagacaggc cagacttata cctaattccc
     3721 gttaattgta tcaattgctc agaaaaaagg tttaaatttg caggctataa tcaattggta
     3781 acaagctaaa catctactat ggcaggtatt taccttcata ttcccttctg taagacacgt
     3841 tggatccccg ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt cgtgactggg
     3901 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     3961 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     4021 aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     4081 acaccataaa ctttttttag aataagcaca caaccgtttt ccgaaccctg caaaatgttt
     4141 tctgaatccg aacggtgtaa cactccattg agagaggctg ccgtttggtc gctccccctt
     4201 tgggggcggg ggggggttac atacccatgc cgaaacctct gcttctggtg atttgcttga
     4261 ataggtcttt cccctcttcc atagcttttg atatgtttgg gaaatgatgc cttaaagcct
     4321 ccagttgttc ggaattgaac aagtctttca tcttaccaag ttcttttttc aactccttgg
     4381 tttcggcttt tagtttttgg ttctccgtcc ttaataggtt actggttgtc cttgcgttgt
     4441 ccatttgttg tctataatac tccttgtcat tctcggcttt gaatgccttt gtgctgtttc
     4501 gctctttttc aagtatagcc tttcccagtc tatcggatag ttgttcattt tccccctcta
     4561 aagtctttac tttggctttt aaggcatcct tttccctatc gttgactgtt tttccaatca
     4621 agccgtaaaa cttctctgaa gccttagaaa tgagtttttg gacgttcttc tttgtttcaa
     4681 tggaacgtag ttccttctga agctgaagaa gctggttttg tgcgtccttg tatttgtcta
     4741 atgcactgga tatatcgttg gatagttcct gaagctgttc tttcgcacat tcggtcttgt
     4801 actgcatagc cgataagtgt ttgcggtcag aagaaacgcc acgttccatg cccagtgttt
     4861 cagatgctat ggtttggagt tctgccatgt catcacgcga taaacgcaca cttttcccat
     4921 tcggctgcgt ccaatcgaaa actacatggg catgaaggtt aggtgtccac tgctttgcgt
     4981 tcatgtatcc ttcgtccttg tgtatatgga tttgaaacgc ttcgataccg aaacgttctt
     5041 tgcagaccgt ggcaaactgc tggagttcct gcatagtggt ttcttgtttg attactatta
     5101 ctccctctcg tatgggtgcg gctttagcct gcatcttctg cccaaccgta tcgagatatc
     5161 tttgttttgc actctccagc cgatgggaaa tgctatctcc aacccagctt tcattcaaat
     5221 gactaagttc gggacgaaca tagtccaact ctttttccct aaagttgtga atctcgctcc
     5281 ccggcttcac tgcttgtaca tgaatacttg ttgctcccat aagttaacat ttttgtgaca
     5341 atcgataaca gccggtgaca gccggctgac agggggttaa gggggcttgt ccccttacac
     5401 acgcactctt tagggtgcta gtgtgctatc accatactgc ataggtgcga agttagtgaa
     5461 tgttttgtaa atgcacaaat aaagggaaaa acatttggat ttgcgataat aaagtactac
     5521 ctttgttgct gaccaaacgg tagctgaccg atacgggaga gttaccaaaa tacaagccgc
     5581 tggagttaat tgacggacat ccgacatctc cagcggcttt atttttgcct atctgcttcg
     5641 cctaggcaca ccagtacctc tactaaaaat gtacttcaaa gatacttatt ttctaccgac
     5701 ttgatagttt ttaccccata ttcttggaca tttttccccc atgaggttat ctttgtaggg
     5761 tgaaagagaa acccataaac ggggatagat tgaatgctgg gaagcataaa caatcggggt
     5821 aaggttagcg aaccttgcct ttcatccccc attataactt tacatagagg aactttatct
     5881 atcccccccc gcccccaaag ggggagcgac caaacggcag cttcactcaa tggagtgtta
     5941 ctgttcatca aagccaagtg ataattgtcg tttctctgct tcttctttct tttgggcagc
     6001 taaagtcttt ttccgaacgt atgttttagc aaatgtcact cggtcaccat tgaatactat
     6061 cagaggatta ataaaccaaa gattatcggc tggtcctcgg gctatgattt cagcttttac
     6121 aagttctgca agtcctttat aaacggcttt gtctgttttg tatttggtat attctaggca
     6181 tttttttcta ttgaaaatga ttaaatcatt tttgggtttc atgcaggtca taaagtaacc
     6241 aaaaacccga atagctgctt gtgataggtc aaagaatgca gcaaagttag aaagatacaa
     6301 tttagtgaat tgttcttcat ctacttctat ttgacggata aacgaagtct taaacacttc
     6361 tccagtttca gtgtcggcta aagctactac agctctctta tcgccaccac tattactctt
     6421 atacttttta acaacatgat tttcaatacc ttctatagct tgtttcataa aaggattttc
     6481 ttcgttcttt tgaaaatcgg ttaacttaac tgctttttta ttttccattt tgatatgttt
     6541 ttgggaaata ttattctcca caaagtaaac tattattttc cataaaaaca atattaaggg
     6601 aaatattatt ttcctattta gtatcatatt aggaaatcgg tattttctag attggaaaat
     6661 gagaatttcc aatatggaaa atgccctata ttgtgtatca agtacttaac ttattctatt
     6721 tcttttattc ttaatatacc cccaaaacag cacaaaatca gtcacttaaa aatcatcggt
     6781 cggggaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     6841 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     6901 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     6961 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     7021 aataatggtt tcttagctaa atttaaatat aaacaacgaa ttatctcctt aacgtacgtt
     7081 ttcgttccat tggccctcaa accccgttat atacattcat gtccatttat gtaaaaaatc
     7141 ctgctgacct tgtttatgtc ttgtcagtca ccatttgcaa aaccatattt gaccctcaaa
     7201 gaggctgaat ttgataagca acttgctaca tactcataat aaggagctaa atagaacacg
     7261 aatgggaaat actcaaatgc caaactaaag aagatattgg ccaaaataaa cgctataccg
     7321 agagagaaac ttgatttttc aacttcctaa aacagtgttg ttcaaacatt tctacttatt
     7381 tgtacttacc agttgaacct acgtttccct aataaaatgt ctatggtaaa aagttaaaaa
     7441 atcctcctac ttttgttaga tatatttttt tgtgtaattt tgtaatcgtt atgcggcagt
     7501 aataatatac atattaatac gagttaggaa tcctgtagtt ctcatatgct acgaggaggt
     7561 attaaaaggt gcgtttcgac aatgcatcta ttgtagtata ttattgctta atccaaatga
     7621 atattataaa tttaggaatt cttgctcaca ttgatgcagg aaaaacttcc gtaaccgaga
     7681 atctgctgtt tgccagtgga gcaacggaaa agtgcggctg tgtggataat ggtgacacca
     7741 taacggactc tatggatata gagaaacgta gaggaattac tgttcgggct tctacgacat
     7801 ctattatctg gaatggtgtg aaatgcaata tcattgacac tccgggacac atggatttta
     7861 ttgcggaagt ggagcggaca ttcaaaatgc ttgatggagc agtcctcatc ttatccgcaa
     7921 aggaaggcat acaagcgcag acaaagttgc tgttcaatac tttacagaag ctgcaaatcc
     7981 cgacaattat atttatcaat aagattgacc gagccggtgt gaatttggag cgtttgtatc
     8041 tggatataaa agcaaatctg tctcaagatg tcctgtttat gcaaaatgtt gtcgatggat
     8101 cggtttatcc ggtttgctcc caaacatata taaaggaaga atacaaagaa tttgtatgca
     8161 accatgacga caatatatta gaacgatatt tggcggatag cgaaatttca ccggctgatt
     8221 attggaatac gataatcgct cttgtggcaa aagccaaagt ctatccggtg ctacatggat
     8281 cagcaatgtt caatatcggt atcaatgagt tgttggacgc catcacttct tttatacttc
     8341 ctccggcatc ggtctcaaac agactttcat cttatcttta taagatagag catgacccca
     8401 aaggacataa aagaagtttt ctaaaaataa ttgacggaag tctgagactt cgagacgttg
     8461 taagaatcaa cgattcggaa aaattcatca agattaaaaa tctaaaaact atcaatcagg
     8521 gcagagagat aaatgttgat gaagtgggcg ccaatgatat cgcgattgta gaggatatgg
     8581 atgattttcg aatcggaaat tatttaggtg ctgaaccttg tttgattcaa ggattatcgc
     8641 atcagcatcc cgctctcaaa tcctccgtcc ggccagacag gcccgaagag agaagcaagg
     8701 tgatatccgc tctgaataca ttgtggattg aagacccgtc tttgtccttt tccataaact
     8761 catatagtga tgaattggaa atctcgttat atggtttaac ccaaaaggaa atcatacaga
     8821 cattgctgga agaacgattt tccgtaaagg tccattttga tgagatcaag actatataca
     8881 aagaacgacc tgtaaaaaag gtcaataaga ttattcagat cgaagtgccg cccaaccctt
     8941 attgggccac aatagggctg actcttgaac ccttaccgtt agggacaggg ttgcaaatcg
     9001 aaagtgacat ctcctatggt tatctgaacc attcttttca aaatgccgtt tttgaaggga
     9061 ttcgtatgtc ttgccaatcc gggttacatg gatgggaagt gactgatctg aaagtaactt
     9121 ttactcaagc cgagtattat agcccggtaa gtacacctgc tgatttcaga cagctgaccc
     9181 cttatgtctt caggctggcc ttgcaacagt caggtgtgga cattctcgaa ccgatgctct
     9241 attttgagtt gcagataccc caagcggcaa gttccaaagc tattacagat ttgcaaaaaa
     9301 tgatgtctga gattgaagac atcagttgca ataatgagtg gtgtcatatt aaagggaaag
     9361 ttccattaaa tacaagtaaa gactatgcat cagaagtaag ttcatacact aagggcttag
     9421 gcatttttat ggttaagcca tgcgggtatc aaataacaaa aggcggttat tctgataata
     9481 tccgcatgaa cgaaaaagat aaacttttat tcatgttcca aaaatcaatg tcatcaaaat
     9541 aatggagcgg tcaggaaatt tctataaggc aatacagttg ggatatatac ttatctccat
     9601 tcttatcgga tgtatggcat ataatagcct ctatgaatgg caggagatag aagcattaga
     9661 acttggcaat aaaaaaatag acgcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     9721 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     9781 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     9841 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     9901 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     9961 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
    10021 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
    10081 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
    10141 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
    10201 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
    10261 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
    10321 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
    10381 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
    10441 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
    10501 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
    10561 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    10621 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    10681 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    10741 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    10801 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    10861 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    10921 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    10981 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    11041 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    11101 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    11161 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    11221 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    11281 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    11341 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    11401 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    11461 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
    11521 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
    11581 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
    11641 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
    11701 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
    11761 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
    11821 aggaaacagc tatgaccatg attacgccaa gcttgcatgc ctgcaggtcg acttacatgt
    11881 ggtacttttt aatattatca tcaacaatat tgattacatt tttttggctc atcaaatcat
    11941 tcattggttc aaaggacagc aatacacttt tcgcaccaca cttttcaatt gccaacttag
    12001 ccgcagttat acactccgta taatttccga cagcgttttc tgcaattatt tcttcaagtt
    12061 tattttcgaa attttcatta gggtgcattt caagaacata atcactaata aatgcacgcg
    12121 tctcttgttt agctttatta ctatcttcgt tatagttaac taatatcatt aactgatggt
    12181 ctatctctga taggtcaacg tcatatttat ccgcaacggc tttatatctt tcagcatatt
    12241 catatctaac atcattagaa tcatcccact taaagatgag aggaatacct tttttggccg
    12301 cccactcaac aatatgatga ctggttgctg ttacatattt ccgaggtccg cctggcgtat
    12361 aagcatgggg atttacagat attttaggga agctataaaa atcgttatct ggattacaat
    12421 agcctgttgt taaagcatcg ttaatgattt cataacactc ttcaaatagt tgctgttgat
    12481 attcaaccgg gcgattaaaa aaatgcattt catctttttt ttcgcaatca ctaaacccta
    12541 aaataaatct cccttcactt aactgatcca ataagcaagc ttcctccgct atgcggacag
    12601 gatgatgagt tgtaatgatg tgatttaatg aaccaatttt aattttctct gttaaaccga
    12661 gcagaaaacc agaaacagtc agaggagcgc cgacaacacc attatctgaa aaatgatttt
    12721 catacactaa aatctgttca aaattcaact tatcaacata ctccgttatt tcctgcatgc
    12781 gaactatact ttgttcttga acagttgttg aattgatgaa gttaaggaag aacaatccaa
    12841 atttcatatc cattgtctta ttcctttctt tataatagcg aacgttgttt ttctttaaga
    12901 aatggcatga catcagactg gaagagcttc atggaagcaa taatttcgtc tactgttcca
    12961 ttagcttcaa atccacaaca aatatttgat attcctgtag catcaatgtc tttttgaatt
    13021 atgtcaatac attcctgcgg cgttcccacg ggattgattt cgtaactgta atcaatacgg
    13081 cgattagtat ctttatgtcc ttttaataca aagtcacgcc actgcccttt attgaaatca
    13141 taacctcttg tttggtctga atcatcaaaa atagtcgtag cattcacata agaatcatac
    13201 caatgcccca gaaatttccg gcaaatctct ttcgctttaa ttgagtcatg atctacagat
    13261 gttatatatg ataagcaatg gtcgatatta tgaatatcgt gcccatattc ttgagccact
    13321 tcattataaa gctcaagttg tgctttcttt tcgttagtat ttataatcca acttaatatc
    13381 atcggtaggc caaattgagc agcccactca gtcgtcgaag ctgattcagc caccacataa
    13441 accggtgcgc cacctctgct atacgccgcg gggtttactt ttaccttatg gaacttgata
    13501 tgttcattat cagcttccat atatccctct gtcatgccat tctttatcag cccgtaccag
    13561 cattccgcta aggcgcgact gttattcata tctgtgccga atacgcgaaa gtccttgttg
    13621 taaagccctc ggcaaatacc aaaccgaaat cgtccttttg acatttgatc caataaattc
    13681 acatcttcaa gttggcgtac tggatgggct gtgggaagaa caatagcggc agttcctaca
    13741 ttcaattttt tagtcgcgcc aagtaaatat gcagcagcga cataagggtt accaagcaaa
    13801 ccaaactccg tgaaatgatg ctccagtaac catacggtat caaaaccaca ctcctcagag
    13861 atgcgaccta atttaaccaa acgtttcatt acctctgttt gagaaaattg gggaggttgg
    13921 tatgtaagca aaaagtttcc aaatttcata tattttaatt aaaaattaag aatgaagaat
    13981 ttcgagttta tgagtgttac cttcattcat ccttcttcac ccttcattta tatcaactat
    14041 taaatgcttg gtttaagctt aaagcacacc ct
//
